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Protein

Equilibrative nucleoside transporter 1

Gene

Slc29a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).

GO - Molecular functioni

  • nucleoside transmembrane transporter activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_339322. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 1
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
Short name:
Equilibrative NBMPR-sensitive nucleoside transporter
Nucleoside transporter, es-type
Solute carrier family 29 member 1
Gene namesi
Name:Slc29a1
Synonyms:Ent1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1927073. Slc29a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1211CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 2917HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 8253ExtracellularSequence AnalysisAdd
BLAST
Transmembranei83 – 10725HelicalSequence AnalysisAdd
BLAST
Topological domaini108 – 1114CytoplasmicSequence Analysis
Transmembranei112 – 13019HelicalSequence AnalysisAdd
BLAST
Topological domaini131 – 1388ExtracellularSequence Analysis
Transmembranei139 – 15719HelicalSequence AnalysisAdd
BLAST
Topological domaini158 – 17417CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei175 – 19925HelicalSequence AnalysisAdd
BLAST
Topological domaini200 – 2067ExtracellularSequence Analysis
Transmembranei207 – 22721HelicalSequence AnalysisAdd
BLAST
Topological domaini228 – 29164CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei292 – 31120HelicalSequence AnalysisAdd
BLAST
Topological domaini312 – 32312ExtracellularSequence AnalysisAdd
BLAST
Transmembranei324 – 34219HelicalSequence AnalysisAdd
BLAST
Topological domaini343 – 35917CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei360 – 37819HelicalSequence AnalysisAdd
BLAST
Topological domaini379 – 39719ExtracellularSequence AnalysisAdd
BLAST
Transmembranei398 – 41720HelicalSequence AnalysisAdd
BLAST
Topological domaini418 – 43518CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei436 – 45621HelicalSequence AnalysisAdd
BLAST
Topological domaini457 – 4604ExtracellularSequence Analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 460459Equilibrative nucleoside transporter 1PRO_0000209338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi48 – 481N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9JIM1.
PaxDbiQ9JIM1.
PRIDEiQ9JIM1.

PTM databases

PhosphoSiteiQ9JIM1.

Expressioni

Tissue specificityi

Highly expressed in heart, spleen, lung, liver and testis. Lower level of expression in brain and kidney.1 Publication

Gene expression databases

BgeeiQ9JIM1.
ExpressionAtlasiQ9JIM1. baseline and differential.
GenevisibleiQ9JIM1. MM.

Interactioni

Subunit structurei

Identified in a complex with STOM.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063096.

Structurei

3D structure databases

ProteinModelPortaliQ9JIM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiQ9JIM1.
KOiK15014.
OMAiLINXISV.
OrthoDBiEOG7PP56W.
PhylomeDBiQ9JIM1.
TreeFamiTF313950.

Family and domain databases

InterProiIPR030195. ENT1.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF9. PTHR10332:SF9. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JIM1-1) [UniParc]FASTAAdd to basket

Also known as: Ent1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFMTATKYFT NRLDVSQNVS
60 70 80 90 100
SDTDQSCEST KALADPTVAL PARSSLSAIF NNVMTLCAML PLLVFTCLNS
110 120 130 140 150
FLHQRISQSV RILGSLLAIL LVFLVTAALV KVEMDALIFF VITMIKIVLI
160 170 180 190 200
NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ GLAGFFTSVA MICAIASGSE
210 220 230 240 250
LSESAFGYFI TACAVVILAI LCYLALPRTE FYRHYLQLNL AGPAEQETKL
260 270 280 290 300
DLISKGEEPK GRREESGVPG PNSPPTNRNQ SIKAILKSIC VPALSVCFIF
310 320 330 340 350
TVTIGLFPAV TAEVESSIAG TSPWKSYFIP VACFLNFNVF DWLGRSLTAV
360 370 380 390 400
CMWPGQDSRW LPVLVASRIV FIPLLMLCNV KARHCGAQRH HFVFKHDAWF
410 420 430 440 450
IAFMAAFAFS NGYLASLCMC FGPKKVKPAE AETAGNIMSF FLCLGLALGA
460
VLSFLLRALV
Length:460
Mass (Da):50,192
Last modified:January 23, 2007 - v3
Checksum:iFE37EEA155F9AB2F
GO
Isoform 2 (identifier: Q9JIM1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-256: SKG → R

Show »
Length:458
Mass (Da):50,076
Checksum:iEDE0FDEF5BF3E274
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541D → G in BAB23537 (PubMed:16141072).Curated
Sequence conflicti138 – 1381I → T in AAH04828 (PubMed:15489334).Curated
Sequence conflicti372 – 3721I → V in AAH04828 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei254 – 2563SKG → R in isoform 2. 3 PublicationsVSP_010471

