Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear pore complex protein Nup50

Gene

Nup50

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA transport Source: UniProtKB-KW
  • neural tube formation Source: MGI
  • protein import into nucleus Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-MMU-191859. snRNP Assembly.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-MMU-3371453. Regulation of HSF1-mediated heat shock response.
R-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore complex protein Nup50
Alternative name(s):
50 kDa nucleoporin
Nuclear pore-associated protein 60 kDa-like
Nucleoporin Nup50
Gene namesi
Name:Nup50
Synonyms:Npap60
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1351502. Nup50.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Disruption phenotypei

NUP50 targeted disruption results in a complex phenotype characterized by neural tube defects, exencephaly, intrauterine growth retardation, and late embryonic lethality.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048691 – 466Nuclear pore complex protein Nup50Add BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8N6-acetyllysineCombined sources1
Modified residuei52PhosphoserineBy similarity1
Modified residuei82N6-acetyllysineBy similarity1
Modified residuei126N6-acetyllysineCombined sources1
Modified residuei208PhosphoserineBy similarity1
Modified residuei234PhosphoserineCombined sources1
Modified residuei246PhosphothreonineBy similarity1
Modified residuei268PhosphoserineBy similarity1
Modified residuei294PhosphoserineBy similarity1
Modified residuei448N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JIH2.
MaxQBiQ9JIH2.
PaxDbiQ9JIH2.
PeptideAtlasiQ9JIH2.
PRIDEiQ9JIH2.

PTM databases

iPTMnetiQ9JIH2.
PhosphoSitePlusiQ9JIH2.

Expressioni

Tissue specificityi

Widely expressed at low levels. Highest in the developing neural tube and adult testes.

Gene expression databases

BgeeiENSMUSG00000016619.
GenevisibleiQ9JIH2. MM.

Interactioni

Subunit structurei

Does not interact with TPR (By similarity). Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201817. 2 interactors.
DIPiDIP-46130N.
IntActiQ9JIH2. 2 interactors.
MINTiMINT-4104287.
STRINGi10090.ENSMUSP00000131457.

Structurei

Secondary structure

1466
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni12 – 14Combined sources3
Helixi32 – 35Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C1MX-ray2.20B1-46[»]
ProteinModelPortaliQ9JIH2.
SMRiQ9JIH2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JIH2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati76 – 7712
Repeati112 – 11322
Repeati225 – 22632
Repeati271 – 27242
Repeati301 – 30252
Domaini333 – 466RanBD1PROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 3025 X 2 AA repeats of F-GAdd BLAST227
Regioni143 – 205Binding to CDKN1BAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 90Gly-richAdd BLAST36
Compositional biasi266 – 329Ser-richAdd BLAST64

Domaini

Contains FG repeats.

Sequence similaritiesi

Contains 1 RanBD1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2724. Eukaryota.
ENOG410YPHB. LUCA.
GeneTreeiENSGT00440000035348.
HOVERGENiHBG052697.
InParanoidiQ9JIH2.
KOiK14295.
OMAiKTEDTPD.
OrthoDBiEOG091G0HML.
TreeFamiTF106504.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR015007. NUP2/50/61.
IPR011993. PH_dom-like.
IPR000156. Ran_bind_dom.
[Graphical view]
PfamiPF08911. NUP50. 1 hit.
PF00638. Ran_BP1. 1 hit.
[Graphical view]
SMARTiSM00160. RanBD. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50196. RANBD1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRVAEKEL TDRNWDEEDE VEEMGTFSVA SEEVMKNRAV KKAKRRNVGF
60 70 80 90 100
ESDSGGAFKG FKGLVVPSGG GGFSGFGGSG GKPLEGLTNG NSTDNATPFS
110 120 130 140 150
NVKTAAEPKA AFGSFAVNGP TTLVDKKISS PKCNNSNQPP SSGPASSTAC
160 170 180 190 200
PGNAYHKQLA GLNCSVRDWI VKHVNTNPLC DLTPIFKDYE RYLATIEKQL
210 220 230 240 250
ENGGGSSSES QTDRATAGME PPSLFGSTKL QQESPFSFHG NKAEDTSEKV
260 270 280 290 300
EFTAEKKSDA AQGATSASFS FGKKIESSAL GSLSSGSLTG FSFSAGSSSL
310 320 330 340 350
FGKDAAQSKA ASSLFSAKAS ESPAGGGSSE CRDGEEEEND EPPKVVVTEV
360 370 380 390 400
KEEDAFYSKK CKLFYKKDNE FKEKGVGTLH LKPTATQKTQ LLVRADTNLG
410 420 430 440 450
NILLNVLIAP NMPCTRTGKN NVLIVCVPNP PLDEKQPTLP ATMLIRVKTS
460
EDADELHKIL LEKKDA
Length:466
Mass (Da):49,485
Last modified:July 27, 2011 - v3
Checksum:i8B9216CBB613661A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127 – 135KISSPKCNN → VSNPKTNGD in AAF70057 (PubMed:10811608).Curated9
Sequence conflicti158Q → H in BAE40705 (PubMed:16141072).Curated1
Sequence conflicti314L → P in AAF34721 (PubMed:10891500).Curated1
Sequence conflicti321E → D in AAH60234 (PubMed:16141072).Curated1
Sequence conflicti324A → P in AAF34721 (PubMed:10891500).Curated1
Sequence conflicti439 – 441LPA → IKV in BAB31500 (PubMed:16141072).Curated3
Sequence conflicti451E → K in BAB31500 (PubMed:16141072).Curated1
Sequence conflicti462 – 464EKK → QKT in BAB31500 (PubMed:16141072).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229256 mRNA. Translation: AAF34721.1.
AF251799 mRNA. Translation: AAF70057.1.
AK018983 mRNA. Translation: BAB31500.1.
AK087706 mRNA. Translation: BAC39975.1.
AK133371 mRNA. Translation: BAE21620.1.
AK133562 mRNA. Translation: BAE21727.1.
AK141750 mRNA. Translation: BAE24822.1.
AK151947 mRNA. Translation: BAE30821.1.
AK152309 mRNA. Translation: BAE31115.1.
AK161713 mRNA. Translation: BAE36546.1.
AK165581 mRNA. Translation: BAE38272.1.
AK168888 mRNA. Translation: BAE40705.1.
CH466550 Genomic DNA. Translation: EDL04446.1.
BC059239 mRNA. Translation: AAH59239.1.
BC060234 mRNA. Translation: AAH60234.1.
BC065102 mRNA. Translation: AAH65102.1.
CCDSiCCDS27714.1.
RefSeqiNP_057923.2. NM_016714.2.
UniGeneiMm.28379.
Mm.391486.

