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Protein

Voltage-dependent L-type calcium channel subunit alpha-1F

Gene

Cacna1f

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1F gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA) (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei330Calcium ion selectivity and permeabilityBy similarity1
Sitei711Calcium ion selectivity and permeabilityBy similarity1
Sitei1091Calcium ion selectivity and permeabilityBy similarity1
Sitei1388Calcium ion selectivity and permeabilityBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi1475 – 1486By similarityAdd BLAST12

GO - Molecular functioni

  • high voltage-gated calcium channel activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • voltage-gated calcium channel activity Source: MGI

GO - Biological processi

  • axonogenesis Source: MGI
  • cellular calcium ion homeostasis Source: MGI
  • dendrite morphogenesis Source: MGI
  • detection of light stimulus involved in visual perception Source: MGI
  • membrane depolarization during action potential Source: GO_Central
  • regulation of T cell receptor signaling pathway Source: InterPro
  • retina development in camera-type eye Source: MGI
  • T cell homeostasis Source: InterPro
  • visual perception Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Sensory transduction, Transport, Vision

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-419037. NCAM1 interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent L-type calcium channel subunit alpha-1F
Alternative name(s):
Voltage-gated calcium channel subunit alpha Cav1.4
Gene namesi
Name:Cacna1f
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1859639. Cacna1f.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 92CytoplasmicSequence analysisAdd BLAST92
Transmembranei93 – 111Helical; Name=S1 of repeat ISequence analysisAdd BLAST19
Topological domaini112 – 129ExtracellularSequence analysisAdd BLAST18
Transmembranei130 – 149Helical; Name=S2 of repeat ISequence analysisAdd BLAST20
Topological domaini150 – 161CytoplasmicSequence analysisAdd BLAST12
Transmembranei162 – 180Helical; Name=S3 of repeat ISequence analysisAdd BLAST19
Topological domaini181 – 201ExtracellularSequence analysisAdd BLAST21
Transmembranei202 – 220Helical; Name=S4 of repeat ISequence analysisAdd BLAST19
Topological domaini221 – 239CytoplasmicSequence analysisAdd BLAST19
Transmembranei240 – 259Helical; Name=S5 of repeat ISequence analysisAdd BLAST20
Topological domaini260 – 347ExtracellularSequence analysisAdd BLAST88
Transmembranei348 – 372Helical; Name=S6 of repeat ISequence analysisAdd BLAST25
Topological domaini373 – 529CytoplasmicSequence analysisAdd BLAST157
Transmembranei530 – 549Helical; Name=S1 of repeat IISequence analysisAdd BLAST20
Topological domaini550 – 564ExtracellularSequence analysisAdd BLAST15
Transmembranei565 – 583Helical; Name=S2 of repeat IISequence analysisAdd BLAST19
Topological domaini584 – 591CytoplasmicSequence analysis8
Transmembranei592 – 610Helical; Name=S3 of repeat IISequence analysisAdd BLAST19
Topological domaini611 – 620ExtracellularSequence analysis10
Transmembranei621 – 639Helical; Name=S4 of repeat IISequence analysisAdd BLAST19
Topological domaini640 – 658CytoplasmicSequence analysisAdd BLAST19
Transmembranei659 – 679Helical; Name=S5 of repeat IISequence analysisAdd BLAST21
Topological domaini680 – 733ExtracellularSequence analysisAdd BLAST54
Transmembranei734 – 758Helical; Name=S6 of repeat IISequence analysisAdd BLAST25
Topological domaini759 – 876CytoplasmicSequence analysisAdd BLAST118
Transmembranei877 – 895Helical; Name=S1 of repeat IIISequence analysisAdd BLAST19
Topological domaini896 – 911ExtracellularSequence analysisAdd BLAST16
Transmembranei912 – 931Helical; Name=S2 of repeat IIISequence analysisAdd BLAST20
Topological domaini932 – 943CytoplasmicSequence analysisAdd BLAST12
Transmembranei944 – 962Helical; Name=S3 of repeat IIISequence analysisAdd BLAST19
Topological domaini963 – 968ExtracellularSequence analysis6
Transmembranei969 – 988Helical; Name=S4 of repeat IIISequence analysisAdd BLAST20
Topological domaini989 – 1007CytoplasmicSequence analysisAdd BLAST19
Transmembranei1008 – 1027Helical; Name=S5 of repeat IIISequence analysisAdd BLAST20
Topological domaini1028 – 1117ExtracellularSequence analysisAdd BLAST90
Transmembranei1118 – 1138Helical; Name=S6 of repeat IIISequence analysisAdd BLAST21
Topological domaini1139 – 1195CytoplasmicSequence analysisAdd BLAST57
Transmembranei1196 – 1214Helical; Name=S1 of repeat IVSequence analysisAdd BLAST19
Topological domaini1215 – 1229ExtracellularSequence analysisAdd BLAST15
Transmembranei1230 – 1249Helical; Name=S2 of repeat IVSequence analysisAdd BLAST20
Topological domaini1250 – 1256CytoplasmicSequence analysis7
Transmembranei1257 – 1278Helical; Name=S3 of repeat IVSequence analysisAdd BLAST22
Topological domaini1279 – 1295ExtracellularSequence analysisAdd BLAST17
Transmembranei1296 – 1315Helical; Name=S4 of repeat IVSequence analysisAdd BLAST20
Topological domaini1316 – 1334CytoplasmicSequence analysisAdd BLAST19
Transmembranei1335 – 1354Helical; Name=S5 of repeat IVSequence analysisAdd BLAST20
Topological domaini1355 – 1421ExtracellularSequence analysisAdd BLAST67
Transmembranei1422 – 1446Helical; Name=S6 of repeat IVSequence analysisAdd BLAST25
Topological domaini1447 – 1982CytoplasmicSequence analysisAdd BLAST536

