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Q9JIN6 (KCMB4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-activated potassium channel subunit beta-4
Alternative name(s):
BK channel subunit beta-4
Short name=BKbeta4
Calcium-activated potassium channel, subfamily M subunit beta-4
Charybdotoxin receptor subunit beta-4
K(VCA)beta-4
Maxi K channel subunit beta-4
Slo-beta-4
Gene names
Name:Kcnmb4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Decreases the gating kinetics and calcium sensitivity of the KCNMA1 channel, but with fast deactivation kinetics. May decrease KCNMA1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. Makes KCNMA1 channel resistant to 100 nM charybdotoxin (CTX) toxin concentrations By similarity.

Subunit structure

Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB4 per KCNMA1 tetramer. Ref.1

Subcellular location

Membrane; Multi-pass membrane protein By similarity.

Domain

Resistance to charybdotoxin (CTX) toxin is mediated by the extracellular domain By similarity.

Post-translational modification

Phosphorylated. Phosphorylation modulates its effect on KCNMA1 activation kinetics By similarity.

N-glycosylated. A highly glycosylated form is promoted by KCNMA1. Glycosylation, which is not required for the interaction with KCNMA1 and subcellular location, increases protection against charybdotoxin By similarity.

Sequence similarities

Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB4 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210Calcium-activated potassium channel subunit beta-4
PRO_0000187056

Regions

Topological domain1 – 1919Cytoplasmic Potential
Transmembrane20 – 4021Helical; Name=1; Potential
Topological domain41 – 167127Extracellular Potential
Transmembrane168 – 18821Helical; Name=2; Potential
Topological domain189 – 21022Cytoplasmic Potential

Amino acid modifications

Glycosylation531N-linked (GlcNAc...) By similarity
Glycosylation901N-linked (GlcNAc...) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9JIN6 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 11C73DD2C2C6F1D4

FASTA21023,857
        10         20         30         40         50         60 
MAKLRVSYEY TEAEDKSIRL GLFLIVSGIL SLFIFGFCWL SPALQDLQAT AANCTVLSVQ 

        70         80         90        100        110        120 
QIGEVFECTF TCGTDCRGTS QYPCVQVYVN NSESNSRALL HSDQHQLLTN PKCSYIPPCK 

       130        140        150        160        170        180 
RENQKNSESV MNWQQYWKDE IGSQPFTCYF NQHQRPEDVL LQRTHDEIAL LHCFLWPVVA 

       190        200        210 
FVVGVLIVVL TICAKSLAVK AEAMKKRKFS 

« Hide

References

« Hide 'large scale' references
[1]"A novel nervous system beta subunit that downregulates human large conductance calcium-dependent potassium channels."
Weiger T.M., Holmqvist M.H., Levitan I.B., Clark F.T., Sprague S., Huang W.-J., Ge P., Wang C., Lawson D., Jurman M.E., Glucksmann M.A., Silos-Santiago I., DiStefano P.S., Curtis R.
J. Neurosci. 20:3563-3570(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH KCNMA1.
Strain: BALB/c.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF215892 mRNA. Translation: AAF75597.1.
BC117779 mRNA. Translation: AAI17780.1.
CCDSCCDS24184.1.
RefSeqNP_067427.1. NM_021452.1.
UniGeneMm.440652.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB8.A.14.1.1. the ca(2+)-activated k(+) channel auxiliary subunit slowpoke- (slo) family.

PTM databases

PhosphoSiteQ9JIN6.

Proteomic databases

PRIDEQ9JIN6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000068233; ENSMUSP00000065384; ENSMUSG00000054934.
GeneID58802.
KEGGmmu:58802.
UCSCuc007hbw.1. mouse.

Organism-specific databases

CTD27345.
MGIMGI:1913272. Kcnmb4.

Phylogenomic databases

eggNOGNOG83271.
GeneTreeENSGT00390000015997.
HOGENOMHOG000113205.
HOVERGENHBG052224.
InParanoidQ149I1.
KOK04941.
OMAYVPPCER.
OrthoDBEOG77WWDC.
PhylomeDBQ9JIN6.
TreeFamTF328589.

Gene expression databases

ArrayExpressQ9JIN6.
BgeeQ9JIN6.
GenevestigatorQ9JIN6.

Family and domain databases

InterProIPR003930. K_chnl_Ca-activ_BK_bsu.
[Graphical view]
PANTHERPTHR10258. PTHR10258. 1 hit.
PfamPF03185. CaKB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSKCNMB4. mouse.
NextBio314378.
PROQ9JIN6.
SOURCESearch...

Entry information

Entry nameKCMB4_MOUSE
AccessionPrimary (citable) accession number: Q9JIN6
Secondary accession number(s): Q149I1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot