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Q9JIH2

- NUP50_MOUSE

UniProt

Q9JIH2 - NUP50_MOUSE

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Protein

Nuclear pore complex protein Nup50

Gene

Nup50

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.1 Publication

GO - Biological processi

  1. intracellular transport Source: InterPro
  2. mRNA transport Source: UniProtKB-KW
  3. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiREACT_196650. Nuclear Pore Complex (NPC) Disassembly.
REACT_204192. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_270446. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore complex protein Nup50
Alternative name(s):
50 kDa nucleoporin
Nuclear pore-associated protein 60 kDa-like
Nucleoporin Nup50
Gene namesi
Name:Nup50
Synonyms:Npap60
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1351502. Nup50.

Subcellular locationi

Nucleusnuclear pore complex. Nucleus membrane By similarity; Peripheral membrane protein By similarity; Nucleoplasmic side By similarity
Note: Localizes to the nucleoplasmic fibrils of the nuclear pore complex. Dissociates from the NPC structure early during prophase of mitosis. Associates to the newly formed nuclear membrane during telophase (By similarity).By similarity

GO - Cellular componenti

  1. nuclear membrane Source: UniProtKB
  2. nuclear pore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Nup50 are the cause of neural tube defects, exencephaly and intrauterine growth retardation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Nuclear pore complex protein Nup50PRO_0000204869Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81N6-acetyllysine1 Publication
Modified residuei82 – 821N6-acetyllysineBy similarity
Modified residuei126 – 1261N6-acetyllysine1 Publication
Modified residuei246 – 2461PhosphothreonineBy similarity
Modified residuei294 – 2941PhosphoserineBy similarity
Modified residuei448 – 4481N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9JIH2.
PaxDbiQ9JIH2.
PRIDEiQ9JIH2.

PTM databases

PhosphoSiteiQ9JIH2.

Expressioni

Tissue specificityi

Widely expressed at low levels. Highest in the developing neural tube and adult testes.

Gene expression databases

BgeeiQ9JIH2.
GenevestigatoriQ9JIH2.

Interactioni

Subunit structurei

Does not interact with TPR (By similarity). Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself.By similarity1 Publication

Protein-protein interaction databases

BioGridi201817. 2 interactions.
DIPiDIP-46130N.
IntActiQ9JIH2. 2 interactions.
MINTiMINT-4104287.

Structurei

Secondary structure

1
466
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni12 – 143Combined sources
Helixi32 – 354Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2C1MX-ray2.20B1-46[»]
ProteinModelPortaliQ9JIH2.
SMRiQ9JIH2. Positions 1-46, 349-466.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JIH2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati76 – 7721
Repeati112 – 11322
Repeati225 – 22623
Repeati271 – 27224
Repeati301 – 30225
Domaini333 – 466134RanBD1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 3022275 X 2 AA repeats of F-GAdd
BLAST
Regioni143 – 20563Binding to CDKN1BAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi55 – 9036Gly-richAdd
BLAST
Compositional biasi266 – 32964Ser-richAdd
BLAST

Domaini

Contains FG repeats.

Sequence similaritiesi

Contains 1 RanBD1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG255247.
GeneTreeiENSGT00440000035348.
HOVERGENiHBG052697.
InParanoidiQ9JIH2.
KOiK14295.
OMAiANIEQQH.
OrthoDBiEOG7CRTR9.
TreeFamiTF106504.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR015007. NUP2/50/61.
IPR011993. PH_like_dom.
IPR000156. Ran_bind_dom.
[Graphical view]
PfamiPF08911. NUP50. 1 hit.
PF00638. Ran_BP1. 1 hit.
[Graphical view]
SMARTiSM00160. RanBD. 1 hit.
[Graphical view]
PROSITEiPS50196. RANBD1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIH2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKRVAEKEL TDRNWDEEDE VEEMGTFSVA SEEVMKNRAV KKAKRRNVGF
60 70 80 90 100
ESDSGGAFKG FKGLVVPSGG GGFSGFGGSG GKPLEGLTNG NSTDNATPFS
110 120 130 140 150
NVKTAAEPKA AFGSFAVNGP TTLVDKKISS PKCNNSNQPP SSGPASSTAC
160 170 180 190 200
PGNAYHKQLA GLNCSVRDWI VKHVNTNPLC DLTPIFKDYE RYLATIEKQL
210 220 230 240 250
ENGGGSSSES QTDRATAGME PPSLFGSTKL QQESPFSFHG NKAEDTSEKV
260 270 280 290 300
EFTAEKKSDA AQGATSASFS FGKKIESSAL GSLSSGSLTG FSFSAGSSSL
310 320 330 340 350
FGKDAAQSKA ASSLFSAKAS ESPAGGGSSE CRDGEEEEND EPPKVVVTEV
360 370 380 390 400
KEEDAFYSKK CKLFYKKDNE FKEKGVGTLH LKPTATQKTQ LLVRADTNLG
410 420 430 440 450
NILLNVLIAP NMPCTRTGKN NVLIVCVPNP PLDEKQPTLP ATMLIRVKTS
460
EDADELHKIL LEKKDA
Length:466
Mass (Da):49,485
Last modified:July 27, 2011 - v3
Checksum:i8B9216CBB613661A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti127 – 1359KISSPKCNN → VSNPKTNGD in AAF70057. (PubMed:10811608)Curated
Sequence conflicti158 – 1581Q → H in BAE40705. (PubMed:16141072)Curated
Sequence conflicti314 – 3141L → P in AAF34721. (PubMed:10891500)Curated
Sequence conflicti321 – 3211E → D in AAH60234. (PubMed:16141072)Curated
Sequence conflicti324 – 3241A → P in AAF34721. (PubMed:10891500)Curated
Sequence conflicti439 – 4413LPA → IKV in BAB31500. (PubMed:16141072)Curated
Sequence conflicti451 – 4511E → K in BAB31500. (PubMed:16141072)Curated
Sequence conflicti462 – 4643EKK → QKT in BAB31500. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229256 mRNA. Translation: AAF34721.1.
AF251799 mRNA. Translation: AAF70057.1.
AK018983 mRNA. Translation: BAB31500.1.
AK087706 mRNA. Translation: BAC39975.1.
AK133371 mRNA. Translation: BAE21620.1.
AK133562 mRNA. Translation: BAE21727.1.
AK141750 mRNA. Translation: BAE24822.1.
AK151947 mRNA. Translation: BAE30821.1.
AK152309 mRNA. Translation: BAE31115.1.
AK161713 mRNA. Translation: BAE36546.1.
AK165581 mRNA. Translation: BAE38272.1.
AK168888 mRNA. Translation: BAE40705.1.
CH466550 Genomic DNA. Translation: EDL04446.1.
BC059239 mRNA. Translation: AAH59239.1.
BC060234 mRNA. Translation: AAH60234.1.
BC065102 mRNA. Translation: AAH65102.1.
CCDSiCCDS27714.1.
RefSeqiNP_057923.2. NM_016714.2.
UniGeneiMm.28379.
Mm.391486.

