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Protein

Peroxisomal trans-2-enoyl-CoA reductase

Gene

PECR

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Participates in chain elongation of fatty acids. Has no 2,4-dienoyl-CoA reductase activity.

Catalytic activityi

Acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH.1 Publication

Kineticsi

  1. KM=11 µM for decenoyl-CoA1 Publication
  2. KM=53 µM for NADPH1 Publication

    Pathway: fatty acid biosynthesis

    This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
    View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei178 – 1781Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi23 – 4725NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    • trans-2-enoyl-CoA reductase (NADPH) activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BRENDAi1.3.1.38. 1225.
    SABIO-RKQ9JIF5.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal trans-2-enoyl-CoA reductase (EC:1.3.1.38)
    Gene namesi
    Name:PECR
    OrganismiCavia porcellus (Guinea pig)
    Taxonomic identifieri10141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
    ProteomesiUP000005447 Componenti: Unassembled WGS sequence

    Subcellular locationi

    • Peroxisome 1 Publication

    GO - Cellular componenti

    • intracellular membrane-bounded organelle Source: UniProtKB
    • mitochondrion Source: UniProtKB
    • peroxisomal membrane Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 302301Peroxisomal trans-2-enoyl-CoA reductasePRO_0000054739Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylglycineBy similarity
    Modified residuei32 – 321N6-succinyllysineBy similarity
    Modified residuei49 – 491PhosphoserineBy similarity
    Modified residuei83 – 831N6-acetyllysineBy similarity
    Modified residuei178 – 1781PhosphotyrosineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Expressioni

    Tissue specificityi

    Expressed in liver.1 Publication

    Interactioni

    Subunit structurei

    Interacts with PEX5, probably required to target it into peroxisomes.By similarity

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000010538.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JIF5.
    SMRiQ9JIF5. Positions 8-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi300 – 3023Microbody targeting signalBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    GeneTreeiENSGT00760000118868.
    HOVERGENiHBG105268.
    InParanoidiQ9JIF5.
    OMAiMLADIWG.
    OrthoDBiEOG7Q5HFK.
    TreeFamiTF315256.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9JIF5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGSWTKCQSC LAPGLLQNRA AIVTGGGTGI GKAIAKELLH LGCNVVIASR
    60 70 80 90 100
    KFDRLRAAAE ELKATLPPSN KAEVTPIQCN IRKEEEVNNL MKSTLALYGK
    110 120 130 140 150
    IDFLVNNGGG QFWSSPEHIS SKGWHAVIET NLTGTFYMCK AAYNSWMKEH
    160 170 180 190 200
    GGAIVNIIIL LNGQPFVAHS GAARGGVYNL TKSLALGWAR SGIRINCVAP
    210 220 230 240 250
    GTVYSQTAMD NYGDMGKTLF ADAFQKIPAK RLGVPEEVSS LVCFLLSPAA
    260 270 280 290 300
    SFITGQLVNV DGGQSLYCQN HDIPDHDNWP EGVGDLSTVK KMKESFKQKA

    KL
    Length:302
    Mass (Da):32,529
    Last modified:October 1, 2000 - v1
    Checksum:i9C1430D90D4C07C2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF232010 mRNA. Translation: AAF69799.1.
    RefSeqiXP_003462124.1. XM_003462076.2.

    Genome annotation databases

    EnsembliENSCPOT00000011831; ENSCPOP00000010538; ENSCPOG00000011717.
    GeneIDi100725791.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF232010 mRNA. Translation: AAF69799.1.
    RefSeqiXP_003462124.1. XM_003462076.2.

    3D structure databases

    ProteinModelPortaliQ9JIF5.
    SMRiQ9JIF5. Positions 8-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000010538.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSCPOT00000011831; ENSCPOP00000010538; ENSCPOG00000011717.
    GeneIDi100725791.

    Organism-specific databases

    CTDi55825.

    Phylogenomic databases

    eggNOGiCOG1028.
    GeneTreeiENSGT00760000118868.
    HOVERGENiHBG105268.
    InParanoidiQ9JIF5.
    OMAiMLADIWG.
    OrthoDBiEOG7Q5HFK.
    TreeFamiTF315256.

    Enzyme and pathway databases

    UniPathwayiUPA00094.
    BRENDAi1.3.1.38. 1225.
    SABIO-RKQ9JIF5.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    ProtoNetiSearch...

    Publicationsi

    1. "Molecular cloning and expression of mammalian peroxisomal trans-2-enoyl-coenzyme A reductase cDNAs."
      Das A.K., Uhler M.D., Hajra A.K.
      J. Biol. Chem. 275:24333-24340(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 92-118, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Liver.

    Entry informationi

    Entry nameiPECR_CAVPO
    AccessioniPrimary (citable) accession number: Q9JIF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: October 1, 2000
    Last modified: May 27, 2015
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.