Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9JI85 (NUCB2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleobindin-2
Alternative name(s):
DNA-binding protein NEFA
Prepronesfatin

Cleaved into the following chain:

  1. Nesfatin-1
Gene names
Name:Nucb2
Synonyms:Nefa
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium-binding protein. May have a role in calcium homeostasis By similarity. Ref.4 Ref.5

Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance. Ref.4 Ref.5

Subcellular location

Golgi apparatus By similarity. Endoplasmic reticulum By similarity. Nucleus envelope By similarity. Membrane; Peripheral membrane protein By similarity. Cytoplasm By similarity. Secreted By similarity.

Nesfatin-1: Secreted.

Miscellaneous

NEFA stands for N=DNA-binding; EF=EF-hand; A=Acidic region.

Sequence similarities

Belongs to the nucleobindin family.

Contains 2 EF-hand domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 420396Nucleobindin-2
PRO_0000004167
Chain25 – 10682Nesfatin-1
PRO_0000419821

Regions

Domain241 – 27636EF-hand 1
Domain293 – 32836EF-hand 2
DNA binding171 – 22353 By similarity
Calcium binding254 – 265121
Calcium binding306 – 317122
Region213 – 420208Binds to necdin By similarity
Compositional bias284 – 29310Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue3321Phosphoserine By similarity

Experimental info

Sequence conflict381E → D in AAK66864. Ref.2
Sequence conflict3521S → G in AAK66864. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9JI85 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: E7684E6C835F98FB

FASTA42050,090
        10         20         30         40         50         60 
MRWRTIQARY CFLLVPCVLT ALEAVPIDVD KTKVHNVEPV ESARIEPPDT GLYYDEYLKQ 

        70         80         90        100        110        120 
VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR TRLDELKRQE VGRLRMLIKA 

       130        140        150        160        170        180 
KLDALQDTGM NHHLLLKQFE HLNHQNPDTF ESKDLDMLIK AATADLEQYD RTRHEEFKKY 

       190        200        210        220        230        240 
EMMKEHERRE YLKTLSEEKR KEEEAKFAEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD 

       250        260        270        280        290        300 
PNDFDPKTFF KLHDVNNDGF LDEQELEALF TKELDKVYNP QNAEDDMIEM EEERLRMREH 

       310        320        330        340        350        360 
VMNEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEEELKEY ESIIAIQESE 

       370        380        390        400        410        420 
LKKKADELQK QKEELQRQHD HLEAQKQEYQ QAVQQLEQKK FQQGIAPSGP AGELKFEPHT 

« Hide

References

« Hide 'large scale' references
[1]"Rat NEFA mRNA."
Hirschfeld G., Heiden I., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Ovary.
[2]"Characterization of rat NEFA protein."
Hauge X., Neve K.A.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[4]"Identification of nesfatin-1 as a satiety molecule in the hypothalamus."
Oh-I S., Shimizu H., Satoh T., Okada S., Adachi S., Inoue K., Eguchi H., Yamamoto M., Imaki T., Hashimoto K., Tsuchiya T., Monden T., Horiguchi K., Yamada M., Mori M.
Nature 443:709-712(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF NESFATIN.
[5]"A novel adipocytokine, nesfatin-1 modulates peripheral arterial contractility and blood pressure in rats."
Yamawaki H., Takahashi M., Mukohda M., Morita T., Okada M., Hara Y.
Biochem. Biophys. Res. Commun. 418:676-681(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF NESFATIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF250142 mRNA. Translation: AAF75993.1.
AF238223 mRNA. Translation: AAK66864.1.
BC061778 mRNA. Translation: AAH61778.1.
RefSeqNP_067695.1. NM_021663.2.
UniGeneRn.41602.

3D structure databases

ProteinModelPortalQ9JI85.
SMRQ9JI85. Positions 229-327.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9JI85. 1 interaction.
MINTMINT-4998273.
STRING10116.ENSRNOP00000027753.

PTM databases

PhosphoSiteQ9JI85.

Proteomic databases

PaxDbQ9JI85.
PRIDEQ9JI85.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID59295.
KEGGrno:59295.
UCSCRGD:620888. rat.

Organism-specific databases

CTD4925.
RGD620888. Nucb2.

Phylogenomic databases

eggNOGNOG262100.
HOGENOMHOG000007216.
HOVERGENHBG052685.
InParanoidQ9JI85.
PhylomeDBQ9JI85.

Gene expression databases

GenevestigatorQ9JI85.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028813. NUCB2.
[Graphical view]
PANTHERPTHR19237:SF3. PTHR19237:SF3. 1 hit.
PfamPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 2 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio611821.
PROQ9JI85.

Entry information

Entry nameNUCB2_RAT
AccessionPrimary (citable) accession number: Q9JI85
Entry history
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families