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Q9JIS8

- S12A4_MOUSE

UniProt

Q9JIS8 - S12A4_MOUSE

Protein

Solute carrier family 12 member 4

Gene

Slc12a4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (13 Dec 2002)
      Previous versions | rss
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    Functioni

    Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl- exit in NaCl absorbing epithelia.

    Enzyme regulationi

    Inhibited by WNK3.By similarity

    GO - Molecular functioni

    1. potassium:chloride symporter activity Source: InterPro

    GO - Biological processi

    1. potassium ion transport Source: UniProtKB-KW

    Keywords - Biological processi

    Ion transport, Potassium transport, Symport, Transport

    Keywords - Ligandi

    Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 12 member 4
    Alternative name(s):
    Electroneutral potassium-chloride cotransporter 1
    Erythroid K-Cl cotransporter 1
    Short name:
    mKCC1
    Gene namesi
    Name:Slc12a4
    Synonyms:Kcc1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1309465. Slc12a4.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10851085Solute carrier family 12 member 4PRO_0000178031Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei47 – 471Phosphoserine1 Publication
    Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi331 – 3311N-linked (GlcNAc...)2 Publications
    Glycosylationi347 – 3471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
    Modified residuei967 – 9671PhosphoserineBy similarity

    Post-translational modificationi

    N-glycosylated.2 Publications

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ9JIS8.
    PRIDEiQ9JIS8.

    PTM databases

    PhosphoSiteiQ9JIS8.

    Expressioni

    Tissue specificityi

    Detected in embryo, adult heart, erythrocytes, brain, kidney, stomach, ovary, testis and liver.

    Developmental stagei

    Expression levels remained constant upon induction of erythroid differentiation of embryonic stem cells. Not detected in reticulocytes, but present during differentiation of erythroleukemia cells to erythroblasts.

    Gene expression databases

    ArrayExpressiQ9JIS8.
    BgeeiQ9JIS8.
    CleanExiMM_SLC12A4.
    GenevestigatoriQ9JIS8.

    Interactioni

    Subunit structurei

    Homomultimer and heteromultimer with other K-Cl cotransporters.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JIS8.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 118118CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini170 – 21445CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini274 – 2752CytoplasmicSequence Analysis
    Topological domaini377 – 41539CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini474 – 49320CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini588 – 62740CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini866 – 1085220CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei119 – 13921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei149 – 16921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei215 – 23521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei253 – 27321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei276 – 29621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei356 – 37621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei416 – 43621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei453 – 47321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei494 – 51421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei567 – 58721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei628 – 64821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei845 – 86521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi160 – 1634Poly-Cys
    Compositional biasi969 – 9724Poly-Glu

    Sequence similaritiesi

    Belongs to the SLC12A transporter family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0531.
    GeneTreeiENSGT00560000076892.
    HOGENOMiHOG000092644.
    HOVERGENiHBG052852.
    KOiK14427.
    OrthoDBiEOG78M01J.
    PhylomeDBiQ9JIS8.
    TreeFamiTF313657.

    Family and domain databases

    InterProiIPR004841. AA-permease/SLC12A_dom.
    IPR000622. K/Cl_cotranspt1.
    IPR018491. K/Cl_cotranspt_1/3.
    IPR000076. KCL_cotranspt.
    IPR004842. Na/K/Cl_cotransptS.
    [Graphical view]
    PfamiPF00324. AA_permease. 2 hits.
    PF03522. KCl_Cotrans_1. 1 hit.
    [Graphical view]
    PRINTSiPR01081. KCLTRNSPORT.
    PR01082. KCLTRNSPORT1.
    TIGRFAMsiTIGR00930. 2a30. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9JIS8-1 [UniParc]FASTAAdd to Basket

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    MPHFTVVPVD GPRRGDYDNL EGLSWVDYGE RAEREDSDGQ GNHRENSPFL     50
    CPLDASRGND YYDRNLALFE EELDIRPKVS SLLGKLVSYT NLTQGAKEHE 100
    EAESGEGGRR RAAKAPSMGT LMGVYLPCLQ NIFGVILFLR LTWMVGTAGV 150
    LQALLIVLIC CCCTLLTAIS MSAIATNGVV PAGGSYFMIS RSLGPEFGGA 200
    VGLCFYLGTT FAAAMYILGA IEILLTYIAP PAAIFYPSGT HDMSSATLNN 250
    MRVYGTIFLT LMTLVVFVGV KYVNKFASLF LACVIISILS IYAGGIKSIF 300
    DPPVFPVCML GNRTLSRDQF DICAKTVVVD NETVATRLWT FFCHSPNLTA 350
    DSCDPYFLLN NVTEIPGIPG AAAGVLQENL WSAYLEKGEV VEKHGLPSTD 400
    TLGLKESLSL YVVADIATSF TVLVGIFFPS VTGIMAGSNR SGDLRDAQKS 450
    IPVGTILAIV TTSLVYFSSV ILFGACIEGV VLRDKYGDGV SRNLVVGTLA 500
    WPSPWVIVVG SFFSTCGAGL QSLTGAPRLL QAIAKDNIIP FLRVFGHGKA 550
    NGEPTWALLL TALIAELGIL IASLDMVAPI LSMFFLMCYL FVNLACAVQT 600
    LLRTPNWRPR FKYYHWTLSF LGMSLCLALM FVSSWYYALV AMLIAGMIYK 650
    YIEYQGAEKE WGDGIRGLSL SAARYALLRL EEGPPHTKNW RPQLLVLLKL 700
    DEDLHVKYPR LLTFASQLKA GKGLTIVGSV IQGSFLESYG EAQAAEQTIK 750
    NMMDIEKVKG FCQVVVASKV REGLAHLIQS CGLGGMRHNS VVLGWPYGWR 800
    QSEDPRAWKT FIDTVRCTTA AHLALLVPKN IAFYPSNHER YLDGHIDVWW 850
    IVHDGGMLML LPFLLRQHKV WKKCRMRIFT VAQMDDNSIQ MKKDLAIFLY 900
    HLRLEAEVEV VEMHNSDISA YTYERTLMME QRSQMLRQMR LTKTERDREA 950
    QLVKDRHSAL RLESLYSDEE EESVAGADKI QMTWTRDKYM AEPWDPSHAP 1000
    DNFRELVHIK PDQSNVRRMH TAVKLNEVIV TRSHDARLVL LNMPGPPKNS 1050
    EGDENYMEFL EVLTEGLERV LLVRGGGREV ITIYS 1085
    Length:1,085
    Mass (Da):120,624
    Last modified:December 13, 2002 - v2
    Checksum:i9094931060972E03
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF047339 mRNA. Translation: AAC32816.1.
    AF121118 mRNA. Translation: AAF02444.1.
    AF191023 Genomic DNA. Translation: AAF91094.1.
    AH009673 Genomic DNA. Translation: AAF91090.1.
    CCDSiCCDS22623.1.
    RefSeqiNP_033221.1. NM_009195.3.
    UniGeneiMm.292447.

