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Q9JIQ3

- DBLOH_MOUSE

UniProt

Q9JIQ3 - DBLOH_MOUSE

Protein

Diablo homolog, mitochondrial

Gene

Diablo

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases By similarity.By similarity

    GO - Biological processi

    1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
    2. activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: MGI
    3. intrinsic apoptotic signaling pathway Source: UniProtKB
    4. intrinsic apoptotic signaling pathway in response to oxidative stress Source: MGI
    5. neuron apoptotic process Source: MGI
    6. positive regulation of apoptotic process Source: UniProtKB

    Keywords - Biological processi

    Apoptosis

    Enzyme and pathway databases

    ReactomeiREACT_211550. Release of apoptotic factors from the mitochondria.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diablo homolog, mitochondrial
    Alternative name(s):
    Direct IAP-binding protein with low pI
    Second mitochondria-derived activator of caspase
    Short name:
    Smac
    Gene namesi
    Name:Diablo
    Synonyms:Smac
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1913843. Diablo.

    Subcellular locationi

    Mitochondrion 1 Publication
    Note: Released into the cytosol when cells undergo apoptosis.

    GO - Cellular componenti

    1. CD40 receptor complex Source: BHF-UCL
    2. cytoplasm Source: MGI
    3. cytoplasmic side of plasma membrane Source: BHF-UCL
    4. mitochondrial intermembrane space Source: UniProtKB
    5. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5353MitochondrionBy similarityAdd
    BLAST
    Chaini54 – 237184Diablo homolog, mitochondrialPRO_0000021073Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by BIRC7/livin.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    MaxQBiQ9JIQ3.
    PaxDbiQ9JIQ3.
    PRIDEiQ9JIQ3.

    PTM databases

    PhosphoSiteiQ9JIQ3.

    Expressioni

    Tissue specificityi

    Highest expression found in heart, liver, kidney and testis.1 Publication

    Gene expression databases

    ArrayExpressiQ9JIQ3.
    CleanExiMM_DIABLO.
    GenevestigatoriQ9JIQ3.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin By similarity. Interacts with NGFRAP1/BEX3.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi211579. 2 interactions.
    IntActiQ9JIQ3. 2 interactions.
    MINTiMINT-1341305.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JIQ3.
    SMRiQ9JIQ3. Positions 54-237.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi54 – 585IAP-bindingSequence Analysis

    Domaini

    The mature N-terminus mediates interaction with XIAP/BIRC4.By similarity

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiNOG42122.
    GeneTreeiENSGT00390000007237.
    HOGENOMiHOG000217916.
    HOVERGENiHBG051315.
    InParanoidiQ9JIQ3.
    KOiK10522.
    OMAiQKLEPHS.
    OrthoDBiEOG7SJD66.
    PhylomeDBiQ9JIQ3.
    TreeFamiTF102048.

    Family and domain databases

    InterProiIPR015142. Smac_DIABLO.
    IPR009062. Smac_DIABLO-like.
    [Graphical view]
    PfamiPF09057. Smac_DIABLO. 1 hit.
    [Graphical view]
    SUPFAMiSSF46984. SSF46984. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9JIQ3-1 [UniParc]FASTAAdd to Basket

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    MAALRSWVTR SVCSLFRYRQ RFPVLANSKK RCFSELIKPW HKTVLTGFGM    50
    TLCAVPIAQK SEPQSLSNEA LMRRAVSLVT DSTSTFLSQT TYALIEAITE 100
    YTKAVYTLVS LYRQYTSLLG KMNSQEEDEV WQVIIGARVE MTSKQQEYLK 150
    LETTWMTAVG LSEMAAEAAY QTGADQASIT ARNHIQLVKS QVQEVRQLSQ 200
    KAETKLAEAQ TKELHQKAQE VSDEGADQEE EAYLRED 237
    Length:237
    Mass (Da):26,820
    Last modified:October 3, 2012 - v2
    Checksum:i6961A7E76C780AC5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641Q → H in AAF82190. (PubMed:10929712)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF203914 mRNA. Translation: AAF82190.1.
    AK012760 mRNA. Translation: BAB28450.1.
    AK002887 mRNA. No translation available.
    AK077198 mRNA. Translation: BAC36676.1.
    AK133194 mRNA. Translation: BAE21552.1.
    AC157931 Genomic DNA. No translation available.
    CCDSiCCDS39270.1.
    RefSeqiNP_075721.3. NM_023232.3.
    UniGeneiMm.46716.