Genome annotation databases

EnsembliENSMUST00000165443; ENSMUSP00000131457; ENSMUSG00000016619.
GeneIDi18141.
KEGGimmu:18141.
UCSCiuc007xcq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229256 mRNA. Translation: AAF34721.1.
AF251799 mRNA. Translation: AAF70057.1.
AK018983 mRNA. Translation: BAB31500.1.
AK087706 mRNA. Translation: BAC39975.1.
AK133371 mRNA. Translation: BAE21620.1.
AK133562 mRNA. Translation: BAE21727.1.
AK141750 mRNA. Translation: BAE24822.1.
AK151947 mRNA. Translation: BAE30821.1.
AK152309 mRNA. Translation: BAE31115.1.
AK161713 mRNA. Translation: BAE36546.1.
AK165581 mRNA. Translation: BAE38272.1.
AK168888 mRNA. Translation: BAE40705.1.
CH466550 Genomic DNA. Translation: EDL04446.1.
BC059239 mRNA. Translation: AAH59239.1.
BC060234 mRNA. Translation: AAH60234.1.
BC065102 mRNA. Translation: AAH65102.1.
CCDSiCCDS27714.1.
RefSeqiNP_057923.2. NM_016714.2.
UniGeneiMm.28379.
Mm.391486.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C1MX-ray2.20B1-46[»]
ProteinModelPortaliQ9JIH2.
SMRiQ9JIH2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201817. 2 interactors.
DIPiDIP-46130N.
IntActiQ9JIH2. 2 interactors.
MINTiMINT-4104287.
STRINGi10090.ENSMUSP00000131457.

PTM databases

iPTMnetiQ9JIH2.
PhosphoSitePlusiQ9JIH2.

Proteomic databases

EPDiQ9JIH2.
MaxQBiQ9JIH2.
PaxDbiQ9JIH2.
PeptideAtlasiQ9JIH2.
PRIDEiQ9JIH2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000165443; ENSMUSP00000131457; ENSMUSG00000016619.
GeneIDi18141.
KEGGimmu:18141.
UCSCiuc007xcq.1. mouse.

Organism-specific databases

CTDi10762.
MGIiMGI:1351502. Nup50.

Phylogenomic databases

eggNOGiKOG2724. Eukaryota.
ENOG410YPHB. LUCA.
GeneTreeiENSGT00440000035348.
HOVERGENiHBG052697.
InParanoidiQ9JIH2.
KOiK14295.
OMAiKTEDTPD.
OrthoDBiEOG091G0HML.
TreeFamiTF106504.

Enzyme and pathway databases

ReactomeiR-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-MMU-191859. snRNP Assembly.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-MMU-3371453. Regulation of HSF1-mediated heat shock response.
R-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

ChiTaRSiNup50. mouse.
EvolutionaryTraceiQ9JIH2.
PROiQ9JIH2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000016619.
GenevisibleiQ9JIH2. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR015007. NUP2/50/61.
IPR011993. PH_dom-like.
IPR000156. Ran_bind_dom.
[Graphical view]
PfamiPF08911. NUP50. 1 hit.
PF00638. Ran_BP1. 1 hit.
[Graphical view]
SMARTiSM00160. RanBD. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50196. RANBD1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUP50_MOUSE
AccessioniPrimary (citable) accession number: Q9JIH2
Secondary accession number(s): Q3TG43
, Q3TN17, Q6P1F4, Q6PAL4, Q8C2Y6, Q9CU02, Q9JK85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.