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Protein

E3 ubiquitin-protein ligase RNF14

Gene

Rnf14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription (By similarity).By similarity

Caution

Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.Curated

Catalytic activityi

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei418By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri221 – 271RING-type 1; atypicalPROSITE-ProRule annotationAdd BLAST51
Zinc fingeri290 – 351IBR-typeAdd BLAST62
Zinc fingeri405 – 434RING-type 2; degeneratePROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF14 (EC:2.3.2.31By similarity)
Alternative name(s):
Androgen receptor-associated protein 54
Protein Triad2
RING finger protein 14
Gene namesi
Name:Rnf14
Synonyms:Ara54, Triad2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1929668 Rnf14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560581 – 485E3 ubiquitin-protein ligase RNF14Add BLAST485

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei349PhosphoserineBy similarity1

Post-translational modificationi

RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9JI90
PaxDbiQ9JI90
PeptideAtlasiQ9JI90
PRIDEiQ9JI90

PTM databases

iPTMnetiQ9JI90
PhosphoSitePlusiQ9JI90

Expressioni

Gene expression databases

BgeeiENSMUSG00000060450
CleanExiMM_RNF14
ExpressionAtlasiQ9JI90 baseline and differential
GenevisibleiQ9JI90 MM

Interactioni

Subunit structurei

Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2. Interacts with AR/androgen receptor; testosterone- and RNF6-regulated it promotes AR transcriptional activity.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi208150, 9 interactors
IntActiQ9JI90, 1 interactor
STRINGi10090.ENSMUSP00000072212

Structurei

3D structure databases

ProteinModelPortaliQ9JI90
SMRiQ9JI90
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 137RWDPROSITE-ProRule annotationAdd BLAST127

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45D-box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi173 – 176Poly-Ala4
Compositional biasi470 – 485Asp/Glu-rich (acidic)Add BLAST16

Domaini

The N-terminal destruction box (D-box) could act as a recognition signal for degradation via the ubiquitin-proteasome pathway.By similarity
The RING-type zinc finger is essential for the interaction with UBE2E2.By similarity
Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity

Sequence similaritiesi

Belongs to the RBR family. RNF14 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri221 – 271RING-type 1; atypicalPROSITE-ProRule annotationAdd BLAST51
Zinc fingeri290 – 351IBR-typeAdd BLAST62
Zinc fingeri405 – 434RING-type 2; degeneratePROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1814 Eukaryota
ENOG410Y5R1 LUCA
GeneTreeiENSGT00840000129738
HOGENOMiHOG000237334
HOVERGENiHBG057296
InParanoidiQ9JI90
KOiK11971
OMAiGDIWEDE
OrthoDBiEOG091G08QR
TreeFamiTF314401

Family and domain databases

Gene3Di3.10.110.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031127 E3_UB_ligase_RBR
IPR002867 IBR_dom
IPR006575 RWD-domain
IPR016135 UBQ-conjugating_enzyme/RWD
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR11685 PTHR11685, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 2 hits
PF05773 RWD, 1 hit
SMARTiView protein in SMART
SM00647 IBR, 2 hits
SM00591 RWD, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50908 RWD, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JI90-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAEDLEAQE DELLALASIY DADEFRKAES VQGGETRIYL DLPQNFKIFV
60 70 80 90 100
SGNSNESLQN SGFEYTICFL PPLVLNFELP PDYPSSSPPS FTLSGKWLSP
110 120 130 140 150
TQLSALCKHL DNLWEEHRGR VVLFAWMQFL KEETLTYLNI VSPFELKMGS
160 170 180 190 200
QKKVQRRATA QASSSTELGV GGAAAADVDQ EETVDERAVQ DVESLSSLIQ
210 220 230 240 250
EILDFNQARQ TKCFNSKLFL CSICFCEKLG SDCMYFLECK HVYCKACLKD
260 270 280 290 300
YFEIQIKDGQ VKCLNCPEPQ CPSVATPGQV KELVEADLFA RYDRLLLQST
310 320 330 340 350
LDLMADVVYC PRPCCQLPVM QEPGGTMAIC SSCNFAFCTL CRLTYHGLSP
360 370 380 390 400
CKVTAEKLID LRNEYLQADE ATKRFLEQRY GKRVIQKALE EMESKDWLEK
410 420 430 440 450
NSKSCPCCGT PIQKLDGCNK MTCTGCMQYF CWICMGSLSR ANPYRHFTDS
460 470 480
ESPCFNRLFH AVDINGDMWE DEIEEDDDDE DDDDD
Length:485
Mass (Da):54,926
Last modified:July 27, 2011 - v2
Checksum:i6228A9827F16FA47
GO
Isoform 2 (identifier: Q9JI90-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     52-79: GNSNESLQNSGFEYTICFLPPLVLNFEL → ALCSLQAPRQPVGRTPRQSGAVCLDAVS
     80-485: Missing.

