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Protein

Synaptic vesicle glycoprotein 2A

Gene

Sv2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.
Receptor for the botulinium neurotoxin type A/BOTA.

GO - Molecular functioni

  • protein kinase binding Source: ParkinsonsUK-UCL
  • receptor activity Source: MGI
  • transmembrane transporter activity Source: InterPro

GO - Biological processi

  • cellular calcium ion homeostasis Source: MGI
  • chemical synaptic transmission Source: MGI
  • neurotransmitter transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Neurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicle glycoprotein 2A
Short name:
Synaptic vesicle protein 2
Short name:
Synaptic vesicle protein 2A
Alternative name(s):
Calcium regulator SV2A
Gene namesi
Name:Sv2a
Synonyms:Kiaa0736, Sv2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1927139. Sv2a.

Subcellular locationi

  • Cell junctionsynapse 1 Publication
  • Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane By similarity; Multi-pass membrane protein By similarity

  • Note: Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas (By similarity). Colocalizes with ATP2B1 at photoreceptor synaptic terminals.By similarity1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 169169CytoplasmicSequence analysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence analysisAdd
BLAST
Topological domaini191 – 20515ExtracellularSequence analysisAdd
BLAST
Transmembranei206 – 22621HelicalSequence analysisAdd
BLAST
Topological domaini227 – 2337CytoplasmicSequence analysis
Transmembranei234 – 25421HelicalSequence analysisAdd
BLAST
Topological domaini255 – 2628ExtracellularSequence analysis
Transmembranei263 – 28321HelicalSequence analysisAdd
BLAST
Topological domaini284 – 29411CytoplasmicSequence analysisAdd
BLAST
Transmembranei295 – 31521HelicalSequence analysisAdd
BLAST
Topological domaini316 – 33419ExtracellularSequence analysisAdd
BLAST
Transmembranei335 – 35521HelicalSequence analysisAdd
BLAST
Topological domaini356 – 44792CytoplasmicSequence analysisAdd
BLAST
Transmembranei448 – 46821HelicalSequence analysisAdd
BLAST
Topological domaini469 – 598130ExtracellularSequence analysisAdd
BLAST
Transmembranei599 – 61921HelicalSequence analysisAdd
BLAST
Topological domaini620 – 6267CytoplasmicSequence analysis
Transmembranei627 – 64721HelicalSequence analysisAdd
BLAST
Topological domaini648 – 6514ExtracellularSequence analysis
Transmembranei652 – 67221HelicalSequence analysisAdd
BLAST
Topological domaini673 – 68513CytoplasmicSequence analysisAdd
BLAST
Transmembranei686 – 70823HelicalSequence analysisAdd
BLAST
Topological domaini709 – 7124ExtracellularSequence analysis
Transmembranei713 – 73119HelicalSequence analysisAdd
BLAST
Topological domaini732 – 74211CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell-cell junction Source: MGI
  • cytoplasm Source: MGI
  • endoplasmic reticulum Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • neuromuscular junction Source: MGI
  • neuron projection Source: BHF-UCL
  • presynaptic active zone Source: BHF-UCL
  • synapse Source: MGI
  • synaptic vesicle Source: MGI
  • synaptic vesicle membrane Source: UniProtKB-SubCell
  • terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice fail to grow, experience severe epileptic seizures and die immediately or shortly after birth probably due to multiple neural and endocrine deficits. Mice lacking both Sv2a and Sv2b display a similar phenotype.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 742742Synaptic vesicle glycoprotein 2APRO_0000239766Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei80 – 801PhosphoserineCombined sources
Modified residuei81 – 811PhosphoserineCombined sources
Modified residuei84 – 841PhosphothreonineCombined sources
Modified residuei127 – 1271PhosphoserineCombined sources
Modified residuei393 – 3931PhosphoserineBy similarity
Modified residuei480 – 4801PhosphotyrosineCombined sources
Glycosylationi498 – 4981N-linked (GlcNAc...)Sequence analysis
Glycosylationi548 – 5481N-linked (GlcNAc...)Sequence analysis
Glycosylationi573 – 5731N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.By similarity
N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9JIS5.
PaxDbiQ9JIS5.
PeptideAtlasiQ9JIS5.
PRIDEiQ9JIS5.

PTM databases

iPTMnetiQ9JIS5.
PhosphoSiteiQ9JIS5.
SwissPalmiQ9JIS5.

Expressioni

Tissue specificityi

Expressed in conventional synapses and cone ribbon synapses in the retina (at protein level).1 Publication

Developmental stagei

Expressed during synaptogenesis in the retina (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000038486.
CleanExiMM_SV2A.
GenevisibleiQ9JIS5. MM.

Interactioni

Subunit structurei

Interacts with SYT1/synaptotagmin-1 in a calcium-dependent manner. Binds the adapter protein complex AP-2 (By similarity).By similarity

GO - Molecular functioni

  • protein kinase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi211019. 1 interaction.
IntActiQ9JIS5. 5 interactions.
MINTiMINT-4129805.
STRINGi10090.ENSMUSP00000037576.

Structurei

3D structure databases

ProteinModelPortaliQ9JIS5.
SMRiQ9JIS5. Positions 168-396, 497-577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 5757Interaction with SYT1By similarityAdd
BLAST
Regioni543 – 58038BOTA-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IRID. Eukaryota.
ENOG410YQME. LUCA.
GeneTreeiENSGT00550000074384.
HOGENOMiHOG000065727.
HOVERGENiHBG053967.
InParanoidiQ9JIS5.
KOiK06258.
OMAiMSCISCF.
OrthoDBiEOG091G02NC.
PhylomeDBiQ9JIS5.
TreeFamiTF324824.

