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Protein

Ras-related protein Ral-B

Gene

Ralb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles (By similarity). Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells (By similarity). Required for suppression of apoptosis (By similarity). In late stages of cytokinesis, upon completion of the bridge formation between dividing cells, mediates exocyst recruitment to the midbody to drive abscission (By similarity).By similarity

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi21 – 29GTPBy similarity9
Nucleotide bindingi68 – 72GTPBy similarity5
Nucleotide bindingi128 – 131GTPBy similarity4
Nucleotide bindingi158 – 160GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis, Cell cycle, Cell division
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Ral-B
Gene namesi
Name:Ralb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1927244. Ralb.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
  • Midbody By similarity

  • Note: During late cytokinesis, enriched at the midbody.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000826991 – 203Ras-related protein Ral-BAdd BLAST203
PropeptideiPRO_0000281351204 – 206Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei203Cysteine methyl esterBy similarity1
Lipidationi203S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Prenylation is essential for membrane localization.By similarity
The farnesylated form confers resistance to the proapoptotic and anti-anchorage-dependent growth effects of some geranylgeranyltransferase I inhibitors.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiQ9JIW9.
MaxQBiQ9JIW9.
PaxDbiQ9JIW9.
PeptideAtlasiQ9JIW9.
PRIDEiQ9JIW9.

PTM databases

iPTMnetiQ9JIW9.
PhosphoSitePlusiQ9JIW9.
SwissPalmiQ9JIW9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004451.
ExpressionAtlasiQ9JIW9. baseline and differential.
GenevisibleiQ9JIW9. MM.

Interactioni

Subunit structurei

Interacts with EXOC2/Sec5 and EXOC8/Exo84 (By similarity). Interacts (via effector domain) with RALBP1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211033. 1 interactor.
IntActiQ9JIW9. 3 interactors.
MINTiMINT-1866131.
STRINGi10090.ENSMUSP00000004565.

Structurei

3D structure databases

ProteinModelPortaliQ9JIW9.
SMRiQ9JIW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 51Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ9JIW9.
KOiK07835.
OMAiTSRMAAN.
OrthoDBiEOG091G0UAU.
PhylomeDBiQ9JIW9.
TreeFamiTF312796.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR028412. Ral.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
PANTHERiPTHR24070. PTHR24070. 1 hit.
PTHR24070:SF348. PTHR24070:SF348. 1 hit.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51421. RAS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JIW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAANKGKSQG SLVLHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS
60 70 80 90 100
YRKKVVLDGE EVQIDILDTA GQEDYAAIRD NYFRSGEGFL LVFSITEHES
110 120 130 140 150
FTATAEFREQ ILRVKSEEDK IPLLVVGNKS DLEERRQVPV DEARGKAEEW
160 170 180 190 200
GVQYVETSAK TRANVDKVFF DLMREIRAKK MSENKDKNGR KSSKSKKSFK

ERCCLL
Length:206
Mass (Da):23,349
Last modified:October 1, 2000 - v1
Checksum:i6CA5994C74221232
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174296 mRNA. Translation: AAF89875.1.
AK017698 mRNA. Translation: BAB30881.1.
AK159993 mRNA. Translation: BAE35543.1.
BC006907 mRNA. Translation: AAH06907.1.
CCDSiCCDS15225.1.
RefSeqiNP_071722.1. NM_022327.5.
XP_006529845.1. XM_006529782.1.
UniGeneiMm.27832.

Genome annotation databases

EnsembliENSMUST00000004565; ENSMUSP00000004565; ENSMUSG00000004451.
GeneIDi64143.
KEGGimmu:64143.
UCSCiuc007cis.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRALB_MOUSE
AccessioniPrimary (citable) accession number: Q9JIW9
Secondary accession number(s): Q3TVS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: October 1, 2000
Last modified: July 5, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families