Genome annotation databases

EnsembliENSMUST00000165443; ENSMUSP00000131457; ENSMUSG00000016619.
GeneIDi18141.
KEGGimmu:18141.
UCSCiuc007xcq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229256 mRNA. Translation: AAF34721.1 .
AF251799 mRNA. Translation: AAF70057.1 .
AK018983 mRNA. Translation: BAB31500.1 .
AK087706 mRNA. Translation: BAC39975.1 .
AK133371 mRNA. Translation: BAE21620.1 .
AK133562 mRNA. Translation: BAE21727.1 .
AK141750 mRNA. Translation: BAE24822.1 .
AK151947 mRNA. Translation: BAE30821.1 .
AK152309 mRNA. Translation: BAE31115.1 .
AK161713 mRNA. Translation: BAE36546.1 .
AK165581 mRNA. Translation: BAE38272.1 .
AK168888 mRNA. Translation: BAE40705.1 .
CH466550 Genomic DNA. Translation: EDL04446.1 .
BC059239 mRNA. Translation: AAH59239.1 .
BC060234 mRNA. Translation: AAH60234.1 .
BC065102 mRNA. Translation: AAH65102.1 .
CCDSi CCDS27714.1.
RefSeqi NP_057923.2. NM_016714.2.
UniGenei Mm.28379.
Mm.391486.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2C1M X-ray 2.20 B 1-46 [» ]
ProteinModelPortali Q9JIH2.
SMRi Q9JIH2. Positions 1-46, 349-466.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201817. 2 interactions.
DIPi DIP-46130N.
IntActi Q9JIH2. 2 interactions.
MINTi MINT-4104287.

PTM databases

PhosphoSitei Q9JIH2.

Proteomic databases

MaxQBi Q9JIH2.
PaxDbi Q9JIH2.
PRIDEi Q9JIH2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000165443 ; ENSMUSP00000131457 ; ENSMUSG00000016619 .
GeneIDi 18141.
KEGGi mmu:18141.
UCSCi uc007xcq.1. mouse.

Organism-specific databases

CTDi 10762.
MGIi MGI:1351502. Nup50.

Phylogenomic databases

eggNOGi NOG255247.
GeneTreei ENSGT00440000035348.
HOVERGENi HBG052697.
InParanoidi Q9JIH2.
KOi K14295.
OMAi ANIEQQH.
OrthoDBi EOG7CRTR9.
TreeFami TF106504.

Enzyme and pathway databases

Reactomei REACT_196650. Nuclear Pore Complex (NPC) Disassembly.
REACT_204192. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_270446. Transcriptional regulation by small RNAs.

Miscellaneous databases

ChiTaRSi Nup50. mouse.
EvolutionaryTracei Q9JIH2.
NextBioi 293388.
PROi Q9JIH2.
SOURCEi Search...

Gene expression databases

Bgeei Q9JIH2.
Genevestigatori Q9JIH2.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR015007. NUP2/50/61.
IPR011993. PH_like_dom.
IPR000156. Ran_bind_dom.
[Graphical view ]
Pfami PF08911. NUP50. 1 hit.
PF00638. Ran_BP1. 1 hit.
[Graphical view ]
SMARTi SM00160. RanBD. 1 hit.
[Graphical view ]
PROSITEi PS50196. RANBD1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and targeted disruption of murine nup50, a p27(Kip1)-interacting component of the nuclear pore complex."
    Smitherman M., Lee K., Swanger J., Kapur R., Clurman B.E.
    Mol. Cell. Biol. 20:5631-5642(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "Cyclin E-mediated elimination of p27 requires its interaction with the nuclear pore-associated protein mNPAP60."
    Mueller D., Thieke K., Buergin A., Dickmanns A., Eilers M.
    EMBO J. 19:2168-2180(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Amnion, Bone marrow, Ovary, Pituitary, Testis and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-8; LYS-126 AND LYS-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  6. "Nup50/Npap60 function in nuclear protein import complex disassembly and importin recycling."
    Matsuura Y., Stewart M.
    EMBO J. 24:3681-3689(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-46 IN COMPLEX WITH KPNA2, FUNCTION.

Entry informationi

Entry nameiNUP50_MOUSE
AccessioniPrimary (citable) accession number: Q9JIH2
Secondary accession number(s): Q3TG43
, Q3TN17, Q6P1F4, Q6PAL4, Q8C2Y6, Q9CU02, Q9JK85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3