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Q9JIW4 (DPOLM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed DNA/RNA polymerase mu

Short name=Pol Mu
EC=2.7.7.7
Alternative name(s):
Terminal transferase
Gene names
Name:Polm
Synonyms:polmu
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination. Ref.5 Ref.6

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactor

Magnesium. Ref.7

Subcellular location

Nucleus By similarity.

Miscellaneous

DPOLM has a reduced ability to distinguish dNTP and rNTP as substrates, and elongates them on DNA primer strand with a similar efficiency. It is able to polymerize nucleotides on RNA primer strands By similarity.

Sequence similarities

Belongs to the DNA polymerase type-X family.

Contains 1 BRCT domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496DNA-directed DNA/RNA polymerase mu
PRO_0000218788

Regions

Domain23 – 122100BRCT
Region323 – 33210Involved in ssDNA binding

Sites

Metal binding2411Sodium; via carbonyl oxygen By similarity
Metal binding2431Sodium; via carbonyl oxygen By similarity
Metal binding3301Magnesium
Metal binding3321Magnesium
Metal binding4201Magnesium By similarity
Site4351Responsible for the low discrimination between dNTP and rNTP By similarity

Amino acid modifications

Modified residue121Phosphoserine By similarity

Experimental info

Sequence conflict181A → D in AAF27552. Ref.1
Sequence conflict3861A → V in CAB71154. Ref.2

Secondary structure

................................................ 496
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9JIW4 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: D087636E30C27127

FASTA49655,490
        10         20         30         40         50         60 
MLPKRRRVRA GSPHSAVASS TPPSVVRFPD VAIYLAEPRM GRSRRAFLTR LARSKGFRVL 

        70         80         90        100        110        120 
DAYSSKVTHV VMEGTSAKEA ICWQKNMDAL PTGCPQPALL DISWFTESMA AGQPVPEEGR 

       130        140        150        160        170        180 
HHLEVAEPRK EPPVSASMPA YACQRPSPLT HHNTLLSEAL ETLAEAAGFE ANEGRLLSFS 

       190        200        210        220        230        240 
RAASVLKSLP CPVASLSQLH GLPYFGEHST RVIQELLEHG TCEEVKQVRC SERYQTMKLF 

       250        260        270        280        290        300 
TQVFGVGVKT ANRWYQEGLR TLDELREQPQ RLTQQQKAGL QYYQDLSTPV RRADAEALQQ 

       310        320        330        340        350        360 
LIEAAVRQTL PGATVTLTGG FRRGKLQGHD VDFLITHPEE GQEVGLLPKV MSCLQSQGLV 

       370        380        390        400        410        420 
LYHQYHRSHL ADSAHNLRQR SSTMDAFERS FCILGLPQPQ QAALAGALPP CPTWKAVRVD 

       430        440        450        460        470        480 
LVVTPSSQFP FALLGWTGSQ FFERELRRFS RQEKGLWLNS HGLFDPEQKR VFHATSEEDV 

       490 
FRLLGLKYLP PEQRNA 

« Hide

References

« Hide 'large scale' references
[1]"Two novel human and mouse DNA polymerases of the polX family."
Aoufouchi S., Flatter E., Dahan A., Faili A., Bertocci B., Storck S., Delbos F., Cocea L., Gupta N., Weill J.-C., Reynaud C.-A.
Nucleic Acids Res. 28:3684-3693(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"DNA polymerase mu (Pol mu), homologous to TdT, could act as a DNA mutator in eukaryotic cells."
Dominguez O., Ruiz J.F., Lain de Lera T., Garcia-Diaz M., Gonzalez M.A., Kirchhoff T., Martinez-A C., Bernad A., Blanco L.
EMBO J. 19:1731-1742(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CD-1.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Nonoverlapping functions of DNA polymerases mu, lambda, and terminal deoxynucleotidyltransferase during immunoglobulin V(D)J recombination in vivo."
Bertocci B., De Smet A., Weill J.C., Reynaud C.A.
Immunity 25:31-41(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Involvement of DNA polymerase mu in the repair of a specific subset of DNA double-strand breaks in mammalian cells."
Capp J.P., Boudsocq F., Besnard A.G., Lopez B.S., Cazaux C., Hoffmann J.S., Canitrot Y.
Nucleic Acids Res. 35:3551-3560(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Structural insight into the substrate specificity of DNA Polymerase mu."
Moon A.F., Garcia-Diaz M., Bebenek K., Davis B.J., Zhong X., Ramsden D.A., Kunkel T.A., Pedersen L.C.
Nat. Struct. Mol. Biol. 14:45-53(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 137-496 IN COMPLEX WITH DNA, MAGNESIUM-BINDING SITES, COFACTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF176098 mRNA. Translation: AAF27552.1.
AJ251804 mRNA. Translation: CAB71154.1.
AL627069 Genomic DNA. No translation available.
CH466574 Genomic DNA. Translation: EDL40561.1.
RefSeqNP_059097.2. NM_017401.2.
UniGeneMm.260194.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2IHMX-ray2.40A/B137-496[»]
ProteinModelPortalQ9JIW4.
SMRQ9JIW4. Positions 21-124, 137-496.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9JIW4.

Proteomic databases

PRIDEQ9JIW4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020767; ENSMUSP00000020767; ENSMUSG00000020474.
GeneID54125.
KEGGmmu:54125.
UCSCuc007hxf.2. mouse.

Organism-specific databases

CTD27434.
MGIMGI:1860191. Polm.

Phylogenomic databases

eggNOGCOG1796.
GeneTreeENSGT00530000063002.
HOGENOMHOG000263600.
HOVERGENHBG003670.
KOK03513.

Gene expression databases

ArrayExpressQ9JIW4.
CleanExMM_POLM.
GenevestigatorQ9JIW4.

Family and domain databases

Gene3D1.10.150.110. 1 hit.
3.40.50.10190. 1 hit.
InterProIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR027249. DNA/RNApol_mu.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR001726. TdT/Mu.
[Graphical view]
PANTHERPTHR11276:SF6. PTHR11276:SF6. 1 hit.
PfamPF14792. DNA_pol_B_palm. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
[Graphical view]
PIRSFPIRSF000817. DNA_NT. 1 hit.
PIRSF501176. DNApol_mu. 1 hit.
PRINTSPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTSM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
SSF81585. SSF81585. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9JIW4.
NextBio310917.
PROQ9JIW4.
SOURCESearch...

Entry information

Entry nameDPOLM_MOUSE
AccessionPrimary (citable) accession number: Q9JIW4
Secondary accession number(s): G5E840, Q9JJW9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot