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Q9JII5

- DAZP1_MOUSE

UniProt

Q9JII5 - DAZP1_MOUSE

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Protein

DAZ-associated protein 1

Gene

Dazap1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

RNA-binding protein, which may be required during spermatogenesis.By similarity

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. poly(A) RNA binding Source: Ensembl
  3. RNA binding Source: MGI
  4. RNA stem-loop binding Source: MGI

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. cell proliferation Source: MGI
  3. maternal placenta development Source: MGI
  4. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DAZ-associated protein 1
Alternative name(s):
Deleted in azoospermia-associated protein 1
Gene namesi
Name:Dazap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1917498. Dazap1.

Subcellular locationi

Cytoplasm. Nucleus
Note: Predominantly cytoplasmic. Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406DAZ-associated protein 1PRO_0000081566Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei150 – 1501N6-acetyllysineBy similarity

Post-translational modificationi

Acetylation at Lys-150 is predominantly observed in the nuclear fraction, and may regulate nucleocytoplasmic transport.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9JII5.
PaxDbiQ9JII5.
PRIDEiQ9JII5.

2D gel databases

REPRODUCTION-2DPAGEQ9JII5.

PTM databases

PhosphoSiteiQ9JII5.

Expressioni

Tissue specificityi

Mainly expressed in testis. Expressed at much lower level in liver, heart and brain. Also expressed in ovary. Expressed throughout testes development, in both the prenatal and postnatal periods.1 Publication

Developmental stagei

First expressed in midpachytene spermatocytes in stage VII tubules.

Gene expression databases

BgeeiQ9JII5.
CleanExiMM_DAZAP1.
ExpressionAtlasiQ9JII5. baseline and differential.
GenevestigatoriQ9JII5.

Interactioni

Subunit structurei

Interacts with DAZ and DAZL.By similarity

Protein-protein interaction databases

IntActiQ9JII5. 1 interaction.
MINTiMINT-4092839.

Structurei

3D structure databases

ProteinModelPortaliQ9JII5.
SMRiQ9JII5. Positions 6-198.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 9788RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini113 – 19078RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi222 – 384163Pro-richAdd
BLAST

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00760000118873.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiQ9JII5.
KOiK14411.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9JII5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSAGADEIG KLFVGGLDWS TTQETLRSYF SQYGEVVDCV IMKDKTTNQS
60 70 80 90 100
RGFGFVKFKD PNCVGTVLAS RPHTLDGRNI DPKPCTPRGM QPERTRPKEG
110 120 130 140 150
WQKGPRSDSS KSNKIFVGGI PHNCGETELR EYFKKFGVVT EVVMIYDAEK
160 170 180 190 200
QRPRGFGFIT FEDEQSVDQA VNMHFHDIMG KKVEVKRAEP RDSKNQAPGQ
210 220 230 240 250
PGASQWGSRV APSAANGWAG QPPPTWQQGY GPQGMWVPAG QAIGGYGPPP
260 270 280 290 300
AGRGAPPPPP PFTSYIVSTP PGGFPPPQGF PQGYGAPPQF SFGYGPPPPP
310 320 330 340 350
PDQFAPPGVP PPPATPGAAP LAFPPPPSQA APDMSKPPTA QPDFPYGQYG
360 370 380 390 400
YGQDLSGFGQ GFSDPSQQPP SYGGPSVPGS GGPPAGGSGF GRGQNHNVQG

FHPYRR
Length:406
Mass (Da):43,214
Last modified:March 1, 2004 - v2
Checksum:iB0C71A7AED2F50D7
GO
Isoform 2 (identifier: Q9JII5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-102: Missing.

Note: No experimental confirmation available.

