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Protein

Alcohol dehydrogenase [NADP(+)]

Gene

Akr1a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs (By similarity).By similarity

Catalytic activityi

An alcohol + NADP+ = an aldehyde + NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei50Proton donorBy similarity1
Sitei80Lowers pKa of active site TyrBy similarity1
Binding sitei113SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 20NADPSequence analysis10
Nucleotide bindingi211 – 273NADPBy similarityAdd BLAST63

GO - Molecular functioni

  • alcohol dehydrogenase (NADP+) activity Source: UniProtKB
  • alditol:NADP+ 1-oxidoreductase activity Source: MGI
  • L-glucuronate reductase activity Source: MGI

GO - Biological processi

  • aldehyde catabolic process Source: MGI
  • D-glucuronate catabolic process Source: MGI
  • L-ascorbic acid biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.2. 3474.
ReactomeiR-MMU-156590. Glutathione conjugation.
R-MMU-5661270. Catabolism of glucuronate to xylulose-5-phosphate.
SABIO-RKQ9JII6.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase [NADP(+)] (EC:1.1.1.2)
Alternative name(s):
Aldehyde reductase
Aldo-keto reductase family 1 member A1
Gene namesi
Name:Akr1a1
Synonyms:Akr1a4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1929955. Akr1a1.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001246182 – 325Alcohol dehydrogenase [NADP(+)]Add BLAST324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonine1 Publication1
Modified residuei4PhosphoserineBy similarity1
Modified residuei38PhosphoserineBy similarity1
Modified residuei127N6-acetyllysine; alternateCombined sources1
Modified residuei127N6-succinyllysine; alternateCombined sources1
Modified residuei145N6-succinyllysineCombined sources1
Modified residuei211PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JII6.
MaxQBiQ9JII6.
PaxDbiQ9JII6.
PRIDEiQ9JII6.

2D gel databases

REPRODUCTION-2DPAGEIPI00466128.
Q9JII6.

PTM databases

iPTMnetiQ9JII6.
PhosphoSitePlusiQ9JII6.
SwissPalmiQ9JII6.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Detected at high levels in several tissues including neural ectoderm, gut endoderm, somites, branchial arches, otic vesicles, limb buds and tail bud.

Inductioni

Fear memory increases expression 7-fold.1 Publication

Gene expression databases

BgeeiENSMUSG00000028692.
ExpressionAtlasiQ9JII6. baseline and differential.
GenevisibleiQ9JII6. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ9JII6. 5 interactors.
MINTiMINT-1869516.
STRINGi10090.ENSMUSP00000030455.

Structurei

Secondary structure

1325
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Beta strandi13 – 17Combined sources5
Helixi26 – 38Combined sources13
Beta strandi43 – 45Combined sources3
Helixi48 – 50Combined sources3
Helixi53 – 63Combined sources11
Beta strandi68 – 70Combined sources3
Helixi72 – 74Combined sources3
Beta strandi76 – 81Combined sources6
Helixi83 – 85Combined sources3
Helixi88 – 102Combined sources15
Beta strandi107 – 113Combined sources7
Beta strandi115 – 118Combined sources4
Beta strandi120 – 122Combined sources3
Helixi140 – 152Combined sources13
Beta strandi155 – 157Combined sources3
Beta strandi159 – 163Combined sources5
Helixi166 – 175Combined sources10
Beta strandi182 – 186Combined sources5
Helixi194 – 202Combined sources9
Beta strandi206 – 211Combined sources6
Helixi216 – 221Combined sources6
Helixi228 – 230Combined sources3
Helixi232 – 241Combined sources10
Helixi245 – 255Combined sources11
Helixi267 – 273Combined sources7
Helixi283 – 290Combined sources8
Beta strandi302 – 305Combined sources4
Beta strandi308 – 313Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GACX-ray1.64A/B2-325[»]
ProteinModelPortaliQ9JII6.
SMRiQ9JII6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOVERGENiHBG000020.
InParanoidiQ9JII6.
KOiK00002.
OMAiWDALEKI.
OrthoDBiEOG091G0D69.
PhylomeDBiQ9JII6.
TreeFamiTF106492.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JII6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTASSVLLHT GQKMPLIGLG TWKSEPGQVK AAIKHALSAG YRHIDCASVY
60 70 80 90 100
GNETEIGEAL KESVGSGKAV PREELFVTSK LWNTKHHPED VEPALRKTLA
110 120 130 140 150
DLQLEYLDLY LMHWPYAFER GDNPFPKNAD GTVRYDSTHY KETWKALEVL
160 170 180 190 200
VAKGLVKALG LSNFNSRQID DVLSVASVRP AVLQVECHPY LAQNELIAHC
210 220 230 240 250
HARGLEVTAY SPLGSSDRAW RHPDEPVLLE EPVVLALAEK HGRSPAQILL
260 270 280 290 300
RWQVQRKVIC IPKSINPSRI LQNIQVFDFT FSPEEMKQLD ALNKNWRYIV
310 320
PMITVDGKRV PRDAGHPLYP FNDPY
Length:325
Mass (Da):36,587
Last modified:January 23, 2007 - v3
Checksum:i0097939061DA34CB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54T → A in BAB27586 (PubMed:16141072).Curated1
Sequence conflicti60L → P in BAB27915 (PubMed:16141072).Curated1
Sequence conflicti95L → I in BAB27883 (PubMed:16141072).Curated1
Sequence conflicti95L → I in BAB27767 (PubMed:16141072).Curated1
Sequence conflicti120R → L in BAB27909 (PubMed:16141072).Curated1
Sequence conflicti284E → G in BAB27437 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225564 mRNA. Translation: AAF67111.1.
AK011906 mRNA. Translation: BAB27907.1.
AK009462 mRNA. Translation: BAB26303.1.
AK011321 mRNA. Translation: BAB27543.1.
AK011794 mRNA. Translation: BAB27846.1.
AK011908 mRNA. Translation: BAB27909.1.
AK011918 mRNA. Translation: BAB27915.1.
AK011856 mRNA. Translation: BAB27883.1.
AK011667 mRNA. Translation: BAB27767.1.
AK011388 mRNA. Translation: BAB27586.1.
AK011157 mRNA. Translation: BAB27437.1.
AK011209 mRNA. Translation: BAB27469.1.
AK005162 mRNA. Translation: BAB23853.1.
AK011221 mRNA. No translation available.
BC039926 mRNA. Translation: AAH39926.1.
CCDSiCCDS18514.1.
RefSeqiNP_067448.1. NM_021473.3.
UniGeneiMm.30085.
Mm.467921.
Mm.489607.

Genome annotation databases

EnsembliENSMUST00000030455; ENSMUSP00000030455; ENSMUSG00000028692.
GeneIDi58810.
KEGGimmu:58810.
UCSCiuc008uha.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225564 mRNA. Translation: AAF67111.1.
AK011906 mRNA. Translation: BAB27907.1.
AK009462 mRNA. Translation: BAB26303.1.
AK011321 mRNA. Translation: BAB27543.1.
AK011794 mRNA. Translation: BAB27846.1.
AK011908 mRNA. Translation: BAB27909.1.
AK011918 mRNA. Translation: BAB27915.1.
AK011856 mRNA. Translation: BAB27883.1.
AK011667 mRNA. Translation: BAB27767.1.
AK011388 mRNA. Translation: BAB27586.1.
AK011157 mRNA. Translation: BAB27437.1.
AK011209 mRNA. Translation: BAB27469.1.
AK005162 mRNA. Translation: BAB23853.1.
AK011221 mRNA. No translation available.
BC039926 mRNA. Translation: AAH39926.1.
CCDSiCCDS18514.1.
RefSeqiNP_067448.1. NM_021473.3.
UniGeneiMm.30085.
Mm.467921.
Mm.489607.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GACX-ray1.64A/B2-325[»]
ProteinModelPortaliQ9JII6.
SMRiQ9JII6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JII6. 5 interactors.
MINTiMINT-1869516.
STRINGi10090.ENSMUSP00000030455.

PTM databases

iPTMnetiQ9JII6.
PhosphoSitePlusiQ9JII6.
SwissPalmiQ9JII6.

2D gel databases

REPRODUCTION-2DPAGEIPI00466128.
Q9JII6.

Proteomic databases

EPDiQ9JII6.
MaxQBiQ9JII6.
PaxDbiQ9JII6.
PRIDEiQ9JII6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030455; ENSMUSP00000030455; ENSMUSG00000028692.
GeneIDi58810.
KEGGimmu:58810.
UCSCiuc008uha.2. mouse.

Organism-specific databases

CTDi10327.
MGIiMGI:1929955. Akr1a1.

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOVERGENiHBG000020.
InParanoidiQ9JII6.
KOiK00002.
OMAiWDALEKI.
OrthoDBiEOG091G0D69.
PhylomeDBiQ9JII6.
TreeFamiTF106492.

Enzyme and pathway databases

BRENDAi1.1.1.2. 3474.
ReactomeiR-MMU-156590. Glutathione conjugation.
R-MMU-5661270. Catabolism of glucuronate to xylulose-5-phosphate.
SABIO-RKQ9JII6.

Miscellaneous databases

PROiQ9JII6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028692.
ExpressionAtlasiQ9JII6. baseline and differential.
GenevisibleiQ9JII6. MM.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAK1A1_MOUSE
AccessioniPrimary (citable) accession number: Q9JII6
Secondary accession number(s): Q9CQI5
, Q9CQT8, Q9CT53, Q9D012, Q9D016, Q9D0I7, Q9D0P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.