Q9JIL3 (ILF3_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 90.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Interleukin enhancer-binding factor 3 | ||
| Gene names |
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| Organism | Rattus norvegicus (Rat) [Reference proteome] | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus![]() |
Protein attributes
| Sequence length | 897 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA By similarity. Can regulate protein arginine N-methyltransferase 1 activity. |
| Subunit structure | Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with FUS and SMN proteins and with PRMT1. Forms a complex with ILF2. Can also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 and the heterodimeric complex of XRCC6/KU70 and XRCC5/KU80. Forms a heteromeric complex with ZNF346 and ILF3. Found in a nuclear export complex with XPO5, ILF3, Ran and double-stranded RNA or double-stranded minihelix VA1 RNA. Found in a nuclear export complex with XPO5, RAN, ILF3, ZNF346 and double-stranded RNA. Interacts with XPO5 and ZNF346 By similarity. Forms a complex with ILF2, YLPM1, KHDRBS1, RBMX, NCOA5 and PPP1CA. Interacts with EIF2C1 and EIF2C2 By similarity. |
| Subcellular location | Nucleus › nucleolus By similarity. Cytoplasm By similarity. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs By similarity. |
| Post-translational modification | Phosphorylated by RNA-dependent protein kinase (EIF2AK2) By similarity. Methylated by protein arginine N-methyltransferase 1. |
| Sequence similarities | Contains 2 DRBM (double-stranded RNA-binding) domains. Contains 1 DZF domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Prmt1 | Q63009 | 2 | EBI-78714,EBI-78708 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9JIL3-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9JIL3-2) The sequence of this isoform differs from the canonical sequence as follows: 1-1: M → MALYHHHFITRRRR |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 897 | 897 | Interleukin enhancer-binding factor 3 | PRO_0000126072 | |||||
Regions | |||||||||
| Domain | 94 – 342 | 249 | DZF | ||||||
| Domain | 398 – 467 | 70 | DRBM 1 | ||||||
| Domain | 524 – 590 | 67 | DRBM 2 | ||||||
| Region | 609 – 897 | 289 | Interaction with PRMT1 | ||||||
| Motif | 371 – 389 | 19 | Bipartite nuclear localization signal Potential | ||||||
| Compositional bias | 385 – 389 | 5 | Poly-Lys | ||||||
| Compositional bias | 634 – 637 | 4 | Poly-Pro | ||||||
| Compositional bias | 640 – 659 | 20 | Arg/Gly-rich | ||||||
| Compositional bias | 701 – 710 | 10 | Poly-Gly | ||||||
| Compositional bias | 796 – 800 | 5 | Poly-Gly | ||||||
Amino acid modifications | |||||||||
| Modified residue | 100 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 190 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 382 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 384 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 460 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 476 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 482 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 609 | 1 | Omega-N-methylated arginine By similarity | ||||||
| Modified residue | 794 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 814 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 | 1 | M → MALYHHHFITRRRR in isoform 2. | VSP_013409 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Protein-arginine methyltransferase I, the predominant protein-arginine methyltransferase in cells, interacts with and is regulated by interleukin enhancer-binding factor 3." Tang J., Kao P.N., Herschman H.R. J. Biol. Chem. 275:19866-19876(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). |
| [2] | "Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. Collins F.S.Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 1). Strain: Brown Norway. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF220102 mRNA. Translation: AAF31446.1. AABR03063190 Genomic DNA. No translation available. AABR03065470 Genomic DNA. No translation available. |
| IPI | IPI00200674. IPI00555218. |
| RefSeq | NP_445864.1. NM_053412.1. |
| UniGene | Rn.203881. |
3D structure databases | |
| ProteinModelPortal | Q9JIL3. |
| SMR | Q9JIL3. Positions 403-470, 525-589. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9JIL3. 1 interaction. |
| STRING | 10116.ENSRNOP00000062871. |
PTM databases | |
| PhosphoSite | Q9JIL3. |
Proteomic databases | |
| PaxDb | Q9JIL3. |
| PRIDE | Q9JIL3. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 84472. |
| KEGG | rno:84472. |
| UCSC | RGD:619734. rat. |
Organism-specific databases | |
| CTD | 3609. |
| RGD | 619734. Ilf3. |
Phylogenomic databases | |
| eggNOG | NOG307678. |
| HOGENOM | HOG000232164. |
| HOVERGEN | HBG069915. |
| InParanoid | Q9JIL3. |
| KO | K13090. |
| OrthoDB | EOG46WZ80. |
Gene expression databases | |
| ArrayExpress | Q9JIL3. |
| Genevestigator | Q9JIL3. |
| GermOnline | ENSRNOG00000022741. Rattus norvegicus. |
Family and domain databases | |
| Gene3D | 3.30.160.20. 2 hits. |
| InterPro | IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR006561. DZF. [Graphical view] |
| Pfam | PF00035. dsrm. 2 hits. PF07528. DZF. 1 hit. [Graphical view] |
| SMART | SM00358. DSRM. 2 hits. SM00572. DZF. 1 hit. [Graphical view] |
| PROSITE | PS50137. DS_RBD. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 616960. |
Entry information
| Entry name | ILF3_RAT | ||||||||
| Accession | Primary (citable) accession number: Q9JIL3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with
