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Protein

Transducin-like enhancer protein 3

Gene

Tle3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-RNO-201722. Formation of the beta-catenin:TCF transactivating complex.
R-RNO-3769402. Deactivation of the beta-catenin transactivating complex.
R-RNO-4641265. Repression of WNT target genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transducin-like enhancer protein 3
Short name:
rTLE3
Gene namesi
Name:Tle3
Synonyms:Esp3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi620292. Tle3.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512821 – 764Transducin-like enhancer protein 3Add BLAST764

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei203PhosphoserineCombined sources1
Modified residuei207PhosphoserineBy similarity1
Modified residuei211PhosphoserineBy similarity1
Modified residuei217PhosphoserineCombined sources1
Modified residuei232N6-acetyllysineBy similarity1
Modified residuei240PhosphoserineBy similarity1
Modified residuei245PhosphoserineCombined sources1
Modified residuei259PhosphothreonineCombined sources1
Modified residuei263PhosphoserineCombined sources1
Modified residuei267PhosphoserineCombined sources1
Modified residuei275N6-acetyllysineBy similarity1
Modified residuei286PhosphoserineBy similarity1
Modified residuei312PhosphothreonineBy similarity1
Modified residuei317PhosphoserineBy similarity1
Modified residuei319PhosphothreonineBy similarity1
Modified residuei321PhosphothreonineBy similarity1
Modified residuei328PhosphothreonineBy similarity1
Modified residuei334PhosphothreonineBy similarity1
Modified residuei410PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9JIT3.
PRIDEiQ9JIT3.

PTM databases

iPTMnetiQ9JIT3.
PhosphoSitePlusiQ9JIT3.

Expressioni

Tissue specificityi

Highly expressed in adrenal gland, small intestine, kidney, lung, ovary and thyroid. Detected at lower levels in pituitary, hippocampus, cortex, cerebellum and testis.

Inductioni

By kainic acid in the dentate gyrus.

Gene expression databases

BgeeiENSRNOG00000013013.
GenevisibleiQ9JIT3. RN.

Interactioni

Subunit structurei

Homotetramer and heterooligomer with other family members. Binds LEF1, TCF7, TCF7L1, TCF7L2 and FOXA2. Interacts with XIAP/BIRC4 and TCF7L2/TCF4. Interacts with TBX18 (via engrailed homology 1 repressor motif), leading to decreased of TBX18 transcriptional activity.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018467.

Structurei

3D structure databases

ProteinModelPortaliQ9JIT3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati476 – 514WD 1Add BLAST39
Repeati522 – 561WD 2Add BLAST40
Repeati566 – 605WD 3Add BLAST40
Repeati608 – 647WD 4Add BLAST40
Repeati649 – 688WD 5Add BLAST40
Repeati690 – 729WD 6Add BLAST40
Repeati731 – 763WD 7Add BLAST33

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 131Q domainBy similarityAdd BLAST131
Regioni132 – 199GP domainBy similarityAdd BLAST68
Regioni200 – 268CcN domainBy similarityAdd BLAST69
Regioni269 – 444SP domainBy similarityAdd BLAST176

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi225 – 228Nuclear localization signalSequence analysis4

