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Q9JI91

- ACTN2_MOUSE

UniProt

Q9JI91 - ACTN2_MOUSE

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Protein

Alpha-actinin-2

Gene

Actn2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi766 – 777121; possibly ancestralAdd
BLAST
Calcium bindingi802 – 813122; possibly ancestralAdd
BLAST

GO - Molecular functioni

  1. actin filament binding Source: MGI
  2. calcium ion binding Source: InterPro
  3. cytoskeletal protein binding Source: UniProtKB
  4. ligand-dependent nuclear receptor transcription coactivator activity Source: MGI
  5. LIM domain binding Source: MGI
  6. protein binding, bridging Source: MGI
  7. protein homodimerization activity Source: UniProtKB
  8. thyroid hormone receptor coactivator activity Source: MGI

GO - Biological processi

  1. focal adhesion assembly Source: Ensembl
  2. microspike assembly Source: Ensembl
  3. muscle contraction Source: MGI
  4. negative regulation of potassium ion transmembrane transporter activity Source: Ensembl
  5. negative regulation of potassium ion transport Source: Ensembl
  6. negative regulation of protein localization to cell surface Source: Ensembl
  7. positive regulation of potassium ion transmembrane transporter activity Source: Ensembl
  8. positive regulation of potassium ion transport Source: Ensembl
  9. positive regulation of receptor activity Source: GOC
  10. protein homotetramerization Source: Ensembl
  11. regulation of membrane potential Source: Ensembl
  12. regulation of RNA biosynthetic process Source: GOC
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
REACT_198248. CREB phosphorylation through the activation of CaMKII.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-actinin-2
Alternative name(s):
Alpha-actinin skeletal muscle isoform 2
F-actin cross-linking protein
Gene namesi
Name:Actn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:109192. Actn2.

Subcellular locationi

CytoplasmmyofibrilsarcomereZ line 1 Publication
Note: Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. filopodium Source: Ensembl
  3. focal adhesion Source: Ensembl
  4. sarcomere Source: MGI
  5. striated muscle thin filament Source: MGI
  6. Z disc Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 894894Alpha-actinin-2PRO_0000073436Add
BLAST

Post-translational modificationi

Ubiquitinated by FBXL22, leading to proteasomal degradation.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ9JI91.
PaxDbiQ9JI91.
PRIDEiQ9JI91.

PTM databases

PhosphoSiteiQ9JI91.

Expressioni

Gene expression databases

CleanExiMM_ACTN2.
ExpressionAtlasiQ9JI91. baseline and differential.
GenevestigatoriQ9JI91.

Interactioni

Subunit structurei

Homodimer; antiparallel. Also forms heterodimers with ACTN3. Interacts with ADAM12, MYOZ1, MYOZ2 and MYOZ3. Interacts via its C-terminal region with the LDB3 PDZ domain. Interacts with XIRP2. Interacts with DST (via N-terminus). Interacts with PARVB (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Camk2aP117983EBI-299169,EBI-400384
Grin2bQ010974EBI-299169,EBI-400125

Protein-protein interaction databases

BioGridi197950. 7 interactions.
IntActiQ9JI91. 10 interactions.
MINTiMINT-97958.

Structurei

3D structure databases

ProteinModelPortaliQ9JI91.
SMRiQ9JI91. Positions 35-261, 274-894.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 254254Actin-bindingAdd
BLAST
Domaini38 – 142105CH 1PROSITE-ProRule annotationAdd
BLAST
Domaini151 – 254104CH 2PROSITE-ProRule annotationAdd
BLAST
Repeati281 – 391111Spectrin 1Add
BLAST
Repeati401 – 506106Spectrin 2Add
BLAST
Repeati516 – 627112Spectrin 3Add
BLAST
Repeati637 – 740104Spectrin 4Add
BLAST
Domaini753 – 78836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini789 – 82436EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the alpha-actinin family.Curated
Contains 1 actin-binding domain.Curated
Contains 2 CH (calponin-homology) domains.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 4 spectrin repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5069.
GeneTreeiENSGT00760000118813.
HOGENOMiHOG000263418.
HOVERGENiHBG050453.
InParanoidiQ9JI91.
KOiK05699.
OMAiRAKWDKV.
OrthoDBiEOG72C4ZJ.
TreeFamiTF352676.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
1.10.418.10. 2 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PfamiPF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
[Graphical view]
SMARTiSM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 3 hits.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JI91-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNQIEPGVQY NYVYDEDEYM IQEEEWDRDL LLDPAWEKQQ RKTFTAWCNS
60 70 80 90 100
HLRKAGTQIE NIEEDFRNGL KLMLLLEVIS GERLPKPDRG KMRFHKIANV
110 120 130 140 150
NKALDYIASK GVKLVSIGAE EIVDGNVKMT LGMIWTIILR FAIQDISVEE
160 170 180 190 200
TSAKEGLLLW CQRKTAPYRN VNIQNFHTSW KDGLGLCALI HRHRPDLIDY
210 220 230 240 250
SKLNKDDPIG NINLAMEIAE KHLDIPKMLD AEDIVNTPKP DERAIMTYVS
260 270 280 290 300
CFYHAFAGAE QAETAANRIC KVLAVNQENE RLMEEYERLA SELLEWIRRT
310 320 330 340 350
IPWLENRTPE KTMQAMQKKL EDFRDYRRKH KPPKVQEKCQ LEINFNTLQT
360 370 380 390 400
KLRISNRPAF MPSEGKMVSD IAGAWQRLEQ AEKGYEEWLL NEIRRLERLE
410 420 430 440 450
HLAEKFRQKA STHETWAYGK EQILLQKDYE SASLTEVRAL LRKHEAFESD
460 470 480 490 500
LAAHQDRVEQ IAAIAQELNE LDYHDAVNVN DRCQKICDQW DRLGTLTQKR
510 520 530 540 550
REALERTEKL LETIDQLHLE FAKRAAPFNN WMEGAMEDLQ DMFIVHSIEE
560 570 580 590 600
IQSLITAHEQ FKATLPEADG ERQSILAIQN EVEKVIQSYS IRISSSNPYS
610 620 630 640 650
TVTMDELRNK WDKVKQLVPV RDQSLQEELA RQHANERLRR QFAAQANAIG
660 670 680 690 700
PWIQNKMEEI ARSSIQITGA LEDQMNQLKQ YEHNIINYKN NIDKLEGDHQ
710 720 730 740 750
LIQEALVFDN KHTNYTMEHI RVGWELLLTT IARTINEVET QILTRDAKGI
760 770 780 790 800
TQEQMNEFRA SFNHFDRRKN GLMDHEDFRA CLISMGYDLG EAEFARIMTL
810 820 830 840 850
VDPNGQGTVT FQSFIDFMTR ETADTDTAEQ VIASFRILAS DKPYILAEEL
860 870 880 890
RRELPPDQAQ YCIKRMPPYS GPGSVPGALD YTAFSSALYG ESDL
Length:894
Mass (Da):103,834
Last modified:October 3, 2012 - v2
Checksum:i79FCDC9C85B3949C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti272 – 2721V → G in AAF76325. (PubMed:11440986)Curated
Sequence conflicti358 – 3581P → A in AAF76325. (PubMed:11440986)Curated
Sequence conflicti375 – 3751W → C in AAF76325. (PubMed:11440986)Curated
Sequence conflicti705 – 7051A → G in AAF76325. (PubMed:11440986)Curated
Sequence conflicti732 – 7321A → G in AAF76325. (PubMed:11440986)Curated
Sequence conflicti753 – 7531E → K in AAF76325. (PubMed:11440986)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF248643 mRNA. Translation: AAF76325.1.
AC154523 Genomic DNA. No translation available.
CH466588 Genomic DNA. Translation: EDL32304.1.
CCDSiCCDS26239.1.
RefSeqiNP_150371.4. NM_033268.4.
UniGeneiMm.37638.

