Q9JIA1 (LGI1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine-rich glioma-inactivated protein 1

Gene names

Name:

Lgi1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors. Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway By similarity.
Subunit structure	Oligomer By similarity. Interacts with KCNA1 within a complex containing KCNA1, KCNA4 and KCNAB1 By similarity. Part of a complex containing ADAM22, DLG4/PSD95 and CACNG2 (stargazin) By similarity. Can bind to ADAM11 and ADAM23. Ref.7
Subcellular location	Secreted. Cell junction › synapse By similarity. Note: Isoform 1 and isoform 2 are detected in the cytosolic and microsomal fractions respectively. Ref.5 Ref.6
Tissue specificity	Brain. Ref.6
Post-translational modification	Glycosylated By similarity.
Sequence similarities	Contains 7 EAR repeats. Contains 3 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.

Ontologies

Keywords
Cellular component	Cell junction Secreted Synapse
Coding sequence diversity	Alternative splicing
Domain	Leucine-rich repeat Repeat Signal
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	axon guidance Inferred from electronic annotation. Source: Compara positive regulation of cell growth Inferred from electronic annotation. Source: Compara positive regulation of synaptic transmission Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW extracellular space Inferred from direct assay Ref.5. Source: UniProtKB synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]

Isoform 1 (identifier: Q9JIA1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JIA1-2)

The sequence of this isoform is not available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 557	523	Leucine-rich glioma-inactivated protein 1		PRO_0000017706
Regions
	Domain	35 – 72	38	LRRNT
	Repeat	92 – 113	22	LRR 1
	Repeat	116 – 137	22	LRR 2
	Repeat	140 – 161	22	LRR 3
	Domain	173 – 223	51	LRRCT
	Repeat	224 – 267	44	EAR 1
	Repeat	270 – 313	44	EAR 2
	Repeat	316 – 364	49	EAR 3
	Repeat	365 – 415	51	EAR 4
	Repeat	418 – 462	45	EAR 5
	Repeat	463 – 506	44	EAR 6
	Repeat	509 – 552	44	EAR 7
Amino acid modifications
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Glycosylation	277	1	N-linked (GlcNAc...) Potential
	Glycosylation	422	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2692D53F13DEC431	FASTA	557	63,644
	10 20 30 40 50 60 MESESSRRMG NACIPLKRIA YFLCLFSVVL LTEGKKPAKP KCPAVCTCSK DNALCENARS 70 80 90 100 110 120 IPRTVPPDVI SLSFVRSGFT EISEGSFLFT PSLQLLLFTS NSFDVISDDA FIGLPHLEYL 130 140 150 160 170 180 FIENNNIKSI SRHTFRGLKS LIHLSLANNN LQTLPKDIFK GLDSLTNVDL RGNAFNCDCK 190 200 210 220 230 240 LKWLVEWLGH TNATVEDIYC EGPPEYKKRK INSLSPKDFD CIITEFAKSQ DLPYQSLSID 250 260 270 280 290 300 TFSYLNDEYV VIAQPFTGKC IFLEWDHVEK TFRNYDNITG TSTVVCKPIV IDTQLYVIVA 310 320 330 340 350 360 QLFGGSHIYK RDGFANKFIK IQDIEVLKIR KPNDIETFKI EDNWYFVVAD SSKAGFTTIY 370 380 390 400 410 420 KWNGNGFYSH QSLHAWYRDT DVEYLEIARP PLALRTPHLI LSSSSQRPVI YQWSKATQLF 430 440 450 460 470 480 TNQTDIPNME DVYAVKHFSV KGDVYICLTR FIGDSKVMKW GGSSFQDIQR MPSRGSMVFQ 490 500 510 520 530 540 PLQINNYQYA ILGSDYSFTQ VYNWDAEKAK FVKFQELNVQ APRSFTHVSI NKRNFLFASS 550 FKGNTQIYKH VIVDLSA
	Isoform 2 (Sequence not available).		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the promoter, genomic structure, and mouse ortholog of LGI1." Somerville R.P.T., Chernova O., Liu S., Shoshan Y., Cowell J.K. Mamm. Genome 11:622-627(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 161-171 AND 442-450, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[5]	"ADPEAF mutations reduce levels of secreted LGI1, a putative tumor suppressor protein linked to epilepsy." Senechal K.R., Thaller C., Noebels J.L. Hum. Mol. Genet. 14:1613-1620(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"The LGI1/epitempin gene encodes two protein isoforms differentially expressed in human brain." Furlan S., Roncaroli F., Forner F., Vitiello L., Calabria E., Piquer-Sirerol S., Valle G., Perez-Tur J., Michelucci R., Nobile C. J. Neurochem. 98:985-991(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"LGI1 and LGI4 bind to ADAM22, ADAM23 and ADAM11." Sagane K., Ishihama Y., Sugimoto H. Int. J. Biol. Sci. 4:387-396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ADAM11; ADAM22 AND ADAM23.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF246818 mRNA. Translation: AAF87077.1. AK045443 mRNA. Translation: BAC32370.1. BC066090 mRNA. Translation: AAH66090.1.
IPI	IPI00120302.
RefSeq	NP_064674.1. NM_020278.2.
UniGene	Mm.298251.
3D structure databases
ProteinModelPortal	Q9JIA1.
SMR	Q9JIA1. Positions 42-265.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9JIA1. 3 interactions.
STRING	10090.ENSMUSP00000084507.
PTM databases
PhosphoSite	Q9JIA1.
Proteomic databases
PaxDb	Q9JIA1.
PRIDE	Q9JIA1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000087252; ENSMUSP00000084507; ENSMUSG00000067242.
GeneID	56839.
KEGG	mmu:56839.
UCSC	uc008hji.1. mouse.
Organism-specific databases
CTD	9211.
MGI	MGI:1861691. Lgi1.
Phylogenomic databases
eggNOG	NOG304734.
GeneTree	ENSGT00390000016191.
HOGENOM	HOG000286015.
HOVERGEN	HBG036744.
InParanoid	Q9JIA1.
OMA	GFANKFI.
OrthoDB	EOG4PZJ6C.
Gene expression databases
ArrayExpress	Q9JIA1.
Bgee	Q9JIA1.
CleanEx	MM_LGI1.
Genevestigator	Q9JIA1.
GermOnline	ENSMUSG00000067242. Mus musculus.
Family and domain databases
InterPro	IPR000483. Cys-rich_flank_reg_C. IPR009039. EAR. IPR005492. EPTP. IPR003591. Leu-rich_rpt_typical-subtyp. IPR000372. LRR-contain_N. [Graphical view]
Pfam	PF03736. EPTP. 7 hits. [Graphical view]
SMART	SM00369. LRR_TYP. 2 hits. SM00082. LRRCT. 1 hit. SM00013. LRRNT. 1 hit. [Graphical view]
PROSITE	PS50912. EAR. 7 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	313387.
SOURCE	Search...

Entry information

Entry name

LGI1_MOUSE

Accession

Primary (citable) accession number: Q9JIA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program