Solute carrier family 12 member 4 - Mus musculus (Mouse)

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Q9JIS8 (S12A4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 91. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Solute carrier family 12 member 4

Alternative name(s):
Electroneutral potassium-chloride cotransporter 1
Erythroid K-Cl cotransporter 1
Short name=mKCC1

Gene names

Name:	Slc12a4
Synonyms:	Kcc1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1085 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl^- exit in NaCl absorbing epithelia.
Enzyme regulation	Inhibited by WNK3 By similarity.
Subunit structure	Homomultimer and heteromultimer with other K-Cl cotransporters. Ref.4
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Detected in embryo, adult heart, erythrocytes, brain, kidney, stomach, ovary, testis and liver.
Developmental stage	Expression levels remained constant upon induction of erythroid differentiation of embryonic stem cells. Not detected in reticulocytes, but present during differentiation of erythroleukemia cells to erythroblasts.
Post-translational modification	N-glycosylated.
Miscellaneous	Activated by N-ethylmaleimide (NEM). Inhibited by DIOA.
Sequence similarities	Belongs to the SLC12A transporter family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Symport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	potassium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	potassium:chloride symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1085

1085

Solute carrier family 12 member 4

PRO_0000178031

Regions

Topological domain

1 – 118

118

Cytoplasmic Potential

Transmembrane

119 – 139

Helical; Potential

Transmembrane

149 – 169

Helical; Potential

Topological domain

170 – 214

Cytoplasmic Potential

Transmembrane

215 – 235

Helical; Potential

Transmembrane

253 – 273

Helical; Potential

Topological domain

274 – 275

Cytoplasmic Potential

Transmembrane

276 – 296

Helical; Potential

Transmembrane

356 – 376

Helical; Potential

Topological domain

377 – 415

Cytoplasmic Potential

Transmembrane

416 – 436

Helical; Potential

Transmembrane

453 – 473

Helical; Potential

Topological domain

474 – 493

Cytoplasmic Potential

Transmembrane

494 – 514

Helical; Potential

Transmembrane

567 – 587

Helical; Potential

Topological domain

588 – 627

Cytoplasmic Potential

Transmembrane

628 – 648

Helical; Potential

Transmembrane

845 – 865

Helical; Potential

Topological domain

866 – 1085

220

Cytoplasmic Potential

Compositional bias

160 – 163

Poly-Cys

Compositional bias

969 – 972

Poly-Glu

Amino acid modifications

Modified residue

Phosphoserine Ref.6

Modified residue

967

Phosphoserine Ref.5

Modified residue

983

Phosphothreonine Ref.6

Glycosylation

312

N-linked (GlcNAc...) Potential

Glycosylation

331

N-linked (GlcNAc...) Ref.7 Ref.8

Glycosylation

347

N-linked (GlcNAc...) Potential

Glycosylation

439

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q9JIS8 [UniParc].

Last modified December 13, 2002. Version 2.
Checksum: 9094931060972E03
Show »

FASTA

1,085

120,624

        10         20         30         40         50         60 
MPHFTVVPVD GPRRGDYDNL EGLSWVDYGE RAEREDSDGQ GNHRENSPFL CPLDASRGND 

        70         80         90        100        110        120 
YYDRNLALFE EELDIRPKVS SLLGKLVSYT NLTQGAKEHE EAESGEGGRR RAAKAPSMGT 

       130        140        150        160        170        180 
LMGVYLPCLQ NIFGVILFLR LTWMVGTAGV LQALLIVLIC CCCTLLTAIS MSAIATNGVV 

       190        200        210        220        230        240 
PAGGSYFMIS RSLGPEFGGA VGLCFYLGTT FAAAMYILGA IEILLTYIAP PAAIFYPSGT 

       250        260        270        280        290        300 
HDMSSATLNN MRVYGTIFLT LMTLVVFVGV KYVNKFASLF LACVIISILS IYAGGIKSIF 

       310        320        330        340        350        360 
DPPVFPVCML GNRTLSRDQF DICAKTVVVD NETVATRLWT FFCHSPNLTA DSCDPYFLLN 

       370        380        390        400        410        420 
NVTEIPGIPG AAAGVLQENL WSAYLEKGEV VEKHGLPSTD TLGLKESLSL YVVADIATSF 

       430        440        450        460        470        480 
TVLVGIFFPS VTGIMAGSNR SGDLRDAQKS IPVGTILAIV TTSLVYFSSV ILFGACIEGV 

       490        500        510        520        530        540 
VLRDKYGDGV SRNLVVGTLA WPSPWVIVVG SFFSTCGAGL QSLTGAPRLL QAIAKDNIIP 

       550        560        570        580        590        600 
FLRVFGHGKA NGEPTWALLL TALIAELGIL IASLDMVAPI LSMFFLMCYL FVNLACAVQT 

       610        620        630        640        650        660 
LLRTPNWRPR FKYYHWTLSF LGMSLCLALM FVSSWYYALV AMLIAGMIYK YIEYQGAEKE 

