Q9JIG1 (FUT9_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-(1,3)-fucosyltransferase
EC=2.4.1.-

Alternative name(s):
Fucosyltransferase 9
Fucosyltransferase IX
Short name=Fuc-TIX
Short name=FucT-IX
Galactoside 3-L-fucosyltransferase

Gene names

Name:

FUT9

Organism

Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transfers a fucose to lacto-N-neotetraose but not to either alpha2,3-sialyl lacto-N-neotetraose or lacto-N-tetraose. Can catalyze the last step in the biosynthesis of Lewis antigen, the addition of a fucose to precursor polysaccharides By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Sequence similarities	Belongs to the glycosyltransferase 10 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	fucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Alpha-(1,3)-fucosyltransferase		PRO_0000221117
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	33 – 359	327	Lumenal Potential
Amino acid modifications
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9JIG1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 9D5CD8BFF07EA902	FASTA	359	42,071
	10 20 30 40 50 60 MTSTSKGILR PFLIVCVILA CFMACLLIYI KPTNSWVFSP MESASSVLKM KNFFSTKTDY 70 80 90 100 110 120 FNETTILVWV WPFGQTFDLT SCQAMFNIQG CHLTTDRSLY NKSHAVLIHH RDISWDLTNL 130 140 150 160 170 180 PQQARPPFQK WIWMNLESPT HTPQKSGIEH LFNLTLTYRR DSDIQVPYGF LTVSTNPFVF 190 200 210 220 230 240 EVPSKEKLVC WVVSNWNPEH ARVKYYNELS KSIEIHTYGQ AFGEYVNEKN LIPTISTCKF 250 260 270 280 290 300 YLSFENSIHK DYITEKLYNA FLAGSVPVVL GPSRENYENY IPADSFIHVE DYNSPSELAK 310 320 330 340 350 YLKEVDKNNK LYLSYFNWRK DFTVNLPRFW ESHACLACDH VKRHQEYKSV GNLEKWFWN

References

[1]	"Alpha(1,3)fucosyltransferases expressed by the gain-of-function Chinese hamster ovary glycosylation mutants LEC12, LEC29, and LEC30." Patnaik S.K., Zhang A., Shi S., Stanley P. Arch. Biochem. Biophys. 375:322-332(2000) [PubMed: 10700388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Patnaik S.K., Potvin B., Stanley P. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF230460 mRNA. Translation: AAF82412.1. AY628212 mRNA. Translation: AAT47342.1. AY628213 mRNA. Translation: AAT47343.1.
RefSeq	NP_001230953.1. NM_001244024.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT10. Glycosyltransferase Family 10.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100689036.
Phylogenomic databases
HOVERGEN	HBG000274.
Family and domain databases
InterPro	IPR001503. Glyco_trans_10. [Graphical view]
PANTHER	PTHR11929. Glyco_trans_10. 1 hit.
Pfam	PF00852. Glyco_transf_10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT9_CRIGR

Accession

Primary (citable) accession number: Q9JIG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program