Mothers against decapentaplegic homolog 9

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Q9JIW5 (SMAD9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mothers against decapentaplegic homolog 9
Short name=MAD homolog 9
Short name=Mothers against DPP homolog 9

Alternative name(s):
SMAD family member 9
Short name=SMAD 9
Short name=Smad9
Smad8

Gene names

Name:	Smad9
Synonyms:	Madh8, Madh9, Smad8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD). Has been shown to be activated by activin type I receptor-like kinases (ALK-2, ALK-3, ALK-6) which stimulate heteromerization between SMAD9 and SMAD4. May play a role in osteoblast differentiation and maturation.
Subunit structure	Interaction with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit By similarity.
Subcellular location	Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Post-translational modification	Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase and activin type I receptor-like kinases (ALK-2, ALK-3 and ALK-6).
Sequence similarities	Belongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Inferred from direct assay PubMed 18590716. Source: MGI Mullerian duct regression Inferred from electronic annotation. Source: Compara bone development Inferred from genetic interaction PubMed 19224984. Source: MGI cartilage development Inferred from genetic interaction PubMed 19224984. Source: MGI cellular response to organic cyclic compound Inferred from direct assay PubMed 19103752. Source: MGI hindbrain development Inferred from mutant phenotype PubMed 15899870. Source: MGI midbrain development Inferred from mutant phenotype PubMed 15899870. Source: MGI positive regulation of cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara protein phosphorylation Inferred from direct assay PubMed 18776146. Source: MGI response to hypoxia Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: InterPro ureteric bud development Inferred from expression pattern PubMed 14656760. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay PubMed 19103752. Source: MGI nucleus Inferred from direct assay PubMed 18776146 PubMed 19103752 PubMed 19793887. Source: MGI transcription factor complex Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 430

430

Mothers against decapentaplegic homolog 9

PRO_0000090876

Regions

Domain

16 – 140

125

MH1

Domain

236 – 430

195

MH2

Compositional bias

43 – 49

Poly-Lys

Sites

Metal binding

Zinc By similarity

Metal binding

113

Zinc By similarity

Metal binding

125

Zinc By similarity

Metal binding

130

Zinc By similarity

Natural variations

Natural variant

214 – 215

Missing.

Sequences

Sequence

Length

Mass (Da)

Tools

Q9JIW5 [UniParc].

Last modified May 10, 2005. Version 2.
Checksum: 3B15EC51D6A594E5
Show »

FASTA

430

48,636

        10         20         30         40         50         60 
MHPSTPISSL FSFTSPAVKR LLGWKQGDEE EKWAEKAVDS LVKKLKKKKG AMDELERALS 

        70         80         90        100        110        120 
CPGQPSKCVT IPRSLDGRLQ VSHRKGLPHV IYCRVWRWPD LQSHHELKPL ECCEFPFGSK 

       130        140        150        160        170        180 
QKEVCINPYH YRRVETPVLP PVLVPRHSEY NPQLSLLAKF RSASLHSEPL MPHNATYPDS 

       190        200        210        220        230        240 
FQQSLCPAPP SSPGHVFPQS PCPTSYPHSP GSPSESDSPY QHSDFRPVCY EEPQHWCSVA 

       250        260        270        280        290        300 
YYELNNRVGE TFQASSRSVL IDGFTDPSNN RNRFCLGLLS NVNRNSTIEN TRRHIGKGVH 

       310        320        330        340        350        360 
LYYVGGEVYA ECVSDSSIFV QSRNCNYQHG FHPATVCKIP SGCSLKVFNN QLFAQLLAQS 

       370        380        390        400        410        420 
VHHGFEVVYE LTKMCTIRMS FVKGWGAEYH RQDVTSTPCW IEIHLHGPLQ WLDKVLTQMG 

       430 
SPHNPISSVS

« Hide

References

[1]	"Mouse Smad8 phosphorylation downstream of BMP receptors ALK-2, ALK-3, and ALK-6 induces its association with Smad4 and transcriptional activity." Kawai S., Faucheu C., Gallea S., Spinella-Jaegle S., Atfi A., Baron R., Roman-Roman S. Biochem. Biophys. Res. Commun. 271:682-687(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, VARIANT 214-SER-GLU-215 DEL. Tissue: Embryo.
[2]	Kawai S., Roman-Roman S. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 115 AND 135.
[3]	"Mouse Smad8 protein mRNA alternative form (6-bp insert in linker region)." Kawai S., Roman-Roman S. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF175408 mRNA. Translation: AAF77079.2. AY145520 mRNA. Translation: AAN85445.1.
IPI	IPI00310561.
RefSeq	NP_062356.3. NM_019483.4.
UniGene	Mm.244353.
3D structure databases
ProteinModelPortal	Q9JIW5.
SMR	Q9JIW5. Positions 13-136, 233-430.
ModBase	Search...
PTM databases
PhosphoSite	Q9JIW5.
Proteomic databases
PaxDb	Q9JIW5.
PRIDE	Q9JIW5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029371; ENSMUSP00000029371; ENSMUSG00000027796.
GeneID	55994.
KEGG	mmu:55994.
Organism-specific databases
CTD	4093.
MGI	MGI:1859993. Smad9.
Phylogenomic databases
eggNOG	NOG330956.
GeneTree	ENSGT00600000084186.
HOGENOM	HOG000286018.
HOVERGEN	HBG053353.
InParanoid	Q9JIW5.
KO	K16791.
OMA	SDFRPVC.
OrthoDB	EOG4HDSTH.
Gene expression databases
ArrayExpress	Q9JIW5.
Bgee	Q9JIW5.
CleanEx	MM_SMAD9.
Genevestigator	Q9JIW5.
GermOnline	ENSMUSG00000027796. Mus musculus.
Family and domain databases
Gene3D	2.60.200.10. 1 hit. 3.90.520.10. 1 hit.
InterPro	IPR013790. Dwarfin. IPR003619. MAD_homology1_Dwarfin-type. IPR013019. MAD_homology_MH1. IPR017855. SMAD_dom-like. IPR001132. SMAD_dom_Dwarfin-type. IPR008984. SMAD_FHA_domain. [Graphical view]
PANTHER	PTHR13703. PTHR13703. 1 hit.
Pfam	PF03165. MH1. 1 hit. PF03166. MH2. 1 hit. [Graphical view]
SMART	SM00523. DWA. 1 hit. SM00524. DWB. 1 hit. [Graphical view]
SUPFAM	SSF56366. MAD_MH1. 1 hit. SSF49879. SMAD_FHA. 1 hit.
PROSITE	PS51075. MH1. 1 hit. PS51076. MH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SMAD9. mouse.
NextBio	311730.
SOURCE	Search...

Entry information

Entry name

SMAD9_MOUSE

Accession

Primary (citable) accession number: Q9JIW5
Secondary accession number(s): Q8CFF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 10, 2005
Last modified:	April 3, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents