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Q9JIM1 (S29A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Equilibrative nucleoside transporter 1
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
Short name=Equilibrative NBMPR-sensitive nucleoside transporter
Nucleoside transporter, es-type
Solute carrier family 29 member 1
Gene names
Name:Slc29a1
Synonyms:Ent1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).

Subcellular location

Basolateral cell membrane; Multi-pass membrane protein By similarity. Apical cell membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Highly expressed in heart, spleen, lung, liver and testis. Lower level of expression in brain and kidney. Ref.1

Sequence similarities

Belongs to the SLC29A/ENT transporter (TC 2.A.57) family. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JIM1-1)

Also known as: Ent1b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JIM1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     254-256: SKG → R

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 460459Equilibrative nucleoside transporter 1
PRO_0000209338

Regions

Topological domain2 – 1211Cytoplasmic Potential
Transmembrane13 – 2917Helical; Potential
Topological domain30 – 8253Extracellular Potential
Transmembrane83 – 10725Helical; Potential
Topological domain108 – 1114Cytoplasmic Potential
Transmembrane112 – 13019Helical; Potential
Topological domain131 – 1388Extracellular Potential
Transmembrane139 – 15719Helical; Potential
Topological domain158 – 17417Cytoplasmic Potential
Transmembrane175 – 19925Helical; Potential
Topological domain200 – 2067Extracellular Potential
Transmembrane207 – 22721Helical; Potential
Topological domain228 – 29164Cytoplasmic Potential
Transmembrane292 – 31120Helical; Potential
Topological domain312 – 32312Extracellular Potential
Transmembrane324 – 34219Helical; Potential
Topological domain343 – 35917Cytoplasmic Potential
Transmembrane360 – 37819Helical; Potential
Topological domain379 – 39719Extracellular Potential
Transmembrane398 – 41720Helical; Potential
Topological domain418 – 43518Cytoplasmic Potential
Transmembrane436 – 45621Helical; Potential
Topological domain457 – 4604Extracellular Potential

Amino acid modifications

Glycosylation481N-linked (GlcNAc...) Ref.5

Natural variations

Alternative sequence254 – 2563SKG → R in isoform 2.
VSP_010471

Experimental info

Sequence conflict541D → G in BAB23537. Ref.3
Sequence conflict1381I → T in AAH04828. Ref.4
Sequence conflict3721I → V in AAH04828. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Ent1b) [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: FE37EEA155F9AB2F

FASTA46050,192
        10         20         30         40         50         60 
MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFMTATKYFT NRLDVSQNVS SDTDQSCEST 

        70         80         90        100        110        120 
KALADPTVAL PARSSLSAIF NNVMTLCAML PLLVFTCLNS FLHQRISQSV RILGSLLAIL 

       130        140        150        160        170        180 
LVFLVTAALV KVEMDALIFF VITMIKIVLI NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ 

       190        200        210        220        230        240 
GLAGFFTSVA MICAIASGSE LSESAFGYFI TACAVVILAI LCYLALPRTE FYRHYLQLNL 

       250        260        270        280        290        300 
AGPAEQETKL DLISKGEEPK GRREESGVPG PNSPPTNRNQ SIKAILKSIC VPALSVCFIF 

       310        320        330        340        350        360 
TVTIGLFPAV TAEVESSIAG TSPWKSYFIP VACFLNFNVF DWLGRSLTAV CMWPGQDSRW 

       370        380        390        400        410        420 
LPVLVASRIV FIPLLMLCNV KARHCGAQRH HFVFKHDAWF IAFMAAFAFS NGYLASLCMC 

       430        440        450        460 
FGPKKVKPAE AETAGNIMSF FLCLGLALGA VLSFLLRALV 

« Hide

Isoform 2 [UniParc].

Checksum: EDE0FDEF5BF3E274
Show »

FASTA45850,076

References

« Hide 'large scale' references
[1]"Genomic organization and expression of the mouse equilibrative, nitrobenzylthioinosine-sensitive nucleoside transporter 1 (ENT1) gene."
Choi D.-S., Handa M., Young H., Gordon A.S., Diamond I., Messing R.O.
Biochem. Biophys. Res. Commun. 277:200-208(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
Strain: 129/SvJ.
[2]"Molecular cloning and functional characterization of inhibitor-sensitive (mENT1) and inhibitor-resistant (mENT2) equilibrative nucleoside transporters from mouse brain."
Kiss A., Farah K., Kim J., Garriock R.J., Drysdale T.A., Hammond J.R.
Biochem. J. 352:363-372(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
Strain: CD-1.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Liver and Lung.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Mammary tumor.
[5]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-48.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF218255 Genomic DNA. Translation: AAF64035.2.
AF218255 Genomic DNA. Translation: AAF64036.2.
AF305501 mRNA. Translation: AAG22828.1.
AF131212 mRNA. Translation: AAF78452.1.
AK004756 mRNA. Translation: BAB23537.1.
AK050089 mRNA. Translation: BAC34063.1.
BC004828 mRNA. Translation: AAH04828.1.
BC006812 mRNA. Translation: AAH06812.1.
RefSeqNP_001186042.1. NM_001199113.1.
NP_001186043.1. NM_001199114.1.
NP_001186044.1. NM_001199115.1.
NP_001186045.1. NM_001199116.1.
NP_075018.1. NM_022880.3.
XP_006524831.1. XM_006524768.1.
XP_006524832.1. XM_006524769.1.
XP_006524833.1. XM_006524770.1.
XP_006524834.1. XM_006524771.1.
UniGeneMm.29744.
Mm.486335.

3D structure databases

ProteinModelPortalQ9JIM1.
SMRQ9JIM1. Positions 399-449.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000063096.

Chemistry

ChEMBLCHEMBL1287611.

PTM databases

PhosphoSiteQ9JIM1.

Proteomic databases

PaxDbQ9JIM1.
PRIDEQ9JIM1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000051574; ENSMUSP00000063096; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000064889; ENSMUSP00000063757; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000097317; ENSMUSP00000094923; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000163492; ENSMUSP00000129242; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000166119; ENSMUSP00000128763; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000167692; ENSMUSP00000131976; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000171847; ENSMUSP00000126703; ENSMUSG00000023942. [Q9JIM1-2]
GeneID63959.
KEGGmmu:63959.
UCSCuc008crb.2. mouse. [Q9JIM1-1]

Organism-specific databases

CTD2030.
MGIMGI:1927073. Slc29a1.

Phylogenomic databases

eggNOGNOG249415.
GeneTreeENSGT00390000002232.
HOGENOMHOG000007684.
HOVERGENHBG074626.
InParanoidQ9JIM1.
KOK15014.
OMAISQSVRI.
OrthoDBEOG7PP56W.
PhylomeDBQ9JIM1.
TreeFamTF313950.

Gene expression databases

ArrayExpressQ9JIM1.
BgeeQ9JIM1.
GenevestigatorQ9JIM1.

Family and domain databases

InterProIPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PANTHERPTHR10332. PTHR10332. 1 hit.
PfamPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFPIRSF016379. ENT. 1 hit.
PRINTSPR01130. DERENTRNSPRT.
SUPFAMSSF103473. SSF103473. 3 hits.
TIGRFAMsTIGR00939. 2a57. 1 hit.
ProtoNetSearch...

Other

NextBio319829.
PROQ9JIM1.
SOURCESearch...

Entry information

Entry nameS29A1_MOUSE
AccessionPrimary (citable) accession number: Q9JIM1
Secondary accession number(s): Q99K84, Q9DBT8, Q9JHF0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot