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Q9JIH2

- NUP50_MOUSE

UniProt

Q9JIH2 - NUP50_MOUSE

Protein

Nuclear pore complex protein Nup50

Gene

Nup50

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.1 Publication

    GO - Biological processi

    1. intracellular transport Source: InterPro
    2. mRNA transport Source: UniProtKB-KW
    3. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    mRNA transport, Protein transport, Translocation, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196650. Nuclear Pore Complex (NPC) Disassembly.
    REACT_204192. Regulation of Glucokinase by Glucokinase Regulatory Protein.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear pore complex protein Nup50
    Alternative name(s):
    50 kDa nucleoporin
    Nuclear pore-associated protein 60 kDa-like
    Nucleoporin Nup50
    Gene namesi
    Name:Nup50
    Synonyms:Npap60
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1351502. Nup50.

    Subcellular locationi

    Nucleusnuclear pore complex. Nucleus membrane By similarity; Peripheral membrane protein By similarity; Nucleoplasmic side By similarity
    Note: Localizes to the nucleoplasmic fibrils of the nuclear pore complex. Dissociates from the NPC structure early during prophase of mitosis. Associates to the newly formed nuclear membrane during telophase By similarity.By similarity

    GO - Cellular componenti

    1. nuclear membrane Source: UniProtKB
    2. nuclear pore Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Nuclear pore complex, Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Nup50 are the cause of neural tube defects, exencephaly and intrauterine growth retardation.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 466466Nuclear pore complex protein Nup50PRO_0000204869Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei8 – 81N6-acetyllysine1 Publication
    Modified residuei82 – 821N6-acetyllysineBy similarity
    Modified residuei126 – 1261N6-acetyllysine1 Publication
    Modified residuei246 – 2461PhosphothreonineBy similarity
    Modified residuei294 – 2941PhosphoserineBy similarity
    Modified residuei448 – 4481N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9JIH2.
    PaxDbiQ9JIH2.
    PRIDEiQ9JIH2.

    PTM databases

    PhosphoSiteiQ9JIH2.

    Expressioni

    Tissue specificityi

    Widely expressed at low levels. Highest in the developing neural tube and adult testes.

    Gene expression databases

    BgeeiQ9JIH2.
    GenevestigatoriQ9JIH2.

    Interactioni

    Subunit structurei

    Does not interact with TPR By similarity. Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi201817. 2 interactions.
    DIPiDIP-46130N.
    IntActiQ9JIH2. 2 interactions.
    MINTiMINT-4104287.

    Structurei

    Secondary structure

    1
    466
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni12 – 143
    Helixi32 – 354

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2C1MX-ray2.20B1-46[»]
    ProteinModelPortaliQ9JIH2.
    SMRiQ9JIH2. Positions 1-46, 349-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9JIH2.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati76 – 7721
    Repeati112 – 11322
    Repeati225 – 22623
    Repeati271 – 27224
    Repeati301 – 30225
    Domaini333 – 466134RanBD1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni76 – 3022275 X 2 AA repeats of F-GAdd
    BLAST
    Regioni143 – 20563Binding to CDKN1BAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi55 – 9036Gly-richAdd
    BLAST
    Compositional biasi266 – 32964Ser-richAdd
    BLAST

    Domaini

    Contains FG repeats.

