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Protein

DNA-directed DNA/RNA polymerase mu

Gene

Polm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.2 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi241Sodium; via carbonyl oxygenBy similarity1
Metal bindingi243Sodium; via carbonyl oxygenBy similarity1
Metal bindingi330Magnesium1
Metal bindingi332Magnesium1
Metal bindingi420MagnesiumBy similarity1
Sitei435Responsible for the low discrimination between dNTP and rNTPBy similarity1

GO - Molecular functioni

GO - Biological processi

  • B cell differentiation Source: MGI
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • somatic hypermutation of immunoglobulin genes Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5693571. Nonhomologous End-Joining (NHEJ).

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed DNA/RNA polymerase mu (EC:2.7.7.7)
Short name:
Pol Mu
Alternative name(s):
Terminal transferase
Gene namesi
Name:Polm
Synonyms:polmu
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1860191. Polm.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187881 – 496DNA-directed DNA/RNA polymerase muAdd BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JIW4.
PaxDbiQ9JIW4.
PRIDEiQ9JIW4.

PTM databases

iPTMnetiQ9JIW4.
PhosphoSitePlusiQ9JIW4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020474.
CleanExiMM_POLM.
ExpressionAtlasiQ9JIW4. baseline and differential.
GenevisibleiQ9JIW4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020767.

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi142 – 144Combined sources3
Helixi154 – 169Combined sources16
Helixi173 – 188Combined sources16
Helixi196 – 199Combined sources4
Helixi207 – 219Combined sources13
Helixi223 – 230Combined sources8
Helixi232 – 241Combined sources10
Helixi248 – 256Combined sources9
Helixi262 – 266Combined sources5
Helixi274 – 281Combined sources8
Helixi283 – 287Combined sources5
Helixi292 – 307Combined sources16
Beta strandi314 – 317Combined sources4
Helixi319 – 322Combined sources4
Beta strandi326 – 336Combined sources11
Turni340 – 345Combined sources6
Helixi346 – 356Combined sources11
Beta strandi360 – 366Combined sources7
Beta strandi389 – 397Combined sources9
Beta strandi414 – 423Combined sources10
Helixi429 – 437Combined sources9
Helixi440 – 454Combined sources15
Helixi477 – 483Combined sources7
Helixi491 – 493Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IHMX-ray2.40A/B137-496[»]
ProteinModelPortaliQ9JIW4.
SMRiQ9JIW4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JIW4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 122BRCTPROSITE-ProRule annotationAdd BLAST100

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni323 – 332Involved in ssDNA binding10

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
GeneTreeiENSGT00530000063002.
HOGENOMiHOG000263600.
HOVERGENiHBG003670.
InParanoidiQ9JIW4.
KOiK03513.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
cd00141. NT_POLXc. 1 hit.
Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR027249. DNA/RNApol_mu.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
IPR001726. TdT/Mu.
[Graphical view]
PfamiPF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000817. DNA_NT. 1 hit.
PIRSF501176. DNApol_mu. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTiSM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPKRRRVRA GSPHSAVASS TPPSVVRFPD VAIYLAEPRM GRSRRAFLTR
60 70 80 90 100
LARSKGFRVL DAYSSKVTHV VMEGTSAKEA ICWQKNMDAL PTGCPQPALL
110 120 130 140 150
DISWFTESMA AGQPVPEEGR HHLEVAEPRK EPPVSASMPA YACQRPSPLT
160 170 180 190 200
HHNTLLSEAL ETLAEAAGFE ANEGRLLSFS RAASVLKSLP CPVASLSQLH
210 220 230 240 250
GLPYFGEHST RVIQELLEHG TCEEVKQVRC SERYQTMKLF TQVFGVGVKT
260 270 280 290 300
ANRWYQEGLR TLDELREQPQ RLTQQQKAGL QYYQDLSTPV RRADAEALQQ
310 320 330 340 350
LIEAAVRQTL PGATVTLTGG FRRGKLQGHD VDFLITHPEE GQEVGLLPKV
360 370 380 390 400
MSCLQSQGLV LYHQYHRSHL ADSAHNLRQR SSTMDAFERS FCILGLPQPQ
410 420 430 440 450
QAALAGALPP CPTWKAVRVD LVVTPSSQFP FALLGWTGSQ FFERELRRFS
460 470 480 490
RQEKGLWLNS HGLFDPEQKR VFHATSEEDV FRLLGLKYLP PEQRNA
Length:496
Mass (Da):55,490
Last modified:October 3, 2012 - v2
Checksum:iD087636E30C27127
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18A → D in AAF27552 (PubMed:10982892).Curated1
Sequence conflicti386A → V in CAB71154 (PubMed:10747040).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176098 mRNA. Translation: AAF27552.1.
AJ251804 mRNA. Translation: CAB71154.1.
AL627069 Genomic DNA. No translation available.
CH466574 Genomic DNA. Translation: EDL40561.1.
CCDSiCCDS24405.1.
RefSeqiNP_059097.2. NM_017401.2.
UniGeneiMm.260194.

Genome annotation databases

EnsembliENSMUST00000020767; ENSMUSP00000020767; ENSMUSG00000020474.
GeneIDi54125.
KEGGimmu:54125.
UCSCiuc007hxf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176098 mRNA. Translation: AAF27552.1.
AJ251804 mRNA. Translation: CAB71154.1.
AL627069 Genomic DNA. No translation available.
CH466574 Genomic DNA. Translation: EDL40561.1.
CCDSiCCDS24405.1.
RefSeqiNP_059097.2. NM_017401.2.
UniGeneiMm.260194.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IHMX-ray2.40A/B137-496[»]
ProteinModelPortaliQ9JIW4.
SMRiQ9JIW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020767.

PTM databases

iPTMnetiQ9JIW4.
PhosphoSitePlusiQ9JIW4.

Proteomic databases

EPDiQ9JIW4.
PaxDbiQ9JIW4.
PRIDEiQ9JIW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020767; ENSMUSP00000020767; ENSMUSG00000020474.
GeneIDi54125.
KEGGimmu:54125.
UCSCiuc007hxf.2. mouse.

Organism-specific databases

CTDi27434.
MGIiMGI:1860191. Polm.

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
GeneTreeiENSGT00530000063002.
HOGENOMiHOG000263600.
HOVERGENiHBG003670.
InParanoidiQ9JIW4.
KOiK03513.

Enzyme and pathway databases

ReactomeiR-MMU-5693571. Nonhomologous End-Joining (NHEJ).

Miscellaneous databases

EvolutionaryTraceiQ9JIW4.
PROiQ9JIW4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020474.
CleanExiMM_POLM.
ExpressionAtlasiQ9JIW4. baseline and differential.
GenevisibleiQ9JIW4. MM.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
cd00141. NT_POLXc. 1 hit.
Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR027249. DNA/RNApol_mu.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
IPR001726. TdT/Mu.
[Graphical view]
PfamiPF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000817. DNA_NT. 1 hit.
PIRSF501176. DNApol_mu. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTiSM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPOLM_MOUSE
AccessioniPrimary (citable) accession number: Q9JIW4
Secondary accession number(s): G5E840, Q9JJW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

DPOLM has a reduced ability to distinguish dNTP and rNTP as substrates, and elongates them on DNA primer strand with a similar efficiency. It is able to polymerize nucleotides on RNA primer strands (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.