    Genome annotation databases

    EnsembliENSMUST00000116429; ENSMUSP00000112130; ENSMUSG00000017765.
    GeneIDi20498.
    KEGGimmu:20498.
    UCSCiuc009ner.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF047339 mRNA. Translation: AAC32816.1 .
    AF121118 mRNA. Translation: AAF02444.1 .
    AF191023 Genomic DNA. Translation: AAF91094.1 .
    AH009673 Genomic DNA. Translation: AAF91090.1 .
    CCDSi CCDS22623.1.
    RefSeqi NP_033221.1. NM_009195.3.
    UniGenei Mm.292447.

    3D structure databases

    ProteinModelPortali Q9JIS8.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9JIS8.

    Proteomic databases

    PaxDbi Q9JIS8.
    PRIDEi Q9JIS8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000116429 ; ENSMUSP00000112130 ; ENSMUSG00000017765 .
    GeneIDi 20498.
    KEGGi mmu:20498.
    UCSCi uc009ner.2. mouse.

    Organism-specific databases

    CTDi 6560.
    MGIi MGI:1309465. Slc12a4.

    Phylogenomic databases

    eggNOGi COG0531.
    GeneTreei ENSGT00560000076892.
    HOGENOMi HOG000092644.
    HOVERGENi HBG052852.
    KOi K14427.
    OrthoDBi EOG78M01J.
    PhylomeDBi Q9JIS8.
    TreeFami TF313657.

    Miscellaneous databases

    ChiTaRSi SLC12A4. mouse.
    NextBioi 298659.
    PROi Q9JIS8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JIS8.
    Bgeei Q9JIS8.
    CleanExi MM_SLC12A4.
    Genevestigatori Q9JIS8.

    Family and domain databases

    InterProi IPR004841. AA-permease/SLC12A_dom.
    IPR000622. K/Cl_cotranspt1.
    IPR018491. K/Cl_cotranspt_1/3.
    IPR000076. KCL_cotranspt.
    IPR004842. Na/K/Cl_cotransptS.
    [Graphical view ]
    Pfami PF00324. AA_permease. 2 hits.
    PF03522. KCl_Cotrans_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01081. KCLTRNSPORT.
    PR01082. KCLTRNSPORT1.
    TIGRFAMsi TIGR00930. 2a30. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular identification and expression of erythroid K:Cl cotransporter in human and mouse erythroleukemic cells."
      Pellegrino C.M., Rybicki A.C., Musto S., Nagel R.L., Schwartz R.S.
      Blood Cells Mol. Dis. 24:31-40(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: DBA.
      Tissue: Erythroleukemia.
    2. "Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation."
      Su W., Shmukler B.E., Chernova M.N., Stuart-Tilley A.K., de Franceschi L., Brugnara C., Alper S.L.
      Am. J. Physiol. 277:C899-C912(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Strain: CD-1.
      Tissue: Brain and Spleen.
    3. "Structure and genetic polymorphism of the mouse KCC1 gene."
      Shmukler B.E., Brugnara C., Alper S.L.
      Biochim. Biophys. Acta 1492:353-361(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    4. "A dominant negative mutant of the KCC1 K-Cl cotransporter: both N- and C-terminal cytoplasmic domains are required for K-Cl cotransport activity."
      Casula S., Shmukler B.E., Wilhelm S., Stuart-Tilley A.K., Su W., Chernova M.N., Brugnara C., Alper S.L.
      J. Biol. Chem. 276:41870-41878(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
      Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
      Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-331.
      Tissue: Myoblast.
    8. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-331.

    Entry informationi

    Entry nameiS12A4_MOUSE
    AccessioniPrimary (citable) accession number: Q9JIS8
    Secondary accession number(s): O55069, Q9ET57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 13, 2002
    Last sequence update: December 13, 2002
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Activated by N-ethylmaleimide (NEM). Inhibited by DIOA.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3