    Sequence similaritiesi

    Contains 1 RanBD1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG255247.
    GeneTreeiENSGT00440000035348.
    HOVERGENiHBG052697.
    InParanoidiQ6P1F4.
    KOiK14295.
    OMAiANIEQQH.
    OrthoDBiEOG7CRTR9.
    TreeFamiTF106504.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR015007. NUP2/50/61.
    IPR011993. PH_like_dom.
    IPR000156. Ran_bind_dom.
    [Graphical view]
    PfamiPF08911. NUP50. 1 hit.
    PF00638. Ran_BP1. 1 hit.
    [Graphical view]
    SMARTiSM00160. RanBD. 1 hit.
    [Graphical view]
    PROSITEiPS50196. RANBD1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JIH2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKRVAEKEL TDRNWDEEDE VEEMGTFSVA SEEVMKNRAV KKAKRRNVGF    50
    ESDSGGAFKG FKGLVVPSGG GGFSGFGGSG GKPLEGLTNG NSTDNATPFS 100
    NVKTAAEPKA AFGSFAVNGP TTLVDKKISS PKCNNSNQPP SSGPASSTAC 150
    PGNAYHKQLA GLNCSVRDWI VKHVNTNPLC DLTPIFKDYE RYLATIEKQL 200
    ENGGGSSSES QTDRATAGME PPSLFGSTKL QQESPFSFHG NKAEDTSEKV 250
    EFTAEKKSDA AQGATSASFS FGKKIESSAL GSLSSGSLTG FSFSAGSSSL 300
    FGKDAAQSKA ASSLFSAKAS ESPAGGGSSE CRDGEEEEND EPPKVVVTEV 350
    KEEDAFYSKK CKLFYKKDNE FKEKGVGTLH LKPTATQKTQ LLVRADTNLG 400
    NILLNVLIAP NMPCTRTGKN NVLIVCVPNP PLDEKQPTLP ATMLIRVKTS 450
    EDADELHKIL LEKKDA 466
    Length:466
    Mass (Da):49,485
    Last modified:July 27, 2011 - v3
    Checksum:i8B9216CBB613661A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti127 – 1359KISSPKCNN → VSNPKTNGD in AAF70057. (PubMed:10811608)Curated
    Sequence conflicti158 – 1581Q → H in BAE40705. (PubMed:16141072)Curated
    Sequence conflicti314 – 3141L → P in AAF34721. (PubMed:10891500)Curated
    Sequence conflicti321 – 3211E → D in AAH60234. (PubMed:16141072)Curated
    Sequence conflicti324 – 3241A → P in AAF34721. (PubMed:10891500)Curated
    Sequence conflicti439 – 4413LPA → IKV in BAB31500. (PubMed:16141072)Curated
    Sequence conflicti451 – 4511E → K in BAB31500. (PubMed:16141072)Curated
    Sequence conflicti462 – 4643EKK → QKT in BAB31500. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF229256 mRNA. Translation: AAF34721.1.
    AF251799 mRNA. Translation: AAF70057.1.
    AK018983 mRNA. Translation: BAB31500.1.
    AK087706 mRNA. Translation: BAC39975.1.
    AK133371 mRNA. Translation: BAE21620.1.
    AK133562 mRNA. Translation: BAE21727.1.
    AK141750 mRNA. Translation: BAE24822.1.
    AK151947 mRNA. Translation: BAE30821.1.
    AK152309 mRNA. Translation: BAE31115.1.
    AK161713 mRNA. Translation: BAE36546.1.
    AK165581 mRNA. Translation: BAE38272.1.
    AK168888 mRNA. Translation: BAE40705.1.
    CH466550 Genomic DNA. Translation: EDL04446.1.
    BC059239 mRNA. Translation: AAH59239.1.
    BC060234 mRNA. Translation: AAH60234.1.
    BC065102 mRNA. Translation: AAH65102.1.
    CCDSiCCDS27714.1.
    RefSeqiNP_057923.2. NM_016714.2.
    UniGeneiMm.28379.
    Mm.391486.

