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Q9JI91

- ACTN2_MOUSE

UniProt

Q9JI91 - ACTN2_MOUSE

Protein

Alpha-actinin-2

Gene

Actn2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi766 – 777121; possibly ancestralAdd
    BLAST
    Calcium bindingi802 – 813122; possibly ancestralAdd
    BLAST

    GO - Molecular functioni

    1. actin filament binding Source: MGI
    2. calcium ion binding Source: InterPro
    3. cytoskeletal protein binding Source: UniProtKB
    4. ligand-dependent nuclear receptor transcription coactivator activity Source: MGI
    5. LIM domain binding Source: MGI
    6. protein binding Source: UniProtKB
    7. protein binding, bridging Source: MGI
    8. protein homodimerization activity Source: UniProtKB
    9. thyroid hormone receptor coactivator activity Source: MGI

    GO - Biological processi

    1. focal adhesion assembly Source: Ensembl
    2. microspike assembly Source: Ensembl
    3. muscle contraction Source: MGI
    4. negative regulation of potassium ion transmembrane transporter activity Source: Ensembl
    5. negative regulation of potassium ion transport Source: Ensembl
    6. negative regulation of protein localization to cell surface Source: Ensembl
    7. positive regulation of potassium ion transmembrane transporter activity Source: Ensembl
    8. positive regulation of potassium ion transport Source: Ensembl
    9. positive regulation of receptor activity Source: GOC
    10. protein homotetramerization Source: Ensembl
    11. regulation of membrane potential Source: Ensembl
    12. regulation of RNA biosynthetic process Source: GOC

    Keywords - Ligandi

    Actin-binding, Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
    REACT_198248. CREB phosphorylation through the activation of CaMKII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-actinin-2
    Alternative name(s):
    Alpha-actinin skeletal muscle isoform 2
    F-actin cross-linking protein
    Gene namesi
    Name:Actn2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:109192. Actn2.

    Subcellular locationi

    CytoplasmmyofibrilsarcomereZ line 1 Publication
    Note: Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.

    GO - Cellular componenti

    1. filopodium Source: Ensembl
    2. focal adhesion Source: Ensembl
    3. sarcomere Source: MGI
    4. striated muscle thin filament Source: MGI
    5. Z disc Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 894894Alpha-actinin-2PRO_0000073436Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by FBXL22, leading to proteasomal degradation.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    MaxQBiQ9JI91.
    PaxDbiQ9JI91.
    PRIDEiQ9JI91.

    PTM databases

    PhosphoSiteiQ9JI91.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9JI91.
    CleanExiMM_ACTN2.
    GenevestigatoriQ9JI91.

    Interactioni

    Subunit structurei

    Homodimer; antiparallel. Also forms heterodimers with ACTN3. Interacts with ADAM12, MYOZ1, MYOZ2 and MYOZ3. Interacts via its C-terminal region with the LDB3 PDZ domain. Interacts with XIRP2. Interacts with DST (via N-terminus). Interacts with PARVB By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Camk2aP117983EBI-299169,EBI-400384
    Grin2bQ010974EBI-299169,EBI-400125

    Protein-protein interaction databases

    BioGridi197950. 7 interactions.
    IntActiQ9JI91. 10 interactions.
    MINTiMINT-97958.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JI91.
    SMRiQ9JI91. Positions 35-261, 274-894.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 254254Actin-bindingAdd
    BLAST
    Domaini38 – 142105CH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini151 – 254104CH 2PROSITE-ProRule annotationAdd
    BLAST
    Repeati281 – 391111Spectrin 1Add
    BLAST
    Repeati401 – 506106Spectrin 2Add
    BLAST
    Repeati516 – 627112Spectrin 3Add
    BLAST
    Repeati637 – 740104Spectrin 4Add
    BLAST
    Domaini753 – 78836EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini789 – 82436EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the alpha-actinin family.Curated
    Contains 1 actin-binding domain.Curated
    Contains 2 CH (calponin-homology) domains.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 4 spectrin repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5069.
    GeneTreeiENSGT00670000097825.
    HOGENOMiHOG000263418.
    HOVERGENiHBG050453.
    InParanoidiQ9JI91.
    KOiK05699.
    OMAiRAKWDKV.
    OrthoDBiEOG72C4ZJ.
    TreeFamiTF352676.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    1.10.418.10. 2 hits.
    InterProiIPR001589. Actinin_actin-bd_CS.
    IPR001715. CH-domain.
    IPR011992. EF-hand-dom_pair.
    IPR014837. EF-hand_Ca_insen.
    IPR002048. EF_hand_dom.
    IPR018159. Spectrin/alpha-actinin.
    IPR002017. Spectrin_repeat.
    [Graphical view]
    PfamiPF00307. CH. 2 hits.
    PF08726. EFhand_Ca_insen. 1 hit.
    PF00435. Spectrin. 4 hits.
    [Graphical view]
    SMARTiSM00033. CH. 2 hits.
    SM00054. EFh. 2 hits.
    SM00150. SPEC. 3 hits.
    [Graphical view]
    SUPFAMiSSF47576. SSF47576. 1 hit.
    PROSITEiPS00019. ACTININ_1. 1 hit.
    PS00020. ACTININ_2. 1 hit.
    PS50021. CH. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JI91-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNQIEPGVQY NYVYDEDEYM IQEEEWDRDL LLDPAWEKQQ RKTFTAWCNS    50
    HLRKAGTQIE NIEEDFRNGL KLMLLLEVIS GERLPKPDRG KMRFHKIANV 100
    NKALDYIASK GVKLVSIGAE EIVDGNVKMT LGMIWTIILR FAIQDISVEE 150
    TSAKEGLLLW CQRKTAPYRN VNIQNFHTSW KDGLGLCALI HRHRPDLIDY 200
    SKLNKDDPIG NINLAMEIAE KHLDIPKMLD AEDIVNTPKP DERAIMTYVS 250
    CFYHAFAGAE QAETAANRIC KVLAVNQENE RLMEEYERLA SELLEWIRRT 300
    IPWLENRTPE KTMQAMQKKL EDFRDYRRKH KPPKVQEKCQ LEINFNTLQT 350
    KLRISNRPAF MPSEGKMVSD IAGAWQRLEQ AEKGYEEWLL NEIRRLERLE 400
    HLAEKFRQKA STHETWAYGK EQILLQKDYE SASLTEVRAL LRKHEAFESD 450
    LAAHQDRVEQ IAAIAQELNE LDYHDAVNVN DRCQKICDQW DRLGTLTQKR 500
    REALERTEKL LETIDQLHLE FAKRAAPFNN WMEGAMEDLQ DMFIVHSIEE 550
    IQSLITAHEQ FKATLPEADG ERQSILAIQN EVEKVIQSYS IRISSSNPYS 600
    TVTMDELRNK WDKVKQLVPV RDQSLQEELA RQHANERLRR QFAAQANAIG 650
    PWIQNKMEEI ARSSIQITGA LEDQMNQLKQ YEHNIINYKN NIDKLEGDHQ 700
    LIQEALVFDN KHTNYTMEHI RVGWELLLTT IARTINEVET QILTRDAKGI 750
    TQEQMNEFRA SFNHFDRRKN GLMDHEDFRA CLISMGYDLG EAEFARIMTL 800
    VDPNGQGTVT FQSFIDFMTR ETADTDTAEQ VIASFRILAS DKPYILAEEL 850
    RRELPPDQAQ YCIKRMPPYS GPGSVPGALD YTAFSSALYG ESDL 894
    Length:894
    Mass (Da):103,834
    Last modified:October 3, 2012 - v2
    Checksum:i79FCDC9C85B3949C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti272 – 2721V → G in AAF76325. (PubMed:11440986)Curated
    Sequence conflicti358 – 3581P → A in AAF76325. (PubMed:11440986)Curated
    Sequence conflicti375 – 3751W → C in AAF76325. (PubMed:11440986)Curated
    Sequence conflicti705 – 7051A → G in AAF76325. (PubMed:11440986)Curated
    Sequence conflicti732 – 7321A → G in AAF76325. (PubMed:11440986)Curated
    Sequence conflicti753 – 7531E → K in AAF76325. (PubMed:11440986)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF248643 mRNA. Translation: AAF76325.1.
    AC154523 Genomic DNA. No translation available.
    CH466588 Genomic DNA. Translation: EDL32304.1.
    CCDSiCCDS26239.1.
    RefSeqiNP_150371.4. NM_033268.4.
    UniGeneiMm.37638.