    Genome annotation databases

    EnsembliENSMUST00000111587; ENSMUSP00000107214; ENSMUSG00000029433.
    ENSMUST00000125652; ENSMUSP00000115045; ENSMUSG00000029433.
    GeneIDi66593.
    KEGGimmu:66593.
    UCSCiuc008znx.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF203914 mRNA. Translation: AAF82190.1 .
    AK012760 mRNA. Translation: BAB28450.1 .
    AK002887 mRNA. No translation available.
    AK077198 mRNA. Translation: BAC36676.1 .
    AK133194 mRNA. Translation: BAE21552.1 .
    AC157931 Genomic DNA. No translation available.
    CCDSi CCDS39270.1.
    RefSeqi NP_075721.3. NM_023232.3.
    UniGenei Mm.46716.

    3D structure databases

    ProteinModelPortali Q9JIQ3.
    SMRi Q9JIQ3. Positions 54-237.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211579. 2 interactions.
    IntActi Q9JIQ3. 2 interactions.
    MINTi MINT-1341305.

    PTM databases

    PhosphoSitei Q9JIQ3.

    Proteomic databases

    MaxQBi Q9JIQ3.
    PaxDbi Q9JIQ3.
    PRIDEi Q9JIQ3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000111587 ; ENSMUSP00000107214 ; ENSMUSG00000029433 .
    ENSMUST00000125652 ; ENSMUSP00000115045 ; ENSMUSG00000029433 .
    GeneIDi 66593.
    KEGGi mmu:66593.
    UCSCi uc008znx.1. mouse.

    Organism-specific databases

    CTDi 56616.
    MGIi MGI:1913843. Diablo.

    Phylogenomic databases

    eggNOGi NOG42122.
    GeneTreei ENSGT00390000007237.
    HOGENOMi HOG000217916.
    HOVERGENi HBG051315.
    InParanoidi Q9JIQ3.
    KOi K10522.
    OMAi QKLEPHS.
    OrthoDBi EOG7SJD66.
    PhylomeDBi Q9JIQ3.
    TreeFami TF102048.

    Enzyme and pathway databases

    Reactomei REACT_211550. Release of apoptotic factors from the mitochondria.

    Miscellaneous databases

    ChiTaRSi DIABLO. mouse.
    NextBioi 322104.
    PROi Q9JIQ3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JIQ3.
    CleanExi MM_DIABLO.
    Genevestigatori Q9JIQ3.

    Family and domain databases

    InterProi IPR015142. Smac_DIABLO.
    IPR009062. Smac_DIABLO-like.
    [Graphical view ]
    Pfami PF09057. Smac_DIABLO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46984. SSF46984. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins."
      Verhagen A.M., Ekert P.G., Pakusch M., Silke J., Connolly L.M., Reid G.E., Moritz R.L., Simpson R.J., Vaux D.L.
      Cell 102:43-53(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Kidney.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis."
      Yoon K., Jang H.D., Lee S.Y.
      Biochem. Biophys. Res. Commun. 319:649-654(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NGFRAP1.

    Entry informationi

    Entry nameiDBLOH_MOUSE
    AccessioniPrimary (citable) accession number: Q9JIQ3
    Secondary accession number(s): Q542V8, Q9CZD1, Q9DCD3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 18, 2001
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3