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Q9JII5 (DAZP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DAZ-associated protein 1
Alternative name(s):
Deleted in azoospermia-associated protein 1
Gene names
Name:Dazap1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

RNA-binding protein, which may be required during spermatogenesis By similarity.

Subunit structure

Interacts with DAZ and DAZL By similarity.

Subcellular location

Cytoplasm. Nucleus. Note: Predominantly cytoplasmic. Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm. Ref.1 Ref.3

Tissue specificity

Mainly expressed in testis. Expressed at much lower level in liver, heart and brain. Also expressed in ovary. Expressed throughout testes development, in both the prenatal and postnatal periods. Ref.1

Developmental stage

First expressed in midpachytene spermatocytes in stage VII tubules.

Post-translational modification

Acetylation at Lys-150 is predominantly observed in the nuclear fraction, and may regulate nucleocytoplasmic transport By similarity.

Sequence similarities

Contains 2 RRM (RNA recognition motif) domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JII5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JII5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     102-102: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 406406DAZ-associated protein 1
PRO_0000081566

Regions

Domain10 – 9788RRM 1
Domain113 – 19078RRM 2
Compositional bias222 – 384163Pro-rich

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue1501N6-acetyllysine By similarity

Natural variations

Alternative sequence1021Missing in isoform 2.
VSP_009443

Experimental info

Sequence conflict2411Q → R in AAF81071. Ref.1
Sequence conflict3581F → L in AAF81071. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2004. Version 2.
Checksum: B0C71A7AED2F50D7

FASTA40643,214
        10         20         30         40         50         60 
MNSAGADEIG KLFVGGLDWS TTQETLRSYF SQYGEVVDCV IMKDKTTNQS RGFGFVKFKD 

        70         80         90        100        110        120 
PNCVGTVLAS RPHTLDGRNI DPKPCTPRGM QPERTRPKEG WQKGPRSDSS KSNKIFVGGI 

       130        140        150        160        170        180 
PHNCGETELR EYFKKFGVVT EVVMIYDAEK QRPRGFGFIT FEDEQSVDQA VNMHFHDIMG 

       190        200        210        220        230        240 
KKVEVKRAEP RDSKNQAPGQ PGASQWGSRV APSAANGWAG QPPPTWQQGY GPQGMWVPAG 

       250        260        270        280        290        300 
QAIGGYGPPP AGRGAPPPPP PFTSYIVSTP PGGFPPPQGF PQGYGAPPQF SFGYGPPPPP 

       310        320        330        340        350        360 
PDQFAPPGVP PPPATPGAAP LAFPPPPSQA APDMSKPPTA QPDFPYGQYG YGQDLSGFGQ 

       370        380        390        400 
GFSDPSQQPP SYGGPSVPGS GGPPAGGSGF GRGQNHNVQG FHPYRR 

« Hide

Isoform 2 [UniParc].

Checksum: 4CE8B2223B0A9391
Show »

FASTA40543,086

References

« Hide 'large scale' references
[1]"Characterization of the mouse Dazap1 gene encoding an RNA-binding protein that interacts with infertility factors DAZ and DAZL."
Dai T., Vera Y., Salido E.C., Yen P.H.
BMC Genomics 2:6-6(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Olfactory epithelium.
[3]"Deleted in azoospermia associated protein 1 shuttles between nucleus and cytoplasm during normal germ cell maturation."
Vera Y., Dai T., Hikim A.P., Lue Y., Salido E.C., Swerdloff R.S., Yen P.H.
J. Androl. 23:622-628(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF225910 mRNA. Translation: AAF81071.1.
BC049355 mRNA. Translation: AAH49355.1.
CCDSCCDS35977.1. [Q9JII5-2]
CCDS48626.1. [Q9JII5-1]
RefSeqNP_001116076.1. NM_001122604.1. [Q9JII5-1]
NP_001116077.1. NM_001122605.1.
NP_573451.2. NM_133188.2. [Q9JII5-2]
UniGeneMm.148693.

3D structure databases

ProteinModelPortalQ9JII5.
SMRQ9JII5. Positions 6-198.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9JII5. 1 interaction.
MINTMINT-4092839.

PTM databases

PhosphoSiteQ9JII5.

2D gel databases

REPRODUCTION-2DPAGEQ9JII5.

Proteomic databases

MaxQBQ9JII5.
PaxDbQ9JII5.
PRIDEQ9JII5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000092305; ENSMUSP00000089958; ENSMUSG00000069565. [Q9JII5-1]
ENSMUST00000105362; ENSMUSP00000101001; ENSMUSG00000069565. [Q9JII5-2]
GeneID70248.
KEGGmmu:70248.
UCSCuc007gcl.2. mouse. [Q9JII5-2]
uc007gcm.2. mouse. [Q9JII5-1]

Organism-specific databases

CTD26528.
MGIMGI:1917498. Dazap1.

Phylogenomic databases

eggNOGCOG0724.
GeneTreeENSGT00730000110584.
HOGENOMHOG000234441.
HOVERGENHBG002295.
InParanoidQ9JII5.
KOK14411.

Gene expression databases

ArrayExpressQ9JII5.
BgeeQ9JII5.
CleanExMM_DAZAP1.
GenevestigatorQ9JII5.

Family and domain databases

Gene3D3.30.70.330. 2 hits.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTSM00360. RRM. 2 hits.
[Graphical view]
PROSITEPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDAZAP1. mouse.
NextBio331250.
PROQ9JII5.
SOURCESearch...

Entry information

Entry nameDAZP1_MOUSE
AccessionPrimary (citable) accession number: Q9JII5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot