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Protein

Transient receptor potential cation channel subfamily V member 5

Gene

Trpv5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constitutively active calcium selective cation channel thought to be involved in Ca2+ reabsorption in kidney and intestine (PubMed:10875938). Required for normal Ca2+ reabsorption in the kidney distal convoluted tubules (By similarity). The channel is activated by low internal calcium level and the current exhibits an inward rectification (By similarity). A Ca2+-dependent feedback regulation includes fast channel inactivation and slow current decay (By similarity). Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).By similarity1 Publication

Enzyme regulationi

Activated by WNK3.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi535Calcium; shared with neighboring subunitsBy similarity1

GO - Molecular functioni

  • calcium channel activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • calcium ion import into cell Source: UniProtKB
  • calcium ion transmembrane transport Source: UniProtKB
  • calcium ion transport Source: UniProtKB
  • calcium ion transport into cytosol Source: RGD
  • protein tetramerization Source: UniProtKB
  • regulation of urine volume Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Calmodulin-binding, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-3295583. TRP channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily V member 5
Short name:
TrpV5
Alternative name(s):
Calcium transporter 21 Publication
Short name:
CaT21 Publication
Epithelial calcium channel 1
Short name:
ECaC1
Osm-9-like TRP channel 3
Short name:
OTRPC3
Gene namesi
Name:Trpv5
Synonyms:Cat2, Ecac, Ecac1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi620636. Trpv5.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein Curated
  • Apical cell membrane By similarity; Multi-pass membrane protein By similarity

  • Note: Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells (By similarity). The expression of the glycosylated form in the cell membrane is increased in the presence of WNK3 (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 320CytoplasmicSequence analysisAdd BLAST320
Transmembranei321 – 341HelicalSequence analysisAdd BLAST21
Topological domaini342 – 380ExtracellularSequence analysisAdd BLAST39
Transmembranei381 – 401HelicalSequence analysisAdd BLAST21
Topological domaini402 – 412CytoplasmicSequence analysisAdd BLAST11
Transmembranei413 – 433HelicalSequence analysisAdd BLAST21
Topological domaini434 – 441ExtracellularSequence analysis8
Transmembranei442 – 462HelicalSequence analysisAdd BLAST21
Topological domaini463 – 485CytoplasmicSequence analysisAdd BLAST23
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21
Intramembranei517 – 537Pore-formingCuratedAdd BLAST21
Transmembranei550 – 570HelicalSequence analysisAdd BLAST21
Topological domaini571 – 723CytoplasmicSequence analysisAdd BLAST153

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • intrinsic component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153531 – 723Transient receptor potential cation channel subfamily V member 5Add BLAST723

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi351N-linked (GlcNAc...)Sequence analysis1
Modified residuei678PhosphothreonineBy similarity1
Modified residuei682PhosphoserineBy similarity1

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9JIP0.
PRIDEiQ9JIP0.

Expressioni

Tissue specificityi

Detected in kidney (at protein level). Detected in kidney.1 Publication

Gene expression databases

BgeeiENSRNOG00000015394.
GenevisibleiQ9JIP0. RN.

Interactioni

Subunit structurei

Homotetramer and probably heterotetramer with TRPV6. Interacts with TRPV6 (By similarity). Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with calmodulin. Interacts with BSPRY, which results in its inactivation (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020975.

Structurei

3D structure databases

ProteinModelPortaliQ9JIP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati72 – 101ANK 1Sequence analysisAdd BLAST30
Repeati110 – 139ANK 2Sequence analysisAdd BLAST30
Repeati156 – 185ANK 3Sequence analysisAdd BLAST30
Repeati189 – 222ANK 4Sequence analysisAdd BLAST34
Repeati232 – 261ANK 5Sequence analysisAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni591 – 595Interaction with S100A10By similarity5
Regioni643 – 646Involved in Ca(2+)-dependent inactivationBy similarity4
Regioni693 – 723Involved in Ca(2+)-dependent inactivationBy similarityAdd BLAST31

