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Q9JII5

- DAZP1_MOUSE

UniProt

Q9JII5 - DAZP1_MOUSE

Protein

DAZ-associated protein 1

Gene

Dazap1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (01 Mar 2004)
      Previous versions | rss
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    Functioni

    RNA-binding protein, which may be required during spermatogenesis.By similarity

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. RNA binding Source: MGI
    3. RNA stem-loop binding Source: MGI

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. cell proliferation Source: MGI
    3. maternal placenta development Source: MGI
    4. spermatogenesis Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DAZ-associated protein 1
    Alternative name(s):
    Deleted in azoospermia-associated protein 1
    Gene namesi
    Name:Dazap1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1917498. Dazap1.

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Predominantly cytoplasmic. Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 406406DAZ-associated protein 1PRO_0000081566Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei150 – 1501N6-acetyllysineBy similarity

    Post-translational modificationi

    Acetylation at Lys-150 is predominantly observed in the nuclear fraction, and may regulate nucleocytoplasmic transport.By similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9JII5.
    PaxDbiQ9JII5.
    PRIDEiQ9JII5.

    2D gel databases

    REPRODUCTION-2DPAGEQ9JII5.

    PTM databases

    PhosphoSiteiQ9JII5.

    Expressioni

    Tissue specificityi

    Mainly expressed in testis. Expressed at much lower level in liver, heart and brain. Also expressed in ovary. Expressed throughout testes development, in both the prenatal and postnatal periods.1 Publication

    Developmental stagei

    First expressed in midpachytene spermatocytes in stage VII tubules.

    Gene expression databases

    ArrayExpressiQ9JII5.
    BgeeiQ9JII5.
    CleanExiMM_DAZAP1.
    GenevestigatoriQ9JII5.

    Interactioni

    Subunit structurei

    Interacts with DAZ and DAZL.By similarity

    Protein-protein interaction databases

    IntActiQ9JII5. 1 interaction.
    MINTiMINT-4092839.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JII5.
    SMRiQ9JII5. Positions 6-198.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 9788RRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini113 – 19078RRM 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi222 – 384163Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0724.
    GeneTreeiENSGT00730000110584.
    HOGENOMiHOG000234441.
    HOVERGENiHBG002295.
    InParanoidiQ9JII5.
    KOiK14411.

    Family and domain databases

    Gene3Di3.30.70.330. 2 hits.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF00076. RRM_1. 2 hits.
    [Graphical view]
    SMARTiSM00360. RRM. 2 hits.
    [Graphical view]
    PROSITEiPS50102. RRM. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JII5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNSAGADEIG KLFVGGLDWS TTQETLRSYF SQYGEVVDCV IMKDKTTNQS    50
    RGFGFVKFKD PNCVGTVLAS RPHTLDGRNI DPKPCTPRGM QPERTRPKEG 100
    WQKGPRSDSS KSNKIFVGGI PHNCGETELR EYFKKFGVVT EVVMIYDAEK 150
    QRPRGFGFIT FEDEQSVDQA VNMHFHDIMG KKVEVKRAEP RDSKNQAPGQ 200
    PGASQWGSRV APSAANGWAG QPPPTWQQGY GPQGMWVPAG QAIGGYGPPP 250
    AGRGAPPPPP PFTSYIVSTP PGGFPPPQGF PQGYGAPPQF SFGYGPPPPP 300
    PDQFAPPGVP PPPATPGAAP LAFPPPPSQA APDMSKPPTA QPDFPYGQYG 350
    YGQDLSGFGQ GFSDPSQQPP SYGGPSVPGS GGPPAGGSGF GRGQNHNVQG 400
    FHPYRR 406
    Length:406
    Mass (Da):43,214
    Last modified:March 1, 2004 - v2
    Checksum:iB0C71A7AED2F50D7
    GO
    Isoform 2 (identifier: Q9JII5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         102-102: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:405
    Mass (Da):43,086
    Checksum:i4CE8B2223B0A9391
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti241 – 2411Q → R in AAF81071. (PubMed:11604102)Curated
    Sequence conflicti358 – 3581F → L in AAF81071. (PubMed:11604102)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei102 – 1021Missing in isoform 2. 1 PublicationVSP_009443

