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Protein

Equilibrative nucleoside transporter 1

Gene

Slc29a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).

GO - Molecular functioni

  • nucleoside transmembrane transporter activity Source: MGI

GO - Biological processi

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-MMU-83936 Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 1
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
Short name:
Equilibrative NBMPR-sensitive nucleoside transporter
Nucleoside transporter, es-type
Solute carrier family 29 member 1
Gene namesi
Name:Slc29a1
Synonyms:Ent1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1927073 Slc29a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 29HelicalSequence analysisAdd BLAST17
Topological domaini30 – 82ExtracellularSequence analysisAdd BLAST53
Transmembranei83 – 107HelicalSequence analysisAdd BLAST25
Topological domaini108 – 111CytoplasmicSequence analysis4
Transmembranei112 – 130HelicalSequence analysisAdd BLAST19
Topological domaini131 – 138ExtracellularSequence analysis8
Transmembranei139 – 157HelicalSequence analysisAdd BLAST19
Topological domaini158 – 174CytoplasmicSequence analysisAdd BLAST17
Transmembranei175 – 199HelicalSequence analysisAdd BLAST25
Topological domaini200 – 206ExtracellularSequence analysis7
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 291CytoplasmicSequence analysisAdd BLAST64
Transmembranei292 – 311HelicalSequence analysisAdd BLAST20
Topological domaini312 – 323ExtracellularSequence analysisAdd BLAST12
Transmembranei324 – 342HelicalSequence analysisAdd BLAST19
Topological domaini343 – 359CytoplasmicSequence analysisAdd BLAST17
Transmembranei360 – 378HelicalSequence analysisAdd BLAST19
Topological domaini379 – 397ExtracellularSequence analysisAdd BLAST19
Transmembranei398 – 417HelicalSequence analysisAdd BLAST20
Topological domaini418 – 435CytoplasmicSequence analysisAdd BLAST18
Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
Topological domaini457 – 460ExtracellularSequence analysis4

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1287611

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002093381 – 460Equilibrative nucleoside transporter 1Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi48N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei254PhosphoserineBy similarity1
Modified residuei273PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9JIM1
PaxDbiQ9JIM1
PRIDEiQ9JIM1

PTM databases

iPTMnetiQ9JIM1
PhosphoSitePlusiQ9JIM1
SwissPalmiQ9JIM1

Expressioni

Tissue specificityi

Highly expressed in heart, spleen, lung, liver and testis. Lower level of expression in brain and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000023942
ExpressionAtlasiQ9JIM1 baseline and differential
GenevisibleiQ9JIM1 MM

Interactioni

Subunit structurei

Identified in a complex with STOM.By similarity

Protein-protein interaction databases

BioGridi211010, 1 interactor
STRINGi10090.ENSMUSP00000063096

Structurei

3D structure databases

ProteinModelPortaliQ9JIM1
SMRiQ9JIM1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1479 Eukaryota
ENOG410Y3MT LUCA
GeneTreeiENSGT00390000002232
HOGENOMiHOG000007684
HOVERGENiHBG074626
InParanoidiQ9JIM1
KOiK15014
OMAiFNIFDWL
OrthoDBiEOG091G09WB
PhylomeDBiQ9JIM1
TreeFamiTF313950

Family and domain databases

InterProiView protein in InterPro
IPR030195 ENT1
IPR034764 ENT1/ENT2
IPR002259 Eqnu_transpt
IPR036259 MFS_trans_sf
PANTHERiPTHR10332 PTHR10332, 1 hit
PTHR10332:SF9 PTHR10332:SF9, 1 hit
PfamiView protein in Pfam
PF01733 Nucleoside_tran, 1 hit
PIRSFiPIRSF016379 ENT, 1 hit
PRINTSiPR01130 DERENTRNSPRT
SUPFAMiSSF103473 SSF103473, 3 hits
TIGRFAMsiTIGR00939 2a57, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JIM1-1) [UniParc]FASTAAdd to basket
Also known as: Ent1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFMTATKYFT NRLDVSQNVS
60 70 80 90 100
SDTDQSCEST KALADPTVAL PARSSLSAIF NNVMTLCAML PLLVFTCLNS
110 120 130 140 150
FLHQRISQSV RILGSLLAIL LVFLVTAALV KVEMDALIFF VITMIKIVLI
160 170 180 190 200
NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ GLAGFFTSVA MICAIASGSE
210 220 230 240 250
LSESAFGYFI TACAVVILAI LCYLALPRTE FYRHYLQLNL AGPAEQETKL
260 270 280 290 300
DLISKGEEPK GRREESGVPG PNSPPTNRNQ SIKAILKSIC VPALSVCFIF
310 320 330 340 350
TVTIGLFPAV TAEVESSIAG TSPWKSYFIP VACFLNFNVF DWLGRSLTAV
360 370 380 390 400
CMWPGQDSRW LPVLVASRIV FIPLLMLCNV KARHCGAQRH HFVFKHDAWF
410 420 430 440 450
IAFMAAFAFS NGYLASLCMC FGPKKVKPAE AETAGNIMSF FLCLGLALGA
460
VLSFLLRALV
Length:460
Mass (Da):50,192
Last modified:January 23, 2007 - v3
Checksum:iFE37EEA155F9AB2F
GO
Isoform 2 (identifier: Q9JIM1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-256: SKG → R

Show »
Length:458
Mass (Da):50,076
Checksum:iEDE0FDEF5BF3E274
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54D → G in BAB23537 (PubMed:16141072).Curated1
Sequence conflicti138I → T in AAH04828 (PubMed:15489334).Curated1
Sequence conflicti372I → V in AAH04828 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010471254 – 256SKG → R in isoform 2. 3 Publications3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218255 Genomic DNA Translation: AAF64035.2
AF218255 Genomic DNA Translation: AAF64036.2
AF305501 mRNA Translation: AAG22828.1
AF131212 mRNA Translation: AAF78452.1
AK004756 mRNA Translation: BAB23537.1
AK050089 mRNA Translation: BAC34063.1
BC004828 mRNA Translation: AAH04828.1
BC006812 mRNA Translation: AAH06812.1
CCDSiCCDS28813.1 [Q9JIM1-2]
CCDS57096.1 [Q9JIM1-1]
RefSeqiNP_001186042.1, NM_001199113.1 [Q9JIM1-1]
NP_001186043.1, NM_001199114.1 [Q9JIM1-1]
NP_001186044.1, NM_001199115.1 [Q9JIM1-2]
NP_001186045.1, NM_001199116.1 [Q9JIM1-2]
NP_075018.1, NM_022880.3 [Q9JIM1-2]
XP_006524831.1, XM_006524768.1 [Q9JIM1-1]
XP_006524832.1, XM_006524769.2 [Q9JIM1-1]
XP_006524833.1, XM_006524770.3 [Q9JIM1-1]
XP_006524834.1, XM_006524771.1
UniGeneiMm.29744
Mm.486335

Genome annotation databases

EnsembliENSMUST00000051574; ENSMUSP00000063096; ENSMUSG00000023942 [Q9JIM1-1]
ENSMUST00000064889; ENSMUSP00000063757; ENSMUSG00000023942 [Q9JIM1-2]
ENSMUST00000097317; ENSMUSP00000094923; ENSMUSG00000023942 [Q9JIM1-1]
ENSMUST00000163492; ENSMUSP00000129242; ENSMUSG00000023942 [Q9JIM1-2]
ENSMUST00000166119; ENSMUSP00000128763; ENSMUSG00000023942 [Q9JIM1-1]
ENSMUST00000167692; ENSMUSP00000131976; ENSMUSG00000023942 [Q9JIM1-1]
ENSMUST00000171847; ENSMUSP00000126703; ENSMUSG00000023942 [Q9JIM1-2]
GeneIDi63959
KEGGimmu:63959
UCSCiuc008crb.2 mouse [Q9JIM1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiS29A1_MOUSE
AccessioniPrimary (citable) accession number: Q9JIM1
Secondary accession number(s): Q99K84, Q9DBT8, Q9JHF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 128 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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