Show »
Length:405
Mass (Da):43,086
Checksum:i4CE8B2223B0A9391
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti241 – 2411Q → R in AAF81071. (PubMed:11604102)Curated
Sequence conflicti358 – 3581F → L in AAF81071. (PubMed:11604102)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei102 – 1021Missing in isoform 2. 1 PublicationVSP_009443

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225910 mRNA. Translation: AAF81071.1.
BC049355 mRNA. Translation: AAH49355.1.
CCDSiCCDS35977.1. [Q9JII5-2]
CCDS48626.1. [Q9JII5-1]
RefSeqiNP_001116076.1. NM_001122604.1. [Q9JII5-1]
NP_001116077.1. NM_001122605.1.
NP_573451.2. NM_133188.2. [Q9JII5-2]
UniGeneiMm.148693.

Genome annotation databases

EnsembliENSMUST00000092305; ENSMUSP00000089958; ENSMUSG00000069565. [Q9JII5-1]
ENSMUST00000105362; ENSMUSP00000101001; ENSMUSG00000069565. [Q9JII5-2]
GeneIDi70248.
KEGGimmu:70248.
UCSCiuc007gcl.2. mouse. [Q9JII5-2]
uc007gcm.2. mouse. [Q9JII5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225910 mRNA. Translation: AAF81071.1 .
BC049355 mRNA. Translation: AAH49355.1 .
CCDSi CCDS35977.1. [Q9JII5-2 ]
CCDS48626.1. [Q9JII5-1 ]
RefSeqi NP_001116076.1. NM_001122604.1. [Q9JII5-1 ]
NP_001116077.1. NM_001122605.1.
NP_573451.2. NM_133188.2. [Q9JII5-2 ]
UniGenei Mm.148693.

3D structure databases

ProteinModelPortali Q9JII5.
SMRi Q9JII5. Positions 6-198.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9JII5. 1 interaction.
MINTi MINT-4092839.

PTM databases

PhosphoSitei Q9JII5.

2D gel databases

REPRODUCTION-2DPAGE Q9JII5.

Proteomic databases

MaxQBi Q9JII5.
PaxDbi Q9JII5.
PRIDEi Q9JII5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000092305 ; ENSMUSP00000089958 ; ENSMUSG00000069565 . [Q9JII5-1 ]
ENSMUST00000105362 ; ENSMUSP00000101001 ; ENSMUSG00000069565 . [Q9JII5-2 ]
GeneIDi 70248.
KEGGi mmu:70248.
UCSCi uc007gcl.2. mouse. [Q9JII5-2 ]
uc007gcm.2. mouse. [Q9JII5-1 ]

Organism-specific databases

CTDi 26528.
MGIi MGI:1917498. Dazap1.

Phylogenomic databases

eggNOGi COG0724.
GeneTreei ENSGT00760000118873.
HOGENOMi HOG000234441.
HOVERGENi HBG002295.
InParanoidi Q9JII5.
KOi K14411.

Miscellaneous databases

ChiTaRSi Dazap1. mouse.
NextBioi 331250.
PROi Q9JII5.
SOURCEi Search...

Gene expression databases

Bgeei Q9JII5.
CleanExi MM_DAZAP1.
ExpressionAtlasi Q9JII5. baseline and differential.
Genevestigatori Q9JII5.

Family and domain databases

Gene3Di 3.30.70.330. 2 hits.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF00076. RRM_1. 2 hits.
[Graphical view ]
SMARTi SM00360. RRM. 2 hits.
[Graphical view ]
PROSITEi PS50102. RRM. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the mouse Dazap1 gene encoding an RNA-binding protein that interacts with infertility factors DAZ and DAZL."
    Dai T., Vera Y., Salido E.C., Yen P.H.
    BMC Genomics 2:6-6(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Olfactory epithelium.
  3. "Deleted in azoospermia associated protein 1 shuttles between nucleus and cytoplasm during normal germ cell maturation."
    Vera Y., Dai T., Hikim A.P., Lue Y., Salido E.C., Swerdloff R.S., Yen P.H.
    J. Androl. 23:622-628(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDAZP1_MOUSE
AccessioniPrimary (citable) accession number: Q9JII5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: November 26, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3