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Protein

Solute carrier family 2, facilitated glucose transporter member 8

Gene

Slc2a8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Insulin-regulated facilitative glucose transporter. Binds cytochalasin B in a glucose-inhibitable manner. Seems to be a dual-specific sugar transporter as it is inhibitable by fructose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei394 – 3941MonosaccharideBy similarity

GO - Molecular functioni

GO - Biological processi

  • fructose transport Source: MGI
  • galactose transport Source: MGI
  • glucose import Source: GO_Central
  • glucose metabolic process Source: MGI
  • glucose transport Source: MGI
  • insulin receptor signaling pathway Source: MGI
  • male meiosis I Source: Ensembl
  • response to hypoxia Source: MGI
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-428790. Facilitative Na+-independent glucose transporters.

Protein family/group databases

TCDBi2.A.1.1.46. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 2, facilitated glucose transporter member 8
Alternative name(s):
Glucose transporter type 8
Short name:
GLUT-8
Glucose transporter type X1
Gene namesi
Name:Slc2a8
Synonyms:Glut8, GlutX1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1860103. Slc2a8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence analysisAdd
BLAST
Transmembranei26 – 4621Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini47 – 7024ExtracellularSequence analysisAdd
BLAST
Transmembranei71 – 9121Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini92 – 965CytoplasmicSequence analysis
Transmembranei97 – 11721Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini118 – 12710ExtracellularSequence analysis
Transmembranei128 – 14821Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini149 – 1568CytoplasmicSequence analysis
Transmembranei157 – 17721Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini178 – 1825ExtracellularSequence analysis
Transmembranei183 – 20321Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini204 – 25754CytoplasmicSequence analysisAdd
BLAST
Transmembranei258 – 27821Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini279 – 29315ExtracellularSequence analysisAdd
BLAST
Transmembranei294 – 31421Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini315 – 3206CytoplasmicSequence analysis
Transmembranei321 – 34121Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini342 – 36726ExtracellularSequence analysisAdd
BLAST
Transmembranei368 – 38821Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini389 – 40416CytoplasmicSequence analysisAdd
BLAST
Transmembranei405 – 42521Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini426 – 43813ExtracellularSequence analysisAdd
BLAST
Transmembranei439 – 45921Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini460 – 47718CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi12 – 132LL → AA: Abolishes interaction with AP2B1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Solute carrier family 2, facilitated glucose transporter member 8PRO_0000050376Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi350 – 3501N-linked (GlcNAc...)By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9JIF3.
PaxDbiQ9JIF3.
PRIDEiQ9JIF3.

PTM databases

PhosphoSiteiQ9JIF3.

Expressioni

Tissue specificityi

Highest level of expression in placenta and testis. Highly expressed in adult and pubertal testis, but not prepubertal testis. Lower levels of expression in brain, liver, heart, kidney, fat and skeletal muscle.

Developmental stagei

High expression in blastocysts.

Inductioni

Inhibited under glucose deprivation.

Gene expression databases

BgeeiENSMUSG00000026791.
ExpressionAtlasiQ9JIF3. baseline and differential.
GenevisibleiQ9JIF3. MM.

Interactioni

Subunit structurei

Interacts with AP2B1.1 Publication

Protein-protein interaction databases

IntActiQ9JIF3. 7 interactions.
MINTiMINT-7035216.
STRINGi10090.ENSMUSP00000028129.

Structurei

3D structure databases

ProteinModelPortaliQ9JIF3.
SMRiQ9JIF3. Positions 26-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni268 – 2747Monosaccharide bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 132Dileucine internalization motifSequence analysis