Family and domain databases

CDDicd06174. MFS. 2 hits.
InterProiIPR001646. 5peptide_repeat.
IPR011701. MFS.
IPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR005829. Sugar_transporter_CS.
IPR022308. SV2.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
PF13599. Pentapeptide_4. 1 hit.
PF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR01299. synapt_SV2. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEGFRDRAA FIRGAKDIAK EVKKHAAKKV VKGLDRVQDE YSRRSYSRFE
60 70 80 90 100
EEDDDDDFPA PADGYYRGEG AQDEEEGGAS SDATEGHDED DEIYEGEYQG
110 120 130 140 150
IPRAESGGKG ERMADGAPLA GVRGGLSDGE GPPGGRGEAQ RRKDREELAQ
160 170 180 190 200
QYETILRECG HGRFQWTLYF VLGLALMADG VEVFVVGFVL PSAEKDMCLS
210 220 230 240 250
DSNKGMLGLI VYLGMMVGAF LWGGLADRLG RRQCLLISLS VNSVFAFFSS
260 270 280 290 300
FVQGYGTFLF CRLLSGVGIG GSIPIVFSYF SEFLAQEKRG EHLSWLCMFW
310 320 330 340 350
MIGGVYAAAM AWAIIPHYGW SFQMGSAYQF HSWRVFVLVC AFPSVFAIGA
360 370 380 390 400
LTTQPESPRF FLENGKHDEA WMVLKQVHDT NMRAKGHPER VFSVTHIKTI
410 420 430 440 450
HQEDELIEIQ SDTGTWYQRW GVRALSLGGQ VWGNFLSCFS PEYRRITLMM
460 470 480 490 500
MGVWFTMSFS YYGLTVWFPD MIRHLQAVDY AARTKVFPGE RVEHVTFNFT
510 520 530 540 550
LENQIHRGGQ YFNDKFIGLR LKSVSFEDSL FEECYFEDVT SSNTFFRNCT
560 570 580 590 600
FINTVFYNTD LFEYKFVNSR LVNSTFLHNK EGCPLDVTGT GEGAYMVYFV
610 620 630 640 650
SFLGTLAVLP GNIVSALLMD KIGRLRMLAG SSVLSCVSCF FLSFGNSESA
660 670 680 690 700
MIALLCLFGG VSIASWNALD VLTVELYPSD KRTTAFGFLN ALCKLAAVLG
710 720 730 740
ISIFTSFVGI TKAAPILFAS AALALGSSLA LKLPETRGQV LQ
Length:742
Mass (Da):82,647
Last modified:October 1, 2000 - v1
Checksum:i1074857FD13ED894
GO

Sequence cautioni

The sequence BAC65642 differs from that shown. Reason: Frameshift at position 72. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF196781, AF196780 Genomic DNA. Translation: AAF87321.1.
AK122360 Transcribed RNA. Translation: BAC65642.3. Sequence problems.
AK028318 mRNA. Translation: BAC25876.1.
BC026494 mRNA. Translation: AAH26494.1.
BC046587 mRNA. Translation: AAH46587.1.
CCDSiCCDS17631.1.
RefSeqiNP_071313.1. NM_022030.3.
UniGeneiMm.491135.

Genome annotation databases

EnsembliENSMUST00000035371; ENSMUSP00000037576; ENSMUSG00000038486.
GeneIDi64051.
KEGGimmu:64051.
UCSCiuc008qmf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF196781, AF196780 Genomic DNA. Translation: AAF87321.1.
AK122360 Transcribed RNA. Translation: BAC65642.3. Sequence problems.
AK028318 mRNA. Translation: BAC25876.1.
BC026494 mRNA. Translation: AAH26494.1.
BC046587 mRNA. Translation: AAH46587.1.
CCDSiCCDS17631.1.
RefSeqiNP_071313.1. NM_022030.3.
UniGeneiMm.491135.

3D structure databases

ProteinModelPortaliQ9JIS5.
SMRiQ9JIS5. Positions 168-396, 497-577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211019. 1 interaction.
IntActiQ9JIS5. 5 interactions.
MINTiMINT-4129805.
STRINGi10090.ENSMUSP00000037576.

PTM databases

iPTMnetiQ9JIS5.
PhosphoSiteiQ9JIS5.
SwissPalmiQ9JIS5.

Proteomic databases

MaxQBiQ9JIS5.
PaxDbiQ9JIS5.
PeptideAtlasiQ9JIS5.
PRIDEiQ9JIS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035371; ENSMUSP00000037576; ENSMUSG00000038486.
GeneIDi64051.
KEGGimmu:64051.
UCSCiuc008qmf.1. mouse.

Organism-specific databases

CTDi9900.
MGIiMGI:1927139. Sv2a.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IRID. Eukaryota.
ENOG410YQME. LUCA.
GeneTreeiENSGT00550000074384.
HOGENOMiHOG000065727.
HOVERGENiHBG053967.
InParanoidiQ9JIS5.
KOiK06258.
OMAiMSCISCF.
OrthoDBiEOG091G02NC.
PhylomeDBiQ9JIS5.
TreeFamiTF324824.

Miscellaneous databases

PROiQ9JIS5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038486.
CleanExiMM_SV2A.
GenevisibleiQ9JIS5. MM.

Family and domain databases

CDDicd06174. MFS. 2 hits.
InterProiIPR001646. 5peptide_repeat.
IPR011701. MFS.
IPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR005829. Sugar_transporter_CS.
IPR022308. SV2.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
PF13599. Pentapeptide_4. 1 hit.
PF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR01299. synapt_SV2. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSV2A_MOUSE
AccessioniPrimary (citable) accession number: Q9JIS5
Secondary accession number(s): Q80TT0, Q8R0R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.