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Q9JIQ3 (DBLOH_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diablo homolog, mitochondrial
Alternative name(s):
Direct IAP-binding protein with low pI
Second mitochondria-derived activator of caspase
Short name=Smac
Gene names
Name:Diablo
Synonyms:Smac
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases By similarity. Ref.1

Subunit structure

Homodimer. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin By similarity. Interacts with NGFRAP1/BEX3. Ref.4

Subcellular location

Mitochondrion. Note: Released into the cytosol when cells undergo apoptosis. Ref.1

Tissue specificity

Highest expression found in heart, liver, kidney and testis. Ref.1

Domain

The mature N-terminus mediates interaction with XIAP/BIRC4 By similarity.

Post-translational modification

Ubiquitinated by BIRC7/livin By similarity.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5353Mitochondrion By similarity
Chain54 – 237184Diablo homolog, mitochondrial
PRO_0000021073

Regions

Motif54 – 585IAP-binding Potential

Experimental info

Sequence conflict641Q → H in AAF82190. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9JIQ3 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: 6961A7E76C780AC5

FASTA23726,820
        10         20         30         40         50         60 
MAALRSWVTR SVCSLFRYRQ RFPVLANSKK RCFSELIKPW HKTVLTGFGM TLCAVPIAQK 

        70         80         90        100        110        120 
SEPQSLSNEA LMRRAVSLVT DSTSTFLSQT TYALIEAITE YTKAVYTLVS LYRQYTSLLG 

       130        140        150        160        170        180 
KMNSQEEDEV WQVIIGARVE MTSKQQEYLK LETTWMTAVG LSEMAAEAAY QTGADQASIT 

       190        200        210        220        230 
ARNHIQLVKS QVQEVRQLSQ KAETKLAEAQ TKELHQKAQE VSDEGADQEE EAYLRED 

« Hide

References

« Hide 'large scale' references
[1]"Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins."
Verhagen A.M., Ekert P.G., Pakusch M., Silke J., Connolly L.M., Reid G.E., Moritz R.L., Simpson R.J., Vaux D.L.
Cell 102:43-53(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Kidney.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Testis.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis."
Yoon K., Jang H.D., Lee S.Y.
Biochem. Biophys. Res. Commun. 319:649-654(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NGFRAP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF203914 mRNA. Translation: AAF82190.1.
AK012760 mRNA. Translation: BAB28450.1.
AK002887 mRNA. No translation available.
AK077198 mRNA. Translation: BAC36676.1.
AK133194 mRNA. Translation: BAE21552.1.
AC157931 Genomic DNA. No translation available.
CCDSCCDS39270.1.
RefSeqNP_075721.3. NM_023232.3.
UniGeneMm.46716.

3D structure databases

ProteinModelPortalQ9JIQ3.
SMRQ9JIQ3. Positions 54-237.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid211579. 2 interactions.
IntActQ9JIQ3. 2 interactions.
MINTMINT-1341305.

PTM databases

PhosphoSiteQ9JIQ3.

Proteomic databases

MaxQBQ9JIQ3.
PaxDbQ9JIQ3.
PRIDEQ9JIQ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000111587; ENSMUSP00000107214; ENSMUSG00000029433.
ENSMUST00000125652; ENSMUSP00000115045; ENSMUSG00000029433.
GeneID66593.
KEGGmmu:66593.
UCSCuc008znx.1. mouse.

Organism-specific databases

CTD56616.
MGIMGI:1913843. Diablo.

Phylogenomic databases

eggNOGNOG42122.
GeneTreeENSGT00390000007237.
HOGENOMHOG000217916.
HOVERGENHBG051315.
InParanoidQ9JIQ3.
KOK10522.
OMAQKLEPHS.
OrthoDBEOG7SJD66.
PhylomeDBQ9JIQ3.
TreeFamTF102048.

Gene expression databases

ArrayExpressQ9JIQ3.
CleanExMM_DIABLO.
GenevestigatorQ9JIQ3.

Family and domain databases

InterProIPR015142. Smac_DIABLO.
IPR009062. Smac_DIABLO-like.
[Graphical view]
PfamPF09057. Smac_DIABLO. 1 hit.
[Graphical view]
SUPFAMSSF46984. SSF46984. 1 hit.
ProtoNetSearch...

Other

ChiTaRSDIABLO. mouse.
NextBio322104.
PROQ9JIQ3.
SOURCESearch...

Entry information

Entry nameDBLOH_MOUSE
AccessionPrimary (citable) accession number: Q9JIQ3
Secondary accession number(s): Q542V8, Q9CZD1, Q9DCD3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot