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Protein

Alpha-actinin-2

Gene

Actn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi766 – 7771; possibly ancestralAdd BLAST12
Calcium bindingi802 – 8132; possibly ancestralAdd BLAST12

GO - Molecular functioni

  • actin filament binding Source: MGI
  • calcium ion binding Source: InterPro
  • cytoskeletal protein binding Source: UniProtKB
  • FATZ binding Source: MGI
  • identical protein binding Source: MGI
  • ion channel binding Source: UniProtKB
  • ligand-dependent nuclear receptor transcription coactivator activity Source: MGI
  • LIM domain binding Source: MGI
  • phosphatidylinositol-4,5-bisphosphate binding Source: MGI
  • protein binding, bridging Source: MGI
  • protein dimerization activity Source: MGI
  • protein domain specific binding Source: MGI
  • protein homodimerization activity Source: MGI
  • thyroid hormone receptor coactivator activity Source: MGI
  • titin binding Source: MGI
  • titin Z domain binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionActin-binding
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-390522 Striated Muscle Contraction
R-MMU-438066 Unblocking of NMDA receptor, glutamate binding and activation
R-MMU-442729 CREB phosphorylation through the activation of CaMKII
R-MMU-442982 Ras activation upon Ca2+ influx through NMDA receptor
R-MMU-5673001 RAF/MAP kinase cascade

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-actinin-2
Alternative name(s):
Alpha-actinin skeletal muscle isoform 2
F-actin cross-linking protein
Gene namesi
Name:Actn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:109192 Actn2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000734361 – 894Alpha-actinin-2Add BLAST894

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei237PhosphothreonineCombined sources1

Post-translational modificationi

Ubiquitinated by FBXL22, leading to proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9JI91
PaxDbiQ9JI91
PeptideAtlasiQ9JI91
PRIDEiQ9JI91

PTM databases

iPTMnetiQ9JI91
PhosphoSitePlusiQ9JI91

Expressioni

Gene expression databases

BgeeiENSMUSG00000052374
CleanExiMM_ACTN2
ExpressionAtlasiQ9JI91 baseline and differential
GenevisibleiQ9JI91 MM

Interactioni

Subunit structurei

Homodimer; antiparallel. Also forms heterodimers with ACTN3. Interacts with ADAM12, MYOZ1, MYOZ2 and MYOZ3. Interacts via its C-terminal region with the LDB3 PDZ domain. Interacts with XIRP2. Interacts with DST (via N-terminus). Interacts with PARVB. Interacts with SYNPO2 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin filament binding Source: MGI
  • cytoskeletal protein binding Source: UniProtKB
  • FATZ binding Source: MGI
  • identical protein binding Source: MGI
  • ion channel binding Source: UniProtKB
  • LIM domain binding Source: MGI
  • protein binding, bridging Source: MGI
  • protein dimerization activity Source: MGI
  • protein domain specific binding Source: MGI
  • protein homodimerization activity Source: MGI
  • titin binding Source: MGI
  • titin Z domain binding Source: MGI

Protein-protein interaction databases

BioGridi197950, 7 interactors
CORUMiQ9JI91
IntActiQ9JI91, 11 interactors
MINTiQ9JI91
STRINGi10090.ENSMUSP00000067708

Structurei

3D structure databases

ProteinModelPortaliQ9JI91
SMRiQ9JI91
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 142Calponin-homology (CH) 1PROSITE-ProRule annotationAdd BLAST105
Domaini151 – 257Calponin-homology (CH) 2PROSITE-ProRule annotationAdd BLAST107
Repeati281 – 391Spectrin 1Add BLAST111
Repeati401 – 506Spectrin 2Add BLAST106
Repeati516 – 627Spectrin 3Add BLAST112
Repeati637 – 740Spectrin 4Add BLAST104
Domaini753 – 788EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini789 – 824EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 254Actin-bindingAdd BLAST254

Sequence similaritiesi

Belongs to the alpha-actinin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0035 Eukaryota
COG5069 LUCA
GeneTreeiENSGT00760000118813
HOGENOMiHOG000263418
HOVERGENiHBG050453
InParanoidiQ9JI91
KOiK21073
OMAiIQSYNIR
OrthoDBiEOG091G020R
TreeFamiTF352676