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF225910 mRNA. Translation: AAF81071.1.
    BC049355 mRNA. Translation: AAH49355.1.
    CCDSiCCDS35977.1. [Q9JII5-2]
    CCDS48626.1. [Q9JII5-1]
    RefSeqiNP_001116076.1. NM_001122604.1. [Q9JII5-1]
    NP_001116077.1. NM_001122605.1.
    NP_573451.2. NM_133188.2. [Q9JII5-2]
    UniGeneiMm.148693.

    Genome annotation databases

    EnsembliENSMUST00000092305; ENSMUSP00000089958; ENSMUSG00000069565. [Q9JII5-1]
    ENSMUST00000105362; ENSMUSP00000101001; ENSMUSG00000069565. [Q9JII5-2]
    GeneIDi70248.
    KEGGimmu:70248.
    UCSCiuc007gcl.2. mouse. [Q9JII5-2]
    uc007gcm.2. mouse. [Q9JII5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF225910 mRNA. Translation: AAF81071.1 .
    BC049355 mRNA. Translation: AAH49355.1 .
    CCDSi CCDS35977.1. [Q9JII5-2 ]
    CCDS48626.1. [Q9JII5-1 ]
    RefSeqi NP_001116076.1. NM_001122604.1. [Q9JII5-1 ]
    NP_001116077.1. NM_001122605.1.
    NP_573451.2. NM_133188.2. [Q9JII5-2 ]
    UniGenei Mm.148693.

    3D structure databases

    ProteinModelPortali Q9JII5.
    SMRi Q9JII5. Positions 6-198.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9JII5. 1 interaction.
    MINTi MINT-4092839.

    PTM databases

    PhosphoSitei Q9JII5.

    2D gel databases

    REPRODUCTION-2DPAGE Q9JII5.

    Proteomic databases

    MaxQBi Q9JII5.
    PaxDbi Q9JII5.
    PRIDEi Q9JII5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000092305 ; ENSMUSP00000089958 ; ENSMUSG00000069565 . [Q9JII5-1 ]
    ENSMUST00000105362 ; ENSMUSP00000101001 ; ENSMUSG00000069565 . [Q9JII5-2 ]
    GeneIDi 70248.
    KEGGi mmu:70248.
    UCSCi uc007gcl.2. mouse. [Q9JII5-2 ]
    uc007gcm.2. mouse. [Q9JII5-1 ]

    Organism-specific databases

    CTDi 26528.
    MGIi MGI:1917498. Dazap1.

    Phylogenomic databases

    eggNOGi COG0724.
    GeneTreei ENSGT00730000110584.
    HOGENOMi HOG000234441.
    HOVERGENi HBG002295.
    InParanoidi Q9JII5.
    KOi K14411.

    Miscellaneous databases

    ChiTaRSi DAZAP1. mouse.
    NextBioi 331250.
    PROi Q9JII5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JII5.
    Bgeei Q9JII5.
    CleanExi MM_DAZAP1.
    Genevestigatori Q9JII5.

    Family and domain databases

    Gene3Di 3.30.70.330. 2 hits.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF00076. RRM_1. 2 hits.
    [Graphical view ]
    SMARTi SM00360. RRM. 2 hits.
    [Graphical view ]
    PROSITEi PS50102. RRM. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the mouse Dazap1 gene encoding an RNA-binding protein that interacts with infertility factors DAZ and DAZL."
      Dai T., Vera Y., Salido E.C., Yen P.H.
      BMC Genomics 2:6-6(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Olfactory epithelium.
    3. "Deleted in azoospermia associated protein 1 shuttles between nucleus and cytoplasm during normal germ cell maturation."
      Vera Y., Dai T., Hikim A.P., Lue Y., Salido E.C., Swerdloff R.S., Yen P.H.
      J. Androl. 23:622-628(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiDAZP1_MOUSE
    AccessioniPrimary (citable) accession number: Q9JII5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3