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Q9JIM1

- S29A1_MOUSE

UniProt

Q9JIM1 - S29A1_MOUSE

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Protein

Equilibrative nucleoside transporter 1

Gene

Slc29a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).

GO - Molecular functioni

  1. nucleoside transmembrane transporter activity Source: MGI

GO - Biological processi

  1. cellular response to glucose stimulus Source: Ensembl
  2. cellular response to hypoxia Source: Ensembl
  3. lactation Source: Ensembl
  4. nucleoside transmembrane transport Source: GOC
  5. nucleoside transport Source: MGI
  6. regulation of excitatory postsynaptic membrane potential Source: Ensembl
  7. sleep Source: Ensembl
  8. uridine transport Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_247934. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 1
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
Short name:
Equilibrative NBMPR-sensitive nucleoside transporter
Nucleoside transporter, es-type
Solute carrier family 29 member 1
Gene namesi
Name:Slc29a1
Synonyms:Ent1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1927073. Slc29a1.

Subcellular locationi

Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity. Apical cell membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Predominantly localized in the basolateral membrane in polarized MDCK cells.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1211CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 2917HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 8253ExtracellularSequence AnalysisAdd
BLAST
Transmembranei83 – 10725HelicalSequence AnalysisAdd
BLAST
Topological domaini108 – 1114CytoplasmicSequence Analysis
Transmembranei112 – 13019HelicalSequence AnalysisAdd
BLAST
Topological domaini131 – 1388ExtracellularSequence Analysis
Transmembranei139 – 15719HelicalSequence AnalysisAdd
BLAST
Topological domaini158 – 17417CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei175 – 19925HelicalSequence AnalysisAdd
BLAST
Topological domaini200 – 2067ExtracellularSequence Analysis
Transmembranei207 – 22721HelicalSequence AnalysisAdd
BLAST
Topological domaini228 – 29164CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei292 – 31120HelicalSequence AnalysisAdd
BLAST
Topological domaini312 – 32312ExtracellularSequence AnalysisAdd
BLAST
Transmembranei324 – 34219HelicalSequence AnalysisAdd
BLAST
Topological domaini343 – 35917CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei360 – 37819HelicalSequence AnalysisAdd
BLAST
Topological domaini379 – 39719ExtracellularSequence AnalysisAdd
BLAST
Transmembranei398 – 41720HelicalSequence AnalysisAdd
BLAST
Topological domaini418 – 43518CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei436 – 45621HelicalSequence AnalysisAdd
BLAST
Topological domaini457 – 4604ExtracellularSequence Analysis

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. basolateral plasma membrane Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 460459Equilibrative nucleoside transporter 1PRO_0000209338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi48 – 481N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9JIM1.
PaxDbiQ9JIM1.
PRIDEiQ9JIM1.

PTM databases

PhosphoSiteiQ9JIM1.

Expressioni

Tissue specificityi

Highly expressed in heart, spleen, lung, liver and testis. Lower level of expression in brain and kidney.1 Publication

Gene expression databases

BgeeiQ9JIM1.
ExpressionAtlasiQ9JIM1. baseline and differential.
GenevestigatoriQ9JIM1.

Interactioni

Subunit structurei

Identified in a complex with STOM.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063096.

Structurei

3D structure databases

ProteinModelPortaliQ9JIM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiQ9JIM1.
KOiK15014.
OMAiRIANWNI.
OrthoDBiEOG7PP56W.
PhylomeDBiQ9JIM1.
TreeFamiTF313950.

Family and domain databases

InterProiIPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9JIM1-1) [UniParc]FASTAAdd to Basket

Also known as: Ent1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFMTATKYFT NRLDVSQNVS
60 70 80 90 100
SDTDQSCEST KALADPTVAL PARSSLSAIF NNVMTLCAML PLLVFTCLNS
110 120 130 140 150
FLHQRISQSV RILGSLLAIL LVFLVTAALV KVEMDALIFF VITMIKIVLI
160 170 180 190 200
NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ GLAGFFTSVA MICAIASGSE
210 220 230 240 250
LSESAFGYFI TACAVVILAI LCYLALPRTE FYRHYLQLNL AGPAEQETKL
260 270 280 290 300
DLISKGEEPK GRREESGVPG PNSPPTNRNQ SIKAILKSIC VPALSVCFIF
310 320 330 340 350
TVTIGLFPAV TAEVESSIAG TSPWKSYFIP VACFLNFNVF DWLGRSLTAV
360 370 380 390 400
CMWPGQDSRW LPVLVASRIV FIPLLMLCNV KARHCGAQRH HFVFKHDAWF
410 420 430 440 450
IAFMAAFAFS NGYLASLCMC FGPKKVKPAE AETAGNIMSF FLCLGLALGA
460
VLSFLLRALV
Length:460
Mass (Da):50,192
Last modified:January 23, 2007 - v3
Checksum:iFE37EEA155F9AB2F
GO
Isoform 2 (identifier: Q9JIM1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-256: SKG → R

Show »
Length:458
Mass (Da):50,076
Checksum:iEDE0FDEF5BF3E274
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541D → G in BAB23537. (PubMed:16141072)Curated
Sequence conflicti138 – 1381I → T in AAH04828. (PubMed:15489334)Curated
Sequence conflicti372 – 3721I → V in AAH04828. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei254 – 2563SKG → R in isoform 2. 3 PublicationsVSP_010471

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218255 Genomic DNA. Translation: AAF64035.2.
AF218255 Genomic DNA. Translation: AAF64036.2.
AF305501 mRNA. Translation: AAG22828.1.
AF131212 mRNA. Translation: AAF78452.1.
AK004756 mRNA. Translation: BAB23537.1.
AK050089 mRNA. Translation: BAC34063.1.
BC004828 mRNA. Translation: AAH04828.1.
BC006812 mRNA. Translation: AAH06812.1.
CCDSiCCDS28813.1. [Q9JIM1-2]
CCDS57096.1. [Q9JIM1-1]
RefSeqiNP_001186042.1. NM_001199113.1. [Q9JIM1-1]
NP_001186043.1. NM_001199114.1. [Q9JIM1-1]
NP_001186044.1. NM_001199115.1. [Q9JIM1-2]
NP_001186045.1. NM_001199116.1. [Q9JIM1-2]
NP_075018.1. NM_022880.3. [Q9JIM1-2]
XP_006524831.1. XM_006524768.1. [Q9JIM1-1]
XP_006524832.1. XM_006524769.1. [Q9JIM1-1]
XP_006524833.1. XM_006524770.1. [Q9JIM1-1]
XP_006524834.1. XM_006524771.1. [Q9JIM1-2]
UniGeneiMm.29744.
Mm.486335.

Genome annotation databases

EnsembliENSMUST00000051574; ENSMUSP00000063096; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000064889; ENSMUSP00000063757; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000097317; ENSMUSP00000094923; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000163492; ENSMUSP00000129242; ENSMUSG00000023942. [Q9JIM1-2]
ENSMUST00000166119; ENSMUSP00000128763; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000167692; ENSMUSP00000131976; ENSMUSG00000023942. [Q9JIM1-1]
ENSMUST00000171847; ENSMUSP00000126703; ENSMUSG00000023942. [Q9JIM1-2]
GeneIDi63959.
KEGGimmu:63959.
UCSCiuc008crb.2. mouse. [Q9JIM1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218255 Genomic DNA. Translation: AAF64035.2 .
AF218255 Genomic DNA. Translation: AAF64036.2 .
AF305501 mRNA. Translation: AAG22828.1 .
AF131212 mRNA. Translation: AAF78452.1 .
AK004756 mRNA. Translation: BAB23537.1 .
AK050089 mRNA. Translation: BAC34063.1 .
BC004828 mRNA. Translation: AAH04828.1 .
BC006812 mRNA. Translation: AAH06812.1 .
CCDSi CCDS28813.1. [Q9JIM1-2 ]
CCDS57096.1. [Q9JIM1-1 ]
RefSeqi NP_001186042.1. NM_001199113.1. [Q9JIM1-1 ]
NP_001186043.1. NM_001199114.1. [Q9JIM1-1 ]
NP_001186044.1. NM_001199115.1. [Q9JIM1-2 ]
NP_001186045.1. NM_001199116.1. [Q9JIM1-2 ]
NP_075018.1. NM_022880.3. [Q9JIM1-2 ]
XP_006524831.1. XM_006524768.1. [Q9JIM1-1 ]
XP_006524832.1. XM_006524769.1. [Q9JIM1-1 ]
XP_006524833.1. XM_006524770.1. [Q9JIM1-1 ]
XP_006524834.1. XM_006524771.1. [Q9JIM1-2 ]
UniGenei Mm.29744.
Mm.486335.