       670        680        690        700        710        720 
WGDGIRGLSL SAARYALLRL EEGPPHTKNW RPQLLVLLKL DEDLHVKYPR LLTFASQLKA 

       730        740        750        760        770        780 
GKGLTIVGSV IQGSFLESYG EAQAAEQTIK NMMDIEKVKG FCQVVVASKV REGLAHLIQS 

       790        800        810        820        830        840 
CGLGGMRHNS VVLGWPYGWR QSEDPRAWKT FIDTVRCTTA AHLALLVPKN IAFYPSNHER 

       850        860        870        880        890        900 
YLDGHIDVWW IVHDGGMLML LPFLLRQHKV WKKCRMRIFT VAQMDDNSIQ MKKDLAIFLY 

       910        920        930        940        950        960 
HLRLEAEVEV VEMHNSDISA YTYERTLMME QRSQMLRQMR LTKTERDREA QLVKDRHSAL 

       970        980        990       1000       1010       1020 
RLESLYSDEE EESVAGADKI QMTWTRDKYM AEPWDPSHAP DNFRELVHIK PDQSNVRRMH 

      1030       1040       1050       1060       1070       1080 
TAVKLNEVIV TRSHDARLVL LNMPGPPKNS EGDENYMEFL EVLTEGLERV LLVRGGGREV 


ITIYS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular identification and expression of erythroid K:Cl cotransporter in human and mouse erythroleukemic cells." Pellegrino C.M., Rybicki A.C., Musto S., Nagel R.L., Schwartz R.S. Blood Cells Mol. Dis. 24:31-40(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DBA. Tissue: Erythroleukemia.
[2]	"Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation." Su W., Shmukler B.E., Chernova M.N., Stuart-Tilley A.K., de Franceschi L., Brugnara C., Alper S.L. Am. J. Physiol. 277:C899-C912(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: CD-1. Tissue: Brain and Spleen.
[3]	"Structure and genetic polymorphism of the mouse KCC1 gene." Shmukler B.E., Brugnara C., Alper S.L. Biochim. Biophys. Acta 1492:353-361(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"A dominant negative mutant of the KCC1 K-Cl cotransporter: both N- and C-terminal cytoplasmic domains are required for K-Cl cotransport activity." Casula S., Shmukler B.E., Wilhelm S., Stuart-Tilley A.K., Su W., Chernova M.N., Brugnara C., Alper S.L. J. Biol. Chem. 276:41870-41878(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-967, MASS SPECTROMETRY. Tissue: Liver.
[6]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47 AND THR-983, MASS SPECTROMETRY. Tissue: Macrophage.
[7]	"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-331, MASS SPECTROMETRY. Tissue: Myoblast.
[8]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-331, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF047339 mRNA. Translation: AAC32816.1. AF121118 mRNA. Translation: AAF02444.1. AF191023 Genomic DNA. Translation: AAF91094.1. AH009673 Genomic DNA. Translation: AAF91090.1.
IPI	IPI00115231.
RefSeq	NP_033221.1. NM_009195.3.
UniGene	Mm.292447.
3D structure databases
ProteinModelPortal	Q9JIS8.
SMR	Q9JIS8. Positions 411-595.
ModBase	Search...
PTM databases
PhosphoSite	Q9JIS8.
Proteomic databases
PaxDb	Q9JIS8.
PRIDE	Q9JIS8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000116429; ENSMUSP00000112130; ENSMUSG00000017765.
GeneID	20498.
KEGG	mmu:20498.
Organism-specific databases
CTD	6560.
MGI	MGI:1309465. Slc12a4.
Phylogenomic databases
eggNOG	COG0531.
GeneTree	ENSGT00560000076892.
HOGENOM	HOG000092644.
HOVERGEN	HBG052852.
KO	K14427.
OrthoDB	EOG476JZG.
Gene expression databases
ArrayExpress	Q9JIS8.
Bgee	Q9JIS8.
CleanEx	MM_SLC12A4.
Genevestigator	Q9JIS8.
GermOnline	ENSMUSG00000017765. Mus musculus.
Family and domain databases
InterPro	IPR004841. AA-permease_dom. IPR000622. K/Cl_cotranspt1. IPR018491. K/Cl_cotranspt_1/3. IPR000076. KCL_cotranspt. IPR004842. Na/K/Cl_cotransptS. [Graphical view]
Pfam	PF00324. AA_permease. 2 hits. PF03522. KCl_Cotrans_1. 1 hit. [Graphical view]
PRINTS	PR01081. KCLTRNSPORT. PR01082. KCLTRNSPORT1.
TIGRFAMs	TIGR00930. 2a30. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	SLC12A4. mouse.
NextBio	298659.
SOURCE	Search...

Entry information

Entry name

S12A4_MOUSE

Accession

Primary (citable) accession number: Q9JIS8
Secondary accession number(s): O55069, Q9ET57

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 13, 2002
Last sequence update:	December 13, 2002
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents