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Protein

DNA repair protein RAD50

Gene

Rad50

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per homodimer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi681 – 6811ZincPROSITE-ProRule annotation
Metal bindingi684 – 6841ZincPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 438ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: RGD
  • chromosome organization involved in meiotic cell cycle Source: GO_Central
  • DNA duplex unwinding Source: GO_Central
  • DNA recombination Source: UniProtKB
  • DNA repair Source: UniProtKB
  • double-strand break repair Source: UniProtKB
  • double-strand break repair via homologous recombination Source: GO_Central
  • heart development Source: RGD
  • negative regulation of viral entry into host cell Source: RGD
  • nucleic acid phosphodiester bond hydrolysis Source: GO_Central
  • protein heterooligomerization Source: RGD
  • reciprocal meiotic recombination Source: RGD
  • regulation of mitotic recombination Source: UniProtKB
  • response to human chorionic gonadotropin Source: RGD
  • telomere capping Source: GO_Central
  • telomere maintenance Source: RGD
  • telomere maintenance via recombination Source: GO_Central
  • telomere maintenance via telomerase Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, Meiosis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD50 (EC:3.6.-.-)
Gene namesi
Name:Rad50
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621542. Rad50.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome Source: RGD
  • inclusion body Source: RGD
  • Mre11 complex Source: UniProtKB
  • nuclear chromatin Source: RGD
  • nuclear chromosome, telomeric region Source: GO_Central
  • nucleoplasm Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • site of double-strand break Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13121312DNA repair protein RAD50PRO_0000138643Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei635 – 6351PhosphoserineBy similarity
Modified residuei690 – 6901PhosphothreonineBy similarity
Modified residuei959 – 9591N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9JIL8.
PRIDEiQ9JIL8.

PTM databases

iPTMnetiQ9JIL8.

Expressioni

Tissue specificityi

Present at low levels in the heart at fetal-day 17, at relatively constant levels at postnatal days 10, 17 and 21 and at slightly lower levels in the adult heart. Detected in liver, kidney and lung. Barely detectable in skeletal muscle with slightly higher levels observed in brain and the ventricles of the heart (at protein level).1 Publication

Interactioni

Subunit structurei

Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248931. 1 interaction.
STRINGi10116.ENSRNOP00000062378.

Structurei

3D structure databases

ProteinModelPortaliQ9JIL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini635 – 734100Zinc-hookPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili200 – 534335Sequence analysisAdd
BLAST
Coiled coili635 – 67339Sequence analysisAdd
BLAST
Coiled coili706 – 73429Sequence analysisAdd
BLAST
Coiled coili754 – 954201Sequence analysisAdd
BLAST
Coiled coili1019 – 107557Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1201 – 123838Ala/Asp-rich (DA-box)Add
BLAST

Domaini

The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer (By similarity).By similarity

