ID   B3GT5_MOUSE             Reviewed;         308 AA.
AC   Q9JI67;
DT   21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   24-JAN-2024, entry version 148.
DE   RecName: Full=Beta-1,3-galactosyltransferase 5 {ECO:0000305};
DE            Short=Beta-1,3-GalTase 5;
DE            Short=Beta3Gal-T5;
DE            Short=Beta3GalT5;
DE            Short=b3Gal-T5;
DE            EC=2.4.1.-;
DE   AltName: Full=Beta-3-Gx-T5;
DE   AltName: Full=Stage-specific embryonic antigen 3 synthase;
DE            Short=SSEA-3 synthase;
DE   AltName: Full=UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5;
DE   AltName: Full=UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5;
GN   Name=B3galt5 {ECO:0000312|MGI:MGI:2136878}; Synonyms=B3gt5;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/SvJ;
RX   PubMed=10837462; DOI=10.1074/jbc.c000263200;
RA   Zhou D., Henion T.R., Jungalwala F.B., Berger E.G., Hennet T.;
RT   "The beta1,3-galactosyltransferase beta3GalT-V is a stage-specific
RT   embryonic antigen-3 (SSEA-3) synthase.";
RL   J. Biol. Chem. 275:22631-22634(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Catalyzes the transfer of Gal to GlcNAc-based acceptors with
CC       a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc
CC       structure. Can use glycolipid LC3Cer as an efficient acceptor. Also
CC       catalyzes the transfer of Gal to the terminal GalNAc unit of the
CC       globoside GB4, thereby synthesizing the glycolipid GB5, also known as
CC       the stage-specific embryonic antigen-3 (SSEA-3).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a globoside Gb4Cer (d18:1(4E)) + UDP-alpha-D-galactose = a
CC         globoside GalGb4Cer (d18:1(4E)) + H(+) + UDP; Xref=Rhea:RHEA:41996,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18259, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:62571, ChEBI:CHEBI:66914;
CC         Evidence={ECO:0000250|UniProtKB:Q9Y2C3};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41997;
CC         Evidence={ECO:0000250|UniProtKB:Q9Y2C3};
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC       pass type II membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in brain and kidney.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 31 family.
CC       {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Functional Glycomics Gateway - GTase; Note=b3GalT5;
CC       URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_mou_458";
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DR   EMBL; AF254738; AAF86241.1; -; Genomic_DNA.
DR   EMBL; BC057887; AAH57887.1; -; mRNA.
DR   CCDS; CCDS28356.1; -.
DR   RefSeq; NP_001116465.1; NM_001122993.1.
DR   RefSeq; NP_149161.1; NM_033149.3.
DR   RefSeq; XP_006523179.1; XM_006523116.3.
DR   RefSeq; XP_006523180.1; XM_006523117.1.
DR   RefSeq; XP_017172660.1; XM_017317171.1.
DR   AlphaFoldDB; Q9JI67; -.
DR   SMR; Q9JI67; -.
DR   STRING; 10090.ENSMUSP00000109431; -.
DR   CAZy; GT31; Glycosyltransferase Family 31.
DR   GlyCosmos; Q9JI67; 3 sites, No reported glycans.
DR   GlyGen; Q9JI67; 3 sites.
DR   PhosphoSitePlus; Q9JI67; -.
DR   MaxQB; Q9JI67; -.
DR   PaxDb; 10090-ENSMUSP00000109431; -.
DR   ProteomicsDB; 277153; -.
DR   Antibodypedia; 23457; 111 antibodies from 24 providers.
DR   DNASU; 93961; -.
DR   Ensembl; ENSMUST00000099497.4; ENSMUSP00000097096.4; ENSMUSG00000074892.10.
DR   Ensembl; ENSMUST00000113800.9; ENSMUSP00000109431.3; ENSMUSG00000074892.10.
DR   GeneID; 93961; -.
DR   KEGG; mmu:93961; -.
DR   UCSC; uc008acv.2; mouse.
DR   AGR; MGI:2136878; -.
DR   CTD; 10317; -.
DR   MGI; MGI:2136878; B3galt5.
DR   VEuPathDB; HostDB:ENSMUSG00000074892; -.
DR   eggNOG; KOG2287; Eukaryota.
DR   GeneTree; ENSGT00940000160964; -.
DR   HOGENOM; CLU_036849_2_4_1; -.
DR   InParanoid; Q9JI67; -.
DR   OMA; RFNKWFV; -.
DR   OrthoDB; 532757at2759; -.
DR   PhylomeDB; Q9JI67; -.
DR   TreeFam; TF318639; -.
DR   Reactome; R-MMU-9037629; Lewis blood group biosynthesis.
DR   UniPathway; UPA00378; -.
DR   BioGRID-ORCS; 93961; 3 hits in 79 CRISPR screens.
DR   ChiTaRS; B3galt5; mouse.
DR   PRO; PR:Q9JI67; -.
DR   Proteomes; UP000000589; Chromosome 16.
DR   RNAct; Q9JI67; Protein.
DR   Bgee; ENSMUSG00000074892; Expressed in left colon and 117 other cell types or tissues.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0008499; F:UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006493; P:protein O-linked glycosylation; IBA:GO_Central.
DR   GO; GO:0009617; P:response to bacterium; IEP:MGI.
DR   Gene3D; 3.90.550.50; -; 1.
DR   InterPro; IPR002659; Glyco_trans_31.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR11214:SF265; BETA-1,3-GALACTOSYLTRANSFERASE 5; 1.
DR   PANTHER; PTHR11214; BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE; 1.
DR   Pfam; PF01762; Galactosyl_T; 1.
DR   SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
DR   Genevisible; Q9JI67; MM.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Glycosyltransferase; Golgi apparatus; Lipid metabolism;
KW   Membrane; Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..308
FT                   /note="Beta-1,3-galactosyltransferase 5"
FT                   /id="PRO_0000219165"
FT   TOPO_DOM        1..7
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        8..25
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..308
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        128
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        172
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        229
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   308 AA;  35964 MW;  789073A5178825B1 CRC64;
     MAHMKTRLVY ASILMMGALC LYFSMDSFRE LPFVFKKSHG KFLQIPDIDC KQKPPFLVLL
     VTSSHKQLAA RMAIRKTWGR ETSVQGQQVR TFFLLGTSDS TEEMDATTLE SEQHRDIIQK
     DFKDAYFNLT LKTMMGMEWV YHFCPQTAYV MKTDSDMFVN VGYLTELLLK KNKTTRFFTG
     YIKPHDFPIR QKFNKWFVSK FEYPWDRYPP FCSGTGYVFS SDVAIQVYNV SESVPFIKLE
     DVFVGLCLAK LKIRPEELHT KQTFFPGGLR FSVCRFQKIV ACHFMKPQDL LTYWQALENS
     KEQDCPAV
//