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176791.
GuidetoPHARMACOLOGYi531.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000539511 – 1985Voltage-dependent L-type calcium channel subunit alpha-1FAdd BLAST1985

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9JIS7.
PaxDbiQ9JIS7.
PRIDEiQ9JIS7.

PTM databases

iPTMnetiQ9JIS7.
PhosphoSitePlusiQ9JIS7.

Expressioni

Tissue specificityi

Expressed in the inner and outer nuclear layers and the genglion cell layer of the retina.1 Publication

Interactioni

Subunit structurei

Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity (By similarity). Interacts (via IQ domain) with CABP4; in a calcium independent manner.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-61285N.
MINTiMINT-4998297.
STRINGi10090.ENSMUSP00000111391.

Structurei

3D structure databases

ProteinModelPortaliQ9JIS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati79 – 375IAdd BLAST297
Repeati515 – 761IIAdd BLAST247
Repeati858 – 1140IIIAdd BLAST283
Repeati1182 – 1449IVAdd BLAST268

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni395 – 412Binding to the beta subunitBy similarityAdd BLAST18
Regioni1065 – 1155Dihydropyridine bindingBy similarityAdd BLAST91
Regioni1402 – 1468Dihydropyridine bindingBy similarityAdd BLAST67
Regioni1414 – 1457Phenylalkylamine bindingBy similarityAdd BLAST44

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi659 – 665Poly-Leu7
Compositional biasi811 – 829Poly-GluAdd BLAST19
Compositional biasi1126 – 1129Poly-Ile4
Compositional biasi1645 – 1652Poly-Glu8
Compositional biasi1657 – 1664Poly-Glu8

Domaini

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2301. Eukaryota.
ENOG410XNP6. LUCA.
HOGENOMiHOG000231529.
HOVERGENiHBG050763.
InParanoidiQ9JIS7.