Sequence similaritiesi

Belongs to the SMC family. RAD50 subfamily.Curated
Contains 1 zinc-hook domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0962. Eukaryota.
COG0419. LUCA.
HOGENOMiHOG000090195.
HOVERGENiHBG058033.
InParanoidiQ9JIL8.
KOiK10866.
PhylomeDBiQ9JIL8.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
IPR004584. Rad50_eukaryotes.
IPR013134. Zn_hook_RAD50.
[Graphical view]
PfamiPF04423. Rad50_zn_hook. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00606. rad50. 1 hit.
PROSITEiPS51131. ZN_HOOK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRIEKMSTL GVRSFGIEDK DKQIISFFSP LTILVGPNGA GKTTIIECLK
60 70 80 90 100
YICTGDFPPG TKGNTFVHDP KVAQETDVRA QIRLQFRDVN GEMVLVQRSM
110 120 130 140 150
LCSQKSKKTE FKTLEGVITR IKHGEKVSLS SKCAEIDREM ISCLGVSKSV
160 170 180 190 200
LNNVIFCHQE DSNWPLSEGK ALKQKFDEIF SATRYIKALD TLRQVRQTQG
210 220 230 240 250
QKVKECQTEL KYLRQNKEKA CEIRDQITSK EAQLASSREI VKAYENELEP
260 270 280 290 300
LKNRLKEIEH NLSKIMRLDN EIKALDSRKK QMEKDNSELE QKMEKVFQGT
310 320 330 340 350
DEQLNDLYHN HQRTVREKER RLVDCQRELE KLSKEARLLN QERAELLVEQ
360 370 380 390 400
GRLQLQADRH QEHIRARDSL IQSLAAHLEL DGFERGPFSE RQIKNFHELV
410 420 430 440 450
RERQEREAKT ASQLLSDLTD KEALKQRQMD EMRDKKSGLG RMIELKTEIL
460 470 480 490 500
TKKQTELRNV RNELQQLEGS SDRILELDQE LTKAERELSK AEKNSSIETL
510 520 530 540 550
KAEILNLQSE KADLDRNLRK LDQEMEQLNH HTTTRTQMEM LTKDKTDKDE
560 570 580 590 600
QIRKIKSRHS DELTSLLGYF PNKKQLEDWL HSKSKEINQT RDRLAKLNKE
610 620 630 640 650
LASAEQNKNH INNELKKKEE QLSSYEDKLF DVCGSQDFES DLDRLKEDIE
660 670 680 690 700
KSSKQRAMLA GATAVYSQFI TQLTDENQSC CPGCQRVFQT EAELQEVISD
710 720 730 740 750
LQSKLRLAPD KLKSTESELK KKERRRDEML GLVPMRQSII DLKEKEIPEL
760 770 780 790 800
RNRLQSVNRD IQRLKNDIEE QETLLGTVMP EEESAKVCLT DVTIMERFQM
810 820 830 840 850
ELKDVERKIA QQAAKLQGVD LDRTVQQVNQ EKQEKQHKLD TVSSKIELNR
860 870 880 890 900
KLIQDQQEQI QHLKSKTNEL KSEKLQIATN LQRRQQMEEQ TVELSTEVQS
910 920 930 940 950
LNREIKDAKE QINPLEIALE KLQQEKEELI HRKNTSNKMA QDKINDIKEK
960 970 980 990 1000
VKNIHGYMKD IENYIQDGKD DYKKQKETEL NEVVIQLNEC DKHKEKINKE
1010 1020 1030 1040 1050
MGTMRQDIDT KKIQERWLQD NLTLRKRREE LKEVEEERKQ HLKEMGQMQV
1060 1070 1080 1090 1100
LQMKNEHQKL EENIDTIKRN HSLALGRQKG YEEEILHFKK ELREPQFRDA
1110 1120 1130 1140 1150
EEKYREMMIV MRTTELVNKD LDIYYKTLDH AIMKFHSMKM EEINKIIRDL
1160 1170 1180 1190 1200
WRSTYRGQDI EYIEIRSDAD ENVSASDKRR NYNYRVVMLK GDTALDMRGR
1210 1220 1230 1240 1250
CSAGQKVLAS LIIRLALAET FCLNCGILAL DEPTTNLDRE NIESLAHALV
1260 1270 1280 1290 1300
EIIKSRSQQR NFQLLVITHD EDFVELLGRS EYVEKFYRVK KNIDQCSEIV
1310
KSSINSLGSY VH
Length:1,312
Mass (Da):153,784
Last modified:October 1, 2000 - v1
Checksum:iF13C041BD2C05932
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218576 mRNA. Translation: AAF91229.1.
RefSeqiNP_071582.1. NM_022246.1.
UniGeneiRn.51136.

Genome annotation databases

GeneIDi64012.
KEGGirno:64012.
UCSCiRGD:621542. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218576 mRNA. Translation: AAF91229.1.
RefSeqiNP_071582.1. NM_022246.1.
UniGeneiRn.51136.

3D structure databases

ProteinModelPortaliQ9JIL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248931. 1 interaction.
STRINGi10116.ENSRNOP00000062378.

PTM databases

iPTMnetiQ9JIL8.

Proteomic databases

PaxDbiQ9JIL8.
PRIDEiQ9JIL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64012.
KEGGirno:64012.
UCSCiRGD:621542. rat.

Organism-specific databases

CTDi10111.
RGDi621542. Rad50.

Phylogenomic databases

eggNOGiKOG0962. Eukaryota.
COG0419. LUCA.
HOGENOMiHOG000090195.
HOVERGENiHBG058033.
InParanoidiQ9JIL8.
KOiK10866.
PhylomeDBiQ9JIL8.

Miscellaneous databases

PROiQ9JIL8.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
IPR004584. Rad50_eukaryotes.
IPR013134. Zn_hook_RAD50.
[Graphical view]
PfamiPF04423. Rad50_zn_hook. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00606. rad50. 1 hit.
PROSITEiPS51131. ZN_HOOK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The MRE11-NBS1-RAD50 pathway is perturbed in SV40 large T antigen-immortalized AT-1, AT-2 and HL-1 cardiomyocytes."
    Lanson N.A. Jr., Egeland D.B., Royals B.A., Claycomb W.C.
    Nucleic Acids Res. 28:2882-2892(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 454-458; 726-736; 824-832 AND 1127-1134, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiRAD50_RAT
AccessioniPrimary (citable) accession number: Q9JIL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.