    Genome annotation databases

    EnsembliENSMUST00000165443; ENSMUSP00000131457; ENSMUSG00000016619.
    GeneIDi18141.
    KEGGimmu:18141.
    UCSCiuc007xcq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF229256 mRNA. Translation: AAF34721.1 .
    AF251799 mRNA. Translation: AAF70057.1 .
    AK018983 mRNA. Translation: BAB31500.1 .
    AK087706 mRNA. Translation: BAC39975.1 .
    AK133371 mRNA. Translation: BAE21620.1 .
    AK133562 mRNA. Translation: BAE21727.1 .
    AK141750 mRNA. Translation: BAE24822.1 .
    AK151947 mRNA. Translation: BAE30821.1 .
    AK152309 mRNA. Translation: BAE31115.1 .
    AK161713 mRNA. Translation: BAE36546.1 .
    AK165581 mRNA. Translation: BAE38272.1 .
    AK168888 mRNA. Translation: BAE40705.1 .
    CH466550 Genomic DNA. Translation: EDL04446.1 .
    BC059239 mRNA. Translation: AAH59239.1 .
    BC060234 mRNA. Translation: AAH60234.1 .
    BC065102 mRNA. Translation: AAH65102.1 .
    CCDSi CCDS27714.1.
    RefSeqi NP_057923.2. NM_016714.2.
    UniGenei Mm.28379.
    Mm.391486.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2C1M X-ray 2.20 B 1-46 [» ]
    ProteinModelPortali Q9JIH2.
    SMRi Q9JIH2. Positions 1-46, 349-466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201817. 2 interactions.
    DIPi DIP-46130N.
    IntActi Q9JIH2. 2 interactions.
    MINTi MINT-4104287.

    PTM databases

    PhosphoSitei Q9JIH2.

    Proteomic databases

    MaxQBi Q9JIH2.
    PaxDbi Q9JIH2.
    PRIDEi Q9JIH2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000165443 ; ENSMUSP00000131457 ; ENSMUSG00000016619 .
    GeneIDi 18141.
    KEGGi mmu:18141.
    UCSCi uc007xcq.1. mouse.

    Organism-specific databases

    CTDi 10762.
    MGIi MGI:1351502. Nup50.

    Phylogenomic databases

    eggNOGi NOG255247.
    GeneTreei ENSGT00440000035348.
    HOVERGENi HBG052697.
    InParanoidi Q6P1F4.
    KOi K14295.
    OMAi ANIEQQH.
    OrthoDBi EOG7CRTR9.
    TreeFami TF106504.

    Enzyme and pathway databases

    Reactomei REACT_196650. Nuclear Pore Complex (NPC) Disassembly.
    REACT_204192. Regulation of Glucokinase by Glucokinase Regulatory Protein.

    Miscellaneous databases

    ChiTaRSi NUP50. mouse.
    EvolutionaryTracei Q9JIH2.
    NextBioi 293388.
    PROi Q9JIH2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JIH2.
    Genevestigatori Q9JIH2.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR015007. NUP2/50/61.
    IPR011993. PH_like_dom.
    IPR000156. Ran_bind_dom.
    [Graphical view ]
    Pfami PF08911. NUP50. 1 hit.
    PF00638. Ran_BP1. 1 hit.
    [Graphical view ]
    SMARTi SM00160. RanBD. 1 hit.
    [Graphical view ]
    PROSITEi PS50196. RANBD1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization and targeted disruption of murine nup50, a p27(Kip1)-interacting component of the nuclear pore complex."
      Smitherman M., Lee K., Swanger J., Kapur R., Clurman B.E.
      Mol. Cell. Biol. 20:5631-5642(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo.
    2. "Cyclin E-mediated elimination of p27 requires its interaction with the nuclear pore-associated protein mNPAP60."
      Mueller D., Thieke K., Buergin A., Dickmanns A., Eilers M.
      EMBO J. 19:2168-2180(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Amnion, Bone marrow, Ovary, Pituitary, Testis and Thymus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-8; LYS-126 AND LYS-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    6. "Nup50/Npap60 function in nuclear protein import complex disassembly and importin recycling."
      Matsuura Y., Stewart M.
      EMBO J. 24:3681-3689(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-46 IN COMPLEX WITH KPNA2, FUNCTION.

    Entry informationi

    Entry nameiNUP50_MOUSE
    AccessioniPrimary (citable) accession number: Q9JIH2
    Secondary accession number(s): Q3TG43
    , Q3TN17, Q6P1F4, Q6PAL4, Q8C2Y6, Q9CU02, Q9JK85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3