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Protein

Programmed cell death protein 4

Gene

Pdcd4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Programmed cell death protein 4
Alternative name(s):
Death up-regulated gene protein
Gene namesi
Name:Pdcd4
Synonyms:Dug
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620816. Pdcd4.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002565221 – 469Programmed cell death protein 4Add BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei25PhosphoserineCombined sources1
Modified residuei67Phosphoserine; by PKB and RPS6KB1By similarity1
Modified residuei68PhosphoserineBy similarity1
Modified residuei71PhosphoserineBy similarity1
Modified residuei76PhosphoserineCombined sources1
Modified residuei78PhosphoserineBy similarity1
Modified residuei94PhosphoserineBy similarity1
Modified residuei152PhosphotyrosineBy similarity1
Modified residuei313PhosphoserineBy similarity1
Modified residuei317PhosphoserineBy similarity1
Modified residuei457PhosphoserineCombined sources1

Post-translational modificationi

Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation (By similarity).By similarity
Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9JID1.
PRIDEiQ9JID1.

PTM databases

iPTMnetiQ9JID1.
PhosphoSitePlusiQ9JID1.

Expressioni

Inductioni

Up-regulated by apoptotic inducers.1 Publication

Gene expression databases

BgeeiENSRNOG00000014779.
ExpressionAtlasiQ9JID1. baseline and differential.
GenevisibleiQ9JID1. RN.

Interactioni

Subunit structurei

Interacts (via MI domains) with EIF4A2 (By similarity). Interacts (via MI domains) with EIF4A1 (via N-terminal domain). Heterotrimer with EIF4A1; one molecule of PDCD4 binds two molecules of EIF4A1. Interacts with EIF4G1. May form a complex with EIF4A1 and EIF4G1. The interaction between PDCD4 and EIF4A1 interferes with the interaction between EIF4A1 and EIF4G. When phosphorylated, interacts with BTRC and FBXW11 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9JID1. 1 interactor.
STRINGi10116.ENSRNOP00000020155.

Structurei

3D structure databases

ProteinModelPortaliQ9JID1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini163 – 284MI 1PROSITE-ProRule annotationAdd BLAST122
Domaini326 – 449MI 2PROSITE-ProRule annotationAdd BLAST124

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 64Nuclear localization signalSequence analysis7
Motifi70 – 76PhosphodegronBy similarity7
Motifi448 – 454Nuclear localization signalSequence analysis7

Domaini

Binds EIF4A1 via both MI domains.

Sequence similaritiesi

Belongs to the PDCD4 family.Curated
Contains 2 MI domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0403. Eukaryota.
ENOG410XQZZ. LUCA.
GeneTreeiENSGT00390000015948.
HOGENOMiHOG000261612.
HOVERGENiHBG052841.
InParanoidiQ9JID1.
KOiK16865.
OMAiDGYKGTV.
OrthoDBiEOG091G07EZ.
PhylomeDBiQ9JID1.
TreeFamiTF323207.

Family and domain databases

Gene3Di1.25.40.180. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
[Graphical view]
PfamiPF02847. MA3. 2 hits.
[Graphical view]
SMARTiSM00544. MA3. 2 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51366. MI. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JID1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVENEQILN VNPTDPDNLS DSLFSGDEEN AGTEEIKNEI NGNWISASTI
60 70 80 90 100
NEARINAKAK RRLRKNSSRD SGRGDSVSDN GSEAVRSGVA VPTSPKGRLL
110 120 130 140 150
DRRSRSGKGR GLPKKGGAGG KGVWGTPGQV YDVEEVDVKD PNYDDDQENC
160 170 180 190 200
VYETVVLPLD ETAFEKTLTP IIQEYFEHGD TNEVAEMLRD LNLGEMKSGV
210 220 230 240 250
PVLAVSLALE GKASHREMTS KLLSDLCGTV MSTNDVEKSF DKLLKDLPEL
260 270 280 290 300
ALDTPRAPQL VGQFIARAVG DGILCNTYID SYKGTVDCVQ ARAALDKATV
310 320 330 340 350
LLSMSKGGKR KDSVWGSGGG QQPVNHLVKE IDMLLKEYLL SGDMSEAEHC
360 370 380 390 400
LKELEVPHFH HELVYEAIVM VLESTGESAF KMMLDLLKSL WKSSTITIDQ
410 420 430 440 450
MKRGYERIYN EIPDINLDVP HSYSVLERFV EECFQAGIIS KQLRDLCPSR
460
GRKRFVSEGD GGRLKPESY
Length:469
Mass (Da):51,736
Last modified:October 1, 2002 - v2
Checksum:i16D712EA267F9EEC
GO
Isoform 2 (identifier: Q9JID1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     367-403: Missing.

