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Q9JIM1

- S29A1_MOUSE

UniProt

Q9JIM1 - S29A1_MOUSE

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Protein
Equilibrative nucleoside transporter 1
Gene
Slc29a1, Ent1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).

GO - Molecular functioni

  1. nucleoside transmembrane transporter activity Source: MGI

GO - Biological processi

  1. cellular response to glucose stimulus Source: Ensembl
  2. cellular response to hypoxia Source: Ensembl
  3. lactation Source: Ensembl
  4. nucleoside transmembrane transport Source: GOC
  5. nucleoside transport Source: MGI
  6. regulation of excitatory postsynaptic membrane potential Source: Ensembl
  7. sleep Source: Ensembl
  8. uridine transport Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 1
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
Short name:
Equilibrative NBMPR-sensitive nucleoside transporter
Nucleoside transporter, es-type
Solute carrier family 29 member 1
Gene namesi
Name:Slc29a1
Synonyms:Ent1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1927073. Slc29a1.

Subcellular locationi

Basolateral cell membrane; Multi-pass membrane protein By similarity. Apical cell membrane; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein By similarity
Note: Predominantly localized in the basolateral membrane in polarized MDCK cells By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1211Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei13 – 2917Helical; Reviewed prediction
Add
BLAST
Topological domaini30 – 8253Extracellular Reviewed prediction
Add
BLAST
Transmembranei83 – 10725Helical; Reviewed prediction
Add
BLAST
Topological domaini108 – 1114Cytoplasmic Reviewed prediction
Transmembranei112 – 13019Helical; Reviewed prediction
Add
BLAST
Topological domaini131 – 1388Extracellular Reviewed prediction
Transmembranei139 – 15719Helical; Reviewed prediction
Add
BLAST
Topological domaini158 – 17417Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei175 – 19925Helical; Reviewed prediction
Add
BLAST
Topological domaini200 – 2067Extracellular Reviewed prediction
Transmembranei207 – 22721Helical; Reviewed prediction
Add
BLAST
Topological domaini228 – 29164Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei292 – 31120Helical; Reviewed prediction
Add
BLAST
Topological domaini312 – 32312Extracellular Reviewed prediction
Add
BLAST
Transmembranei324 – 34219Helical; Reviewed prediction
Add
BLAST
Topological domaini343 – 35917Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei360 – 37819Helical; Reviewed prediction
Add
BLAST
Topological domaini379 – 39719Extracellular Reviewed prediction
Add
BLAST
Transmembranei398 – 41720Helical; Reviewed prediction
Add
BLAST
Topological domaini418 – 43518Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei436 – 45621Helical; Reviewed prediction
Add
BLAST
Topological domaini457 – 4604Extracellular Reviewed prediction

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. basolateral plasma membrane Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 460459Equilibrative nucleoside transporter 1
PRO_0000209338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi48 – 481N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9JIM1.
PRIDEiQ9JIM1.

PTM databases

PhosphoSiteiQ9JIM1.

Expressioni

Tissue specificityi

Highly expressed in heart, spleen, lung, liver and testis. Lower level of expression in brain and kidney.1 Publication

Gene expression databases

ArrayExpressiQ9JIM1.
BgeeiQ9JIM1.
GenevestigatoriQ9JIM1.

Interactioni

Subunit structurei

Identified in a complex with STOM By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063096.

Structurei

3D structure databases

ProteinModelPortaliQ9JIM1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiQ9JIM1.
KOiK15014.
OMAiRIANWNI.
OrthoDBiEOG7PP56W.
PhylomeDBiQ9JIM1.
TreeFamiTF313950.

