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Q9JIS8

- S12A4_MOUSE

UniProt

Q9JIS8 - S12A4_MOUSE

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Protein

Solute carrier family 12 member 4

Gene

Slc12a4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl- exit in NaCl absorbing epithelia.

Enzyme regulationi

Inhibited by WNK3.By similarity

GO - Molecular functioni

  1. potassium:chloride symporter activity Source: InterPro

GO - Biological processi

  1. potassium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Symport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 12 member 4
Alternative name(s):
Electroneutral potassium-chloride cotransporter 1
Erythroid K-Cl cotransporter 1
Short name:
mKCC1
Gene namesi
Name:Slc12a4
Synonyms:Kcc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1309465. Slc12a4.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. lysosomal membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10851085Solute carrier family 12 member 4PRO_0000178031Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei47 – 471Phosphoserine1 Publication
Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)2 Publications
Glycosylationi347 – 3471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
Modified residuei967 – 9671PhosphoserineBy similarity

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9JIS8.
PaxDbiQ9JIS8.
PRIDEiQ9JIS8.

PTM databases

PhosphoSiteiQ9JIS8.

Expressioni

Tissue specificityi

Detected in embryo, adult heart, erythrocytes, brain, kidney, stomach, ovary, testis and liver.

Developmental stagei

Expression levels remained constant upon induction of erythroid differentiation of embryonic stem cells. Not detected in reticulocytes, but present during differentiation of erythroleukemia cells to erythroblasts.

Gene expression databases

BgeeiQ9JIS8.
CleanExiMM_SLC12A4.
ExpressionAtlasiQ9JIS8. baseline and differential.
GenevestigatoriQ9JIS8.

Interactioni

Subunit structurei

Homomultimer and heteromultimer with other K-Cl cotransporters.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9JIS8.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 118118CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini170 – 21445CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini274 – 2752CytoplasmicSequence Analysis
Topological domaini377 – 41539CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini474 – 49320CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini588 – 62740CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini866 – 1085220CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei119 – 13921HelicalSequence AnalysisAdd
BLAST
Transmembranei149 – 16921HelicalSequence AnalysisAdd
BLAST
Transmembranei215 – 23521HelicalSequence AnalysisAdd
BLAST
Transmembranei253 – 27321HelicalSequence AnalysisAdd
BLAST
Transmembranei276 – 29621HelicalSequence AnalysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence AnalysisAdd
BLAST
Transmembranei416 – 43621HelicalSequence AnalysisAdd
BLAST
Transmembranei453 – 47321HelicalSequence AnalysisAdd
BLAST
Transmembranei494 – 51421HelicalSequence AnalysisAdd
BLAST
Transmembranei567 – 58721HelicalSequence AnalysisAdd
BLAST
Transmembranei628 – 64821HelicalSequence AnalysisAdd
BLAST
Transmembranei845 – 86521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi160 – 1634Poly-Cys
Compositional biasi969 – 9724Poly-Glu

Sequence similaritiesi

Belongs to the SLC12A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0531.
GeneTreeiENSGT00760000119053.
HOGENOMiHOG000092644.
HOVERGENiHBG052852.
InParanoidiQ9JIS8.
KOiK14427.
OrthoDBiEOG78M01J.
PhylomeDBiQ9JIS8.
TreeFamiTF313657.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR000622. K/Cl_cotranspt1.
IPR018491. K/Cl_cotranspt_1/3.
IPR000076. KCL_cotranspt.
IPR004842. Na/K/Cl_cotransptS.
[Graphical view]
PfamiPF00324. AA_permease. 2 hits.
PF03522. KCl_Cotrans_1. 1 hit.
[Graphical view]
PRINTSiPR01081. KCLTRNSPORT.
PR01082. KCLTRNSPORT1.
TIGRFAMsiTIGR00930. 2a30. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JIS8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPHFTVVPVD GPRRGDYDNL EGLSWVDYGE RAEREDSDGQ GNHRENSPFL
60 70 80 90 100
CPLDASRGND YYDRNLALFE EELDIRPKVS SLLGKLVSYT NLTQGAKEHE
110 120 130 140 150
EAESGEGGRR RAAKAPSMGT LMGVYLPCLQ NIFGVILFLR LTWMVGTAGV
160 170 180 190 200
LQALLIVLIC CCCTLLTAIS MSAIATNGVV PAGGSYFMIS RSLGPEFGGA
210 220 230 240 250
VGLCFYLGTT FAAAMYILGA IEILLTYIAP PAAIFYPSGT HDMSSATLNN
260 270 280 290 300
MRVYGTIFLT LMTLVVFVGV KYVNKFASLF LACVIISILS IYAGGIKSIF
310 320 330 340 350
DPPVFPVCML GNRTLSRDQF DICAKTVVVD NETVATRLWT FFCHSPNLTA
360 370 380 390 400
DSCDPYFLLN NVTEIPGIPG AAAGVLQENL WSAYLEKGEV VEKHGLPSTD
410 420 430 440 450
TLGLKESLSL YVVADIATSF TVLVGIFFPS VTGIMAGSNR SGDLRDAQKS
460 470 480 490 500
IPVGTILAIV TTSLVYFSSV ILFGACIEGV VLRDKYGDGV SRNLVVGTLA
510 520 530 540 550
WPSPWVIVVG SFFSTCGAGL QSLTGAPRLL QAIAKDNIIP FLRVFGHGKA
560 570 580 590 600
NGEPTWALLL TALIAELGIL IASLDMVAPI LSMFFLMCYL FVNLACAVQT
610 620 630 640 650
LLRTPNWRPR FKYYHWTLSF LGMSLCLALM FVSSWYYALV AMLIAGMIYK
660 670 680 690 700
YIEYQGAEKE WGDGIRGLSL SAARYALLRL EEGPPHTKNW RPQLLVLLKL
710 720 730 740 750
DEDLHVKYPR LLTFASQLKA GKGLTIVGSV IQGSFLESYG EAQAAEQTIK
760 770 780 790 800
NMMDIEKVKG FCQVVVASKV REGLAHLIQS CGLGGMRHNS VVLGWPYGWR
810 820 830 840 850
QSEDPRAWKT FIDTVRCTTA AHLALLVPKN IAFYPSNHER YLDGHIDVWW
860 870 880 890 900
IVHDGGMLML LPFLLRQHKV WKKCRMRIFT VAQMDDNSIQ MKKDLAIFLY
910 920 930 940 950
HLRLEAEVEV VEMHNSDISA YTYERTLMME QRSQMLRQMR LTKTERDREA
960 970 980 990 1000
QLVKDRHSAL RLESLYSDEE EESVAGADKI QMTWTRDKYM AEPWDPSHAP
1010 1020 1030 1040 1050
DNFRELVHIK PDQSNVRRMH TAVKLNEVIV TRSHDARLVL LNMPGPPKNS
1060 1070 1080
EGDENYMEFL EVLTEGLERV LLVRGGGREV ITIYS
Length:1,085
Mass (Da):120,624
Last modified:December 13, 2002 - v2
Checksum:i9094931060972E03
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF047339 mRNA. Translation: AAC32816.1.
AF121118 mRNA. Translation: AAF02444.1.
AF191023 Genomic DNA. Translation: AAF91094.1.
AH009673 Genomic DNA. Translation: AAF91090.1.
CCDSiCCDS22623.1.
RefSeqiNP_033221.1. NM_009195.3.
UniGeneiMm.292447.

Genome annotation databases

EnsembliENSMUST00000116429; ENSMUSP00000112130; ENSMUSG00000017765.
GeneIDi20498.
KEGGimmu:20498.
UCSCiuc009ner.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF047339 mRNA. Translation: AAC32816.1 .
AF121118 mRNA. Translation: AAF02444.1 .
AF191023 Genomic DNA. Translation: AAF91094.1 .
AH009673 Genomic DNA. Translation: AAF91090.1 .
CCDSi CCDS22623.1.
RefSeqi NP_033221.1. NM_009195.3.
UniGenei Mm.292447.

3D structure databases

ProteinModelPortali Q9JIS8.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q9JIS8.

Proteomic databases

MaxQBi Q9JIS8.
PaxDbi Q9JIS8.
PRIDEi Q9JIS8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000116429 ; ENSMUSP00000112130 ; ENSMUSG00000017765 .
GeneIDi 20498.
KEGGi mmu:20498.
UCSCi uc009ner.2. mouse.

Organism-specific databases

CTDi 6560.
MGIi MGI:1309465. Slc12a4.

Phylogenomic databases

eggNOGi COG0531.
GeneTreei ENSGT00760000119053.
HOGENOMi HOG000092644.
HOVERGENi HBG052852.
InParanoidi Q9JIS8.
KOi K14427.
OrthoDBi EOG78M01J.
PhylomeDBi Q9JIS8.
TreeFami TF313657.

Miscellaneous databases

ChiTaRSi SLC12A4. mouse.
NextBioi 298659.
PROi Q9JIS8.
SOURCEi Search...

Gene expression databases

Bgeei Q9JIS8.
CleanExi MM_SLC12A4.
ExpressionAtlasi Q9JIS8. baseline and differential.
Genevestigatori Q9JIS8.

Family and domain databases

InterProi IPR004841. AA-permease/SLC12A_dom.
IPR000622. K/Cl_cotranspt1.
IPR018491. K/Cl_cotranspt_1/3.
IPR000076. KCL_cotranspt.
IPR004842. Na/K/Cl_cotransptS.
[Graphical view ]
Pfami PF00324. AA_permease. 2 hits.
PF03522. KCl_Cotrans_1. 1 hit.
[Graphical view ]
PRINTSi PR01081. KCLTRNSPORT.
PR01082. KCLTRNSPORT1.
TIGRFAMsi TIGR00930. 2a30. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular identification and expression of erythroid K:Cl cotransporter in human and mouse erythroleukemic cells."
    Pellegrino C.M., Rybicki A.C., Musto S., Nagel R.L., Schwartz R.S.
    Blood Cells Mol. Dis. 24:31-40(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: DBA.
    Tissue: Erythroleukemia.
  2. "Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation."
    Su W., Shmukler B.E., Chernova M.N., Stuart-Tilley A.K., de Franceschi L., Brugnara C., Alper S.L.
    Am. J. Physiol. 277:C899-C912(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: CD-1.
    Tissue: Brain and Spleen.
  3. "Structure and genetic polymorphism of the mouse KCC1 gene."
    Shmukler B.E., Brugnara C., Alper S.L.
    Biochim. Biophys. Acta 1492:353-361(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  4. "A dominant negative mutant of the KCC1 K-Cl cotransporter: both N- and C-terminal cytoplasmic domains are required for K-Cl cotransport activity."
    Casula S., Shmukler B.E., Wilhelm S., Stuart-Tilley A.K., Su W., Chernova M.N., Brugnara C., Alper S.L.
    J. Biol. Chem. 276:41870-41878(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-331.
    Tissue: Myoblast.
  8. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-331.

Entry informationi

Entry nameiS12A4_MOUSE
AccessioniPrimary (citable) accession number: Q9JIS8
Secondary accession number(s): O55069, Q9ET57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: December 13, 2002
Last modified: October 29, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Activated by N-ethylmaleimide (NEM). Inhibited by DIOA.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3