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Protein

N-acetyltransferase 8

Gene

Nat8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acetylates the free alpha-amino group of cysteine S-conjugates to form mercapturic acids. This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6-acetylation of various proteins. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1. May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons (By similarity).By similarity1 Publication

Catalytic activityi

Acetyl-CoA + an L-cysteine-S-conjugate = CoA + an N-acetyl L-cysteine-S-conjugate.

Pathwayi: glutathione metabolism

This protein is involved in the pathway glutathione metabolism, which is part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the pathway glutathione metabolism and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00204.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyltransferase 8 (EC:2.3.1.-)
Alternative name(s):
Acetyltransferase 2
Short name:
ATase2
Camello-like protein 4
Cysteinyl-conjugate N-acetyltransferase (EC:2.3.1.80)
Short name:
CCNAT
Gene namesi
Name:Nat8
Synonyms:Cml4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1915646. Nat8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535CytoplasmicSequence analysisAdd
BLAST
Transmembranei36 – 5621Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini57 – 227171LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227N-acetyltransferase 8PRO_0000284692Add
BLAST

Proteomic databases

PaxDbiQ9JIY7.
PeptideAtlasiQ9JIY7.
PRIDEiQ9JIY7.

PTM databases

iPTMnetiQ9JIY7.
PhosphoSiteiQ9JIY7.

Expressioni

Tissue specificityi

Expressed in brain (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000030004.
CleanExiMM_CML4.
GenevisibleiQ9JIY7. MM.

Interactioni

Subunit structurei

Interacts with PROM1. Interacts with BACE1 (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-1341646.
STRINGi10090.ENSMUSP00000032073.

Structurei

3D structure databases

ProteinModelPortaliQ9JIY7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 217149N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the camello family.1 Publication
Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IS8N. Eukaryota.
ENOG410Y59A. LUCA.
GeneTreeiENSGT00390000009559.
HOGENOMiHOG000261685.
HOVERGENiHBG060476.
InParanoidiQ9JIY7.
OMAiMASFRIR.
OrthoDBiEOG091G0LL0.
PhylomeDBiQ9JIY7.
TreeFamiTF324687.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 2 hits.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFRIRQFQ ERDYKQVVDV FSRGMEEHIP TAFRHLLTLP RTLLLLAVVP
60 70 80 90 100
LAIVLVSGSW FLAVVCIFFL FLFLWFLASK PWKNYVSKCL HTDMADITKS
110 120 130 140 150
YLSVRGSGFW VAESGGQVVG TVAARPVKDP PLGRKQLQLF RLSVSSQHRG
160 170 180 190 200
QGIAKALTRT VLQFARDQGY SDVVLVTGLL QQGAVTLYYS MGFQKTGESF
210 220
VDILTWLVDV SLIHFIYPLP SAQKYEL
Length:227
Mass (Da):25,638
Last modified:October 1, 2000 - v1
Checksum:iD93A2DFB1FB61C7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163317 mRNA. Translation: AAF80486.1.
AK002784 mRNA. Translation: BAB22356.1.
BC019517 mRNA. Translation: AAH19517.1.
CCDSiCCDS20301.1.
RefSeqiNP_075944.1. NM_023455.3.
XP_006506598.1. XM_006506535.2.
XP_006506599.1. XM_006506536.1.
XP_006506600.1. XM_006506537.2.
UniGeneiMm.154782.

Genome annotation databases

EnsembliENSMUST00000032073; ENSMUSP00000032073; ENSMUSG00000030004.
GeneIDi68396.
KEGGimmu:68396.
UCSCiuc009cqh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163317 mRNA. Translation: AAF80486.1.
AK002784 mRNA. Translation: BAB22356.1.
BC019517 mRNA. Translation: AAH19517.1.
CCDSiCCDS20301.1.
RefSeqiNP_075944.1. NM_023455.3.
XP_006506598.1. XM_006506535.2.
XP_006506599.1. XM_006506536.1.
XP_006506600.1. XM_006506537.2.
UniGeneiMm.154782.

3D structure databases

ProteinModelPortaliQ9JIY7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1341646.
STRINGi10090.ENSMUSP00000032073.

PTM databases

iPTMnetiQ9JIY7.
PhosphoSiteiQ9JIY7.

Proteomic databases

PaxDbiQ9JIY7.
PeptideAtlasiQ9JIY7.
PRIDEiQ9JIY7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032073; ENSMUSP00000032073; ENSMUSG00000030004.
GeneIDi68396.
KEGGimmu:68396.
UCSCiuc009cqh.2. mouse.

Organism-specific databases

CTDi9027.
MGIiMGI:1915646. Nat8.

Phylogenomic databases

eggNOGiENOG410IS8N. Eukaryota.
ENOG410Y59A. LUCA.
GeneTreeiENSGT00390000009559.
HOGENOMiHOG000261685.
HOVERGENiHBG060476.
InParanoidiQ9JIY7.
OMAiMASFRIR.
OrthoDBiEOG091G0LL0.
PhylomeDBiQ9JIY7.
TreeFamiTF324687.

Enzyme and pathway databases

UniPathwayiUPA00204.

Miscellaneous databases

ChiTaRSiNat8. mouse.
PROiQ9JIY7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030004.
CleanExiMM_CML4.
GenevisibleiQ9JIY7. MM.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 2 hits.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAT8_MOUSE
AccessioniPrimary (citable) accession number: Q9JIY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.