Domaini

WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.1 Publication

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
GeneTreeiENSGT00550000074465.
HOGENOMiHOG000293211.
HOVERGENiHBG004689.
InParanoidiQ9JIT3.
OMAiHDLDHRE.
OrthoDBiEOG091G181Y.
PhylomeDBiQ9JIT3.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005617. Groucho/TLE_N.
IPR009146. Groucho_enhance.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR01850. GROUCHOFAMLY.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPQGRHPAP HQPGQPGFKF TVAESCDRIK DEFQFLQAQY HSLKVEYDKL
60 70 80 90 100
ANEKTEMQRH YVMYYEMSYG LNIEMHKQTE IAKRLNTILA QIMPFLSQEH
110 120 130 140 150
QQQVAQAVER AKQVTMTELN AIIGQQQLQA QHLSHATHGP PVQLPPHPSG
160 170 180 190 200
LQPPGIPPVT GSSSGLLALG ALGSQAHLAV KDEKNHHELD HRERESSTNN
210 220 230 240 250
SVSPSESLRA SEKHRGSADY SMEAKKRKAE EKDSLSRYDS DGDKSDDLVV
260 270 280 290 300
DVSNEDPATP RVSPAHSPPE NGLDKARGLK KDAPTSPASV ASSSSTPSSK
310 320 330 340 350
TKDLGHNDKS STPGLKSNTP TPRNDAPTPG TSTTPGLRSM PGKPPGMDPI
360 370 380 390 400
ASALRTPISL TSSYAAPFAM MSHHEMNGSL TSPSAYAGLH NIPSQMSAAA
410 420 430 440 450
AAAAAAYGRS PMVGFDPHPP MRATGLPSSL ASIPGGKPAY SFHVSADGQM
460 470 480 490 500
QPVPFPHDAL AGPGIPRHAR QINTLSHGEV VCAVTISNPT RHVYTGGKGC
510 520 530 540 550
VKIWDISQPG SKSPISQLDC LNRDNYIRSC KLLPDGRTLI VGGEASTLTI
560 570 580 590 600
WDLASPTPRI KAELTSSAPA CYALAISPDA KVCFSCCSDG NIAVWDLHNQ
610 620 630 640 650
TLVRQFQGHT DGASCIDISH DGTKLWTGGL DNTVRSWDLR EGRQLQQHDF
660 670 680 690 700
TSQIFSLGYC PTGEWLAVGM ESSNVEVLHH TKPDKYQLHL HESCVLSLKF
710 720 730 740 750
AYCGKWFVST GKDNLLNAWR TPYGASIFQS KESSSVLSCD ISADDKYIVT
760
GSGDKKATVY EVIY
Length:764
Mass (Da):82,644
Last modified:October 1, 2000 - v1
Checksum:i7C5EAAEC9AA1A2DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF186092 mRNA. Translation: AAF75590.1.
RefSeqiNP_445852.1. NM_053400.1.
UniGeneiRn.24106.

Genome annotation databases

EnsembliENSRNOT00000018467; ENSRNOP00000018467; ENSRNOG00000013013.
GeneIDi84424.
KEGGirno:84424.
UCSCiRGD:620292. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF186092 mRNA. Translation: AAF75590.1.
RefSeqiNP_445852.1. NM_053400.1.
UniGeneiRn.24106.

3D structure databases

ProteinModelPortaliQ9JIT3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018467.

PTM databases

iPTMnetiQ9JIT3.
PhosphoSitePlusiQ9JIT3.

Proteomic databases

PaxDbiQ9JIT3.
PRIDEiQ9JIT3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018467; ENSRNOP00000018467; ENSRNOG00000013013.
GeneIDi84424.
KEGGirno:84424.
UCSCiRGD:620292. rat.

Organism-specific databases

CTDi7090.
RGDi620292. Tle3.

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
GeneTreeiENSGT00550000074465.
HOGENOMiHOG000293211.
HOVERGENiHBG004689.
InParanoidiQ9JIT3.
OMAiHDLDHRE.
OrthoDBiEOG091G181Y.
PhylomeDBiQ9JIT3.

Enzyme and pathway databases

ReactomeiR-RNO-201722. Formation of the beta-catenin:TCF transactivating complex.
R-RNO-3769402. Deactivation of the beta-catenin transactivating complex.
R-RNO-4641265. Repression of WNT target genes.

Miscellaneous databases

PROiQ9JIT3.

Gene expression databases

BgeeiENSRNOG00000013013.
GenevisibleiQ9JIT3. RN.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005617. Groucho/TLE_N.
IPR009146. Groucho_enhance.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR01850. GROUCHOFAMLY.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLE3_RAT
AccessioniPrimary (citable) accession number: Q9JIT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.