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Protein

Beta-1,3-galactosyltransferase 5

Gene

B3galt5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor. Also catalyzes the transfer of Gal to the terminal GalNAc unit of the globoside GB4, thereby synthesizing the glycolipid GB5, also known as the stage-specific embryonic antigen-3 (SSEA-3).

Pathwayi

GO - Molecular functioni

  1. galactosyltransferase activity Source: InterPro

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyltransferase 5 (EC:2.4.1.-)
Short name:
Beta-1,3-GalTase 5
Short name:
Beta3Gal-T5
Short name:
Beta3GalT5
Short name:
b3Gal-T5
Alternative name(s):
Beta-3-Gx-T5
Stage-specific embryonic antigen 3 synthase
Short name:
SSEA-3 synthase
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5
Gene namesi
Name:B3galt5
Synonyms:B3gt5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2136878. B3galt5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2518Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini26 – 308283LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. Golgi apparatus Source: MGI
  3. Golgi membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Beta-1,3-galactosyltransferase 5PRO_0000219165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9JI67.
PaxDbiQ9JI67.
PRIDEiQ9JI67.

Expressioni

Tissue specificityi

Expressed in brain and kidney.

Gene expression databases

BgeeiQ9JI67.
GenevestigatoriQ9JI67.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109431.

Structurei

3D structure databases

ProteinModelPortaliQ9JI67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG293774.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000059604.
HOVERGENiHBG094868.
InParanoidiQ9JI67.
KOiK03877.
OMAiNKWFVSK.
OrthoDBiEOG7M3J0F.
PhylomeDBiQ9JI67.
TreeFamiTF318639.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JI67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHMKTRLVY ASILMMGALC LYFSMDSFRE LPFVFKKSHG KFLQIPDIDC
60 70 80 90 100
KQKPPFLVLL VTSSHKQLAA RMAIRKTWGR ETSVQGQQVR TFFLLGTSDS
110 120 130 140 150
TEEMDATTLE SEQHRDIIQK DFKDAYFNLT LKTMMGMEWV YHFCPQTAYV
160 170 180 190 200
MKTDSDMFVN VGYLTELLLK KNKTTRFFTG YIKPHDFPIR QKFNKWFVSK
210 220 230 240 250
FEYPWDRYPP FCSGTGYVFS SDVAIQVYNV SESVPFIKLE DVFVGLCLAK
260 270 280 290 300
LKIRPEELHT KQTFFPGGLR FSVCRFQKIV ACHFMKPQDL LTYWQALENS

KEQDCPAV
Length:308
Mass (Da):35,964
Last modified:September 30, 2000 - v1
Checksum:i789073A5178825B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF254738 Genomic DNA. Translation: AAF86241.1.
BC057887 mRNA. Translation: AAH57887.1.
CCDSiCCDS28356.1.
RefSeqiNP_001116465.1. NM_001122993.1.
NP_149161.1. NM_033149.3.
XP_006523179.1. XM_006523116.2.
XP_006523180.1. XM_006523117.1.
UniGeneiMm.154783.

Genome annotation databases

EnsembliENSMUST00000099497; ENSMUSP00000097096; ENSMUSG00000074892.
ENSMUST00000113800; ENSMUSP00000109431; ENSMUSG00000074892.
GeneIDi93961.
KEGGimmu:93961.
UCSCiuc008acv.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b3GalT5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF254738 Genomic DNA. Translation: AAF86241.1.
BC057887 mRNA. Translation: AAH57887.1.
CCDSiCCDS28356.1.
RefSeqiNP_001116465.1. NM_001122993.1.
NP_149161.1. NM_033149.3.
XP_006523179.1. XM_006523116.2.
XP_006523180.1. XM_006523117.1.
UniGeneiMm.154783.

3D structure databases

ProteinModelPortaliQ9JI67.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109431.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Proteomic databases

MaxQBiQ9JI67.
PaxDbiQ9JI67.
PRIDEiQ9JI67.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099497; ENSMUSP00000097096; ENSMUSG00000074892.
ENSMUST00000113800; ENSMUSP00000109431; ENSMUSG00000074892.
GeneIDi93961.
KEGGimmu:93961.
UCSCiuc008acv.2. mouse.

Organism-specific databases

CTDi10317.
MGIiMGI:2136878. B3galt5.

Phylogenomic databases

eggNOGiNOG293774.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000059604.
HOVERGENiHBG094868.
InParanoidiQ9JI67.
KOiK03877.
OMAiNKWFVSK.
OrthoDBiEOG7M3J0F.
PhylomeDBiQ9JI67.
TreeFamiTF318639.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

NextBioi351957.
PROiQ9JI67.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JI67.
GenevestigatoriQ9JI67.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The beta1,3-galactosyltransferase beta3GalT-V is a stage-specific embryonic antigen-3 (SSEA-3) synthase."
    Zhou D., Henion T.R., Jungalwala F.B., Berger E.G., Hennet T.
    J. Biol. Chem. 275:22631-22634(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.

Entry informationi

Entry nameiB3GT5_MOUSE
AccessioniPrimary (citable) accession number: Q9JI67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2001
Last sequence update: September 30, 2000
Last modified: March 31, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.