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Q9JIW5

- SMAD9_MOUSE

UniProt

Q9JIW5 - SMAD9_MOUSE

Protein

Mothers against decapentaplegic homolog 9

Gene

Smad9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (10 May 2005)
      Previous versions | rss
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    Functioni

    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD). Has been shown to be activated by activin type I receptor-like kinases (ALK-2, ALK-3, ALK-6) which stimulate heteromerization between SMAD9 and SMAD4. May play a role in osteoblast differentiation and maturation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi68 – 681ZincBy similarity
    Metal bindingi113 – 1131ZincBy similarity
    Metal bindingi125 – 1251ZincBy similarity
    Metal bindingi130 – 1301ZincBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. BMP signaling pathway Source: MGI
    2. bone development Source: MGI
    3. cartilage development Source: MGI
    4. cellular response to organic cyclic compound Source: MGI
    5. hindbrain development Source: MGI
    6. midbrain development Source: MGI
    7. Mullerian duct regression Source: Ensembl
    8. positive regulation of cell differentiation Source: Ensembl
    9. positive regulation of transcription, DNA-templated Source: Ensembl
    10. protein phosphorylation Source: MGI
    11. response to hypoxia Source: Ensembl
    12. transcription, DNA-templated Source: UniProtKB-KW
    13. transforming growth factor beta receptor signaling pathway Source: InterPro
    14. ureteric bud development Source: UniProtKB

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_220505. Signaling by BMP.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mothers against decapentaplegic homolog 9
    Short name:
    MAD homolog 9
    Short name:
    Mothers against DPP homolog 9
    Alternative name(s):
    SMAD family member 9
    Short name:
    SMAD 9
    Short name:
    Smad9
    Smad8
    Gene namesi
    Name:Smad9
    Synonyms:Madh8, Madh9, Smad8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1859993. Smad9.

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI
    3. transcription factor complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 430430Mothers against decapentaplegic homolog 9PRO_0000090876Add
    BLAST

    Post-translational modificationi

    Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase and activin type I receptor-like kinases (ALK-2, ALK-3 and ALK-6).

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9JIW5.
    PaxDbiQ9JIW5.
    PRIDEiQ9JIW5.

    PTM databases

    PhosphoSiteiQ9JIW5.

    Expressioni

    Gene expression databases

    BgeeiQ9JIW5.
    CleanExiMM_SMAD9.
    GenevestigatoriQ9JIW5.

    Interactioni

    Subunit structurei

    Interaction with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit By similarity.By similarity

    Protein-protein interaction databases

    BioGridi207765. 10 interactions.
    IntActiQ9JIW5. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JIW5.
    SMRiQ9JIW5. Positions 13-136, 233-430.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 140125MH1PROSITE-ProRule annotationAdd
    BLAST
    Domaini236 – 430195MH2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi43 – 497Poly-Lys

    Sequence similaritiesi

    Belongs to the dwarfin/SMAD family.Curated
    Contains 1 MH1 (MAD homology 1) domain.PROSITE-ProRule annotation
    Contains 1 MH2 (MAD homology 2) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG330956.
    GeneTreeiENSGT00600000084186.
    HOGENOMiHOG000286018.
    HOVERGENiHBG053353.
    InParanoidiQ9JIW5.
    KOiK16791.
    OMAiFQQPPCP.
    OrthoDBiEOG7W1540.
    PhylomeDBiQ9JIW5.
    TreeFamiTF314923.

    Family and domain databases

    Gene3Di2.60.200.10. 1 hit.
    3.90.520.10. 1 hit.
    InterProiIPR013790. Dwarfin.
    IPR003619. MAD_homology1_Dwarfin-type.
    IPR013019. MAD_homology_MH1.
    IPR017855. SMAD_dom-like.
    IPR001132. SMAD_dom_Dwarfin-type.
    IPR008984. SMAD_FHA_domain.
    [Graphical view]
    PANTHERiPTHR13703. PTHR13703. 1 hit.
    PfamiPF03165. MH1. 1 hit.
    PF03166. MH2. 1 hit.
    [Graphical view]
    SMARTiSM00523. DWA. 1 hit.
    SM00524. DWB. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    SSF56366. SSF56366. 1 hit.
    PROSITEiPS51075. MH1. 1 hit.
    PS51076. MH2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JIW5-1 [UniParc]FASTAAdd to Basket

