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Protein

Disintegrin and metalloproteinase domain-containing protein 21

Gene

Adam21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in sperm maturation and/or fertilization. May also be involved in epithelia functions associated with establishing and maintaining gradients of ions or nutrients.

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi178 – 1781Zinc; in inhibited formBy similarity
Metal bindingi345 – 3451Zinc; catalyticSequence analysis
Active sitei346 – 3461PROSITE-ProRule annotation
Metal bindingi349 – 3491Zinc; catalyticSequence analysis
Metal bindingi355 – 3551Zinc; catalyticSequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1300644. Interaction With The Zona Pellucida.

Protein family/group databases

MEROPSiM12.233.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 21 (EC:3.4.24.-)
Short name:
ADAM 21
Alternative name(s):
Disintegrin and metalloproteinase domain-containing protein 31
Short name:
ADAM 31
Gene namesi
Name:Adam21
Synonyms:Adam31
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1861229. Adam21.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini210 – 685476ExtracellularSequence analysisAdd
BLAST
Transmembranei686 – 70621HelicalSequence analysisAdd
BLAST
Topological domaini707 – 72923CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • axon Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • neuronal cell body Source: MGI
  • neuron projection Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3939Sequence analysisAdd
BLAST
Propeptidei40 – 209170Sequence analysisPRO_0000029110Add
BLAST
Chaini210 – 729520Disintegrin and metalloproteinase domain-containing protein 21PRO_0000029111Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence analysis
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence analysis
Disulfide bondi320 ↔ 397By similarity
Disulfide bondi360 ↔ 382By similarity
Disulfide bondi362 ↔ 367By similarity
Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence analysis
Disulfide bondi468 ↔ 488By similarity
Glycosylationi482 – 4821N-linked (GlcNAc...)Sequence analysis
Disulfide bondi638 ↔ 649By similarity
Disulfide bondi643 ↔ 655By similarity
Disulfide bondi657 ↔ 666By similarity

Post-translational modificationi

Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9JI76.
PRIDEiQ9JI76.

PTM databases

iPTMnetiQ9JI76.
PhosphoSiteiQ9JI76.

Expressioni

Tissue specificityi

Highly expressed in Leydig cells. Expressed also in cauda epididymidis, vas deferens, convoluted tubules, kidney and the parietal cells of stomach. Not detected on developing spermatocytes or mature sperm.

Gene expression databases

BgeeiQ9JI76.
CleanExiMM_ADAM21.
GenevisibleiQ9JI76. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000008582.

Structurei

3D structure databases

ProteinModelPortaliQ9JI76.
SMRiQ9JI76. Positions 213-632.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini212 – 402191Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini410 – 49687DisintegrinPROSITE-ProRule annotationAdd
BLAST
Domaini638 – 66730EGF-likePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi176 – 1838Cysteine switchBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi497 – 639143Cys-richAdd
BLAST

Domaini

A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ9JI76.
KOiK08610.
OMAiHSATFEH.
OrthoDBiEOG7KWSGW.
PhylomeDBiQ9JI76.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JI76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MECFIMLGAD ARTLMRVTLL LLWLKALPSL IDLSQTGSTQ YLSSPEVVIP
60 70 80 90 100
LKVTSRARGA KNSEWLSYSL VFGGRRHVVH MRVKKLLVST HIPVLTYTEE
110 120 130 140 150
HTPLSDYPFV PSDCYYHGYV EGALESLVAF SACNGGLQGV LQMNGFSYEI
160 170 180 190 200
EPIKHSSTFE HLVYTLNNNK TQFPPMLCSL TEKRLLYQPF GVEEAKKSAM
210 220 230 240 250
KQNYGKLWPH MWFLELAVVV DYGFFTNAQQ NLSKVRGDVV LVVNMVDSMY
260 270 280 290 300
KPLDTYVTLV GIEIWNRGNV LPMENIHQVL EDFSHWKQIS LSQVHHDAAH
310 320 330 340 350
IFIRSSLISV LGIAYIAGIC RPPLDCGVEN FQGDAWSLFA NTVAHELGHT
360 370 380 390 400
FGMKHDEESC SCGKSGCVMS TFRVPAERFT NCSYSDFMKT TLNQGTCLYN
410 420 430 440 450
HPRPGAGFLV KRCGNGMVES EEECDCGSVQ ECEQDPCCFL NCTLRPAAAC
460 470 480 490 500
SFGLCCKDCK FMLLGELCRP KINECDLPEW CNGTSHQCPE DGYVQDGVPC
510 520 530 540 550
GAGAYCYQKQ CNNHDQQCRE IFGKGARSAS HNCYKEINLQ GNRFGHCGTD
560 570 580 590 600
GTVFLKCRMS DVFCGKVHCE NVEDIHHPQA PYVLQNIYAN GITCWSTGHC
610 620 630 640 650
LGMGVPDVGE VKDGTTCGVG KICLHKKCVS LSVLSNACLP ETCNRKGVCN
660 670 680 690 700
NKHHCHCDYG WSPPFCLHRG YGGSIDSGPT SQKRRVIITV LSITVPVLSI
710 720
LICLLIAGLY RIYCKIPSGP KETKASSPG
Length:729
Mass (Da):80,850
Last modified:October 1, 2000 - v1
Checksum:iE70B79BC46B64549
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF251559 mRNA. Translation: AAF74731.1.
AK014827 mRNA. Translation: BAB29569.1.
BC132152 mRNA. Translation: AAI32153.1.
BC132344 mRNA. Translation: AAI32345.1.
CCDSiCCDS26022.1.
RefSeqiNP_065063.1. NM_020330.5.
UniGeneiMm.117130.

Genome annotation databases

EnsembliENSMUST00000008582; ENSMUSP00000008582; ENSMUSG00000008438.
GeneIDi56622.
KEGGimmu:56622.
UCSCiuc007ock.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF251559 mRNA. Translation: AAF74731.1.
AK014827 mRNA. Translation: BAB29569.1.
BC132152 mRNA. Translation: AAI32153.1.
BC132344 mRNA. Translation: AAI32345.1.
CCDSiCCDS26022.1.
RefSeqiNP_065063.1. NM_020330.5.
UniGeneiMm.117130.

3D structure databases

ProteinModelPortaliQ9JI76.
SMRiQ9JI76. Positions 213-632.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000008582.

Protein family/group databases

MEROPSiM12.233.

PTM databases

iPTMnetiQ9JI76.
PhosphoSiteiQ9JI76.

Proteomic databases

PaxDbiQ9JI76.
PRIDEiQ9JI76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008582; ENSMUSP00000008582; ENSMUSG00000008438.
GeneIDi56622.
KEGGimmu:56622.
UCSCiuc007ock.1. mouse.

Organism-specific databases

CTDi8747.
MGIiMGI:1861229. Adam21.

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ9JI76.
KOiK08610.
OMAiHSATFEH.
OrthoDBiEOG7KWSGW.
PhylomeDBiQ9JI76.
TreeFamiTF314733.

Enzyme and pathway databases

ReactomeiR-MMU-1300644. Interaction With The Zona Pellucida.

Miscellaneous databases

PROiQ9JI76.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JI76.
CleanExiMM_ADAM21.
GenevisibleiQ9JI76. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of ADAM 31: a protein expressed in Leydig cells and specialized epithelia."
    Liu L., Smith J.W.
    Endocrinology 141:2033-2042(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Bone marrow.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiADA21_MOUSE
AccessioniPrimary (citable) accession number: Q9JI76
Secondary accession number(s): A2RSL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.