    Genome annotation databases

    EnsembliENSMUST00000064204; ENSMUSP00000067708; ENSMUSG00000052374.
    ENSMUST00000168193; ENSMUSP00000129609; ENSMUSG00000052374.
    GeneIDi11472.
    KEGGimmu:11472.
    UCSCiuc007pli.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF248643 mRNA. Translation: AAF76325.1 .
    AC154523 Genomic DNA. No translation available.
    CH466588 Genomic DNA. Translation: EDL32304.1 .
    CCDSi CCDS26239.1.
    RefSeqi NP_150371.4. NM_033268.4.
    UniGenei Mm.37638.

    3D structure databases

    ProteinModelPortali Q9JI91.
    SMRi Q9JI91. Positions 35-261, 274-894.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 197950. 7 interactions.
    IntActi Q9JI91. 10 interactions.
    MINTi MINT-97958.

    PTM databases

    PhosphoSitei Q9JI91.

    Proteomic databases

    MaxQBi Q9JI91.
    PaxDbi Q9JI91.
    PRIDEi Q9JI91.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000064204 ; ENSMUSP00000067708 ; ENSMUSG00000052374 .
    ENSMUST00000168193 ; ENSMUSP00000129609 ; ENSMUSG00000052374 .
    GeneIDi 11472.
    KEGGi mmu:11472.
    UCSCi uc007pli.1. mouse.

    Organism-specific databases

    CTDi 88.
    MGIi MGI:109192. Actn2.

    Phylogenomic databases

    eggNOGi COG5069.
    GeneTreei ENSGT00670000097825.
    HOGENOMi HOG000263418.
    HOVERGENi HBG050453.
    InParanoidi Q9JI91.
    KOi K05699.
    OMAi RAKWDKV.
    OrthoDBi EOG72C4ZJ.
    TreeFami TF352676.

    Enzyme and pathway databases

    Reactomei REACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
    REACT_198248. CREB phosphorylation through the activation of CaMKII.

    Miscellaneous databases

    ChiTaRSi ACTN2. mouse.
    NextBioi 278808.
    PROi Q9JI91.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JI91.
    CleanExi MM_ACTN2.
    Genevestigatori Q9JI91.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    1.10.418.10. 2 hits.
    InterProi IPR001589. Actinin_actin-bd_CS.
    IPR001715. CH-domain.
    IPR011992. EF-hand-dom_pair.
    IPR014837. EF-hand_Ca_insen.
    IPR002048. EF_hand_dom.
    IPR018159. Spectrin/alpha-actinin.
    IPR002017. Spectrin_repeat.
    [Graphical view ]
    Pfami PF00307. CH. 2 hits.
    PF08726. EFhand_Ca_insen. 1 hit.
    PF00435. Spectrin. 4 hits.
    [Graphical view ]
    SMARTi SM00033. CH. 2 hits.
    SM00054. EFh. 2 hits.
    SM00150. SPEC. 3 hits.
    [Graphical view ]
    SUPFAMi SSF47576. SSF47576. 1 hit.
    PROSITEi PS00019. ACTININ_1. 1 hit.
    PS00020. ACTININ_2. 1 hit.
    PS50021. CH. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential expression of the actin-binding proteins, alpha-actinin-2 and -3, in different species: implications for the evolution of functional redundancy."
      Mills M., Yang N., Weinberger R., Vander Woude D.L., Beggs A.H., Easteal S., North K.N.
      Hum. Mol. Genet. 10:1335-1346(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skeletal muscle.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha-actinin-2, is required for myoblast fusion."
      Galliano M.-F., Huet C., Frygelius J., Polgren A., Wewer U.M., Engvall E.
      J. Biol. Chem. 275:13933-13939(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ADAM12.
    5. "BPAG1 isoform-b: complex distribution pattern in striated and heart muscle and association with plectin and alpha-actinin."
      Steiner-Champliaud M.F., Schneider Y., Favre B., Paulhe F., Praetzel-Wunder S., Faulkner G., Konieczny P., Raith M., Wiche G., Adebola A., Liem R.K., Langbein L., Sonnenberg A., Fontao L., Borradori L.
      Exp. Cell Res. 316:297-313(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiACTN2_MOUSE
    AccessioniPrimary (citable) accession number: Q9JI91
    Secondary accession number(s): G3UW84
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3