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218255 Genomic DNA. Translation: AAF64035.2.
AF218255 Genomic DNA. Translation: AAF64036.2.
AF305501 mRNA. Translation: AAG22828.1.
AF131212 mRNA. Translation: AAF78452.1.
AK004756 mRNA. Translation: BAB23537.1.
AK050089 mRNA. Translation: BAC34063.1.
BC004828 mRNA. Translation: AAH04828.1.
BC006812 mRNA. Translation: AAH06812.1.
CCDSiCCDS28813.1. [Q9JIM1-2]
CCDS57096.1. [Q9JIM1-1]
RefSeqiNP_001186042.1. NM_001199113.1. [Q9JIM1-1]
NP_001186043.1. NM_001199114.1. [Q9JIM1-1]
NP_001186044.1. NM_001199115.1. [Q9JIM1-2]
NP_001186045.1. NM_001199116.1. [Q9JIM1-2]
NP_075018.1. NM_022880.3. [Q9JIM1-2]
XP_006524831.1. XM_006524768.1. [Q9JIM1-1]
XP_006524832.1. XM_006524769.2. [Q9JIM1-1]
XP_006524833.1. XM_006524770.2. [Q9JIM1-1]
XP_006524834.1. XM_006524771.1. [Q9JIM1-2]
UniGeneiMm.29744.
Mm.486335.

Genome annotation databases

EnsembliENSMUST00000051574; ENSMUSP00000063096; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000064889; ENSMUSP00000063757; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000097317; ENSMUSP00000094923; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000163492; ENSMUSP00000129242; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000166119; ENSMUSP00000128763; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000167692; ENSMUSP00000131976; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000171847; ENSMUSP00000126703; ENSMUSG00000023942. [Q9JIM1-2]
GeneIDi63959.
KEGGimmu:63959.
UCSCiuc008crb.2. mouse. [Q9JIM1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218255 Genomic DNA. Translation: AAF64035.2.
AF218255 Genomic DNA. Translation: AAF64036.2.
AF305501 mRNA. Translation: AAG22828.1.
AF131212 mRNA. Translation: AAF78452.1.
AK004756 mRNA. Translation: BAB23537.1.
AK050089 mRNA. Translation: BAC34063.1.
BC004828 mRNA. Translation: AAH04828.1.
BC006812 mRNA. Translation: AAH06812.1.
CCDSiCCDS28813.1. [Q9JIM1-2]
CCDS57096.1. [Q9JIM1-1]
RefSeqiNP_001186042.1. NM_001199113.1. [Q9JIM1-1]
NP_001186043.1. NM_001199114.1. [Q9JIM1-1]
NP_001186044.1. NM_001199115.1. [Q9JIM1-2]
NP_001186045.1. NM_001199116.1. [Q9JIM1-2]
NP_075018.1. NM_022880.3. [Q9JIM1-2]
XP_006524831.1. XM_006524768.1. [Q9JIM1-1]
XP_006524832.1. XM_006524769.2. [Q9JIM1-1]
XP_006524833.1. XM_006524770.2. [Q9JIM1-1]
XP_006524834.1. XM_006524771.1. [Q9JIM1-2]
UniGeneiMm.29744.
Mm.486335.

3D structure databases

ProteinModelPortaliQ9JIM1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063096.

Chemistry

ChEMBLiCHEMBL1287611.

PTM databases

PhosphoSiteiQ9JIM1.

Proteomic databases

MaxQBiQ9JIM1.
PaxDbiQ9JIM1.
PRIDEiQ9JIM1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051574; ENSMUSP00000063096; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000064889; ENSMUSP00000063757; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000097317; ENSMUSP00000094923; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000163492; ENSMUSP00000129242; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000166119; ENSMUSP00000128763; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000167692; ENSMUSP00000131976; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000171847; ENSMUSP00000126703; ENSMUSG00000023942. [Q9JIM1-2]
GeneIDi63959.
KEGGimmu:63959.
UCSCiuc008crb.2. mouse. [Q9JIM1-1]

Organism-specific databases

CTDi2030.
MGIiMGI:1927073. Slc29a1.

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiQ9JIM1.
KOiK15014.
OMAiLINXISV.
OrthoDBiEOG7PP56W.
PhylomeDBiQ9JIM1.
TreeFamiTF313950.

Enzyme and pathway databases

ReactomeiREACT_339322. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Miscellaneous databases

NextBioi319829.
PROiQ9JIM1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JIM1.
ExpressionAtlasiQ9JIM1. baseline and differential.
GenevisibleiQ9JIM1. MM.

Family and domain databases

InterProiIPR030195. ENT1.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF9. PTHR10332:SF9. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization and expression of the mouse equilibrative, nitrobenzylthioinosine-sensitive nucleoside transporter 1 (ENT1) gene."
    Choi D.-S., Handa M., Young H., Gordon A.S., Diamond I., Messing R.O.
    Biochem. Biophys. Res. Commun. 277:200-208(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  2. "Molecular cloning and functional characterization of inhibitor-sensitive (mENT1) and inhibitor-resistant (mENT2) equilibrative nucleoside transporters from mouse brain."
    Kiss A., Farah K., Kim J., Garriock R.J., Drysdale T.A., Hammond J.R.
    Biochem. J. 352:363-372(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
    Strain: CD-1.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Liver and Lung.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Mammary tumor.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-48.

Entry informationi

Entry nameiS29A1_MOUSE
AccessioniPrimary (citable) accession number: Q9JIM1
Secondary accession number(s): Q99K84, Q9DBT8, Q9JHF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.