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Protein

Dual specificity protein phosphatase 12

Gene

Dusp12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Seems to dephosphorylate glucokinase.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141Phosphocysteine intermediateBy similarity

GO - Molecular functioni

  1. kinase binding Source: RGD
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoprotein phosphatase activity Source: RGD
  4. protein tyrosine/serine/threonine phosphatase activity Source: GO_Central
  5. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. positive regulation of glucokinase activity Source: RGD
  2. protein dephosphorylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 12 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Glucokinase-associated dual specificity phosphatase
Short name:
GKAP
Gene namesi
Name:Dusp12
Synonyms:Gkap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi68375. Dusp12.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Dual specificity protein phosphatase 12PRO_0000260313Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Expressioni

Gene expression databases

GenevestigatoriQ9JIM4.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004179.

Structurei

3D structure databases

ProteinModelPortaliQ9JIM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 16175Tyrosine-protein phosphataseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00740000115610.
HOGENOMiHOG000243638.
HOVERGENiHBG051421.
InParanoidiQ9JIM4.
KOiK14819.
OMAiFAWQGMQ.
OrthoDBiEOG7Z95MG.
PhylomeDBiQ9JIM4.
TreeFamiTF105123.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR016278. DUSP12.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PIRSFiPIRSF000941. DUSP12. 1 hit.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEVQSSNHG CERQAPTTSP ASSAGHAVEV RPGLYLGGAA AVAGPDYLRE
60 70 80 90 100
AGITAVLTVD SEPAFPAGAG FEGLQSLFVP ALDKPETDLL SHLDRCVAFI
110 120 130 140 150
GQARSEGRAV LVHCHAGVSR SVAVVTAFIM KTEQLTFEKA YENLQTIKPE
160 170 180 190 200
AKMNEGFEWQ LKLYEAMGHE VHTSSAVYKQ YRLQKVTEKY PELRNLPREL
210 220 230 240 250
FAVDPTTVSQ GLKDDILYKC RKCRRSLFRR SSILDHSEGS GPVAFAHKRT
260 270 280 290 300
GLSSVLTTGN QAQCTSYFIE PVQWMESALL GVMDGQLLCP KCSAKLGSFN
310 320 330
WYGEQCSCGR WITPAFQIHK NRVDEVKTLP ALGSQTKKP
Length:339
Mass (Da):37,196
Last modified:September 30, 2000 - v1
Checksum:iF2E41DE35F0E2C00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217233 mRNA. Translation: AAF87971.1.
RefSeqiNP_071584.1. NM_022248.1.
UniGeneiRn.52231.

Genome annotation databases

EnsembliENSRNOT00000004179; ENSRNOP00000004179; ENSRNOG00000003100.
GeneIDi64014.
KEGGirno:64014.
UCSCiRGD:68375. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217233 mRNA. Translation: AAF87971.1.
RefSeqiNP_071584.1. NM_022248.1.
UniGeneiRn.52231.

3D structure databases

ProteinModelPortaliQ9JIM4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004179; ENSRNOP00000004179; ENSRNOG00000003100.
GeneIDi64014.
KEGGirno:64014.
UCSCiRGD:68375. rat.

Organism-specific databases

CTDi11266.
RGDi68375. Dusp12.

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00740000115610.
HOGENOMiHOG000243638.
HOVERGENiHBG051421.
InParanoidiQ9JIM4.
KOiK14819.
OMAiFAWQGMQ.
OrthoDBiEOG7Z95MG.
PhylomeDBiQ9JIM4.
TreeFamiTF105123.

Miscellaneous databases

NextBioi612590.
PROiQ9JIM4.

Gene expression databases

GenevestigatoriQ9JIM4.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR016278. DUSP12.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PIRSFiPIRSF000941. DUSP12. 1 hit.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel cytosolic dual specificity phosphatase, interacting with glucokinase, increases glucose phosphorylation rate."
    Munoz-Alonso M.J., Guillemain G., Kassis N., Girard J., Burnol A.-F., Leturque A.
    J. Biol. Chem. 275:32406-32412(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDUS12_RAT
AccessioniPrimary (citable) accession number: Q9JIM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 27, 2006
Last sequence update: September 30, 2000
Last modified: March 3, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.