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Q9JI85

- NUCB2_RAT

UniProt

Q9JI85 - NUCB2_RAT

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Protein

Nucleobindin-2

Gene

Nucb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Calcium-binding protein. May have a role in calcium homeostasis (By similarity).By similarity
Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi171 – 22353By similarityAdd
BLAST
Calcium bindingi254 – 265121Add
BLAST
Calcium bindingi306 – 317122Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. DNA binding Source: UniProtKB-KW
  3. tumor necrosis factor receptor binding Source: RGD
Complete GO annotation...

Keywords - Ligandi

Calcium, DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleobindin-2
Alternative name(s):
DNA-binding protein NEFA
Prepronesfatin
Cleaved into the following chain:
Gene namesi
Name:Nucb2
Synonyms:Nefa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620888. Nucb2.

Subcellular locationi

Golgi apparatus By similarity. Endoplasmic reticulum By similarity. Nucleus envelope By similarity. Membrane By similarity; Peripheral membrane protein By similarity. Cytoplasm By similarity. Secreted By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. Golgi medial cisterna Source: RGD
  4. membrane Source: UniProtKB-KW
  5. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 420396Nucleobindin-2PRO_0000004167Add
BLAST
Chaini25 – 10682Nesfatin-1PRO_0000419821Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei332 – 3321PhosphoserineBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Phosphoprotein

Proteomic databases

PaxDbiQ9JI85.
PRIDEiQ9JI85.

PTM databases

PhosphoSiteiQ9JI85.

Expressioni

Gene expression databases

GenevestigatoriQ9JI85.

Interactioni

Protein-protein interaction databases

IntActiQ9JI85. 1 interaction.
MINTiMINT-4998273.
STRINGi10116.ENSRNOP00000027753.

Structurei

3D structure databases

ProteinModelPortaliQ9JI85.
SMRiQ9JI85. Positions 229-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini241 – 27636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini293 – 32836EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni213 – 420208Binds to necdinBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi284 – 29310Asp/Glu-rich (acidic)

Sequence similaritiesi

Belongs to the nucleobindin family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG262100.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiQ9JI85.
PhylomeDBiQ9JI85.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028813. NUCB2.
[Graphical view]
PANTHERiPTHR19237:SF22. PTHR19237:SF22. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JI85-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRWRTIQARY CFLLVPCVLT ALEAVPIDVD KTKVHNVEPV ESARIEPPDT
60 70 80 90 100
GLYYDEYLKQ VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR
110 120 130 140 150
TRLDELKRQE VGRLRMLIKA KLDALQDTGM NHHLLLKQFE HLNHQNPDTF
160 170 180 190 200
ESKDLDMLIK AATADLEQYD RTRHEEFKKY EMMKEHERRE YLKTLSEEKR
210 220 230 240 250
KEEEAKFAEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF
260 270 280 290 300
KLHDVNNDGF LDEQELEALF TKELDKVYNP QNAEDDMIEM EEERLRMREH
310 320 330 340 350
VMNEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEEELKEY
360 370 380 390 400
ESIIAIQESE LKKKADELQK QKEELQRQHD HLEAQKQEYQ QAVQQLEQKK
410 420
FQQGIAPSGP AGELKFEPHT
Length:420
Mass (Da):50,090
Last modified:October 1, 2000 - v1
Checksum:iE7684E6C835F98FB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381E → D in AAK66864. 1 PublicationCurated
Sequence conflicti352 – 3521S → G in AAK66864. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF250142 mRNA. Translation: AAF75993.1.
AF238223 mRNA. Translation: AAK66864.1.
BC061778 mRNA. Translation: AAH61778.1.
RefSeqiNP_067695.1. NM_021663.2.
UniGeneiRn.41602.

Genome annotation databases

GeneIDi59295.
KEGGirno:59295.
UCSCiRGD:620888. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF250142 mRNA. Translation: AAF75993.1 .
AF238223 mRNA. Translation: AAK66864.1 .
BC061778 mRNA. Translation: AAH61778.1 .
RefSeqi NP_067695.1. NM_021663.2.
UniGenei Rn.41602.

3D structure databases

ProteinModelPortali Q9JI85.
SMRi Q9JI85. Positions 229-327.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9JI85. 1 interaction.
MINTi MINT-4998273.
STRINGi 10116.ENSRNOP00000027753.

PTM databases

PhosphoSitei Q9JI85.

Proteomic databases

PaxDbi Q9JI85.
PRIDEi Q9JI85.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 59295.
KEGGi rno:59295.
UCSCi RGD:620888. rat.

Organism-specific databases

CTDi 4925.
RGDi 620888. Nucb2.

Phylogenomic databases

eggNOGi NOG262100.
HOGENOMi HOG000007216.
HOVERGENi HBG052685.
InParanoidi Q9JI85.
PhylomeDBi Q9JI85.

Miscellaneous databases

NextBioi 611821.
PROi Q9JI85.

Gene expression databases

Genevestigatori Q9JI85.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028813. NUCB2.
[Graphical view ]
PANTHERi PTHR19237:SF22. PTHR19237:SF22. 1 hit.
Pfami PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 2 hits.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rat NEFA mRNA."
    Hirschfeld G., Heiden I., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Ovary.
  2. "Characterization of rat NEFA protein."
    Hauge X., Neve K.A.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  4. Cited for: FUNCTION OF NESFATIN.
  5. "A novel adipocytokine, nesfatin-1 modulates peripheral arterial contractility and blood pressure in rats."
    Yamawaki H., Takahashi M., Mukohda M., Morita T., Okada M., Hara Y.
    Biochem. Biophys. Res. Commun. 418:676-681(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF NESFATIN.

Entry informationi

Entry nameiNUCB2_RAT
AccessioniPrimary (citable) accession number: Q9JI85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

NEFA stands for N=DNA-binding; EF=EF-hand; A=Acidic region.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3