Q9JIF5 (PECR_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal trans-2-enoyl-CoA reductase
EC=1.3.1.38

Gene names

Name:

PECR

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in chain elongation of fatty acids. Has no 2,4-dienoyl-CoA reductase activity.
Catalytic activity	Acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH. Ref.1
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Interacts with PEX5, probably required to target it into peroxisomes By similarity.
Subcellular location	Peroxisome Ref.1.
Tissue specificity	Expressed in liver. Ref.1
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Biophysicochemical properties	Kinetic parameters: KM=11 µM for decenoyl-CoA Ref.1 KM=53 µM for NADPH

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Peroxisome
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro trans-2-enoyl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Peroxisomal trans-2-enoyl-CoA reductase		PRO_0000054739
Regions
	Nucleotide binding	23 – 47	25	NADP By similarity
	Motif	300 – 302	3	Microbody targeting signal By similarity
Sites
	Active site	178	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	178	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JIF5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 9C1430D90D4C07C2	FASTA	302	32,529
	10 20 30 40 50 60 MGSWTKCQSC LAPGLLQNRA AIVTGGGTGI GKAIAKELLH LGCNVVIASR KFDRLRAAAE 70 80 90 100 110 120 ELKATLPPSN KAEVTPIQCN IRKEEEVNNL MKSTLALYGK IDFLVNNGGG QFWSSPEHIS 130 140 150 160 170 180 SKGWHAVIET NLTGTFYMCK AAYNSWMKEH GGAIVNIIIL LNGQPFVAHS GAARGGVYNL 190 200 210 220 230 240 TKSLALGWAR SGIRINCVAP GTVYSQTAMD NYGDMGKTLF ADAFQKIPAK RLGVPEEVSS 250 260 270 280 290 300 LVCFLLSPAA SFITGQLVNV DGGQSLYCQN HDIPDHDNWP EGVGDLSTVK KMKESFKQKA KL

References

[1]

"Molecular cloning and expression of mammalian peroxisomal trans-2-enoyl-coenzyme A reductase cDNAs." Das A.K., Uhler M.D., Hajra A.K. J. Biol. Chem. 275:24333-24340(2000) [PubMed: 10811639] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 92-118, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF232010 mRNA. Translation: AAF69799.1.
RefSeq	XP_003462124.1. XM_003462076.1.
3D structure databases
ProteinModelPortal	Q9JIF5.
SMR	Q9JIF5. Positions 8-302.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9JIF5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000011831; ENSCPOP00000010538; ENSCPOG00000011717.
GeneID	100725791.
Phylogenomic databases
eggNOG	roNOG16912.
GeneTree	ENSGT00600000084005.
HOVERGEN	HBG105268.
InParanoid	Q9JIF5.
OMA	FYMCKAX.
OrthoDB	EOG415GF8.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PECR_CAVPO

Accession

Primary (citable) accession number: Q9JIF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program