Reviewed, UniProtKB/Swiss-Prot Q9JIX3 (FHIT_RAT)

Names and origin

Protein names

Recommended name:
    Bis(5'-adenosyl)-triphosphatase
    EC=3.6.1.29
Alternative name(s):
    Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
    Dinucleosidetriphosphatase
    AP3A hydrolase
      Short name=AP3Aase
    Fragile histidine triad protein

Gene names

Name:

Fhit

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves A-5'-PPP-5'A to yield AMP and ADP By similarity.
Catalytic activity	P(1)-P(3)-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP.
Cofactor	Divalent cations. Magnesium, but manganese and to a lesser extent calcium or cobalt can be substituted; but not zinc, cadmium or nickel By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 HIT domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Manganese
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	diadenosine triphosphate catabolic process Inferred from direct assay. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD nucleus Inferred from direct assay. Source: RGD plasma membrane Inferred from direct assay. Source: RGD
Molecular function	bis(5'-adenosyl)-triphosphatase activity Inferred from direct assay. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 150	149	Bis(5'-adenosyl)-triphosphatase		PRO_0000288473
Regions
	Domain	2 – 109	108	HIT

Sequences

	Sequence		Length	Mass (Da)
	Q9JIX3-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E0C466FB045EE714	FASTA	150	17,348
	10 20 30 40 50 60 MSFKFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLMCP LRPVERFRDL RPDEVADLFQ 70 80 90 100 110 120 VTQRVGTVVE KHFQGTSITF SMQDGPEAGQ TVKHVHVHIL PRKSGDFRRN DNIYDELQKH 130 140 150 DREEEDSPAF WRSEEEMAAE AEVLRAYFQA

References

[1]	"Identification of FHIT gene in rat Fisher 344." Luceri C., De Filippo C., Caderni G., Dolara P. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344. Tissue: Liver.
[2]	Lubec G., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 5-18 AND 49-64, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF170064 mRNA. Translation: AAF89328.1.
IPI	IPI00201106.
RefSeq	NP_068542.1.
UniGene	Rn.45598
3D structure databases
HSSP	HSSP built from PDB template 3FIT based on UniProtKB P49789.
SMR	Q9JIX3. Positions 2-131.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000007167. Rattus norvegicus. [Contig view]
GeneID	60398.
KEGG	rno:60398.
Organism-specific databases
RGD	620448. Fhit.
Phylogenomic databases
HOVERGEN	Q9JIX3.
Enzyme and pathway databases
BRENDA	3.6.1.29. 248.
Family and domain databases
InterPro	IPR011151. His_triad_motif. IPR019808. Histidine_triad_CS. IPR001310. Histidine_triad_HIT. [Graphical view]
Gene3D	G3DSA:3.30.428.10. His_triad_motif. 1 hit.
PANTHER	PTHR23089. HIT. 1 hit.
Pfam	PF01230. HIT. 1 hit. [Graphical view]
PROSITE	PS00892. HIT_1. 1 hit. PS51084. HIT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	612108.

Entry information

Entry name

FHIT_RAT

Accession

Primary (citable) accession number: Q9JIX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)