Show »
Length:79
Mass (Da):8,631
Checksum:i5F0A682CF77C0EB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22A → V in AAF74266 (Ref. 1) Curated1
Sequence conflicti22A → V in AAF74267 (Ref. 1) Curated1
Sequence conflicti62G → R in BAB26503 (PubMed:16141072).Curated1
Sequence conflicti103 – 106LSAL → GTLV in BAB26503 (PubMed:16141072).Curated4
Sequence conflicti103 – 106LSAL → GTLV in BAB26741 (PubMed:16141072).Curated4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00575152 – 79GNSNE…LNFEL → ALCSLQAPRQPVGRTPRQSG AVCLDAVS in isoform 2. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_00575280 – 485Missing in isoform 2. 1 PublicationAdd BLAST406

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF249667 mRNA Translation: AAF74266.1
AF249668 mRNA Translation: AAF74267.1
AK011316 mRNA Translation: BAB27541.1
AK009783 mRNA Translation: BAB26503.1
AK010162 mRNA Translation: BAB26741.1
CH466528 Genomic DNA Translation: EDL10081.1
CH466528 Genomic DNA Translation: EDL10082.1
CH466528 Genomic DNA Translation: EDL10083.1
CH466528 Genomic DNA Translation: EDL10084.1
CH466528 Genomic DNA Translation: EDL10087.1
BC054841 mRNA Translation: AAH54841.1
BC094250 mRNA Translation: AAH94250.1
CCDSiCCDS29200.1 [Q9JI90-1]
RefSeqiNP_001158093.1, NM_001164621.1 [Q9JI90-1]
NP_001158094.1, NM_001164622.1
NP_064396.2, NM_020012.2 [Q9JI90-1]
XP_006526184.1, XM_006526121.2 [Q9JI90-1]
XP_006526185.1, XM_006526122.2 [Q9JI90-1]
XP_006526186.1, XM_006526123.2 [Q9JI90-1]
XP_006526187.1, XM_006526124.2 [Q9JI90-1]
XP_006526188.1, XM_006526125.2 [Q9JI90-1]
XP_006526189.1, XM_006526126.1 [Q9JI90-1]
XP_006526190.1, XM_006526127.2 [Q9JI90-1]
XP_017173444.1, XM_017317955.1 [Q9JI90-1]
XP_017173445.1, XM_017317956.1 [Q9JI90-1]
XP_017173446.1, XM_017317957.1 [Q9JI90-1]
UniGeneiMm.228903

Genome annotation databases

EnsembliENSMUST00000072376; ENSMUSP00000072212; ENSMUSG00000060450 [Q9JI90-1]
ENSMUST00000171461; ENSMUSP00000126205; ENSMUSG00000060450 [Q9JI90-1]
GeneIDi56736
KEGGimmu:56736
UCSCiuc008ese.2 mouse [Q9JI90-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRNF14_MOUSE
AccessioniPrimary (citable) accession number: Q9JI90
Secondary accession number(s): Q7TPR0
, Q9D0L2, Q9D6N2, Q9D6Z8, Q9JI89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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