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Protein

Sphingosine kinase 2

Gene

Sphk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.By similarity

Catalytic activityi

ATP + a sphingoid base = ADP + a sphingoid base 1-phosphate.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei212Proton donor/acceptorBy similarity1
Binding sitei217ATPPROSITE-ProRule annotation1
Binding sitei309SubstrateBy similarity1
Binding sitei316ATPPROSITE-ProRule annotation1
Binding sitei322ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi153 – 155ATPPROSITE-ProRule annotation3
Nucleotide bindingi185 – 189ATPPROSITE-ProRule annotation5
Nucleotide bindingi242 – 244ATPPROSITE-ProRule annotation3
Nucleotide bindingi586 – 588ATPPROSITE-ProRule annotation3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • D-erythro-sphingosine kinase activity Source: MGI
  • sphinganine kinase activity Source: UniProtKB
  • sphingosine-1-phosphate receptor activity Source: MGI

GO - Biological processi

  • blood vessel development Source: MGI
  • brain development Source: MGI
  • cell proliferation Source: UniProtKB
  • female pregnancy Source: Ensembl
  • negative regulation of apoptotic process Source: UniProtKB
  • positive regulation of cell proliferation Source: MGI
  • sphinganine-1-phosphate biosynthetic process Source: UniProtKB
  • sphingosine biosynthetic process Source: MGI
  • sphingosine metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.91. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Chemistry databases

SwissLipidsiSLP:000000113.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine kinase 2 (EC:2.7.1.91By similarity)
Short name:
SK 2
Short name:
SPK 2
Gene namesi
Name:Sphk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1861380. Sphk2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • lysosomal membrane Source: MGI
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075305.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001813591 – 617Sphingosine kinase 2Add BLAST617

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei358PhosphoserineCombined sources1
Modified residuei364PhosphoserineCombined sources1
Modified residuei377PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JIA7.
MaxQBiQ9JIA7.
PaxDbiQ9JIA7.
PRIDEiQ9JIA7.

PTM databases

iPTMnetiQ9JIA7.
PhosphoSitePlusiQ9JIA7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000057342.
CleanExiMM_SPHK2.
ExpressionAtlasiQ9JIA7. baseline and differential.
GenevisibleiQ9JIA7. MM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FynP396882EBI-985434,EBI-524514

Protein-protein interaction databases

IntActiQ9JIA7. 6 interactors.
MINTiMINT-4135335.
STRINGi10090.ENSMUSP00000072615.

Chemistry databases

BindingDBiQ9JIA7.

Structurei

3D structure databases

ProteinModelPortaliQ9JIA7.
SMRiQ9JIA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 290DAGKcPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 140Required for binding to sulfatide and phosphoinositides and for membrane localizationBy similarityAdd BLAST140
Regioni210 – 213Substrate bindingBy similarity4

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1116. Eukaryota.
COG1597. LUCA.
GeneTreeiENSGT00690000101761.
HOGENOMiHOG000111460.
HOVERGENiHBG054796.
InParanoidiQ9JIA7.
KOiK04718.
OMAiLMCLLRG.
OrthoDBiEOG091G06SM.
PhylomeDBiQ9JIA7.
TreeFamiTF354296.

