Reviewed, UniProtKB/Swiss-Prot Q9JIA3 (IKBB_RAT)

Names and origin

Protein names

Recommended name:
    NF-kappa-B inhibitor beta
      Short name=NF-kappa-BIB
Alternative name(s):
    I-kappa-B-beta
      Short name=IkappaBbeta
      Short name=IkB-beta
      Short name=IkB-B

Gene names

Name:	Nfkbib
Synonyms:	Ikbb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
Subunit structure	Interacts with COMMD1 and inhibitor kappa B-interacting Ras-like NKIRAS1 and NKIRAS2 By similarity. Interacts with THRB (via ligand-binding domain). Interacts with RELA and REL By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Post-translational modification	Phosphorylated; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation By similarity.
Sequence similarities	Belongs to the NF-kappa-B inhibitor family. Contains 6 ANK repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	ANK repeat Repeat
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cytoplasmic sequestering of NF-kappaB Inferred from mutant phenotype. Source: RGD
Cellular component	I-kappaB/NF-kappaB complex Inferred by curator. Source: RGD cytosol Inferred from direct assay. Source: RGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NF-kappaB binding Inferred by curator. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	NF-kappa-B inhibitor beta		PRO_0000067006
Regions
	Repeat	57 – 86	30	ANK 1
	Repeat	93 – 122	30	ANK 2
	Repeat	126 – 155	30	ANK 3
	Repeat	206 – 235	30	ANK 4
	Repeat	240 – 269	30	ANK 5
	Repeat	273 – 302	30	ANK 6
Amino acid modifications
	Modified residue	19	1	Phosphoserine By similarity
	Modified residue	23	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JIA3-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: B8602C815C72B1AB	FASTA	359	38,081
	10 20 30 40 50 60 MAGVACLGKT ADADEWCDSG LGSLGPDAAA PGGPGLVAEL SPELSWAPLV FGYVTEDGDT 70 80 90 100 110 120 ALHLAVIHQH EPFLDFLLGF SAGTEYLDLQ NDLGQTALHL AAILGEASTV EKLYAAGAGV 130 140 150 160 170 180 LVTERGGHTA LHLACRVRAH TCAYVLLQPR PSHPRDASDT YLTQSQDHTP DTSHAPVATD 190 200 210 220 230 240 PQPNPGNEEE LRDEDWRLQL EAENYDGHTP LHVAVIHKDA EMVQLLRDAG ADLNKPEPTC 250 260 270 280 290 300 GRTPLHLAVE GQAAGVLALL LKAGADPTAR MYGGRTPLGS ALLRPNPVLA RLLRAHGAPE 310 320 330 340 350 PEDKDDKLSP CSNSSSDSDS DNRDEGDEYD DIVVHSRRSQ NQPPPSPAAK PLPDDPNPA

References

[1]	"Inhibitory and stimulatory effects of lactacystin on expression of nitric oxide synthase type 2 in brain glial cells: the role of I kappa B-beta." Stasiolek M., Gavrilyuk V., Sharp A., Horvath P., Selmaj K., Feinstein D.L. J. Biol. Chem. 275:24847-24856(2000) [PubMed: 10827092] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF246634 mRNA. Translation: AAF64191.1.
IPI	IPI00200350.
UniGene	Rn.8395
3D structure databases
HSSP	HSSP built from PDB template 1OY3 based on UniProtKB Q60778.
SMR	Q9JIA3. Positions 50-303.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9JIA3.
PTM databases
PhosphoSite	Q9JIA3.
Genome annotation databases
Ensembl	ENSRNOT00000027212; ENSRNOP00000027212; ENSRNOG00000020063; Rattus norvegicus. [Genome view]
UCSC	NM_030867. rat.
Organism-specific databases
RGD	621887. Nfkbib.
Phylogenomic databases
HOVERGEN	Q9JIA3.
Gene expression databases
ArrayExpress	Q9JIA3.
Genevestigator	Q9JIA3.
GermOnline	ENSRNOG00000020063. Rattus norvegicus.
Family and domain databases
InterPro	IPR002110. ANK. IPR015681. NF_kB_inhbitor. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
PANTHER	PTHR18958:SF236. NF_kB_inhbitor. 1 hit.
Pfam	PF00023. Ank. 6 hits. [Graphical view]
SMART	SM00248. ANK. 6 hits. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IKBB_RAT

Accession

Primary (citable) accession number: Q9JIA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	October 1, 2000
Last modified:	October 13, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)