Family and domain databases

CDDicd00014 CH, 2 hits
cd00051 EFh, 1 hit
Gene3Di1.10.418.10, 2 hits
InterProiView protein in InterPro
IPR001589 Actinin_actin-bd_CS
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR011992 EF-hand-dom_pair
IPR014837 EF-hand_Ca_insen
IPR002048 EF_hand_dom
IPR018159 Spectrin/alpha-actinin
IPR002017 Spectrin_repeat
PfamiView protein in Pfam
PF00307 CH, 2 hits
PF08726 EFhand_Ca_insen, 1 hit
PF00435 Spectrin, 4 hits
SMARTiView protein in SMART
SM00033 CH, 2 hits
SM00054 EFh, 2 hits
SM00150 SPEC, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS00019 ACTININ_1, 1 hit
PS00020 ACTININ_2, 1 hit
PS50021 CH, 2 hits
PS50222 EF_HAND_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q9JI91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQIEPGVQY NYVYDEDEYM IQEEEWDRDL LLDPAWEKQQ RKTFTAWCNS
60 70 80 90 100
HLRKAGTQIE NIEEDFRNGL KLMLLLEVIS GERLPKPDRG KMRFHKIANV
110 120 130 140 150
NKALDYIASK GVKLVSIGAE EIVDGNVKMT LGMIWTIILR FAIQDISVEE
160 170 180 190 200
TSAKEGLLLW CQRKTAPYRN VNIQNFHTSW KDGLGLCALI HRHRPDLIDY
210 220 230 240 250
SKLNKDDPIG NINLAMEIAE KHLDIPKMLD AEDIVNTPKP DERAIMTYVS
260 270 280 290 300
CFYHAFAGAE QAETAANRIC KVLAVNQENE RLMEEYERLA SELLEWIRRT
310 320 330 340 350
IPWLENRTPE KTMQAMQKKL EDFRDYRRKH KPPKVQEKCQ LEINFNTLQT
360 370 380 390 400
KLRISNRPAF MPSEGKMVSD IAGAWQRLEQ AEKGYEEWLL NEIRRLERLE
410 420 430 440 450
HLAEKFRQKA STHETWAYGK EQILLQKDYE SASLTEVRAL LRKHEAFESD
460 470 480 490 500
LAAHQDRVEQ IAAIAQELNE LDYHDAVNVN DRCQKICDQW DRLGTLTQKR
510 520 530 540 550
REALERTEKL LETIDQLHLE FAKRAAPFNN WMEGAMEDLQ DMFIVHSIEE
560 570 580 590 600
IQSLITAHEQ FKATLPEADG ERQSILAIQN EVEKVIQSYS IRISSSNPYS
610 620 630 640 650
TVTMDELRNK WDKVKQLVPV RDQSLQEELA RQHANERLRR QFAAQANAIG
660 670 680 690 700
PWIQNKMEEI ARSSIQITGA LEDQMNQLKQ YEHNIINYKN NIDKLEGDHQ
710 720 730 740 750
LIQEALVFDN KHTNYTMEHI RVGWELLLTT IARTINEVET QILTRDAKGI
760 770 780 790 800
TQEQMNEFRA SFNHFDRRKN GLMDHEDFRA CLISMGYDLG EAEFARIMTL
810 820 830 840 850
VDPNGQGTVT FQSFIDFMTR ETADTDTAEQ VIASFRILAS DKPYILAEEL
860 870 880 890
RRELPPDQAQ YCIKRMPPYS GPGSVPGALD YTAFSSALYG ESDL
Length:894
Mass (Da):103,834
Last modified:October 3, 2012 - v2
Checksum:i79FCDC9C85B3949C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti272V → G in AAF76325 (PubMed:11440986).Curated1
Sequence conflicti358P → A in AAF76325 (PubMed:11440986).Curated1
Sequence conflicti375W → C in AAF76325 (PubMed:11440986).Curated1
Sequence conflicti705A → G in AAF76325 (PubMed:11440986).Curated1
Sequence conflicti732A → G in AAF76325 (PubMed:11440986).Curated1
Sequence conflicti753E → K in AAF76325 (PubMed:11440986).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248643 mRNA Translation: AAF76325.1
AC154523 Genomic DNA No translation available.
CH466588 Genomic DNA Translation: EDL32304.1
CCDSiCCDS26239.1
RefSeqiNP_150371.4, NM_033268.4
UniGeneiMm.37638

Genome annotation databases

EnsembliENSMUST00000064204; ENSMUSP00000067708; ENSMUSG00000052374
ENSMUST00000168193; ENSMUSP00000129609; ENSMUSG00000052374
GeneIDi11472
KEGGimmu:11472
UCSCiuc007pli.1 mouse

Similar proteinsi

Entry informationi

Entry nameiACTN2_MOUSE
AccessioniPrimary (citable) accession number: Q9JI91
Secondary accession number(s): G3UW84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 3, 2012
Last modified: May 23, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health