Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

General transcription factor IIH subunit 2

Gene

Gtf2h2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri292 – 30918C4-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_279019. Dual incision reaction in TC-NER.
REACT_282911. RNA Polymerase II Pre-transcription Events.
REACT_283211. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_289837. RNA Polymerase I Transcription Termination.
REACT_296099. RNA Polymerase I Promoter Escape.
REACT_296420. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_296832. Dual incision reaction in GG-NER.
REACT_302527. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_308057. Formation of the Early Elongation Complex.
REACT_314261. Formation of incision complex in GG-NER.
REACT_315215. RNA Polymerase II Transcription Elongation.
REACT_326102. Formation of RNA Pol II elongation complex.
REACT_329615. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_338828. mRNA Capping.
REACT_342690. RNA Polymerase II Transcription Initiation.
REACT_349121. RNA Polymerase II Promoter Escape.
REACT_350574. RNA Polymerase I Transcription Initiation.
REACT_354877. RNA Polymerase I Chain Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 2
Alternative name(s):
Basic transcription factor 2 44 kDa subunit
Short name:
BTF2 p44
General transcription factor IIH polypeptide 2
TFIIH basal transcription factor complex p44 subunit
Gene namesi
Name:Gtf2h2
Synonyms:Btf2p44
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1345669. Gtf2h2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396General transcription factor IIH subunit 2PRO_0000119249Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei95 – 951PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JIB4.
PaxDbiQ9JIB4.
PRIDEiQ9JIB4.

PTM databases

PhosphoSiteiQ9JIB4.

Expressioni

Gene expression databases

BgeeiQ9JIB4.
CleanExiMM_GTF2H2.
ExpressionAtlasiQ9JIB4. baseline and differential.
GenevisibleiQ9JIB4. MM.

Interactioni

Subunit structurei

One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with XPB, XPD, GTF2H1 and GTF2H3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi204782. 3 interactions.
IntActiQ9JIB4. 1 interaction.
STRINGi10090.ENSMUSP00000065228.

Structurei

3D structure databases

ProteinModelPortaliQ9JIB4.
SMRiQ9JIB4. Positions 329-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 236177VWFAPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi242 – 2454Poly-Ser

Sequence similaritiesi

Belongs to the GTF2H2 family.Curated
Contains 1 VWFA domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri292 – 30918C4-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5151.
GeneTreeiENSGT00490000043395.
HOGENOMiHOG000159415.
HOVERGENiHBG059468.
InParanoidiQ9JIB4.
KOiK03142.
OrthoDBiEOG7WQ7SP.
PhylomeDBiQ9JIB4.
TreeFamiTF314037.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR007198. Ssl1-like.
IPR004595. TFIIH_C1-like_dom.
IPR012170. TFIIH_SSL1/p44.
IPR002035. VWF_A.
IPR007087. Znf_C2H2.
[Graphical view]
PANTHERiPTHR12695:SF2. PTHR12695:SF2. 1 hit.
PfamiPF07975. C1_4. 1 hit.
PF04056. Ssl1. 1 hit.
[Graphical view]
PIRSFiPIRSF015919. TFIIH_SSL1. 1 hit.
SMARTiSM01047. C1_4. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
TIGRFAMsiTIGR00622. ssl1. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEEPERTKR WEGGYERTWE ILKEDETGSL KATIEDILFK AKRKRVFEHH
60 70 80 90 100
GQVRLGMMRH LYVVVDGSRT MEDQDLKPNR LTCTLKLLEY FVEEYFDQNP
110 120 130 140 150
ISQIGIIVTK SKRAEKLTEL SGNPRKHITS LKKAVDMTCH GEPSLYNSLS
160 170 180 190 200
MAMQTLKHMP GHTSREVLII FSSLTTCDPS NIYDLIKTLK TAKIRVSVIG
210 220 230 240 250
LSAEVRVCTV LARETGGTYH VILDETHYKE LLAHHVSPPP ASSSSECSLI
260 270 280 290 300
RMGFPQHTIA SLSDQDAKPS FSMAHLDNNS TEPGLTLGGY FCPQCRAKYC
310 320 330 340 350
ELPVECKICG LTLVSAPHLA RSYHHLFPLD AFQEISLEEY KGERFCYGCQ
360 370 380 390
GELKDQHVYV CTVCQNVFCV DCDVFVHDSL HCCPGCIHKI PTPSGI
Length:396
Mass (Da):44,687
Last modified:October 1, 2000 - v1
Checksum:i1BC57AFD464AA83F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242432 Genomic DNA. Translation: AAF82753.1.
CCDSiCCDS26730.1.
RefSeqiNP_071294.3. NM_022011.4.
UniGeneiMm.272533.

Genome annotation databases

EnsembliENSMUST00000066984; ENSMUSP00000065228; ENSMUSG00000021639.
GeneIDi23894.
KEGGimmu:23894.
UCSCiuc007rqu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242432 Genomic DNA. Translation: AAF82753.1.
CCDSiCCDS26730.1.
RefSeqiNP_071294.3. NM_022011.4.
UniGeneiMm.272533.

3D structure databases

ProteinModelPortaliQ9JIB4.
SMRiQ9JIB4. Positions 329-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204782. 3 interactions.
IntActiQ9JIB4. 1 interaction.
STRINGi10090.ENSMUSP00000065228.

PTM databases

PhosphoSiteiQ9JIB4.

Proteomic databases

MaxQBiQ9JIB4.
PaxDbiQ9JIB4.
PRIDEiQ9JIB4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066984; ENSMUSP00000065228; ENSMUSG00000021639.
GeneIDi23894.
KEGGimmu:23894.
UCSCiuc007rqu.2. mouse.

Organism-specific databases

CTDi2966.
MGIiMGI:1345669. Gtf2h2.

Phylogenomic databases

eggNOGiCOG5151.
GeneTreeiENSGT00490000043395.
HOGENOMiHOG000159415.
HOVERGENiHBG059468.
InParanoidiQ9JIB4.
KOiK03142.
OrthoDBiEOG7WQ7SP.
PhylomeDBiQ9JIB4.
TreeFamiTF314037.

Enzyme and pathway databases

ReactomeiREACT_279019. Dual incision reaction in TC-NER.
REACT_282911. RNA Polymerase II Pre-transcription Events.
REACT_283211. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_289837. RNA Polymerase I Transcription Termination.
REACT_296099. RNA Polymerase I Promoter Escape.
REACT_296420. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_296832. Dual incision reaction in GG-NER.
REACT_302527. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_308057. Formation of the Early Elongation Complex.
REACT_314261. Formation of incision complex in GG-NER.
REACT_315215. RNA Polymerase II Transcription Elongation.
REACT_326102. Formation of RNA Pol II elongation complex.
REACT_329615. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_338828. mRNA Capping.
REACT_342690. RNA Polymerase II Transcription Initiation.
REACT_349121. RNA Polymerase II Promoter Escape.
REACT_350574. RNA Polymerase I Transcription Initiation.
REACT_354877. RNA Polymerase I Chain Elongation.

Miscellaneous databases

ChiTaRSiGtf2h2. mouse.
NextBioi303657.
PROiQ9JIB4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JIB4.
CleanExiMM_GTF2H2.
ExpressionAtlasiQ9JIB4. baseline and differential.
GenevisibleiQ9JIB4. MM.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR007198. Ssl1-like.
IPR004595. TFIIH_C1-like_dom.
IPR012170. TFIIH_SSL1/p44.
IPR002035. VWF_A.
IPR007087. Znf_C2H2.
[Graphical view]
PANTHERiPTHR12695:SF2. PTHR12695:SF2. 1 hit.
PfamiPF07975. C1_4. 1 hit.
PF04056. Ssl1. 1 hit.
[Graphical view]
PIRSFiPIRSF015919. TFIIH_SSL1. 1 hit.
SMARTiSM01047. C1_4. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
TIGRFAMsiTIGR00622. ssl1. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 188-195, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiTF2H2_MOUSE
AccessioniPrimary (citable) accession number: Q9JIB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 1, 2000
Last modified: June 24, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.