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Q9JIL3

- ILF3_RAT

UniProt

Q9JIL3 - ILF3_RAT

Protein

Interleukin enhancer-binding factor 3

Gene

Ilf3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (12 Apr 2005)
      Previous versions | rss
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    Functioni

    May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA By similarity. Can regulate protein arginine N-methyltransferase 1 activity. The phosphorylated form at Thr-188 and Thr-315, in concert with EIF2AK2/PKR can inhibit vesicular stomatitis virus (VSV) replication By similarity.By similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. enzyme binding Source: RGD
    3. protein binding Source: IntAct
    4. RNA binding Source: UniProtKB-KW
    5. sequence-specific DNA binding transcription factor activity Source: RGD

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW
    2. negative regulation of translation Source: UniProtKB
    3. negative regulation of viral genome replication Source: UniProtKB
    4. protein methylation Source: MGI
    5. protein phosphorylation Source: UniProtKB
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Antiviral defense, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interleukin enhancer-binding factor 3
    Gene namesi
    Name:Ilf3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi619734. Ilf3.

    Subcellular locationi

    Nucleusnucleolus By similarity. Cytoplasm By similarity. Nucleus By similarity
    Note: TThe unphosphorylated form is retained in the nucleus by ILF2. Phosphorylation at Thr-188 and Thr-315 causes the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3. Localized in cytoplasmic mRNP granules containing untranslated mRNAs By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleolus Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB
    4. ribonucleoprotein complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 897897Interleukin enhancer-binding factor 3PRO_0000126072Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei100 – 1001N6-acetyllysineBy similarity
    Modified residuei188 – 1881Phosphothreonine; by PKRBy similarity
    Modified residuei190 – 1901PhosphoserineBy similarity
    Modified residuei315 – 3151Phosphothreonine; by PKRBy similarity
    Modified residuei382 – 3821PhosphoserineBy similarity
    Modified residuei384 – 3841PhosphoserineBy similarity
    Modified residuei460 – 4601N6-acetyllysineBy similarity
    Modified residuei476 – 4761PhosphoserineBy similarity
    Modified residuei482 – 4821PhosphoserineBy similarity
    Modified residuei609 – 6091Omega-N-methylated arginineBy similarity
    Modified residuei794 – 7941PhosphoserineBy similarity
    Modified residuei814 – 8141PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated at Thr-188 and Thr-315 by PKR in response to RNA viruses. This phosphorylation results in the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3 where it can bind to viral RNAs and impede viral replication By similarity.By similarity
    Methylated by protein arginine N-methyltransferase 1.

    Keywords - PTMi

    Acetylation, Methylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9JIL3.
    PRIDEiQ9JIL3.

    PTM databases

    PhosphoSiteiQ9JIL3.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9JIL3.

    Interactioni

    Subunit structurei

    Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with FUS and SMN proteins and with PRMT1. Forms a complex with ILF2. Can also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 and the heterodimeric complex of XRCC6/KU70 and XRCC5/KU80. Forms a heteromeric complex with ZNF346 and ILF3. Found in a nuclear export complex with XPO5, ILF3, Ran and double-stranded RNA or double-stranded minihelix VA1 RNA. Found in a nuclear export complex with XPO5, RAN, ILF3, ZNF346 and double-stranded RNA. Interacts with XPO5 and ZNF346 By similarity. Forms a complex with ILF2, YLPM1, KHDRBS1, RBMX, NCOA5 and PPP1CA. Interacts with AGO1 and AGO2 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Prmt1Q630092EBI-78714,EBI-78708

    Protein-protein interaction databases

    BioGridi249972. 1 interaction.
    IntActiQ9JIL3. 1 interaction.
    STRINGi10116.ENSRNOP00000062871.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JIL3.
    SMRiQ9JIL3. Positions 403-470, 525-589.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 378374DZFAdd
    BLAST
    Domaini398 – 46770DRBM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini524 – 59067DRBM 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni609 – 897289Interaction with PRMT1Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi371 – 38919Bipartite nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi385 – 3895Poly-Lys
    Compositional biasi634 – 6374Poly-Pro
    Compositional biasi640 – 65920Arg/Gly-richAdd
    BLAST
    Compositional biasi701 – 71010Poly-Gly
    Compositional biasi796 – 8005Poly-Gly

    Sequence similaritiesi

    Contains 2 DRBM (double-stranded RNA-binding) domains.PROSITE-ProRule annotation
    Contains 1 DZF domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG307678.
    HOGENOMiHOG000232164.
    HOVERGENiHBG069915.
    InParanoidiQ9JIL3.
    KOiK13090.
    PhylomeDBiQ9JIL3.