Family and domain databases

InterProiIPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9JIM1-1) [UniParc]FASTAAdd to Basket

Also known as: Ent1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFMTATKYFT NRLDVSQNVS    50
SDTDQSCEST KALADPTVAL PARSSLSAIF NNVMTLCAML PLLVFTCLNS 100
FLHQRISQSV RILGSLLAIL LVFLVTAALV KVEMDALIFF VITMIKIVLI 150
NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ GLAGFFTSVA MICAIASGSE 200
LSESAFGYFI TACAVVILAI LCYLALPRTE FYRHYLQLNL AGPAEQETKL 250
DLISKGEEPK GRREESGVPG PNSPPTNRNQ SIKAILKSIC VPALSVCFIF 300
TVTIGLFPAV TAEVESSIAG TSPWKSYFIP VACFLNFNVF DWLGRSLTAV 350
CMWPGQDSRW LPVLVASRIV FIPLLMLCNV KARHCGAQRH HFVFKHDAWF 400
IAFMAAFAFS NGYLASLCMC FGPKKVKPAE AETAGNIMSF FLCLGLALGA 450
VLSFLLRALV 460
Length:460
Mass (Da):50,192
Last modified:January 23, 2007 - v3
Checksum:iFE37EEA155F9AB2F
GO
Isoform 2 (identifier: Q9JIM1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-256: SKG → R

Show »
Length:458
Mass (Da):50,076
Checksum:iEDE0FDEF5BF3E274
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei254 – 2563SKG → R in isoform 2.
VSP_010471

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541D → G in BAB23537. 1 Publication
Sequence conflicti138 – 1381I → T in AAH04828. 1 Publication
Sequence conflicti372 – 3721I → V in AAH04828. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF218255 Genomic DNA. Translation: AAF64035.2.
AF218255 Genomic DNA. Translation: AAF64036.2.
AF305501 mRNA. Translation: AAG22828.1.
AF131212 mRNA. Translation: AAF78452.1.
AK004756 mRNA. Translation: BAB23537.1.
AK050089 mRNA. Translation: BAC34063.1.
BC004828 mRNA. Translation: AAH04828.1.
BC006812 mRNA. Translation: AAH06812.1.
CCDSiCCDS28813.1. [Q9JIM1-2]
CCDS57096.1. [Q9JIM1-1]
RefSeqiNP_001186042.1. NM_001199113.1. [Q9JIM1-1]
NP_001186043.1. NM_001199114.1. [Q9JIM1-1]
NP_001186044.1. NM_001199115.1. [Q9JIM1-2]
NP_001186045.1. NM_001199116.1. [Q9JIM1-2]
NP_075018.1. NM_022880.3. [Q9JIM1-2]
XP_006524831.1. XM_006524768.1. [Q9JIM1-1]
XP_006524832.1. XM_006524769.1. [Q9JIM1-1]
XP_006524833.1. XM_006524770.1. [Q9JIM1-1]
XP_006524834.1. XM_006524771.1. [Q9JIM1-2]
UniGeneiMm.29744.
Mm.486335.

Genome annotation databases

EnsembliENSMUST00000051574; ENSMUSP00000063096; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000064889; ENSMUSP00000063757; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000097317; ENSMUSP00000094923; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000163492; ENSMUSP00000129242; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000166119; ENSMUSP00000128763; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000167692; ENSMUSP00000131976; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000171847; ENSMUSP00000126703; ENSMUSG00000023942. [Q9JIM1-2]
GeneIDi63959.
KEGGimmu:63959.
UCSCiuc008crb.2. mouse. [Q9JIM1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF218255 Genomic DNA. Translation: AAF64035.2 .
AF218255 Genomic DNA. Translation: AAF64036.2 .
AF305501 mRNA. Translation: AAG22828.1 .
AF131212 mRNA. Translation: AAF78452.1 .
AK004756 mRNA. Translation: BAB23537.1 .
AK050089 mRNA. Translation: BAC34063.1 .
BC004828 mRNA. Translation: AAH04828.1 .
BC006812 mRNA. Translation: AAH06812.1 .
CCDSi CCDS28813.1. [Q9JIM1-2 ]
CCDS57096.1. [Q9JIM1-1 ]
RefSeqi NP_001186042.1. NM_001199113.1. [Q9JIM1-1 ]
NP_001186043.1. NM_001199114.1. [Q9JIM1-1 ]
NP_001186044.1. NM_001199115.1. [Q9JIM1-2 ]
NP_001186045.1. NM_001199116.1. [Q9JIM1-2 ]
NP_075018.1. NM_022880.3. [Q9JIM1-2 ]
XP_006524831.1. XM_006524768.1. [Q9JIM1-1 ]
XP_006524832.1. XM_006524769.1. [Q9JIM1-1 ]
XP_006524833.1. XM_006524770.1. [Q9JIM1-1 ]
XP_006524834.1. XM_006524771.1. [Q9JIM1-2 ]
UniGenei Mm.29744.
Mm.486335.

