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Protein

Equilibrative nucleoside transporter 1

Gene

Slc29a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).

GO - Molecular functioni

  • nucleoside transmembrane transporter activity Source: MGI

GO - Biological processi

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-MMU-83936. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 1
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
Short name:
Equilibrative NBMPR-sensitive nucleoside transporter
Nucleoside transporter, es-type
Solute carrier family 29 member 1
Gene namesi
Name:Slc29a1
Synonyms:Ent1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1927073. Slc29a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 29HelicalSequence analysisAdd BLAST17
Topological domaini30 – 82ExtracellularSequence analysisAdd BLAST53
Transmembranei83 – 107HelicalSequence analysisAdd BLAST25
Topological domaini108 – 111CytoplasmicSequence analysis4
Transmembranei112 – 130HelicalSequence analysisAdd BLAST19
Topological domaini131 – 138ExtracellularSequence analysis8
Transmembranei139 – 157HelicalSequence analysisAdd BLAST19
Topological domaini158 – 174CytoplasmicSequence analysisAdd BLAST17
Transmembranei175 – 199HelicalSequence analysisAdd BLAST25
Topological domaini200 – 206ExtracellularSequence analysis7
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 291CytoplasmicSequence analysisAdd BLAST64
Transmembranei292 – 311HelicalSequence analysisAdd BLAST20
Topological domaini312 – 323ExtracellularSequence analysisAdd BLAST12
Transmembranei324 – 342HelicalSequence analysisAdd BLAST19
Topological domaini343 – 359CytoplasmicSequence analysisAdd BLAST17
Transmembranei360 – 378HelicalSequence analysisAdd BLAST19
Topological domaini379 – 397ExtracellularSequence analysisAdd BLAST19
Transmembranei398 – 417HelicalSequence analysisAdd BLAST20
Topological domaini418 – 435CytoplasmicSequence analysisAdd BLAST18
Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
Topological domaini457 – 460ExtracellularSequence analysis4

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1287611.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002093381 – 460Equilibrative nucleoside transporter 1Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi48N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei254PhosphoserineBy similarity1
Modified residuei273PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9JIM1.
PaxDbiQ9JIM1.
PRIDEiQ9JIM1.

PTM databases

iPTMnetiQ9JIM1.
PhosphoSitePlusiQ9JIM1.

Expressioni

Tissue specificityi

Highly expressed in heart, spleen, lung, liver and testis. Lower level of expression in brain and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000023942.
ExpressionAtlasiQ9JIM1. baseline and differential.
GenevisibleiQ9JIM1. MM.

Interactioni

Subunit structurei

Identified in a complex with STOM.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063096.

Structurei

3D structure databases

ProteinModelPortaliQ9JIM1.
SMRiQ9JIM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1479. Eukaryota.
ENOG410Y3MT. LUCA.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiQ9JIM1.
KOiK15014.
OMAiFNIFDWL.
OrthoDBiEOG091G09WB.
PhylomeDBiQ9JIM1.
TreeFamiTF313950.

Family and domain databases

InterProiView protein in InterPro
IPR030195. ENT1.
IPR034764. ENT1/ENT2.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF51. PTHR10332:SF51. 1 hit.
PfamiView protein in Pfam
PF01733. Nucleoside_tran. 1 hit.
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JIM1-1) [UniParc]FASTAAdd to basket
Also known as: Ent1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFMTATKYFT NRLDVSQNVS
60 70 80 90 100
SDTDQSCEST KALADPTVAL PARSSLSAIF NNVMTLCAML PLLVFTCLNS
110 120 130 140 150
FLHQRISQSV RILGSLLAIL LVFLVTAALV KVEMDALIFF VITMIKIVLI
160 170 180 190 200
NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ GLAGFFTSVA MICAIASGSE
210 220 230 240 250
LSESAFGYFI TACAVVILAI LCYLALPRTE FYRHYLQLNL AGPAEQETKL
260 270 280 290 300
DLISKGEEPK GRREESGVPG PNSPPTNRNQ SIKAILKSIC VPALSVCFIF
310 320 330 340 350
TVTIGLFPAV TAEVESSIAG TSPWKSYFIP VACFLNFNVF DWLGRSLTAV
360 370 380 390 400
CMWPGQDSRW LPVLVASRIV FIPLLMLCNV KARHCGAQRH HFVFKHDAWF
410 420 430 440 450
IAFMAAFAFS NGYLASLCMC FGPKKVKPAE AETAGNIMSF FLCLGLALGA
460
VLSFLLRALV
Length:460
Mass (Da):50,192
Last modified:January 23, 2007 - v3
Checksum:iFE37EEA155F9AB2F
GO
Isoform 2 (identifier: Q9JIM1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-256: SKG → R

Show »
Length:458
Mass (Da):50,076
Checksum:iEDE0FDEF5BF3E274
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54D → G in BAB23537 (PubMed:16141072).Curated1
Sequence conflicti138I → T in AAH04828 (PubMed:15489334).Curated1
Sequence conflicti372I → V in AAH04828 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010471254 – 256SKG → R in isoform 2. 3 Publications3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218255 Genomic DNA. Translation: AAF64035.2.
AF218255 Genomic DNA. Translation: AAF64036.2.
AF305501 mRNA. Translation: AAG22828.1.
AF131212 mRNA. Translation: AAF78452.1.
AK004756 mRNA. Translation: BAB23537.1.
AK050089 mRNA. Translation: BAC34063.1.
BC004828 mRNA. Translation: AAH04828.1.
BC006812 mRNA. Translation: AAH06812.1.
CCDSiCCDS28813.1. [Q9JIM1-2]
CCDS57096.1. [Q9JIM1-1]
RefSeqiNP_001186042.1. NM_001199113.1. [Q9JIM1-1]
NP_001186043.1. NM_001199114.1. [Q9JIM1-1]
NP_001186044.1. NM_001199115.1. [Q9JIM1-2]
NP_001186045.1. NM_001199116.1. [Q9JIM1-2]
NP_075018.1. NM_022880.3. [Q9JIM1-2]
XP_006524831.1. XM_006524768.1. [Q9JIM1-1]
XP_006524832.1. XM_006524769.2. [Q9JIM1-1]
XP_006524833.1. XM_006524770.3. [Q9JIM1-1]
XP_006524834.1. XM_006524771.1. [Q9JIM1-2]
UniGeneiMm.29744.
Mm.486335.

Genome annotation databases

EnsembliENSMUST00000051574; ENSMUSP00000063096; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000064889; ENSMUSP00000063757; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000097317; ENSMUSP00000094923; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000163492; ENSMUSP00000129242; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000166119; ENSMUSP00000128763; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000167692; ENSMUSP00000131976; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000171847; ENSMUSP00000126703; ENSMUSG00000023942. [Q9JIM1-2]
GeneIDi63959.
KEGGimmu:63959.
UCSCiuc008crb.2. mouse. [Q9JIM1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiS29A1_MOUSE
AccessioniPrimary (citable) accession number: Q9JIM1
Secondary accession number(s): Q99K84, Q9DBT8, Q9JHF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 124 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families