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0254. Eukaryota.
ENOG410XNQK. LUCA.
GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202868.
HOVERGENiHBG104335.
InParanoidiQ9JIF3.
KOiK08145.
OMAiAIMFYAN.
OrthoDBiEOG091G1600.
TreeFamiTF325324.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 2 hits.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPEDPQETQ PLLRPPEART PRGRRVFLAS FAAALGPLSF GFALGYSSPA
60 70 80 90 100
IPSLRRTAPP ALRLGDNAAS WFGAVVTLGA AAGGILGGWL LDRAGRKLSL
110 120 130 140 150
LLCTVPFVTG FAVITAARDV WMLLGGRLLT GLACGVASLV APVYISEIAY
160 170 180 190 200
PAVRGLLGSC VQLMVVTGIL LAYVAGWVLE WRWLAVLGCV PPTLMLLLMC
210 220 230 240 250
YMPETPRFLL TQHQYQEAMA ALRFLWGSEE GWEEPPVGAE HQGFQLALLR
260 270 280 290 300
RPGIYKPLII GISLMVFQQL SGVNAIMFYA NSIFEEAKFK DSSLASVTVG
310 320 330 340 350
IIQVLFTAVA ALIMDRAGRR LLLALSGVIM VFSMSAFGTY FKLTQSLPSN
360 370 380 390 400
SSHVGLVPIA AEPVDVQVGL AWLAVGSMCL FIAGFAVGWG PIPWLLMSEI
410 420 430 440 450
FPLHVKGVAT GICVLTNWFM AFLVTKEFSS VMEMLRPYGA FWLTAAFCAL
460 470
SVLFTLTVVP ETKGRTLEQV TAHFEGR
Length:477
Mass (Da):51,508
Last modified:July 27, 2011 - v2
Checksum:i59A985B52FADF478
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391S → N in CAB75719 (PubMed:10671487).Curated
Sequence conflicti94 – 941A → S in CAB75719 (PubMed:10671487).Curated
Sequence conflicti94 – 941A → S in AAF78366 (PubMed:10860996).Curated
Sequence conflicti429 – 4291S → N in CAB75719 (PubMed:10671487).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245936 mRNA. Translation: CAB75719.1.
Y17802 mRNA. Translation: CAB89815.1.
AF232061 mRNA. Translation: AAF78366.1.
AJ413951 Genomic DNA. Translation: CAC88690.1.
AY856043 mRNA. Translation: AAX51785.1.
AK081806 mRNA. Translation: BAC38338.1.
AK170319 mRNA. Translation: BAE41714.1.
AL731852 Genomic DNA. Translation: CAM22323.1.
CH466542 Genomic DNA. Translation: EDL08590.1.
BC090993 mRNA. Translation: AAH90993.1.
CCDSiCCDS15937.1.
RefSeqiNP_062361.1. NM_019488.4.
UniGeneiMm.305754.

Genome annotation databases

EnsembliENSMUST00000028129; ENSMUSP00000028129; ENSMUSG00000026791.
GeneIDi56017.
KEGGimmu:56017.
UCSCiuc008jhf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245936 mRNA. Translation: CAB75719.1.
Y17802 mRNA. Translation: CAB89815.1.
AF232061 mRNA. Translation: AAF78366.1.
AJ413951 Genomic DNA. Translation: CAC88690.1.
AY856043 mRNA. Translation: AAX51785.1.
AK081806 mRNA. Translation: BAC38338.1.
AK170319 mRNA. Translation: BAE41714.1.
AL731852 Genomic DNA. Translation: CAM22323.1.
CH466542 Genomic DNA. Translation: EDL08590.1.
BC090993 mRNA. Translation: AAH90993.1.
CCDSiCCDS15937.1.
RefSeqiNP_062361.1. NM_019488.4.
UniGeneiMm.305754.

3D structure databases

ProteinModelPortaliQ9JIF3.
SMRiQ9JIF3. Positions 26-477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JIF3. 7 interactions.
MINTiMINT-7035216.
STRINGi10090.ENSMUSP00000028129.

Protein family/group databases

TCDBi2.A.1.1.46. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteiQ9JIF3.

Proteomic databases

MaxQBiQ9JIF3.
PaxDbiQ9JIF3.
PRIDEiQ9JIF3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028129; ENSMUSP00000028129; ENSMUSG00000026791.
GeneIDi56017.
KEGGimmu:56017.
UCSCiuc008jhf.1. mouse.

Organism-specific databases

CTDi29988.
MGIiMGI:1860103. Slc2a8.

Phylogenomic databases

eggNOGiKOG0254. Eukaryota.
ENOG410XNQK. LUCA.
GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202868.
HOVERGENiHBG104335.
InParanoidiQ9JIF3.
KOiK08145.
OMAiAIMFYAN.
OrthoDBiEOG091G1600.
TreeFamiTF325324.

Enzyme and pathway databases

ReactomeiR-MMU-428790. Facilitative Na+-independent glucose transporters.

Miscellaneous databases

PROiQ9JIF3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026791.
ExpressionAtlasiQ9JIF3. baseline and differential.
GenevisibleiQ9JIF3. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 2 hits.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGTR8_MOUSE
AccessioniPrimary (citable) accession number: Q9JIF3
Secondary accession number(s): Q5BKP8, Q9JJP4, Q9JJZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.