Show »
Length:432
Mass (Da):47,551
Checksum:i800052C62E66E429
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021356367 – 403Missing in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239739 mRNA. Translation: AAF73961.2.
RefSeqiNP_071601.2. NM_022265.2. [Q9JID1-1]
XP_006231675.1. XM_006231613.3. [Q9JID1-1]
XP_006231676.1. XM_006231614.3. [Q9JID1-1]
XP_006231677.1. XM_006231615.3. [Q9JID1-1]
XP_006231678.1. XM_006231616.3. [Q9JID1-1]
XP_017445137.1. XM_017589648.1. [Q9JID1-1]
UniGeneiRn.122017.

Genome annotation databases

EnsembliENSRNOT00000020155; ENSRNOP00000020155; ENSRNOG00000014779. [Q9JID1-1]
GeneIDi64031.
KEGGirno:64031.
UCSCiRGD:620816. rat. [Q9JID1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239739 mRNA. Translation: AAF73961.2.
RefSeqiNP_071601.2. NM_022265.2. [Q9JID1-1]
XP_006231675.1. XM_006231613.3. [Q9JID1-1]
XP_006231676.1. XM_006231614.3. [Q9JID1-1]
XP_006231677.1. XM_006231615.3. [Q9JID1-1]
XP_006231678.1. XM_006231616.3. [Q9JID1-1]
XP_017445137.1. XM_017589648.1. [Q9JID1-1]
UniGeneiRn.122017.

3D structure databases

ProteinModelPortaliQ9JID1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JID1. 1 interactor.
STRINGi10116.ENSRNOP00000020155.

PTM databases

iPTMnetiQ9JID1.
PhosphoSitePlusiQ9JID1.

Proteomic databases

PaxDbiQ9JID1.
PRIDEiQ9JID1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020155; ENSRNOP00000020155; ENSRNOG00000014779. [Q9JID1-1]
GeneIDi64031.
KEGGirno:64031.
UCSCiRGD:620816. rat. [Q9JID1-1]

Organism-specific databases

CTDi27250.
RGDi620816. Pdcd4.

Phylogenomic databases

eggNOGiKOG0403. Eukaryota.
ENOG410XQZZ. LUCA.
GeneTreeiENSGT00390000015948.
HOGENOMiHOG000261612.
HOVERGENiHBG052841.
InParanoidiQ9JID1.
KOiK16865.
OMAiDGYKGTV.
OrthoDBiEOG091G07EZ.
PhylomeDBiQ9JID1.
TreeFamiTF323207.

Miscellaneous databases

PROiQ9JID1.

Gene expression databases

BgeeiENSRNOG00000014779.
ExpressionAtlasiQ9JID1. baseline and differential.
GenevisibleiQ9JID1. RN.

Family and domain databases

Gene3Di1.25.40.180. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
[Graphical view]
PfamiPF02847. MA3. 2 hits.
[Graphical view]
SMARTiSM00544. MA3. 2 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51366. MI. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDCD4_RAT
AccessioniPrimary (citable) accession number: Q9JID1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.