Family and domain databases

InterProiIPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JIA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPPLLPVA ASTPILHGEF GSYPANGPRF ALTLTTQALH IQRLRPKPEA
60 70 80 90 100
RPRDGLVSLD EVSGCGTLQS RSPEDTAAYF CIYTYPRGRR GGRRRATRTF
110 120 130 140 150
RADGATTYEE NRAEAQRWAT ALTCLLRGVP LSGDQEITPE LLPRKPRLLI
160 170 180 190 200
LVNPFGGRGL AWQRCMDHVV PMISEAGLSF NLIQTERQNH ARELVQGLSL
210 220 230 240 250
SEWEGIVTVS GDGLLYEVLN GLLDRPDWED AVRMPIGVLP CGSGNALAGA
260 270 280 290 300
VNHHGGFEQV VGVDLLLNCS LLLCRGGSHP LDLLSVTLAS GSRCFSFLSV
310 320 330 340 350
AWGFLSDVDI HSERFRALGS ARFTLGAVLG LASLHTYRGR LSYLPATTEP
360 370 380 390 400
ALPIPGHSLP RAKSELVLAP APAPAATHSP LHRSVSDLPL PLPQPALVSP
410 420 430 440 450
GSPEPLPDLS LNGGGPELTG DWGGAGDAPL SPDPLLPSSP NALKTAQLSP
460 470 480 490 500
IAEGPPEMPA SSGFLPPTHS APEASTWGPV DHLLPPLGSP LPQDWVTIEG
510 520 530 540 550
EFVLMLGILP SHLCADLMAA PHARFDDGVV HLCWVRSGIS RAALLRILLA
560 570 580 590 600
MEHGNHFSLG CPHLGYAAAR AFRLEPLTPR GLLTVDGELV EYGPIQAQVH
610
PGLATLLTGP AGQKPQA
Length:617
Mass (Da):65,618
Last modified:March 27, 2002 - v2
Checksum:i40EE2C2C288BE26A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252N → S in AAF74125 (PubMed:10751414).Curated1
Sequence conflicti510P → T in AAF74125 (PubMed:10751414).Curated1
Sequence conflicti548L → F in AAF74125 (PubMed:10751414).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245448 mRNA. Translation: AAF74125.1.
AF415214 mRNA. Translation: AAL07500.1.
AK004951 mRNA. Translation: BAB23694.1.
BC006941 mRNA. Translation: AAH06941.1.
BC053737 mRNA. Translation: AAH53737.1.
CCDSiCCDS21261.1.
RefSeqiNP_001166032.1. NM_001172561.1.
NP_064395.2. NM_020011.5.
NP_975009.1. NM_203280.3.
XP_006541075.1. XM_006541012.3.
XP_006541076.1. XM_006541013.3.
UniGeneiMm.24222.
Mm.378235.

Genome annotation databases

EnsembliENSMUST00000072836; ENSMUSP00000072615; ENSMUSG00000057342.
ENSMUST00000107737; ENSMUSP00000103366; ENSMUSG00000057342.
ENSMUST00000210060; ENSMUSP00000147391; ENSMUSG00000057342.
GeneIDi56632.
KEGGimmu:56632.
UCSCiuc009gwt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245448 mRNA. Translation: AAF74125.1.
AF415214 mRNA. Translation: AAL07500.1.
AK004951 mRNA. Translation: BAB23694.1.
BC006941 mRNA. Translation: AAH06941.1.
BC053737 mRNA. Translation: AAH53737.1.
CCDSiCCDS21261.1.
RefSeqiNP_001166032.1. NM_001172561.1.
NP_064395.2. NM_020011.5.
NP_975009.1. NM_203280.3.
XP_006541075.1. XM_006541012.3.
XP_006541076.1. XM_006541013.3.
UniGeneiMm.24222.
Mm.378235.

3D structure databases

ProteinModelPortaliQ9JIA7.
SMRiQ9JIA7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JIA7. 6 interactors.
MINTiMINT-4135335.
STRINGi10090.ENSMUSP00000072615.

Chemistry databases

BindingDBiQ9JIA7.
ChEMBLiCHEMBL1075305.
SwissLipidsiSLP:000000113.

PTM databases

iPTMnetiQ9JIA7.
PhosphoSitePlusiQ9JIA7.

Proteomic databases

EPDiQ9JIA7.
MaxQBiQ9JIA7.
PaxDbiQ9JIA7.
PRIDEiQ9JIA7.

Protocols and materials databases

DNASUi56632.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072836; ENSMUSP00000072615; ENSMUSG00000057342.
ENSMUST00000107737; ENSMUSP00000103366; ENSMUSG00000057342.
ENSMUST00000210060; ENSMUSP00000147391; ENSMUSG00000057342.
GeneIDi56632.
KEGGimmu:56632.
UCSCiuc009gwt.2. mouse.

Organism-specific databases

CTDi56848.
MGIiMGI:1861380. Sphk2.

Phylogenomic databases

eggNOGiKOG1116. Eukaryota.
COG1597. LUCA.
GeneTreeiENSGT00690000101761.
HOGENOMiHOG000111460.
HOVERGENiHBG054796.
InParanoidiQ9JIA7.
KOiK04718.
OMAiLMCLLRG.
OrthoDBiEOG091G06SM.
PhylomeDBiQ9JIA7.
TreeFamiTF354296.

Enzyme and pathway databases

BRENDAi2.7.1.91. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

ChiTaRSiSphk2. mouse.
PROiQ9JIA7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057342.
CleanExiMM_SPHK2.
ExpressionAtlasiQ9JIA7. baseline and differential.
GenevisibleiQ9JIA7. MM.

Family and domain databases

InterProiIPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPHK2_MOUSE
AccessioniPrimary (citable) accession number: Q9JIA7
Secondary accession number(s): Q91VA9, Q9DBH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: March 27, 2002
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.