Family and domain databases

Gene3Di1.20.120.350. 5 hits.
InterProiIPR031688. CAC1F_C.
IPR027359. Channel_four-helix_dom.
IPR031649. GPHH_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR030157. VDCC_L_a1F.
IPR005446. VDCC_L_a1su.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF184. PTHR10037:SF184. 1 hit.
PfamiPF08763. Ca_chan_IQ. 1 hit.
PF16885. CAC1F_C. 1 hit.
PF16905. GPHH. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01630. LVDCCALPHA1.
SMARTiSM01062. Ca_chan_IQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESEVGKDT TPEPSPANGT GPGPEWGLCP GPPTVGTDTS GASGLGTPRR
60 70 80 90 100
RTQHNKHKTV AVASAQRSPR ALFCLTLTNP IRRSCISIVE WKPFDILILL
110 120 130 140 150
TIFANCVALG VYIPFPEDDS NTANHNLEQV EYVFLVIFTV ETVLKIVAYG
160 170 180 190 200
LVLHPSAYIR NGWNLLDFII VVVGLFSVLL EQGPGRPGDA PHTGGKPGGF
210 220 230 240 250
DVKALRAFRV LRPLRLVSGV PSLHIVVNSI MKALVPLLHI ALLVLFVIII
260 270 280 290 300
YAIIGLELFL GRMHKTCYFL GSDMEAEEDP SPCASSGSGR SCTLNHTECR
310 320 330 340 350
GRWPGPNGGI TNFDNFFFAM LTVFQCITME GWTDVLYWMQ DAMGYELPWV
360 370 380 390 400
YFVSLVIFGS FFVLNLVLGV LSGEFSKERE KAKARGDFQK LREKQQMEED
410 420 430 440 450
LRGYLDWITQ AEELDLHDPS VDGNLASLAE EGRAGHRPQL SELTNRRRGR
460 470 480 490 500
LRWFSHSTRS THSTSSHASL PASDTGSMTD TPGDEDEEEG TMASCTRCLN
510 520 530 540 550
KIMKTRICRH FRRANRGLRA RCRRAVKSNA CYWAVLLLVF LNTLTIASEH
560 570 580 590 600
HGQPLWLTQT QEYANKVLLC LFTVEMLLKL YGLGPSVYVA SFFNRFDCFV
610 620 630 640 650
VCGGILETTL VEVGAMQPLG ISVLRCVRLL RIFKVTRHWA SLSNLVASLL
660 670 680 690 700
NSMKSIASLL LLLFLFIIIF SLLGMQLFGG KFNFDQTHTK RSTFDTFPQA
710 720 730 740 750
LLTVFQILTG EDWNVVMYDG IMAYGGPFFP GMLVCVYFII LFICGNYILL
760 770 780 790 800
NVFLAIAVDN LASGDAGTAK DKGREKSSEG NPPKENKVLV PGGENEDAKG
810 820 830 840 850
ARSEGAAPGM EEEEEEEEEE EEEEEEEEEN GAGHVELLQE VVPKEKVVPI
860 870 880 890 900
PEGSAFFCLS QTNPLRKACH TLIHHHIFTS LILVFIILSS VSLAAEDPIR
910 920 930 940 950
AHSFRNHILG YFDYAFTSIF TVEILLKMTV FGAFLHRGSF CRSWFNLLDL
960 970 980 990 1000
LVVSVSLISF GIHSSAISVV KILRVLRVLR PLRAINRAKG LKHVVQCVFV
1010 1020 1030 1040 1050
AIRTIGNIMI VTTLLQFMFA CIGVQLFKGK FYSCTDEAKH TLKESKGSFL
1060 1070 1080 1090 1100
IYPDGDVSRP LVRERLWVNS DFNFDNVLSA MMALFTVSTF EGWPALLYKA
1110 1120 1130 1140 1150
IDANAEDEGP IYNYHVEISV FFIVYIIIIA FFMMNIFVGF VIITFRAQGE
1160 1170 1180 1190 1200
QEYQNCELDK NQRQCVEYAL KAQPLRRYIP KNPHQYRVWA TVNSRAFEYL
1210 1220 1230 1240 1250
MFLLILLNTV ALAMQHYEQT APFNYAMDIL NMVFTGLFTI EMVLKIIAFK
1260 1270 1280 1290 1300
PKHYFADAWN TFDALIVVGS VVDIAVTEVN NGGHLGESSE DTSRISITFF
1310 1320 1330 1340 1350
RLFRVMRLVK LLSKGEGIRT LLWTFIKSFQ ALPYVALLIA MIFFIYAVIG
1360 1370 1380 1390 1400
MQMFGLVALQ DGTQINRNNN FQTFPQAVLL LFRCATGEAW QEIMLASLPG
1410 1420 1430 1440 1450
NRCDPESDFG PGEEFTCGSS FAIVYFISFF MLCAFLIINL FVAVIMDNFD
1460 1470 1480 1490 1500
YLTRDWSILG PHHLDEFKRI WSEYDPGAKG RIKHLDVVAL LRRIQPPLGF
1510 1520 1530 1540 1550
GKLCPHRVAC KRLVAMNVPL NSDGTVTFNA TLFALVRTSL KIKTEGNLDQ
1560 1570 1580 1590 1600
ANQELRMVIK KIWKRIKQKL LDEVIPPPDE EEVTVGKFYA TFLIQDYFRK
1610 1620 1630 1640 1650
FRRRKEKGLL GREAPTSTSS ALQAGLRSLQ DLGPEIRQAL TYVTEEEEEE
1660 1670 1680 1690 1700
EEAVGQEAEE EEAENNPEPY KDSIDSQPQS RWNSRISVSL PVKEKLPDSL
1710 1720 1730 1740 1750
STGPSDDDGL APNSRQPSVI QAGSQPHRRS SGVFMFTIPE EGSIQLKGTQ
1760 1770 1780 1790 1800
GQDNQNEEQE LPDWTPDLDR AGRDSFEPSP FTTSLVQQHV NGHMSTPTFA
1810 1820 1830 1840 1850
APHACRSEPS FTIQCLQRLG SCEDLPIPGT YHRGRTSGPS RAQGSWAAPP
1860 1870 1880 1890 1900
QKGRLLYAPL LLVEESTVGE GYLGKLGGPL RTFTCLQVPG AHPNPSHRKR
1910 1920 1930 1940 1950
GSADSLVEAV LISEGLGLFA QDPRFVALAK QEIADACHLT LDEMDSAASD
1960 1970 1980
LLAQRTISLY SDEESILSRF DEEDLGDEMA CVHAL
Length:1,985
Mass (Da):221,926
Last modified:October 1, 2000 - v1
Checksum:iC365A105614ACAD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF192497 mRNA. Translation: AAF86764.1.
UniGeneiMm.289647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF192497 mRNA. Translation: AAF86764.1.
UniGeneiMm.289647.

