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Q9JIM4 (DUS12_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity protein phosphatase 12

EC=3.1.3.16
EC=3.1.3.48
Alternative name(s):
Glucokinase-associated dual specificity phosphatase
Short name=GKAP
Gene names
Name:Dusp12
Synonyms:Gkap
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Seems to dephosphorylate glucokinase. Ref.1

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subcellular location

Cytoplasm Ref.1.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Contains 1 tyrosine-protein phosphatase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 339339Dual specificity protein phosphatase 12
PRO_0000260313

Regions

Domain87 – 16175Tyrosine-protein phosphatase

Sites

Active site1141Phosphocysteine intermediate By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9JIM4 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: F2E41DE35F0E2C00

FASTA33937,196
        10         20         30         40         50         60 
MLEVQSSNHG CERQAPTTSP ASSAGHAVEV RPGLYLGGAA AVAGPDYLRE AGITAVLTVD 

        70         80         90        100        110        120 
SEPAFPAGAG FEGLQSLFVP ALDKPETDLL SHLDRCVAFI GQARSEGRAV LVHCHAGVSR 

       130        140        150        160        170        180 
SVAVVTAFIM KTEQLTFEKA YENLQTIKPE AKMNEGFEWQ LKLYEAMGHE VHTSSAVYKQ 

       190        200        210        220        230        240 
YRLQKVTEKY PELRNLPREL FAVDPTTVSQ GLKDDILYKC RKCRRSLFRR SSILDHSEGS 

       250        260        270        280        290        300 
GPVAFAHKRT GLSSVLTTGN QAQCTSYFIE PVQWMESALL GVMDGQLLCP KCSAKLGSFN 

       310        320        330 
WYGEQCSCGR WITPAFQIHK NRVDEVKTLP ALGSQTKKP 

« Hide

References

[1]"A novel cytosolic dual specificity phosphatase, interacting with glucokinase, increases glucose phosphorylation rate."
Munoz-Alonso M.J., Guillemain G., Kassis N., Girard J., Burnol A.-F., Leturque A.
J. Biol. Chem. 275:32406-32412(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF217233 mRNA. Translation: AAF87971.1.
RefSeqNP_071584.1. NM_022248.1.
UniGeneRn.52231.

3D structure databases

ProteinModelPortalQ9JIM4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000004179.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000004179; ENSRNOP00000004179; ENSRNOG00000003100.
GeneID64014.
KEGGrno:64014.
UCSCRGD:68375. rat.

Organism-specific databases

CTD11266.
RGD68375. Dusp12.

Phylogenomic databases

eggNOGCOG2453.
GeneTreeENSGT00740000115610.
HOGENOMHOG000243638.
HOVERGENHBG051421.
InParanoidQ9JIM4.
KOK14819.
OMAKITHILT.
OrthoDBEOG7Z95MG.
PhylomeDBQ9JIM4.
TreeFamTF105123.

Gene expression databases

GenevestigatorQ9JIM4.

Family and domain databases

Gene3D3.90.190.10. 1 hit.
InterProIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR016278. DUSP12.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
[Graphical view]
PANTHERPTHR10159. PTHR10159. 1 hit.
PfamPF00782. DSPc. 1 hit.
[Graphical view]
PIRSFPIRSF000941. DUSP12. 1 hit.
SMARTSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
PROSITEPS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio612590.
PROQ9JIM4.

Entry information

Entry nameDUS12_RAT
AccessionPrimary (citable) accession number: Q9JIM4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families