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
GeneTreeiENSGT00550000074425.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiQ9JIP0.
KOiK04974.
OMAiVNLPFMF.
OrthoDBiEOG091G0314.
PhylomeDBiQ9JIP0.
TreeFamiTF314711.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008346. TRPV5.
IPR008344. TRPV5/TRPV6.
[Graphical view]
PANTHERiPTHR10582:SF11. PTHR10582:SF11. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01767. ECACCHANNEL2.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JIP0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVKKPWIQL QKRLNWWVRE QDWNQHVDQL HMLQQKSIWE SPLLRAAKEN
60 70 80 90 100
DMCTLKRLQH DQNCDFRQRG ALGETALHVA ALYDNLDAAI MLMETAPYLV
110 120 130 140 150
TESTLCEPFV GQTALHIAIM NQNVNLVRAL LARGASASAR ATGSAFHRSS
160 170 180 190 200
HNLIYYGEHP LSFAACVGSE EIVRLLIEHG ADIRAQDSLG NTVLHILVLQ
210 220 230 240 250
PNKTFACQMY NLLLSHDGGD HLKSLELVPN NQGLTPFKLA GVEGNTVMFQ
260 270 280 290 300
HLMQKRKHIQ WSLGPLTSSI YDLTEIDSWG EDLSFLELVV SSKKKEARQI
310 320 330 340 350
LEQTPVKELV SLKWKKYGQP YFCLLGMLYI FYMICFTTCC VYRPLKFRDA
360 370 380 390 400
NRTHVRDNTV LEQKPLQEAY VTYQDKVRLV GELVTVIGAV VILLIEIPDI
410 420 430 440 450
FRVGASRYFG HTVLGGPFHV IIITYASLVL LIMVMRLTSM NGEVVPISMA
460 470 480 490 500
LVLGWCSVMY FSRGFQMLGP FTIMIQKMIF GDLLRFCWLM AMVILGFASA
510 520 530 540 550
FYIIFQTEDP ESLGEFSDYP TAMFSTFELF LTIIDGPANY SVDLPFMYHL
560 570 580 590 600
TYFAFAIIAT LLMLNLFIAM MGDTHWRVAQ ERDELWRAQV VATTVMLERK
610 620 630 640 650
MPRFLWPRSG ICGCEYGLGD RWFLRVEHHQ EQNPYRVLRY VEAFKSSDKE
660 670 680 690 700
EVQEQLSEKQ PSGTETGTLA RGSVVLQTPP LSRTTSLSSN SHRGWEILRR
710 720
NTLGHLNLGQ DLGEGDGEEI YHF
Length:723
Mass (Da):82,454
Last modified:October 1, 2000 - v1
Checksum:iF41FCF2F2B431A25
GO
Isoform 2 (identifier: Q9JIP0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-296: Missing.

Show »
Length:674
Mass (Da):76,691
Checksum:i692B148D08FE9BA3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti290V → L in BAA99541 (Ref. 2) Curated1
Sequence conflicti295K → I in BAA99541 (Ref. 2) Curated1
Sequence conflicti331F → S in BAA99541 (Ref. 2) Curated1
Sequence conflicti488W → R in BAA99541 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013438248 – 296Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF209196 mRNA. Translation: AAF86309.1.
AB032019 mRNA. Translation: BAA99541.1.
AY762624 mRNA. Translation: AAV31121.1.
RefSeqiNP_446239.2. NM_053787.2.
UniGeneiRn.137513.

Genome annotation databases

EnsembliENSRNOT00000020975; ENSRNOP00000020975; ENSRNOG00000015394. [Q9JIP0-1]
ENSRNOT00000051687; ENSRNOP00000046276; ENSRNOG00000015394. [Q9JIP0-2]
GeneIDi116469.
KEGGirno:116469.
UCSCiRGD:620636. rat. [Q9JIP0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF209196 mRNA. Translation: AAF86309.1.
AB032019 mRNA. Translation: BAA99541.1.
AY762624 mRNA. Translation: AAV31121.1.
RefSeqiNP_446239.2. NM_053787.2.
UniGeneiRn.137513.

3D structure databases

ProteinModelPortaliQ9JIP0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020975.

Proteomic databases

PaxDbiQ9JIP0.
PRIDEiQ9JIP0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020975; ENSRNOP00000020975; ENSRNOG00000015394. [Q9JIP0-1]
ENSRNOT00000051687; ENSRNOP00000046276; ENSRNOG00000015394. [Q9JIP0-2]
GeneIDi116469.
KEGGirno:116469.
UCSCiRGD:620636. rat. [Q9JIP0-1]

Organism-specific databases

CTDi56302.
RGDi620636. Trpv5.

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
GeneTreeiENSGT00550000074425.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiQ9JIP0.
KOiK04974.
OMAiVNLPFMF.
OrthoDBiEOG091G0314.
PhylomeDBiQ9JIP0.
TreeFamiTF314711.

Enzyme and pathway databases

ReactomeiR-RNO-3295583. TRP channels.

Miscellaneous databases

PROiQ9JIP0.

Gene expression databases

BgeeiENSRNOG00000015394.
GenevisibleiQ9JIP0. RN.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008346. TRPV5.
IPR008344. TRPV5/TRPV6.
[Graphical view]
PANTHERiPTHR10582:SF11. PTHR10582:SF11. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01767. ECACCHANNEL2.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPV5_RAT
AccessioniPrimary (citable) accession number: Q9JIP0
Secondary accession number(s): Q5UC98, Q9JJL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.