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Q9JIM1

- S29A1_MOUSE

UniProt

Q9JIM1 - S29A1_MOUSE

Protein

Equilibrative nucleoside transporter 1

Gene

Slc29a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).

    GO - Molecular functioni

    1. nucleoside transmembrane transporter activity Source: MGI

    GO - Biological processi

    1. cellular response to glucose stimulus Source: Ensembl
    2. cellular response to hypoxia Source: Ensembl
    3. lactation Source: Ensembl
    4. nucleoside transmembrane transport Source: GOC
    5. nucleoside transport Source: MGI
    6. regulation of excitatory postsynaptic membrane potential Source: Ensembl
    7. sleep Source: Ensembl
    8. uridine transport Source: Ensembl

    Keywords - Biological processi

    Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Equilibrative nucleoside transporter 1
    Alternative name(s):
    Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
    Short name:
    Equilibrative NBMPR-sensitive nucleoside transporter
    Nucleoside transporter, es-type
    Solute carrier family 29 member 1
    Gene namesi
    Name:Slc29a1
    Synonyms:Ent1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1927073. Slc29a1.

    Subcellular locationi

    Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity. Apical cell membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: Predominantly localized in the basolateral membrane in polarized MDCK cells.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. basolateral plasma membrane Source: UniProtKB
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 460459Equilibrative nucleoside transporter 1PRO_0000209338Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi48 – 481N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9JIM1.
    PRIDEiQ9JIM1.

    PTM databases

    PhosphoSiteiQ9JIM1.

    Expressioni

    Tissue specificityi

    Highly expressed in heart, spleen, lung, liver and testis. Lower level of expression in brain and kidney.1 Publication

    Gene expression databases

    ArrayExpressiQ9JIM1.
    BgeeiQ9JIM1.
    GenevestigatoriQ9JIM1.

    Interactioni

    Subunit structurei

    Identified in a complex with STOM.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000063096.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JIM1.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1211CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini30 – 8253ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini108 – 1114CytoplasmicSequence Analysis
    Topological domaini131 – 1388ExtracellularSequence Analysis
    Topological domaini158 – 17417CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini200 – 2067ExtracellularSequence Analysis
    Topological domaini228 – 29164CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini312 – 32312ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini343 – 35917CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini379 – 39719ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini418 – 43518CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini457 – 4604ExtracellularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 2917HelicalSequence AnalysisAdd
    BLAST
    Transmembranei83 – 10725HelicalSequence AnalysisAdd
    BLAST
    Transmembranei112 – 13019HelicalSequence AnalysisAdd
    BLAST
    Transmembranei139 – 15719HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19925HelicalSequence AnalysisAdd
    BLAST
    Transmembranei207 – 22721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei292 – 31120HelicalSequence AnalysisAdd
    BLAST
    Transmembranei324 – 34219HelicalSequence AnalysisAdd
    BLAST
    Transmembranei360 – 37819HelicalSequence AnalysisAdd
    BLAST
    Transmembranei398 – 41720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei436 – 45621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249415.
    GeneTreeiENSGT00390000002232.
    HOGENOMiHOG000007684.
    HOVERGENiHBG074626.
    InParanoidiQ9JIM1.
    KOiK15014.
    OMAiRIANWNI.
    OrthoDBiEOG7PP56W.
    PhylomeDBiQ9JIM1.
    TreeFamiTF313950.

    Family and domain databases

    InterProiIPR002259. Eqnu_transpt.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PANTHERiPTHR10332. PTHR10332. 1 hit.
    PfamiPF01733. Nucleoside_tran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016379. ENT. 1 hit.
    PRINTSiPR01130. DERENTRNSPRT.
    SUPFAMiSSF103473. SSF103473. 3 hits.
    TIGRFAMsiTIGR00939. 2a57. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JIM1-1) [UniParc]FASTAAdd to Basket

    Also known as: Ent1b

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFMTATKYFT NRLDVSQNVS    50
    SDTDQSCEST KALADPTVAL PARSSLSAIF NNVMTLCAML PLLVFTCLNS 100
    FLHQRISQSV RILGSLLAIL LVFLVTAALV KVEMDALIFF VITMIKIVLI 150
    NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ GLAGFFTSVA MICAIASGSE 200
    LSESAFGYFI TACAVVILAI LCYLALPRTE FYRHYLQLNL AGPAEQETKL 250
    DLISKGEEPK GRREESGVPG PNSPPTNRNQ SIKAILKSIC VPALSVCFIF 300
    TVTIGLFPAV TAEVESSIAG TSPWKSYFIP VACFLNFNVF DWLGRSLTAV 350
    CMWPGQDSRW LPVLVASRIV FIPLLMLCNV KARHCGAQRH HFVFKHDAWF 400
    IAFMAAFAFS NGYLASLCMC FGPKKVKPAE AETAGNIMSF FLCLGLALGA 450
    VLSFLLRALV 460
    Length:460
    Mass (Da):50,192
    Last modified:January 23, 2007 - v3
    Checksum:iFE37EEA155F9AB2F
    GO
    Isoform 2 (identifier: Q9JIM1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         254-256: SKG → R

    Show »
    Length:458
    Mass (Da):50,076
    Checksum:iEDE0FDEF5BF3E274
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti54 – 541D → G in BAB23537. (PubMed:16141072)Curated
    Sequence conflicti138 – 1381I → T in AAH04828. (PubMed:15489334)Curated
    Sequence conflicti372 – 3721I → V in AAH04828. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei254 – 2563SKG → R in isoform 2. 3 PublicationsVSP_010471

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF218255 Genomic DNA. Translation: AAF64035.2.
    AF218255 Genomic DNA. Translation: AAF64036.2.
    AF305501 mRNA. Translation: AAG22828.1.
    AF131212 mRNA. Translation: AAF78452.1.
    AK004756 mRNA. Translation: BAB23537.1.
    AK050089 mRNA. Translation: BAC34063.1.
    BC004828 mRNA. Translation: AAH04828.1.
    BC006812 mRNA. Translation: AAH06812.1.
    CCDSiCCDS28813.1. [Q9JIM1-2]
    CCDS57096.1. [Q9JIM1-1]
    RefSeqiNP_001186042.1. NM_001199113.1. [Q9JIM1-1]
    NP_001186043.1. NM_001199114.1. [Q9JIM1-1]
    NP_001186044.1. NM_001199115.1. [Q9JIM1-2]
    NP_001186045.1. NM_001199116.1. [Q9JIM1-2]
    NP_075018.1. NM_022880.3. [Q9JIM1-2]
    XP_006524831.1. XM_006524768.1. [Q9JIM1-1]
    XP_006524832.1. XM_006524769.1. [Q9JIM1-1]
    XP_006524833.1. XM_006524770.1. [Q9JIM1-1]
    XP_006524834.1. XM_006524771.1. [Q9JIM1-2]
    UniGeneiMm.29744.
    Mm.486335.

    Genome annotation databases

    EnsembliENSMUST00000051574; ENSMUSP00000063096; ENSMUSG00000023942. [Q9JIM1-1]
    ENSMUST00000064889; ENSMUSP00000063757; ENSMUSG00000023942. [Q9JIM1-2]
    ENSMUST00000097317; ENSMUSP00000094923; ENSMUSG00000023942. [Q9JIM1-1]
    ENSMUST00000163492; ENSMUSP00000129242; ENSMUSG00000023942. [Q9JIM1-2]
    ENSMUST00000166119; ENSMUSP00000128763; ENSMUSG00000023942. [Q9JIM1-1]
    ENSMUST00000167692; ENSMUSP00000131976; ENSMUSG00000023942. [Q9JIM1-1]
    ENSMUST00000171847; ENSMUSP00000126703; ENSMUSG00000023942. [Q9JIM1-2]
    GeneIDi63959.
    KEGGimmu:63959.
    UCSCiuc008crb.2. mouse. [Q9JIM1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF218255 Genomic DNA. Translation: AAF64035.2 .
    AF218255 Genomic DNA. Translation: AAF64036.2 .
    AF305501 mRNA. Translation: AAG22828.1 .
    AF131212 mRNA. Translation: AAF78452.1 .
    AK004756 mRNA. Translation: BAB23537.1 .
    AK050089 mRNA. Translation: BAC34063.1 .
    BC004828 mRNA. Translation: AAH04828.1 .
    BC006812 mRNA. Translation: AAH06812.1 .
    CCDSi CCDS28813.1. [Q9JIM1-2 ]
    CCDS57096.1. [Q9JIM1-1 ]
    RefSeqi NP_001186042.1. NM_001199113.1. [Q9JIM1-1 ]
    NP_001186043.1. NM_001199114.1. [Q9JIM1-1 ]
    NP_001186044.1. NM_001199115.1. [Q9JIM1-2 ]
    NP_001186045.1. NM_001199116.1. [Q9JIM1-2 ]
    NP_075018.1. NM_022880.3. [Q9JIM1-2 ]
    XP_006524831.1. XM_006524768.1. [Q9JIM1-1 ]
    XP_006524832.1. XM_006524769.1. [Q9JIM1-1 ]
    XP_006524833.1. XM_006524770.1. [Q9JIM1-1 ]
    XP_006524834.1. XM_006524771.1. [Q9JIM1-2 ]
    UniGenei Mm.29744.
    Mm.486335.

