Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Solute carrier family 12 member 4

Gene

Slc12a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl- exit in NaCl absorbing epithelia.

Enzyme regulationi

Inhibited by WNK3.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Symport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-MMU-426117. Cation-coupled Chloride cotransporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 12 member 4
Alternative name(s):
Electroneutral potassium-chloride cotransporter 1
Erythroid K-Cl cotransporter 1
Short name:
mKCC1
Gene namesi
Name:Slc12a4
Synonyms:Kcc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1309465. Slc12a4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 118CytoplasmicSequence analysisAdd BLAST118
Transmembranei119 – 139HelicalSequence analysisAdd BLAST21
Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
Topological domaini170 – 214CytoplasmicSequence analysisAdd BLAST45
Transmembranei215 – 235HelicalSequence analysisAdd BLAST21
Transmembranei253 – 273HelicalSequence analysisAdd BLAST21
Topological domaini274 – 275CytoplasmicSequence analysis2
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Transmembranei356 – 376HelicalSequence analysisAdd BLAST21
Topological domaini377 – 415CytoplasmicSequence analysisAdd BLAST39
Transmembranei416 – 436HelicalSequence analysisAdd BLAST21
Transmembranei453 – 473HelicalSequence analysisAdd BLAST21
Topological domaini474 – 493CytoplasmicSequence analysisAdd BLAST20
Transmembranei494 – 514HelicalSequence analysisAdd BLAST21
Transmembranei567 – 587HelicalSequence analysisAdd BLAST21
Topological domaini588 – 627CytoplasmicSequence analysisAdd BLAST40
Transmembranei628 – 648HelicalSequence analysisAdd BLAST21
Transmembranei845 – 865HelicalSequence analysisAdd BLAST21
Topological domaini866 – 1085CytoplasmicSequence analysisAdd BLAST220

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001780311 – 1085Solute carrier family 12 member 4Add BLAST1085

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineCombined sources1
Modified residuei47PhosphoserineCombined sources1
Modified residuei81PhosphoserineBy similarity1
Modified residuei88PhosphoserineBy similarity1
Glycosylationi312N-linked (GlcNAc...)Sequence analysis1
Glycosylationi331N-linked (GlcNAc...)2 Publications1
Glycosylationi347N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)Sequence analysis1
Modified residuei967PhosphoserineCombined sources1
Modified residuei983PhosphothreonineBy similarity1

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9JIS8.
MaxQBiQ9JIS8.
PaxDbiQ9JIS8.
PRIDEiQ9JIS8.

PTM databases

iPTMnetiQ9JIS8.
PhosphoSitePlusiQ9JIS8.

Expressioni

Tissue specificityi

Detected in embryo, adult heart, erythrocytes, brain, kidney, stomach, ovary, testis and liver.

Developmental stagei

Expression levels remained constant upon induction of erythroid differentiation of embryonic stem cells. Not detected in reticulocytes, but present during differentiation of erythroleukemia cells to erythroblasts.

Gene expression databases

BgeeiENSMUSG00000017765.
CleanExiMM_SLC12A4.
ExpressionAtlasiQ9JIS8. baseline and differential.
GenevisibleiQ9JIS8. MM.

Interactioni

Subunit structurei

Homomultimer and heteromultimer with other K-Cl cotransporters.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9JIS8. 4 interactors.
STRINGi10090.ENSMUSP00000034370.

Structurei

3D structure databases

ProteinModelPortaliQ9JIS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi160 – 163Poly-Cys4
Compositional biasi969 – 972Poly-Glu4