Genome annotation databases

EnsembliENSMUST00000064204; ENSMUSP00000067708; ENSMUSG00000052374.
ENSMUST00000168193; ENSMUSP00000129609; ENSMUSG00000052374.
GeneIDi11472.
KEGGimmu:11472.
UCSCiuc007pli.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF248643 mRNA. Translation: AAF76325.1 .
AC154523 Genomic DNA. No translation available.
CH466588 Genomic DNA. Translation: EDL32304.1 .
CCDSi CCDS26239.1.
RefSeqi NP_150371.4. NM_033268.4.
UniGenei Mm.37638.

3D structure databases

ProteinModelPortali Q9JI91.
SMRi Q9JI91. Positions 35-261, 274-894.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 197950. 7 interactions.
IntActi Q9JI91. 10 interactions.
MINTi MINT-97958.

PTM databases

PhosphoSitei Q9JI91.

Proteomic databases

MaxQBi Q9JI91.
PaxDbi Q9JI91.
PRIDEi Q9JI91.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000064204 ; ENSMUSP00000067708 ; ENSMUSG00000052374 .
ENSMUST00000168193 ; ENSMUSP00000129609 ; ENSMUSG00000052374 .
GeneIDi 11472.
KEGGi mmu:11472.
UCSCi uc007pli.1. mouse.

Organism-specific databases

CTDi 88.
MGIi MGI:109192. Actn2.

Phylogenomic databases

eggNOGi COG5069.
GeneTreei ENSGT00760000118813.
HOGENOMi HOG000263418.
HOVERGENi HBG050453.
InParanoidi Q9JI91.
KOi K05699.
OMAi RAKWDKV.
OrthoDBi EOG72C4ZJ.
TreeFami TF352676.

Enzyme and pathway databases

Reactomei REACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
REACT_198248. CREB phosphorylation through the activation of CaMKII.

Miscellaneous databases

ChiTaRSi ACTN2. mouse.
NextBioi 278808.
PROi Q9JI91.
SOURCEi Search...

Gene expression databases

CleanExi MM_ACTN2.
ExpressionAtlasi Q9JI91. baseline and differential.
Genevestigatori Q9JI91.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
1.10.418.10. 2 hits.
InterProi IPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view ]
Pfami PF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
[Graphical view ]
SMARTi SM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 3 hits.
[Graphical view ]
SUPFAMi SSF47576. SSF47576. 1 hit.
PROSITEi PS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Differential expression of the actin-binding proteins, alpha-actinin-2 and -3, in different species: implications for the evolution of functional redundancy."
    Mills M., Yang N., Weinberger R., Vander Woude D.L., Beggs A.H., Easteal S., North K.N.
    Hum. Mol. Genet. 10:1335-1346(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha-actinin-2, is required for myoblast fusion."
    Galliano M.-F., Huet C., Frygelius J., Polgren A., Wewer U.M., Engvall E.
    J. Biol. Chem. 275:13933-13939(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ADAM12.
  5. "BPAG1 isoform-b: complex distribution pattern in striated and heart muscle and association with plectin and alpha-actinin."
    Steiner-Champliaud M.F., Schneider Y., Favre B., Paulhe F., Praetzel-Wunder S., Faulkner G., Konieczny P., Raith M., Wiche G., Adebola A., Liem R.K., Langbein L., Sonnenberg A., Fontao L., Borradori L.
    Exp. Cell Res. 316:297-313(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiACTN2_MOUSE
AccessioniPrimary (citable) accession number: Q9JI91
Secondary accession number(s): G3UW84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 3, 2012
Last modified: October 29, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3