    Family and domain databases

    Gene3Di3.30.160.20. 2 hits.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR006561. DZF.
    [Graphical view]
    PfamiPF00035. dsrm. 2 hits.
    PF07528. DZF. 1 hit.
    [Graphical view]
    SMARTiSM00358. DSRM. 2 hits.
    SM00572. DZF. 1 hit.
    [Graphical view]
    PROSITEiPS50137. DS_RBD. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JIL3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRPMRIFVND DRHVMAKHSS VYPTQEELEA VQNMVSHTER ALKAVSDWID    50
    EQEKGNSELS EAENMDTPPD DESKEGAGEQ KAEHMTRTLR GVMRVGLVAK 100
    GLLLKGDLDL ELVLLCKEKP TTALLDKVAD NLAIQLTTVT EDKYEILQSV 150
    DDAAIVIKNT KEPPLSLTIH LTSPVVREEM EKVLAGETLS VNDPPDVLDR 200
    QKCLAALASL RHAKWFQARA NGLKSCVIVI RVLRDLCTRV PTWGPLRGWP 250
    LELLCEKSIG TANRPMGAGE ALRRVLECLA SGIVMPDGSG IYDPCEKEAT 300
    DAIGHLDRQQ REDITQSAQH ALRLAAFGQL HKVLGMDPLP SKMPKKPKNE 350
    NPVDYTVQIP PSTTYAITPM KRPMEEDGEE KSPSKKKKKI QKKEEKAEPP 400
    QAMNALMRLN QLKPGLQYKL ISQTGPVHAP IFTMSVEVDG STFEASGPSK 450
    KTAKLHVAVK VLQDMGLPTG AEGRDSSKGE DSAEESDGKP AVVAPPPVVE 500
    AVSNPSSVFP SDATTEQGPI LTKHGKNPVM ELNEKRRGLK YELISETGGS 550
    HDKRFVMEVE VDGQKFQGAG SNKKVAKAYA ALAALEKLFP DAPLALEANK 600
    KKRAPVPVRG GPKFAAKPHN PGFGMGGPMH NEAPPPPNIR GRGRGGNIRG 650
    RGRGRGFGGT NHGGGYMNAG AGYGSYGYSS NSATAGYSQF YSNGGHYGNA 700
    GGGGSGGGGG SSSYSSYYQG DSYNSPVPPK HAGKKPLHGG QQKPSYSSGY 750
    QSHQGQQQPY NQSQYSSYGT PQGKQKGYGH GQGSYSSYSN SYNSPGGGGG 800
    SDYSYDSKFN YSGSGGRSGG NSYGSSGSSY NTGSHGGYGA GSGGSSSYQG 850
    KQGGYSSQSN YSSPGSSQSY SGPASSYQSS QGGYSRNTEH SMNYQYR 897
    Length:897
    Mass (Da):95,935
    Last modified:April 12, 2005 - v2
    Checksum:iACD800D1858539BB
    GO
    Isoform 2 (identifier: Q9JIL3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MALYHHHFITRRRR

    Show »
    Length:910
    Mass (Da):97,680
    Checksum:iD6AB97A4B52E442A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MALYHHHFITRRRR in isoform 2. 1 PublicationVSP_013409

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF220102 mRNA. Translation: AAF31446.1.
    AABR03063190 Genomic DNA. No translation available.
    AABR03065470 Genomic DNA. No translation available.
    RefSeqiNP_445864.1. NM_053412.1. [Q9JIL3-2]
    UniGeneiRn.203881.

    Genome annotation databases

    GeneIDi84472.
    KEGGirno:84472.
    UCSCiRGD:619734. rat. [Q9JIL3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF220102 mRNA. Translation: AAF31446.1 .
    AABR03063190 Genomic DNA. No translation available.
    AABR03065470 Genomic DNA. No translation available.
    RefSeqi NP_445864.1. NM_053412.1. [Q9JIL3-2 ]
    UniGenei Rn.203881.

    3D structure databases

    ProteinModelPortali Q9JIL3.
    SMRi Q9JIL3. Positions 403-470, 525-589.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249972. 1 interaction.
    IntActi Q9JIL3. 1 interaction.
    STRINGi 10116.ENSRNOP00000062871.

    PTM databases

    PhosphoSitei Q9JIL3.

    Proteomic databases

    PaxDbi Q9JIL3.
    PRIDEi Q9JIL3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 84472.
    KEGGi rno:84472.
    UCSCi RGD:619734. rat. [Q9JIL3-1 ]

    Organism-specific databases

    CTDi 3609.
    RGDi 619734. Ilf3.

    Phylogenomic databases

    eggNOGi NOG307678.
    HOGENOMi HOG000232164.
    HOVERGENi HBG069915.
    InParanoidi Q9JIL3.
    KOi K13090.
    PhylomeDBi Q9JIL3.

    Miscellaneous databases

    NextBioi 616960.
    PROi Q9JIL3.

    Gene expression databases

    Genevestigatori Q9JIL3.

    Family and domain databases

    Gene3Di 3.30.160.20. 2 hits.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR006561. DZF.
    [Graphical view ]
    Pfami PF00035. dsrm. 2 hits.
    PF07528. DZF. 1 hit.
    [Graphical view ]
    SMARTi SM00358. DSRM. 2 hits.
    SM00572. DZF. 1 hit.
    [Graphical view ]
    PROSITEi PS50137. DS_RBD. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Protein-arginine methyltransferase I, the predominant protein-arginine methyltransferase in cells, interacts with and is regulated by interleukin enhancer-binding factor 3."
      Tang J., Kao P.N., Herschman H.R.
      J. Biol. Chem. 275:19866-19876(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    2. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
      Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
      , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
      Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 1).
      Strain: Brown Norway.

    Entry informationi

    Entry nameiILF3_RAT
    AccessioniPrimary (citable) accession number: Q9JIL3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: April 12, 2005
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3