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Protein

Nuclear pore complex protein Nup50

Gene

Nup50

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:10811608). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling (PubMed:16222336). Interacts with regulatory proteins of cell cycle progression including CDKN1B (PubMed:10891500, PubMed:10811608). This interaction is required for correct intracellular transport and degradation of CDKN1B (PubMed:10811608).3 Publications

GO - Molecular functioni

GO - Biological processi

  • mRNA transport Source: UniProtKB-KW
  • neural tube formation Source: MGI
  • protein import into nucleus Source: GO_Central

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-170822 Regulation of Glucokinase by Glucokinase Regulatory Protein
R-MMU-191859 snRNP Assembly
R-MMU-3108214 SUMOylation of DNA damage response and repair proteins
R-MMU-3301854 Nuclear Pore Complex (NPC) Disassembly
R-MMU-3371453 Regulation of HSF1-mediated heat shock response
R-MMU-4551638 SUMOylation of chromatin organization proteins
R-MMU-4615885 SUMOylation of DNA replication proteins
R-MMU-5578749 Transcriptional regulation by small RNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore complex protein Nup50
Alternative name(s):
50 kDa nucleoporin
Nuclear pore-associated protein 60 kDa-like
Nucleoporin Nup50
Gene namesi
Name:Nup50
Synonyms:Npap60
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1351502 Nup50

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Disruption phenotypei

NUP50 targeted disruption results in a complex phenotype characterized by neural tube defects, exencephaly, intrauterine growth retardation, and late embryonic lethality.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048691 – 466Nuclear pore complex protein Nup50Add BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8N6-acetyllysineCombined sources1
Modified residuei52PhosphoserineBy similarity1
Modified residuei82N6-acetyllysineBy similarity1
Modified residuei126N6-acetyllysineCombined sources1
Modified residuei208PhosphoserineBy similarity1
Modified residuei234PhosphoserineCombined sources1
Modified residuei246PhosphothreonineBy similarity1
Modified residuei268PhosphoserineBy similarity1
Modified residuei294PhosphoserineBy similarity1
Cross-linki351Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei448N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9JIH2
MaxQBiQ9JIH2
PaxDbiQ9JIH2
PeptideAtlasiQ9JIH2
PRIDEiQ9JIH2

PTM databases

iPTMnetiQ9JIH2
PhosphoSitePlusiQ9JIH2

Expressioni

Tissue specificityi

Widely expressed at low levels. Highest in the developing neural tube and adult testes.

Gene expression databases

BgeeiENSMUSG00000016619
GenevisibleiQ9JIH2 MM

Interactioni

Subunit structurei

Does not interact with TPR (By similarity). Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201817, 3 interactors
DIPiDIP-46130N
IntActiQ9JIH2, 3 interactors
MINTiQ9JIH2
STRINGi10090.ENSMUSP00000131457

Structurei

Secondary structure

1466
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni12 – 14Combined sources3
Helixi32 – 35Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C1MX-ray2.20B1-46[»]
ProteinModelPortaliQ9JIH2
SMRiQ9JIH2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JIH2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati76 – 7712
Repeati112 – 11322
Repeati225 – 22632
Repeati271 – 27242
Repeati301 – 30252
Domaini333 – 466RanBD1PROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 3025 X 2 AA repeats of F-GAdd BLAST227
Regioni143 – 205Binding to CDKN1BAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 90Gly-richAdd BLAST36
Compositional biasi266 – 329Ser-richAdd BLAST64

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2724 Eukaryota
ENOG410YPHB LUCA
GeneTreeiENSGT00440000035348
HOVERGENiHBG052697
InParanoidiQ9JIH2
KOiK14295
OMAiTFLFHGN
OrthoDBiEOG091G0HML
TreeFamiTF106504

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR015007 NUP2/50/61
IPR011993 PH-like_dom_sf
IPR000156 Ran_bind_dom
PfamiView protein in Pfam
PF08911 NUP50, 1 hit
PF00638 Ran_BP1, 1 hit
SMARTiView protein in SMART
SM00160 RanBD, 1 hit
PROSITEiView protein in PROSITE
PS50196 RANBD1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JIH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRVAEKEL TDRNWDEEDE VEEMGTFSVA SEEVMKNRAV KKAKRRNVGF
60 70 80 90 100
ESDSGGAFKG FKGLVVPSGG GGFSGFGGSG GKPLEGLTNG NSTDNATPFS
110 120 130 140 150
NVKTAAEPKA AFGSFAVNGP TTLVDKKISS PKCNNSNQPP SSGPASSTAC
160 170 180 190 200
PGNAYHKQLA GLNCSVRDWI VKHVNTNPLC DLTPIFKDYE RYLATIEKQL
210 220 230 240 250
ENGGGSSSES QTDRATAGME PPSLFGSTKL QQESPFSFHG NKAEDTSEKV
260 270 280 290 300
EFTAEKKSDA AQGATSASFS FGKKIESSAL GSLSSGSLTG FSFSAGSSSL
310 320 330 340 350
FGKDAAQSKA ASSLFSAKAS ESPAGGGSSE CRDGEEEEND EPPKVVVTEV
360 370 380 390 400
KEEDAFYSKK CKLFYKKDNE FKEKGVGTLH LKPTATQKTQ LLVRADTNLG
410 420 430 440 450
NILLNVLIAP NMPCTRTGKN NVLIVCVPNP PLDEKQPTLP ATMLIRVKTS
460
EDADELHKIL LEKKDA
Length:466
Mass (Da):49,485
Last modified:July 27, 2011 - v3
Checksum:i8B9216CBB613661A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127 – 135KISSPKCNN → VSNPKTNGD in AAF70057 (PubMed:10811608).Curated9
Sequence conflicti158Q → H in BAE40705 (PubMed:16141072).Curated1
Sequence conflicti314L → P in AAF34721 (PubMed:10891500).Curated1
Sequence conflicti321E → D in AAH60234 (PubMed:16141072).Curated1
Sequence conflicti324A → P in AAF34721 (PubMed:10891500).Curated1
Sequence conflicti439 – 441LPA → IKV in BAB31500 (PubMed:16141072).Curated3
Sequence conflicti451E → K in BAB31500 (PubMed:16141072).Curated1
Sequence conflicti462 – 464EKK → QKT in BAB31500 (PubMed:16141072).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229256 mRNA Translation: AAF34721.1
AF251799 mRNA Translation: AAF70057.1
AK018983 mRNA Translation: BAB31500.1
AK087706 mRNA Translation: BAC39975.1
AK133371 mRNA Translation: BAE21620.1
AK133562 mRNA Translation: BAE21727.1
AK141750 mRNA Translation: BAE24822.1
AK151947 mRNA Translation: BAE30821.1
AK152309 mRNA Translation: BAE31115.1
AK161713 mRNA Translation: BAE36546.1
AK165581 mRNA Translation: BAE38272.1
AK168888 mRNA Translation: BAE40705.1
CH466550 Genomic DNA Translation: EDL04446.1
BC059239 mRNA Translation: AAH59239.1
BC060234 mRNA Translation: AAH60234.1
BC065102 mRNA Translation: AAH65102.1
CCDSiCCDS27714.1
RefSeqiNP_057923.2, NM_016714.2
UniGeneiMm.28379
Mm.391486

Genome annotation databases

EnsembliENSMUST00000165443; ENSMUSP00000131457; ENSMUSG00000016619
ENSMUST00000230411; ENSMUSP00000155656; ENSMUSG00000016619
GeneIDi18141
KEGGimmu:18141
UCSCiuc007xcq.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNUP50_MOUSE
AccessioniPrimary (citable) accession number: Q9JIH2
Secondary accession number(s): Q3TG43
, Q3TN17, Q6P1F4, Q6PAL4, Q8C2Y6, Q9CU02, Q9JK85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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