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Protein

Sphingosine-1-phosphate phosphatase 1

Gene

Sgpp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically dephosphorylates sphingosine 1-phosphate (S1P), dihydro-S1P, and phyto-S1P. Does not act on ceramide 1-phoshate, lysophosphatidic acid or phosphatidic acid. Regulates the intracellular levels of the bioactive sphingolipid metabolite S1P that regulates diverse biological processes acting both as an extracellular receptor ligand or as an intracellular second messenger (PubMed:10859351, Ref. 2). Involved in efficient ceramide synthesis from exogenous sphingoid bases (PubMed:12235122, PubMed:17895250). Converts S1P to sphingosine, which is readily metabolized to ceramide via ceramide synthase. In concert with sphingosine kinase 2 (SphK2), recycles sphingosine into ceramide though a phosphorylation/dephosphorylation cycle (PubMed:17895250). Regulates intracellular ceramide levels, which in turn regulate apoptosis (PubMed:12235122). Via S1P levels, modulates resting tone, intracellular Ca2+ and myogenic vasoconstriction in resistance arteries. Also involved in unfolded protein response (UPR) and ER stress-induced autophagy via regulation of intracellular S1P levels (By similarity).By similarity4 Publications

Enzyme regulationi

Inhibited by NaF, sodium orthovanadate, propanolol, and N-ethylmaleimide.1 Publication

Kineticsi

  1. KM=38.5 µM for sphingosine 1-phosphate1 Publication
  1. Vmax=36.4 nmol/min/mg enzyme for sphingosine 1-phosphate1 Publication

pH dependencei

Optimum pH is 6-7.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei197Proton donorBy similarity1
Active sitei244NucleophileBy similarity1
Sitei248Stabilizes the active site histidine for nucleophilic attackBy similarity1

GO - Molecular functioni

  • sphingosine-1-phosphate phosphatase activity Source: MGI

GO - Biological processi

  • extrinsic apoptotic signaling pathway Source: MGI
  • intrinsic apoptotic signaling pathway Source: MGI
  • sphinganine-1-phosphate metabolic process Source: MGI
  • sphingolipid metabolic process Source: MGI
  • sphingosine metabolic process Source: MGI

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Chemistry databases

SwissLipidsiSLP:000000381.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine-1-phosphate phosphatase 1 (EC:3.1.3.-)
Short name:
SPP
Short name:
SPPase1
Short name:
mSPP1
Alternative name(s):
Sphingosine-1-phosphatase 1
Gene namesi
Name:Sgpp1
Synonyms:Spp1, Spph1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2135760. Sgpp1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Transmembranei246 – 266HelicalSequence analysisAdd BLAST21
Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
Transmembranei311 – 331HelicalSequence analysisAdd BLAST21
Transmembranei348 – 368HelicalSequence analysisAdd BLAST21
Transmembranei409 – 429HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001144781 – 430Sphingosine-1-phosphate phosphatase 1Add BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101PhosphoserineCombined sources1
Modified residuei103PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JI99.
MaxQBiQ9JI99.
PaxDbiQ9JI99.
PRIDEiQ9JI99.

PTM databases

iPTMnetiQ9JI99.
PhosphoSitePlusiQ9JI99.

Expressioni

Tissue specificityi

Highly expressed in liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000021054.
CleanExiMM_SGPP1.
MM_SPP1.
ExpressionAtlasiQ9JI99. baseline and differential.
GenevisibleiQ9JI99. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021450.

Structurei

3D structure databases

ProteinModelPortaliQ9JI99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni167 – 175Phosphatase sequence motif ICurated9
Regioni194 – 197Phosphatase sequence motif IICurated4
Regioni237 – 248Phosphatase sequence motif IIICuratedAdd BLAST12

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQK8. Eukaryota.
ENOG410Z1GR. LUCA.
GeneTreeiENSGT00390000017322.
HOGENOMiHOG000234228.
HOVERGENiHBG079185.
InParanoidiQ9JI99.
KOiK04716.
OMAiFYILFFP.
OrthoDBiEOG091G092E.
PhylomeDBiQ9JI99.
TreeFamiTF323419.

Family and domain databases

InterProiView protein in InterPro
IPR036938. P_Acid_Pase_2/haloperoxi_sf.
IPR000326. P_Acid_Pase_2/haloperoxidase.
PfamiView protein in Pfam
PF01569. PAP2. 1 hit.
SMARTiView protein in SMART
SM00014. acidPPc. 1 hit.
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JI99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLGQRLALL ASRLQEPQRV ASFQRLCGVE VPLSSPAADE DAETEVRGAP
60 70 80 90 100
GEPRRRGRQP GAEDSPAKAD CCGAPNGVRN GLAAEPGPTG PRRAGSQRRN
110 120 130 140 150
SLTGEEGELV KVSNLPLYYL FCLGTELGNE LFYILFFPFW IWNLDPFVGR
160 170 180 190 200
RLVIIWVLVM YLGQCTKDII RWPRPASPPV IKLEVFYNSE YSMPSTHAMS
210 220 230 240 250
GTAIPIAMFL LTYGRWQYPL IYGLILIPCW SSLVCLSRIY MGMHSILDVI
260 270 280 290 300
AGFLYTILIL IIFYPLVDLI DNFNQTYKYA PLIIIGLHLI LGIFSFTLDT
310 320 330 340 350
WSTSRGDTAE ILGSGAGIAC GSHAAYTLGL SLEPSLHMLP LAIPPLTVTL
360 370 380 390 400
FGKAILRIVL GMLLVLFVRD IMKKITIPLA CKLSSIPCHD IRQARQHMEV
410 420 430
ELPYRYITYG MVGFSITFLV PYVFSFIGIS
Length:430
Mass (Da):47,744
Last modified:October 1, 2000 - v1
Checksum:i503A6D8162C141C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247177 mRNA. Translation: AAF90052.1.
AF415215 mRNA. Translation: AAL07501.1.
BC037592 mRNA. Translation: AAH37592.1.
CCDSiCCDS25986.1.
RefSeqiNP_109675.1. NM_030750.3.
UniGeneiMm.280199.

Genome annotation databases

EnsembliENSMUST00000021450; ENSMUSP00000021450; ENSMUSG00000021054.
GeneIDi81535.
KEGGimmu:81535.
UCSCiuc007nxk.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiSGPP1_MOUSE
AccessioniPrimary (citable) accession number: Q9JI99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 1, 2000
Last modified: October 25, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families