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Protein

Disintegrin and metalloproteinase domain-containing protein 21

Gene

Adam21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in sperm maturation and/or fertilization. May also be involved in epithelia functions associated with establishing and maintaining gradients of ions or nutrients.

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi178Zinc; in inhibited formBy similarity1
Metal bindingi345Zinc; catalyticSequence analysis1
Active sitei346PROSITE-ProRule annotation1
Metal bindingi349Zinc; catalyticSequence analysis1
Metal bindingi355Zinc; catalyticSequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1300644. Interaction With The Zona Pellucida.

Protein family/group databases

MEROPSiM12.233.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 21 (EC:3.4.24.-)
Short name:
ADAM 21
Alternative name(s):
Disintegrin and metalloproteinase domain-containing protein 31
Short name:
ADAM 31
Gene namesi
Name:Adam21
Synonyms:Adam31
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1861229. Adam21.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini210 – 685ExtracellularSequence analysisAdd BLAST476
Transmembranei686 – 706HelicalSequence analysisAdd BLAST21
Topological domaini707 – 729CytoplasmicSequence analysisAdd BLAST23

GO - Cellular componenti

  • axon Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • neuronal cell body Source: MGI
  • neuron projection Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 39Sequence analysisAdd BLAST39
PropeptideiPRO_000002911040 – 209Sequence analysisAdd BLAST170
ChainiPRO_0000029111210 – 729Disintegrin and metalloproteinase domain-containing protein 21Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi169N-linked (GlcNAc...)Sequence analysis1
Glycosylationi231N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi320 ↔ 397By similarity
Disulfide bondi360 ↔ 382By similarity
Disulfide bondi362 ↔ 367By similarity
Glycosylationi381N-linked (GlcNAc...)Sequence analysis1
Glycosylationi441N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi468 ↔ 488By similarity
Glycosylationi482N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi638 ↔ 649By similarity
Disulfide bondi643 ↔ 655By similarity
Disulfide bondi657 ↔ 666By similarity

Post-translational modificationi

Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9JI76.
PRIDEiQ9JI76.

PTM databases

iPTMnetiQ9JI76.
PhosphoSitePlusiQ9JI76.

Expressioni

Tissue specificityi

Highly expressed in Leydig cells. Expressed also in cauda epididymidis, vas deferens, convoluted tubules, kidney and the parietal cells of stomach. Not detected on developing spermatocytes or mature sperm.

Gene expression databases

BgeeiENSMUSG00000008438.
CleanExiMM_ADAM21.
GenevisibleiQ9JI76. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000008582.

Structurei

3D structure databases

ProteinModelPortaliQ9JI76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini212 – 402Peptidase M12BPROSITE-ProRule annotationAdd BLAST191
Domaini410 – 496DisintegrinPROSITE-ProRule annotationAdd BLAST87
Domaini638 – 667EGF-likePROSITE-ProRule annotationAdd BLAST30

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi176 – 183Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi497 – 639Cys-richAdd BLAST143

Domaini

A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ9JI76.
KOiK08610.
OMAiHSATFEH.
OrthoDBiEOG091G03BZ.
PhylomeDBiQ9JI76.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JI76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MECFIMLGAD ARTLMRVTLL LLWLKALPSL IDLSQTGSTQ YLSSPEVVIP
60 70 80 90 100
LKVTSRARGA KNSEWLSYSL VFGGRRHVVH MRVKKLLVST HIPVLTYTEE
110 120 130 140 150
HTPLSDYPFV PSDCYYHGYV EGALESLVAF SACNGGLQGV LQMNGFSYEI
160 170 180 190 200
EPIKHSSTFE HLVYTLNNNK TQFPPMLCSL TEKRLLYQPF GVEEAKKSAM
210 220 230 240 250
KQNYGKLWPH MWFLELAVVV DYGFFTNAQQ NLSKVRGDVV LVVNMVDSMY
260 270 280 290 300
KPLDTYVTLV GIEIWNRGNV LPMENIHQVL EDFSHWKQIS LSQVHHDAAH
310 320 330 340 350
IFIRSSLISV LGIAYIAGIC RPPLDCGVEN FQGDAWSLFA NTVAHELGHT
360 370 380 390 400
FGMKHDEESC SCGKSGCVMS TFRVPAERFT NCSYSDFMKT TLNQGTCLYN
410 420 430 440 450
HPRPGAGFLV KRCGNGMVES EEECDCGSVQ ECEQDPCCFL NCTLRPAAAC
460 470 480 490 500
SFGLCCKDCK FMLLGELCRP KINECDLPEW CNGTSHQCPE DGYVQDGVPC
510 520 530 540 550
GAGAYCYQKQ CNNHDQQCRE IFGKGARSAS HNCYKEINLQ GNRFGHCGTD
560 570 580 590 600
GTVFLKCRMS DVFCGKVHCE NVEDIHHPQA PYVLQNIYAN GITCWSTGHC
610 620 630 640 650
LGMGVPDVGE VKDGTTCGVG KICLHKKCVS LSVLSNACLP ETCNRKGVCN
660 670 680 690 700
NKHHCHCDYG WSPPFCLHRG YGGSIDSGPT SQKRRVIITV LSITVPVLSI
710 720
LICLLIAGLY RIYCKIPSGP KETKASSPG
Length:729
Mass (Da):80,850
Last modified:October 1, 2000 - v1
Checksum:iE70B79BC46B64549
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF251559 mRNA. Translation: AAF74731.1.
AK014827 mRNA. Translation: BAB29569.1.
BC132152 mRNA. Translation: AAI32153.1.
BC132344 mRNA. Translation: AAI32345.1.
CCDSiCCDS26022.1.
RefSeqiNP_065063.1. NM_020330.5.
UniGeneiMm.117130.

Genome annotation databases

EnsembliENSMUST00000008582; ENSMUSP00000008582; ENSMUSG00000008438.
GeneIDi56622.
KEGGimmu:56622.
UCSCiuc007ock.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF251559 mRNA. Translation: AAF74731.1.
AK014827 mRNA. Translation: BAB29569.1.
BC132152 mRNA. Translation: AAI32153.1.
BC132344 mRNA. Translation: AAI32345.1.
CCDSiCCDS26022.1.
RefSeqiNP_065063.1. NM_020330.5.
UniGeneiMm.117130.

3D structure databases

ProteinModelPortaliQ9JI76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000008582.

Protein family/group databases

MEROPSiM12.233.

PTM databases

iPTMnetiQ9JI76.
PhosphoSitePlusiQ9JI76.

Proteomic databases

PaxDbiQ9JI76.
PRIDEiQ9JI76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008582; ENSMUSP00000008582; ENSMUSG00000008438.
GeneIDi56622.
KEGGimmu:56622.
UCSCiuc007ock.1. mouse.

Organism-specific databases

CTDi8747.
MGIiMGI:1861229. Adam21.

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ9JI76.
KOiK08610.
OMAiHSATFEH.
OrthoDBiEOG091G03BZ.
PhylomeDBiQ9JI76.
TreeFamiTF314733.

Enzyme and pathway databases

ReactomeiR-MMU-1300644. Interaction With The Zona Pellucida.

Miscellaneous databases

PROiQ9JI76.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008438.
CleanExiMM_ADAM21.
GenevisibleiQ9JI76. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADA21_MOUSE
AccessioniPrimary (citable) accession number: Q9JI76
Secondary accession number(s): A2RSL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.