3D structure databases

ProteinModelPortaliQ9JIS7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61285N.
MINTiMINT-4998297.
STRINGi10090.ENSMUSP00000111391.

Chemistry databases

ChEMBLiCHEMBL2176791.
GuidetoPHARMACOLOGYi531.

PTM databases

iPTMnetiQ9JIS7.
PhosphoSitePlusiQ9JIS7.

Proteomic databases

MaxQBiQ9JIS7.
PaxDbiQ9JIS7.
PRIDEiQ9JIS7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1859639. Cacna1f.

Phylogenomic databases

eggNOGiKOG2301. Eukaryota.
ENOG410XNP6. LUCA.
HOGENOMiHOG000231529.
HOVERGENiHBG050763.
InParanoidiQ9JIS7.

Enzyme and pathway databases

ReactomeiR-MMU-419037. NCAM1 interactions.

Miscellaneous databases

PROiQ9JIS7.
SOURCEiSearch...

Family and domain databases

Gene3Di1.20.120.350. 5 hits.
InterProiIPR031688. CAC1F_C.
IPR027359. Channel_four-helix_dom.
IPR031649. GPHH_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR030157. VDCC_L_a1F.
IPR005446. VDCC_L_a1su.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF184. PTHR10037:SF184. 1 hit.
PfamiPF08763. Ca_chan_IQ. 1 hit.
PF16885. CAC1F_C. 1 hit.
PF16905. GPHH. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01630. LVDCCALPHA1.
SMARTiSM01062. Ca_chan_IQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAC1F_MOUSE
AccessioniPrimary (citable) accession number: Q9JIS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.