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Protein

DAZ-associated protein 1

Gene

Dazap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

RNA-binding protein, which may be required during spermatogenesis.By similarity

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: Ensembl
  • RNA binding Source: MGI
  • RNA stem-loop binding Source: MGI

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • cell proliferation Source: MGI
  • maternal placenta development Source: MGI
  • positive regulation of mRNA splicing, via spliceosome Source: MGI
  • spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DAZ-associated protein 1
Alternative name(s):
Deleted in azoospermia-associated protein 1
Gene namesi
Name:Dazap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1917498. Dazap1.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Predominantly cytoplasmic. Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: Ensembl
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406DAZ-associated protein 1PRO_0000081566Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei150 – 1501N6-acetyllysineBy similarity

Post-translational modificationi

Acetylation at Lys-150 is predominantly observed in the nuclear fraction, and may regulate nucleocytoplasmic transport.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9JII5.
PaxDbiQ9JII5.
PRIDEiQ9JII5.

2D gel databases

REPRODUCTION-2DPAGEQ9JII5.

PTM databases

PhosphoSiteiQ9JII5.

Expressioni

Tissue specificityi

Mainly expressed in testis. Expressed at much lower level in liver, heart and brain. Also expressed in ovary. Expressed throughout testes development, in both the prenatal and postnatal periods.1 Publication

Developmental stagei

First expressed in midpachytene spermatocytes in stage VII tubules.

Gene expression databases

BgeeiQ9JII5.
CleanExiMM_DAZAP1.
ExpressionAtlasiQ9JII5. baseline and differential.
GenevisibleiQ9JII5. MM.

Interactioni

Subunit structurei

Interacts with DAZ and DAZL.By similarity

Protein-protein interaction databases

IntActiQ9JII5. 1 interaction.
MINTiMINT-4092839.
STRINGi10090.ENSMUSP00000089958.

Structurei

3D structure databases

ProteinModelPortaliQ9JII5.
SMRiQ9JII5. Positions 6-198.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 9788RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini113 – 19078RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi222 – 384163Pro-richAdd
BLAST

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00730000110584.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiQ9JII5.
KOiK14411.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JII5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSAGADEIG KLFVGGLDWS TTQETLRSYF SQYGEVVDCV IMKDKTTNQS
60 70 80 90 100
RGFGFVKFKD PNCVGTVLAS RPHTLDGRNI DPKPCTPRGM QPERTRPKEG
110 120 130 140 150
WQKGPRSDSS KSNKIFVGGI PHNCGETELR EYFKKFGVVT EVVMIYDAEK
160 170 180 190 200
QRPRGFGFIT FEDEQSVDQA VNMHFHDIMG KKVEVKRAEP RDSKNQAPGQ
210 220 230 240 250
PGASQWGSRV APSAANGWAG QPPPTWQQGY GPQGMWVPAG QAIGGYGPPP
260 270 280 290 300
AGRGAPPPPP PFTSYIVSTP PGGFPPPQGF PQGYGAPPQF SFGYGPPPPP
310 320 330 340 350
PDQFAPPGVP PPPATPGAAP LAFPPPPSQA APDMSKPPTA QPDFPYGQYG
360 370 380 390 400
YGQDLSGFGQ GFSDPSQQPP SYGGPSVPGS GGPPAGGSGF GRGQNHNVQG

FHPYRR
Length:406
Mass (Da):43,214
Last modified:March 1, 2004 - v2
Checksum:iB0C71A7AED2F50D7
GO
Isoform 2 (identifier: Q9JII5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-102: Missing.

Note: No experimental confirmation available.
Show »
Length:405
Mass (Da):43,086
Checksum:i4CE8B2223B0A9391
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti241 – 2411Q → R in AAF81071 (PubMed:11604102).Curated
Sequence conflicti358 – 3581F → L in AAF81071 (PubMed:11604102).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei102 – 1021Missing in isoform 2. 1 PublicationVSP_009443

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225910 mRNA. Translation: AAF81071.1.
BC049355 mRNA. Translation: AAH49355.1.
CCDSiCCDS35977.1. [Q9JII5-2]
CCDS48626.1. [Q9JII5-1]
RefSeqiNP_001116076.1. NM_001122604.1. [Q9JII5-1]
NP_001116077.1. NM_001122605.1.
NP_573451.2. NM_133188.2. [Q9JII5-2]
UniGeneiMm.148693.

Genome annotation databases

EnsembliENSMUST00000092305; ENSMUSP00000089958; ENSMUSG00000069565. [Q9JII5-1]
ENSMUST00000105362; ENSMUSP00000101001; ENSMUSG00000069565. [Q9JII5-2]
GeneIDi70248.
KEGGimmu:70248.
UCSCiuc007gcl.2. mouse. [Q9JII5-2]
uc007gcm.2. mouse. [Q9JII5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225910 mRNA. Translation: AAF81071.1.
BC049355 mRNA. Translation: AAH49355.1.
CCDSiCCDS35977.1. [Q9JII5-2]
CCDS48626.1. [Q9JII5-1]
RefSeqiNP_001116076.1. NM_001122604.1. [Q9JII5-1]
NP_001116077.1. NM_001122605.1.
NP_573451.2. NM_133188.2. [Q9JII5-2]
UniGeneiMm.148693.

3D structure databases

ProteinModelPortaliQ9JII5.
SMRiQ9JII5. Positions 6-198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JII5. 1 interaction.
MINTiMINT-4092839.
STRINGi10090.ENSMUSP00000089958.

PTM databases

PhosphoSiteiQ9JII5.

2D gel databases

REPRODUCTION-2DPAGEQ9JII5.

Proteomic databases

MaxQBiQ9JII5.
PaxDbiQ9JII5.
PRIDEiQ9JII5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092305; ENSMUSP00000089958; ENSMUSG00000069565. [Q9JII5-1]
ENSMUST00000105362; ENSMUSP00000101001; ENSMUSG00000069565. [Q9JII5-2]
GeneIDi70248.
KEGGimmu:70248.
UCSCiuc007gcl.2. mouse. [Q9JII5-2]
uc007gcm.2. mouse. [Q9JII5-1]

Organism-specific databases

CTDi26528.
MGIiMGI:1917498. Dazap1.

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00730000110584.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiQ9JII5.
KOiK14411.

Miscellaneous databases

ChiTaRSiDazap1. mouse.
NextBioi331250.
PROiQ9JII5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JII5.
CleanExiMM_DAZAP1.
ExpressionAtlasiQ9JII5. baseline and differential.
GenevisibleiQ9JII5. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the mouse Dazap1 gene encoding an RNA-binding protein that interacts with infertility factors DAZ and DAZL."
    Dai T., Vera Y., Salido E.C., Yen P.H.
    BMC Genomics 2:6-6(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Olfactory epithelium.
  3. "Deleted in azoospermia associated protein 1 shuttles between nucleus and cytoplasm during normal germ cell maturation."
    Vera Y., Dai T., Hikim A.P., Lue Y., Salido E.C., Swerdloff R.S., Yen P.H.
    J. Androl. 23:622-628(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDAZP1_MOUSE
AccessioniPrimary (citable) accession number: Q9JII5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: July 22, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.