    3D structure databases

    ProteinModelPortali Q9JIM1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000063096.

    Chemistry

    ChEMBLi CHEMBL1287611.

    PTM databases

    PhosphoSitei Q9JIM1.

    Proteomic databases

    PaxDbi Q9JIM1.
    PRIDEi Q9JIM1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000051574 ; ENSMUSP00000063096 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
    ENSMUST00000064889 ; ENSMUSP00000063757 ; ENSMUSG00000023942 . [Q9JIM1-2 ]
    ENSMUST00000097317 ; ENSMUSP00000094923 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
    ENSMUST00000163492 ; ENSMUSP00000129242 ; ENSMUSG00000023942 . [Q9JIM1-2 ]
    ENSMUST00000166119 ; ENSMUSP00000128763 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
    ENSMUST00000167692 ; ENSMUSP00000131976 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
    ENSMUST00000171847 ; ENSMUSP00000126703 ; ENSMUSG00000023942 . [Q9JIM1-2 ]
    GeneIDi 63959.
    KEGGi mmu:63959.
    UCSCi uc008crb.2. mouse. [Q9JIM1-1 ]

    Organism-specific databases

    CTDi 2030.
    MGIi MGI:1927073. Slc29a1.

    Phylogenomic databases

    eggNOGi NOG249415.
    GeneTreei ENSGT00390000002232.
    HOGENOMi HOG000007684.
    HOVERGENi HBG074626.
    InParanoidi Q9JIM1.
    KOi K15014.
    OMAi RIANWNI.
    OrthoDBi EOG7PP56W.
    PhylomeDBi Q9JIM1.
    TreeFami TF313950.

    Miscellaneous databases

    NextBioi 319829.
    PROi Q9JIM1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JIM1.
    Bgeei Q9JIM1.
    Genevestigatori Q9JIM1.

    Family and domain databases

    InterProi IPR002259. Eqnu_transpt.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    PANTHERi PTHR10332. PTHR10332. 1 hit.
    Pfami PF01733. Nucleoside_tran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016379. ENT. 1 hit.
    PRINTSi PR01130. DERENTRNSPRT.
    SUPFAMi SSF103473. SSF103473. 3 hits.
    TIGRFAMsi TIGR00939. 2a57. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genomic organization and expression of the mouse equilibrative, nitrobenzylthioinosine-sensitive nucleoside transporter 1 (ENT1) gene."
      Choi D.-S., Handa M., Young H., Gordon A.S., Diamond I., Messing R.O.
      Biochem. Biophys. Res. Commun. 277:200-208(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
      Strain: 129/SvJ.
    2. "Molecular cloning and functional characterization of inhibitor-sensitive (mENT1) and inhibitor-resistant (mENT2) equilibrative nucleoside transporters from mouse brain."
      Kiss A., Farah K., Kim J., Garriock R.J., Drysdale T.A., Hammond J.R.
      Biochem. J. 352:363-372(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
      Strain: CD-1.
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Liver and Lung.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Mammary tumor.
    5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-48.

    Entry informationi

    Entry nameiS29A1_MOUSE
    AccessioniPrimary (citable) accession number: Q9JIM1
    Secondary accession number(s): Q99K84, Q9DBT8, Q9JHF0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 99 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3