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    MHPSTPISSL FSFTSPAVKR LLGWKQGDEE EKWAEKAVDS LVKKLKKKKG    50
    AMDELERALS CPGQPSKCVT IPRSLDGRLQ VSHRKGLPHV IYCRVWRWPD 100
    LQSHHELKPL ECCEFPFGSK QKEVCINPYH YRRVETPVLP PVLVPRHSEY 150
    NPQLSLLAKF RSASLHSEPL MPHNATYPDS FQQSLCPAPP SSPGHVFPQS 200
    PCPTSYPHSP GSPSESDSPY QHSDFRPVCY EEPQHWCSVA YYELNNRVGE 250
    TFQASSRSVL IDGFTDPSNN RNRFCLGLLS NVNRNSTIEN TRRHIGKGVH 300
    LYYVGGEVYA ECVSDSSIFV QSRNCNYQHG FHPATVCKIP SGCSLKVFNN 350
    QLFAQLLAQS VHHGFEVVYE LTKMCTIRMS FVKGWGAEYH RQDVTSTPCW 400
    IEIHLHGPLQ WLDKVLTQMG SPHNPISSVS 430
    Length:430
    Mass (Da):48,636
    Last modified:May 10, 2005 - v2
    Checksum:i3B15EC51D6A594E5
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti214 – 2152Missing.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF175408 mRNA. Translation: AAF77079.2.
    AY145520 mRNA. Translation: AAN85445.1.
    CCDSiCCDS17354.1.
    RefSeqiNP_062356.3. NM_019483.5.
    XP_006501791.1. XM_006501728.1.
    UniGeneiMm.244353.

    Genome annotation databases

    EnsembliENSMUST00000029371; ENSMUSP00000029371; ENSMUSG00000027796.
    GeneIDi55994.
    KEGGimmu:55994.
    UCSCiuc008pfv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF175408 mRNA. Translation: AAF77079.2 .
    AY145520 mRNA. Translation: AAN85445.1 .
    CCDSi CCDS17354.1.
    RefSeqi NP_062356.3. NM_019483.5.
    XP_006501791.1. XM_006501728.1.
    UniGenei Mm.244353.

    3D structure databases

    ProteinModelPortali Q9JIW5.
    SMRi Q9JIW5. Positions 13-136, 233-430.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207765. 10 interactions.
    IntActi Q9JIW5. 1 interaction.

    PTM databases

    PhosphoSitei Q9JIW5.

    Proteomic databases

    MaxQBi Q9JIW5.
    PaxDbi Q9JIW5.
    PRIDEi Q9JIW5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029371 ; ENSMUSP00000029371 ; ENSMUSG00000027796 .
    GeneIDi 55994.
    KEGGi mmu:55994.
    UCSCi uc008pfv.1. mouse.

    Organism-specific databases

    CTDi 4093.
    MGIi MGI:1859993. Smad9.

    Phylogenomic databases

    eggNOGi NOG330956.
    GeneTreei ENSGT00600000084186.
    HOGENOMi HOG000286018.
    HOVERGENi HBG053353.
    InParanoidi Q9JIW5.
    KOi K16791.
    OMAi FQQPPCP.
    OrthoDBi EOG7W1540.
    PhylomeDBi Q9JIW5.
    TreeFami TF314923.

    Enzyme and pathway databases

    Reactomei REACT_220505. Signaling by BMP.

    Miscellaneous databases

    ChiTaRSi SMAD9. mouse.
    NextBioi 311730.
    PROi Q9JIW5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JIW5.
    CleanExi MM_SMAD9.
    Genevestigatori Q9JIW5.

    Family and domain databases

    Gene3Di 2.60.200.10. 1 hit.
    3.90.520.10. 1 hit.
    InterProi IPR013790. Dwarfin.
    IPR003619. MAD_homology1_Dwarfin-type.
    IPR013019. MAD_homology_MH1.
    IPR017855. SMAD_dom-like.
    IPR001132. SMAD_dom_Dwarfin-type.
    IPR008984. SMAD_FHA_domain.
    [Graphical view ]
    PANTHERi PTHR13703. PTHR13703. 1 hit.
    Pfami PF03165. MH1. 1 hit.
    PF03166. MH2. 1 hit.
    [Graphical view ]
    SMARTi SM00523. DWA. 1 hit.
    SM00524. DWB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    SSF56366. SSF56366. 1 hit.
    PROSITEi PS51075. MH1. 1 hit.
    PS51076. MH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse Smad8 phosphorylation downstream of BMP receptors ALK-2, ALK-3, and ALK-6 induces its association with Smad4 and transcriptional activity."
      Kawai S., Faucheu C., Gallea S., Spinella-Jaegle S., Atfi A., Baron R., Roman-Roman S.
      Biochem. Biophys. Res. Commun. 271:682-687(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, VARIANT 214-SER-GLU-215 DEL.
      Tissue: Embryo.
    2. Kawai S., Roman-Roman S.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 115 AND 135.
    3. "Mouse Smad8 protein mRNA alternative form (6-bp insert in linker region)."
      Kawai S., Roman-Roman S.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.

    Entry informationi

    Entry nameiSMAD9_MOUSE
    AccessioniPrimary (citable) accession number: Q9JIW5
    Secondary accession number(s): Q8CFF9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3