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Q9JIP0 (TRPV5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Transient receptor potential cation channel subfamily V member 5
Short name=TrpV5
Alternative name(s):
Calcium transporter 2
Short name=CaT2
Epithelial calcium channel 1
Short name=ECaC1
Osm-9-like TRP channel 3
Short name=OTRPC3
Gene names
Name:Trpv5
Synonyms:Cat2, Ecac, Ecac1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length723 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Constitutively active calcium selective cation channel thought to be involved in Ca2+ reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca2+-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating By similarity. Ref.1

Enzyme regulation

Activated by WNK3 By similarity.

Subunit structure

Homotetramer and probably heterotetramer with TRPV6. Interacts with TRPV6. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with calmodulin. Interacts with BSPRY, which results in its inactivation By similarity.

Subcellular location

Apical cell membrane; Multi-pass membrane protein By similarity. Note: Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells. The expression of the glycosylated form in the cell membrane is increased in the presence of WNK3 By similarity.

Tissue specificity

Exclusively expressed in kidney. Ref.1

Post-translational modification

Glycosylated By similarity.

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV5 sub-subfamily. [View classification]

Contains 4 ANK repeats.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JIP0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JIP0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     248-296: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 723723Transient receptor potential cation channel subfamily V member 5
PRO_0000215353

Regions

Topological domain1 – 320320Cytoplasmic Potential
Transmembrane321 – 34121Helical; Potential
Topological domain342 – 38039Extracellular Potential
Transmembrane381 – 40121Helical; Potential
Topological domain402 – 41312Cytoplasmic Potential
Transmembrane414 – 43421Helical; Potential
Topological domain435 – 4417Extracellular Potential
Transmembrane442 – 46221Helical; Potential
Topological domain463 – 48523Cytoplasmic Potential
Transmembrane486 – 50621Helical; Potential
Intramembrane517 – 53923Pore-forming; Probable
Transmembrane552 – 57221Helical; Potential
Topological domain573 – 723151Cytoplasmic Potential
Repeat72 – 10130ANK 1
Repeat110 – 13930ANK 2
Repeat156 – 18530ANK 3
Repeat232 – 26130ANK 4
Region591 – 5955Interaction with S100A10 By similarity
Region643 – 6464Involved in Ca(2+)-dependent inactivation By similarity
Region693 – 72331Involved in Ca(2+)-dependent inactivation By similarity

Amino acid modifications

Glycosylation3511N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence248 – 29649Missing in isoform 2.
VSP_013438

Experimental info

Sequence conflict2901V → L in BAA99541. Ref.2
Sequence conflict2951K → I in BAA99541. Ref.2
Sequence conflict3311F → S in BAA99541. Ref.2
Sequence conflict4881W → R in BAA99541. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: F41FCF2F2B431A25

FASTA72382,454
        10         20         30         40         50         60 
MGVKKPWIQL QKRLNWWVRE QDWNQHVDQL HMLQQKSIWE SPLLRAAKEN DMCTLKRLQH 

        70         80         90        100        110        120 
DQNCDFRQRG ALGETALHVA ALYDNLDAAI MLMETAPYLV TESTLCEPFV GQTALHIAIM 

       130        140        150        160        170        180 
NQNVNLVRAL LARGASASAR ATGSAFHRSS HNLIYYGEHP LSFAACVGSE EIVRLLIEHG 

       190        200        210        220        230        240 
ADIRAQDSLG NTVLHILVLQ PNKTFACQMY NLLLSHDGGD HLKSLELVPN NQGLTPFKLA 

       250        260        270        280        290        300 
GVEGNTVMFQ HLMQKRKHIQ WSLGPLTSSI YDLTEIDSWG EDLSFLELVV SSKKKEARQI 

       310        320        330        340        350        360 
LEQTPVKELV SLKWKKYGQP YFCLLGMLYI FYMICFTTCC VYRPLKFRDA NRTHVRDNTV 

       370        380        390        400        410        420 
LEQKPLQEAY VTYQDKVRLV GELVTVIGAV VILLIEIPDI FRVGASRYFG HTVLGGPFHV 

       430        440        450        460        470        480 
IIITYASLVL LIMVMRLTSM NGEVVPISMA LVLGWCSVMY FSRGFQMLGP FTIMIQKMIF 

       490        500        510        520        530        540 
GDLLRFCWLM AMVILGFASA FYIIFQTEDP ESLGEFSDYP TAMFSTFELF LTIIDGPANY 

       550        560        570        580        590        600 
SVDLPFMYHL TYFAFAIIAT LLMLNLFIAM MGDTHWRVAQ ERDELWRAQV VATTVMLERK 

       610        620        630        640        650        660 
MPRFLWPRSG ICGCEYGLGD RWFLRVEHHQ EQNPYRVLRY VEAFKSSDKE EVQEQLSEKQ 

       670        680        690        700        710        720 
PSGTETGTLA RGSVVLQTPP LSRTTSLSSN SHRGWEILRR NTLGHLNLGQ DLGEGDGEEI 


YHF

« Hide

Isoform 2 [UniParc].

Checksum: 692B148D08FE9BA3
Show »

FASTA67476,691

References

[1]"A rat kidney-specific calcium transporter in the distal nephron."
Peng J.-B., Chen X.-Z., Berger U.V., Vassilev P.M., Brown E.M., Hediger M.A.
J. Biol. Chem. 275:28186-28194(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
Strain: CD1 Charles River.
Tissue: Kidney cortex.
[2]Ishibashi K., Suzuki M., Imai M.
Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Kidney.
[3]"A variant of the TrpV5 calcium channel from rat."
Nehrke K., Sherman T., Bushinsky D.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: Sprague-Dawley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF209196 mRNA. Translation: AAF86309.1.
AB032019 mRNA. Translation: BAA99541.1.
AY762624 mRNA. Translation: AAV31121.1.
IPIIPI00200811.
IPI00480697.
RefSeqNP_446239.2. NM_053787.2.
UniGeneRn.137513.

3D structure databases

ProteinModelPortalQ9JIP0.
SMRQ9JIP0. Positions 38-259.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000020975.

Proteomic databases

PRIDEQ9JIP0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000020975; ENSRNOP00000020975; ENSRNOG00000015394.
ENSRNOT00000051687; ENSRNOP00000046276; ENSRNOG00000015394.
GeneID116469.
KEGGrno:116469.
UCSCRGD:620636. rat.

Organism-specific databases

CTD56302.
RGD620636. Trpv5.

Phylogenomic databases

eggNOGNOG278734.
GeneTreeENSGT00550000074425.
HOGENOMHOG000234397.
HOVERGENHBG061442.
InParanoidQ9JIP0.
KOK04974.
OMAENHHDQN.
OrthoDBEOG4SBDXG.

Gene expression databases

GenevestigatorQ9JIP0.
GermOnlineENSRNOG00000015394. Rattus norvegicus.

Family and domain databases

Gene3D1.25.40.20. 2 hits.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008344. TRPV5/TRPV6_channel.
IPR008346. TRPV5_channel.
IPR024862. TRPV_channel.
[Graphical view]
PANTHERPTHR10582. PTHR10582. 1 hit.
PTHR10582:SF11. PTHR10582:SF11. 1 hit.
PfamPF12796. Ank_2. 2 hits.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01767. ECACCHANNEL2.
SMARTSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMSSF48403. ANK. 1 hit.
TIGRFAMsTIGR00870. trp. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio619014.

Entry information

Entry nameTRPV5_RAT
AccessionPrimary (citable) accession number: Q9JIP0
Secondary accession number(s): Q5UC98, Q9JJL2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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