Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase Hakai

Gene

Cbll1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes ubiquitination of several tyrosine-phosphorylated Src substrates, including CDH1, CTTN and DOK1. Targets CDH1 for endocytosis and degradation.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri109 – 149RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri164 – 190C2H2-typeAdd BLAST27

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI

GO - Biological processi

  • cell-cell adhesion Source: MGI
  • negative regulation of cell adhesion Source: MGI
  • positive regulation of cell migration Source: MGI
  • positive regulation of endocytosis Source: MGI

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Hakai (EC:2.3.2.27)
Alternative name(s):
Casitas B-lineage lymphoma-transforming sequence-like protein 1
E-cadherin binding protein E7
RING-type E3 ubiquitin transferase HakaiCurated
c-Cbl-like protein 1
Gene namesi
Name:Cbll1
Synonyms:Hakai
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:2144842 Cbll1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002840501 – 491E3 ubiquitin-protein ligase HakaiAdd BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201PhosphoserineBy similarity1
Modified residuei285PhosphoserineBy similarity1
Modified residuei290PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JIY2
PaxDbiQ9JIY2
PeptideAtlasiQ9JIY2
PRIDEiQ9JIY2

PTM databases

iPTMnetiQ9JIY2
PhosphoSitePlusiQ9JIY2

Expressioni

Tissue specificityi

Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000020659
CleanExiMM_CBLL1
ExpressionAtlasiQ9JIY2 baseline and differential
GenevisibleiQ9JIY2 MM

Interactioni

Subunit structurei

Homodimer. Interacts with tyrosine-phosphorylated SRC substrates. Component of the WTAP complex composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3 (By similarity).By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi222714, 2 interactors
IntActiQ9JIY2, 27 interactors
MINTiQ9JIY2
STRINGi10090.ENSMUSP00000099038

Structurei

Secondary structure

1491
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni110 – 112Combined sources3
Beta strandi117 – 123Combined sources7
Turni124 – 126Combined sources3
Beta strandi128 – 130Combined sources3
Helixi131 – 139Combined sources9
Turni146 – 148Combined sources3
Beta strandi153 – 159Combined sources7
Helixi160 – 162Combined sources3
Helixi179 – 189Combined sources11
Turni190 – 192Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MQ1NMR-A106-194[»]
3VK6X-ray1.90A106-206[»]
ProteinModelPortaliQ9JIY2
SMRiQ9JIY2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 206HYB domainAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi207 – 481Pro-richAdd BLAST275

Domaini

The HYB domain forms a phosphotyrosine-binding pocket upon dimerization, and mediates as well the recognition of its flanking acidic amino acids.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri109 – 149RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri164 – 190C2H2-typeAdd BLAST27

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2932 Eukaryota
ENOG410XQ6S LUCA
GeneTreeiENSGT00510000047522
HOGENOMiHOG000082498
HOVERGENiHBG057723
InParanoidiQ9JIY2
KOiK15685
OMAiISHPMQH
OrthoDBiEOG091G0AJH
PhylomeDBiQ9JIY2
TreeFamiTF332910

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JIY2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDHTDNELQG TNSSGSLGGL DVRRRIPIKL ISKQASKVKP APRTQRTVSR
60 70 80 90 100
MPAKAPQGDE EGFDYNEEQR YDCKGGELFG NQRRFPGHLF WDFKINILGE
110 120 130 140 150
KDDTPVHFCD KCGLPIKVYG RMIPCKHVFC YDCAILHEKK GDKMCPGCSD
160 170 180 190 200
PVQRIEQCTR GSLFMCSIVQ GCKRTYLSQR DLQAHINHRH MRAGKPVTRA
210 220 230 240 250
SLENVHPPIA PPPTDIPDRF IMPPDKHHMS HIPPKQHIMM PPPPLQHVPH
260 270 280 290 300
EHYNQPHEDI RAPPAELSMA PPPPRSVSQE TFRISTRKHS NLITVPIQDD
310 320 330 340 350
SSSGAREPPP PAPAPAHHHP EYQGQPVVSH PHHIMPPQQH YAPPPPPPPP
360 370 380 390 400
ISHPMPHPPQ AAGTPHLVYS QAPPPPMTSA PPPITPPPGH IIAQMPPYMN
410 420 430 440 450
HPPPGPPPPQ HGGPPVTAPP PHHYNPNSLP QFTEDQGTLS PPFTQPGGMS
460 470 480 490
PGIWPAPRGP PPPPRMQGPP SQTPLPGPHH PDQTRYRPYY Q
Length:491
Mass (Da):54,444
Last modified:October 1, 2000 - v1
Checksum:iAD2A3C188781A7DA
GO
Isoform 2 (identifier: Q9JIY2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-61: Missing.

Show »
Length:490
Mass (Da):54,315
Checksum:i633E8854196D4E83
GO
Isoform 3 (identifier: Q9JIY2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-61: Missing.
     311-374: Missing.
     398-409: Missing.

Show »
Length:414
Mass (Da):46,167
Checksum:iF463812DF2ECAA35
GO
Isoform 4 (identifier: Q9JIY2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     58-61: Missing.
     277-431: Missing.

Show »
Length:332
Mass (Da):37,481
Checksum:i47F62DF8D15B006C
GO

Sequence cautioni

The sequence BAE38522 differs from that shown. Reason: Frameshift at position 470.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02441558 – 61Missing in isoform 4. 1 Publication4
Alternative sequenceiVSP_02441661Missing in isoform 2 and isoform 3. 2 Publications1
Alternative sequenceiVSP_024417277 – 431Missing in isoform 4. 1 PublicationAdd BLAST155
Alternative sequenceiVSP_024418311 – 374Missing in isoform 3. 1 PublicationAdd BLAST64
Alternative sequenceiVSP_024419398 – 409Missing in isoform 3. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167441 mRNA Translation: AAF89617.1
AK049141 mRNA Translation: BAC33568.1
AK166017 mRNA Translation: BAE38522.1 Frameshift.
BC019529 mRNA Translation: AAH19529.1
CCDSiCCDS56834.1 [Q9JIY2-1]
CCDS56835.1 [Q9JIY2-4]
RefSeqiNP_001240776.1, NM_001253847.1 [Q9JIY2-1]
NP_598809.1, NM_134048.2 [Q9JIY2-2]
UniGeneiMm.273270

Genome annotation databases

EnsembliENSMUST00000101499; ENSMUSP00000099038; ENSMUSG00000020659 [Q9JIY2-1]
ENSMUST00000185739; ENSMUSP00000141007; ENSMUSG00000020659 [Q9JIY2-2]
ENSMUST00000188326; ENSMUSP00000139809; ENSMUSG00000020659 [Q9JIY2-4]
GeneIDi104836
KEGGimmu:104836
UCSCiuc007nhl.2 mouse [Q9JIY2-2]
uc007nhm.2 mouse [Q9JIY2-1]
uc007nhn.2 mouse [Q9JIY2-4]

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiHAKAI_MOUSE
AccessioniPrimary (citable) accession number: Q9JIY2
Secondary accession number(s): Q3TMC0, Q8C7W5, Q8VCL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health