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Q9JI85

- NUCB2_RAT

UniProt

Q9JI85 - NUCB2_RAT

Protein

Nucleobindin-2

Gene

Nucb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Calcium-binding protein. May have a role in calcium homeostasis By similarity.By similarity
    Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi171 – 22353By similarityAdd
    BLAST
    Calcium bindingi254 – 265121Add
    BLAST
    Calcium bindingi306 – 317122Add
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. DNA binding Source: UniProtKB-KW
    3. tumor necrosis factor receptor binding Source: RGD

    Keywords - Ligandi

    Calcium, DNA-binding, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleobindin-2
    Alternative name(s):
    DNA-binding protein NEFA
    Prepronesfatin
    Cleaved into the following chain:
    Gene namesi
    Name:Nucb2
    Synonyms:Nefa
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620888. Nucb2.

    Subcellular locationi

    Golgi apparatus By similarity. Endoplasmic reticulum By similarity. Nucleus envelope By similarity. Membrane By similarity; Peripheral membrane protein By similarity. Cytoplasm By similarity. Secreted By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-SubCell
    3. Golgi medial cisterna Source: RGD
    4. membrane Source: UniProtKB-SubCell
    5. nuclear envelope Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 420396Nucleobindin-2PRO_0000004167Add
    BLAST
    Chaini25 – 10682Nesfatin-1PRO_0000419821Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei332 – 3321PhosphoserineBy similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Phosphoprotein

    Proteomic databases

    PaxDbiQ9JI85.
    PRIDEiQ9JI85.

    PTM databases

    PhosphoSiteiQ9JI85.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9JI85.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9JI85. 1 interaction.
    MINTiMINT-4998273.
    STRINGi10116.ENSRNOP00000027753.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JI85.
    SMRiQ9JI85. Positions 229-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini241 – 27636EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini293 – 32836EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni213 – 420208Binds to necdinBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi284 – 29310Asp/Glu-rich (acidic)

    Sequence similaritiesi

    Belongs to the nucleobindin family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG262100.
    HOGENOMiHOG000007216.
    HOVERGENiHBG052685.
    InParanoidiQ9JI85.
    PhylomeDBiQ9JI85.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028813. NUCB2.
    [Graphical view]
    PANTHERiPTHR19237:SF22. PTHR19237:SF22. 1 hit.
    PfamiPF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9JI85-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRWRTIQARY CFLLVPCVLT ALEAVPIDVD KTKVHNVEPV ESARIEPPDT    50
    GLYYDEYLKQ VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR 100
    TRLDELKRQE VGRLRMLIKA KLDALQDTGM NHHLLLKQFE HLNHQNPDTF 150
    ESKDLDMLIK AATADLEQYD RTRHEEFKKY EMMKEHERRE YLKTLSEEKR 200
    KEEEAKFAEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF 250
    KLHDVNNDGF LDEQELEALF TKELDKVYNP QNAEDDMIEM EEERLRMREH 300
    VMNEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEEELKEY 350
    ESIIAIQESE LKKKADELQK QKEELQRQHD HLEAQKQEYQ QAVQQLEQKK 400
    FQQGIAPSGP AGELKFEPHT 420
    Length:420
    Mass (Da):50,090
    Last modified:October 1, 2000 - v1
    Checksum:iE7684E6C835F98FB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti38 – 381E → D in AAK66864. 1 PublicationCurated
    Sequence conflicti352 – 3521S → G in AAK66864. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF250142 mRNA. Translation: AAF75993.1.
    AF238223 mRNA. Translation: AAK66864.1.
    BC061778 mRNA. Translation: AAH61778.1.
    RefSeqiNP_067695.1. NM_021663.2.
    UniGeneiRn.41602.

    Genome annotation databases

    GeneIDi59295.
    KEGGirno:59295.
    UCSCiRGD:620888. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF250142 mRNA. Translation: AAF75993.1 .
    AF238223 mRNA. Translation: AAK66864.1 .
    BC061778 mRNA. Translation: AAH61778.1 .
    RefSeqi NP_067695.1. NM_021663.2.
    UniGenei Rn.41602.

    3D structure databases

    ProteinModelPortali Q9JI85.
    SMRi Q9JI85. Positions 229-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9JI85. 1 interaction.
    MINTi MINT-4998273.
    STRINGi 10116.ENSRNOP00000027753.

    PTM databases

    PhosphoSitei Q9JI85.

    Proteomic databases

    PaxDbi Q9JI85.
    PRIDEi Q9JI85.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 59295.
    KEGGi rno:59295.
    UCSCi RGD:620888. rat.

    Organism-specific databases

    CTDi 4925.
    RGDi 620888. Nucb2.

    Phylogenomic databases

    eggNOGi NOG262100.
    HOGENOMi HOG000007216.
    HOVERGENi HBG052685.
    InParanoidi Q9JI85.
    PhylomeDBi Q9JI85.

    Miscellaneous databases

    NextBioi 611821.
    PROi Q9JI85.

    Gene expression databases

    Genevestigatori Q9JI85.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028813. NUCB2.
    [Graphical view ]
    PANTHERi PTHR19237:SF22. PTHR19237:SF22. 1 hit.
    Pfami PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat NEFA mRNA."
      Hirschfeld G., Heiden I., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N.
      Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Ovary.
    2. "Characterization of rat NEFA protein."
      Hauge X., Neve K.A.
      Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    4. Cited for: FUNCTION OF NESFATIN.
    5. "A novel adipocytokine, nesfatin-1 modulates peripheral arterial contractility and blood pressure in rats."
      Yamawaki H., Takahashi M., Mukohda M., Morita T., Okada M., Hara Y.
      Biochem. Biophys. Res. Commun. 418:676-681(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF NESFATIN.

    Entry informationi

    Entry nameiNUCB2_RAT
    AccessioniPrimary (citable) accession number: Q9JI85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    NEFA stands for N=DNA-binding; EF=EF-hand; A=Acidic region.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3