Sequence similaritiesi

Belongs to the SLC12A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2082. Eukaryota.
COG0531. LUCA.
GeneTreeiENSGT00760000119053.
HOGENOMiHOG000092644.
HOVERGENiHBG052852.
InParanoidiQ9JIS8.
KOiK14427.
PhylomeDBiQ9JIS8.
TreeFamiTF313657.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR000622. KCC1.
IPR000076. KCL_cotranspt.
IPR018491. SLC12_C.
IPR004842. SLC12A_fam.
[Graphical view]
PANTHERiPTHR11827:SF46. PTHR11827:SF46. 4 hits.
PfamiPF00324. AA_permease. 2 hits.
PF03522. SLC12. 2 hits.
[Graphical view]
PRINTSiPR01081. KCLTRNSPORT.
PR01082. KCLTRNSPORT1.
TIGRFAMsiTIGR00930. 2a30. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JIS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHFTVVPVD GPRRGDYDNL EGLSWVDYGE RAEREDSDGQ GNHRENSPFL
60 70 80 90 100
CPLDASRGND YYDRNLALFE EELDIRPKVS SLLGKLVSYT NLTQGAKEHE
110 120 130 140 150
EAESGEGGRR RAAKAPSMGT LMGVYLPCLQ NIFGVILFLR LTWMVGTAGV
160 170 180 190 200
LQALLIVLIC CCCTLLTAIS MSAIATNGVV PAGGSYFMIS RSLGPEFGGA
210 220 230 240 250
VGLCFYLGTT FAAAMYILGA IEILLTYIAP PAAIFYPSGT HDMSSATLNN
260 270 280 290 300
MRVYGTIFLT LMTLVVFVGV KYVNKFASLF LACVIISILS IYAGGIKSIF
310 320 330 340 350
DPPVFPVCML GNRTLSRDQF DICAKTVVVD NETVATRLWT FFCHSPNLTA
360 370 380 390 400
DSCDPYFLLN NVTEIPGIPG AAAGVLQENL WSAYLEKGEV VEKHGLPSTD
410 420 430 440 450
TLGLKESLSL YVVADIATSF TVLVGIFFPS VTGIMAGSNR SGDLRDAQKS
460 470 480 490 500
IPVGTILAIV TTSLVYFSSV ILFGACIEGV VLRDKYGDGV SRNLVVGTLA
510 520 530 540 550
WPSPWVIVVG SFFSTCGAGL QSLTGAPRLL QAIAKDNIIP FLRVFGHGKA
560 570 580 590 600
NGEPTWALLL TALIAELGIL IASLDMVAPI LSMFFLMCYL FVNLACAVQT
610 620 630 640 650
LLRTPNWRPR FKYYHWTLSF LGMSLCLALM FVSSWYYALV AMLIAGMIYK
660 670 680 690 700
YIEYQGAEKE WGDGIRGLSL SAARYALLRL EEGPPHTKNW RPQLLVLLKL
710 720 730 740 750
DEDLHVKYPR LLTFASQLKA GKGLTIVGSV IQGSFLESYG EAQAAEQTIK
760 770 780 790 800
NMMDIEKVKG FCQVVVASKV REGLAHLIQS CGLGGMRHNS VVLGWPYGWR
810 820 830 840 850
QSEDPRAWKT FIDTVRCTTA AHLALLVPKN IAFYPSNHER YLDGHIDVWW
860 870 880 890 900
IVHDGGMLML LPFLLRQHKV WKKCRMRIFT VAQMDDNSIQ MKKDLAIFLY
910 920 930 940 950
HLRLEAEVEV VEMHNSDISA YTYERTLMME QRSQMLRQMR LTKTERDREA
960 970 980 990 1000
QLVKDRHSAL RLESLYSDEE EESVAGADKI QMTWTRDKYM AEPWDPSHAP
1010 1020 1030 1040 1050
DNFRELVHIK PDQSNVRRMH TAVKLNEVIV TRSHDARLVL LNMPGPPKNS
1060 1070 1080
EGDENYMEFL EVLTEGLERV LLVRGGGREV ITIYS
Length:1,085
Mass (Da):120,624
Last modified:December 13, 2002 - v2
Checksum:i9094931060972E03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047339 mRNA. Translation: AAC32816.1.
AF121118 mRNA. Translation: AAF02444.1.
AF191023 Genomic DNA. Translation: AAF91094.1.
AH009673 Genomic DNA. Translation: AAF91090.1.
CCDSiCCDS22623.1.
RefSeqiNP_033221.1. NM_009195.3.
UniGeneiMm.292447.

Genome annotation databases

EnsembliENSMUST00000116429; ENSMUSP00000112130; ENSMUSG00000017765.
GeneIDi20498.
KEGGimmu:20498.
UCSCiuc009ner.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047339 mRNA. Translation: AAC32816.1.
AF121118 mRNA. Translation: AAF02444.1.
AF191023 Genomic DNA. Translation: AAF91094.1.
AH009673 Genomic DNA. Translation: AAF91090.1.
CCDSiCCDS22623.1.
RefSeqiNP_033221.1. NM_009195.3.
UniGeneiMm.292447.

3D structure databases

ProteinModelPortaliQ9JIS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JIS8. 4 interactors.
STRINGi10090.ENSMUSP00000034370.

PTM databases

iPTMnetiQ9JIS8.
PhosphoSitePlusiQ9JIS8.

Proteomic databases

EPDiQ9JIS8.
MaxQBiQ9JIS8.
PaxDbiQ9JIS8.
PRIDEiQ9JIS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000116429; ENSMUSP00000112130; ENSMUSG00000017765.
GeneIDi20498.
KEGGimmu:20498.
UCSCiuc009ner.2. mouse.

Organism-specific databases

CTDi6560.
MGIiMGI:1309465. Slc12a4.

Phylogenomic databases

eggNOGiKOG2082. Eukaryota.
COG0531. LUCA.
GeneTreeiENSGT00760000119053.
HOGENOMiHOG000092644.
HOVERGENiHBG052852.
InParanoidiQ9JIS8.
KOiK14427.
PhylomeDBiQ9JIS8.
TreeFamiTF313657.

Enzyme and pathway databases

ReactomeiR-MMU-426117. Cation-coupled Chloride cotransporters.

Miscellaneous databases

ChiTaRSiSlc12a4. mouse.
PROiQ9JIS8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017765.
CleanExiMM_SLC12A4.
ExpressionAtlasiQ9JIS8. baseline and differential.
GenevisibleiQ9JIS8. MM.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR000622. KCC1.
IPR000076. KCL_cotranspt.
IPR018491. SLC12_C.
IPR004842. SLC12A_fam.
[Graphical view]
PANTHERiPTHR11827:SF46. PTHR11827:SF46. 4 hits.
PfamiPF00324. AA_permease. 2 hits.
PF03522. SLC12. 2 hits.
[Graphical view]
PRINTSiPR01081. KCLTRNSPORT.
PR01082. KCLTRNSPORT1.
TIGRFAMsiTIGR00930. 2a30. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS12A4_MOUSE
AccessioniPrimary (citable) accession number: Q9JIS8
Secondary accession number(s): O55069, Q9ET57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: December 13, 2002
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Activated by N-ethylmaleimide (NEM). Inhibited by DIOA.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.