3D structure databases

ProteinModelPortali Q9JIM1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000063096.

Chemistry

ChEMBLi CHEMBL1287611.

PTM databases

PhosphoSitei Q9JIM1.

Proteomic databases

MaxQBi Q9JIM1.
PaxDbi Q9JIM1.
PRIDEi Q9JIM1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000051574 ; ENSMUSP00000063096 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
ENSMUST00000064889 ; ENSMUSP00000063757 ; ENSMUSG00000023942 . [Q9JIM1-2 ]
ENSMUST00000097317 ; ENSMUSP00000094923 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
ENSMUST00000163492 ; ENSMUSP00000129242 ; ENSMUSG00000023942 . [Q9JIM1-2 ]
ENSMUST00000166119 ; ENSMUSP00000128763 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
ENSMUST00000167692 ; ENSMUSP00000131976 ; ENSMUSG00000023942 . [Q9JIM1-1 ]
ENSMUST00000171847 ; ENSMUSP00000126703 ; ENSMUSG00000023942 . [Q9JIM1-2 ]
GeneIDi 63959.
KEGGi mmu:63959.
UCSCi uc008crb.2. mouse. [Q9JIM1-1 ]

Organism-specific databases

CTDi 2030.
MGIi MGI:1927073. Slc29a1.

Phylogenomic databases

eggNOGi NOG249415.
GeneTreei ENSGT00390000002232.
HOGENOMi HOG000007684.
HOVERGENi HBG074626.
InParanoidi Q9JIM1.
KOi K15014.
OMAi RIANWNI.
OrthoDBi EOG7PP56W.
PhylomeDBi Q9JIM1.
TreeFami TF313950.

Enzyme and pathway databases

Reactomei REACT_247934. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Miscellaneous databases

NextBioi 319829.
PROi Q9JIM1.
SOURCEi Search...

Gene expression databases

Bgeei Q9JIM1.
ExpressionAtlasi Q9JIM1. baseline and differential.
Genevestigatori Q9JIM1.

Family and domain databases

InterProi IPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view ]
PANTHERi PTHR10332. PTHR10332. 1 hit.
Pfami PF01733. Nucleoside_tran. 1 hit.
[Graphical view ]
PIRSFi PIRSF016379. ENT. 1 hit.
PRINTSi PR01130. DERENTRNSPRT.
SUPFAMi SSF103473. SSF103473. 3 hits.
TIGRFAMsi TIGR00939. 2a57. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization and expression of the mouse equilibrative, nitrobenzylthioinosine-sensitive nucleoside transporter 1 (ENT1) gene."
    Choi D.-S., Handa M., Young H., Gordon A.S., Diamond I., Messing R.O.
    Biochem. Biophys. Res. Commun. 277:200-208(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  2. "Molecular cloning and functional characterization of inhibitor-sensitive (mENT1) and inhibitor-resistant (mENT2) equilibrative nucleoside transporters from mouse brain."
    Kiss A., Farah K., Kim J., Garriock R.J., Drysdale T.A., Hammond J.R.
    Biochem. J. 352:363-372(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
    Strain: CD-1.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Liver and Lung.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Mammary tumor.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-48.

Entry informationi

Entry nameiS29A1_MOUSE
AccessioniPrimary (citable) accession number: Q9JIM1
Secondary accession number(s): Q99K84, Q9DBT8, Q9JHF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3