Reviewed, UniProtKB/Swiss-Prot Q9JI85 (NUCB2_RAT)

Names and origin

Protein names

Recommended name:
    Nucleobindin-2
Alternative name(s):
    DNA-binding protein NEFA

Gene names

Name:	Nucb2
Synonyms:	Nefa

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Calcium-binding protein. May have a role in calcium homeostasis By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity. Cytoplasm By similarity. Secreted By similarity.
Miscellaneous	NEFA stands for N=DNA-binding; EF=EF-hand; A=Acidic region.
Sequence similarities	Belongs to the nucleobindin family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Secreted
Domain	Repeat Signal
Ligand	Calcium DNA-binding
Gene Ontology (GO)
Cellular component	Golgi medial cisterna Inferred from direct assay. Source: RGD extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 420	396	Nucleobindin-2		PRO_0000004167
Regions
	Domain	241 – 276	36	EF-hand 1
	Domain	293 – 328	36	EF-hand 2
	DNA binding	171 – 223	53	By similarity
	Calcium binding	254 – 265	12	1
	Calcium binding	306 – 317	12	2
	Region	213 – 420	208	Binds to necdin By similarity
	Compositional bias	284 – 293	10	Asp/Glu-rich (acidic)
Experimental info
	Sequence conflict	38	1	E → D in AAK66864. Ref.2
	Sequence conflict	352	1	S → G in AAK66864. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9JI85-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E7684E6C835F98FB	FASTA	420	50,090
	10 20 30 40 50 60 MRWRTIQARY CFLLVPCVLT ALEAVPIDVD KTKVHNVEPV ESARIEPPDT GLYYDEYLKQ 70 80 90 100 110 120 VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR TRLDELKRQE VGRLRMLIKA 130 140 150 160 170 180 KLDALQDTGM NHHLLLKQFE HLNHQNPDTF ESKDLDMLIK AATADLEQYD RTRHEEFKKY 190 200 210 220 230 240 EMMKEHERRE YLKTLSEEKR KEEEAKFAEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD 250 260 270 280 290 300 PNDFDPKTFF KLHDVNNDGF LDEQELEALF TKELDKVYNP QNAEDDMIEM EEERLRMREH 310 320 330 340 350 360 VMNEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEEELKEY ESIIAIQESE 370 380 390 400 410 420 LKKKADELQK QKEELQRQHD HLEAQKQEYQ QAVQQLEQKK FQQGIAPSGP AGELKFEPHT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat NEFA mRNA." Hirschfeld G., Heiden I., Barnikol-Watanabe S., Barnikol H.U., Hilschmann N. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Ovary.
[2]	"Characterization of rat NEFA protein." Hauge X., Neve K.A. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF250142 mRNA. Translation: AAF75993.1. AF238223 mRNA. Translation: AAK66864.1. BC061778 mRNA. Translation: AAH61778.1.
IPI	IPI00200070.
RefSeq	NP_067695.1.
UniGene	Rn.41602
3D structure databases
SMR	Q9JI85. Positions 229-327.
ModBase	Search...
PTM databases
PhosphoSite	Q9JI85.
Genome annotation databases
Ensembl	ENSRNOT00000027752; ENSRNOP00000027753; ENSRNOG00000020456; Rattus norvegicus. [Genome view]
GeneID	59295.
KEGG	rno:59295.
UCSC	NM_021663. rat.
Organism-specific databases
CTD	59295.
RGD	620888. Nucb2.
Phylogenomic databases
eggNOG	roNOG09966.
HOVERGEN	Q9JI85.
InParanoid	Q9JI85.
Gene expression databases
ArrayExpress	Q9JI85.
Genevestigator	Q9JI85.
GermOnline	ENSRNOG00000020456. Rattus norvegicus.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	611821.

Entry information

Entry name

NUCB2_RAT

Accession

Primary (citable) accession number: Q9JI85

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)