3D structure databases

ProteinModelPortali Q9JIM1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000063096.

Chemistry

ChEMBLi CHEMBL1287611.

PTM databases

PhosphoSitei Q9JIM1.

Proteomic databases

PaxDbi Q9JIM1.
PRIDEi Q9JIM1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000051574 ; ENSMUSP00000063096 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
ENSMUST00000064889 ; ENSMUSP00000063757 ; ENSMUSG00000023942 . [Q9JIM1-2 ]
ENSMUST00000097317 ; ENSMUSP00000094923 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
ENSMUST00000163492 ; ENSMUSP00000129242 ; ENSMUSG00000023942 . [Q9JIM1-2 ]
ENSMUST00000166119 ; ENSMUSP00000128763 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
ENSMUST00000167692 ; ENSMUSP00000131976 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
ENSMUST00000171847 ; ENSMUSP00000126703 ; ENSMUSG00000023942 . [Q9JIM1-2 ]
GeneIDi 63959.
KEGGi mmu:63959.
UCSCi uc008crb.2. mouse. [Q9JIM1-1 ]

Organism-specific databases

CTDi 2030.
MGIi MGI:1927073. Slc29a1.

Phylogenomic databases

eggNOGi NOG249415.
GeneTreei ENSGT00390000002232.
HOGENOMi HOG000007684.
HOVERGENi HBG074626.
InParanoidi Q9JIM1.
KOi K15014.
OMAi RIANWNI.
OrthoDBi EOG7PP56W.
PhylomeDBi Q9JIM1.
TreeFami TF313950.

Miscellaneous databases

NextBioi 319829.
PROi Q9JIM1.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9JIM1.
Bgeei Q9JIM1.
Genevestigatori Q9JIM1.

Family and domain databases

InterProi IPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view ]
PANTHERi PTHR10332. PTHR10332. 1 hit.
Pfami PF01733. Nucleoside_tran. 1 hit.
[Graphical view ]
PIRSFi PIRSF016379. ENT. 1 hit.
PRINTSi PR01130. DERENTRNSPRT.
SUPFAMi SSF103473. SSF103473. 3 hits.
TIGRFAMsi TIGR00939. 2a57. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization and expression of the mouse equilibrative, nitrobenzylthioinosine-sensitive nucleoside transporter 1 (ENT1) gene."
    Choi D.-S., Handa M., Young H., Gordon A.S., Diamond I., Messing R.O.
    Biochem. Biophys. Res. Commun. 277:200-208(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  2. "Molecular cloning and functional characterization of inhibitor-sensitive (mENT1) and inhibitor-resistant (mENT2) equilibrative nucleoside transporters from mouse brain."
    Kiss A., Farah K., Kim J., Garriock R.J., Drysdale T.A., Hammond J.R.
    Biochem. J. 352:363-372(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
    Strain: CD-1.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Liver and Lung.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Mammary tumor.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-48.

Entry informationi

Entry nameiS29A1_MOUSE
AccessioniPrimary (citable) accession number: Q9JIM1
Secondary accession number(s): Q99K84, Q9DBT8, Q9JHF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi