Q9JI67 (B3GT5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,3-galactosyltransferase 5
Short name=Beta-1,3-GalTase 5
Short name=Beta3Gal-T5
Short name=Beta3GalT5
Short name=b3Gal-T5
EC=2.4.1.-

Alternative name(s):
Beta-3-Gx-T5
Stage-specific embryonic antigen 3 synthase
Short name=SSEA-3 synthase
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5

Gene names

Name:	B3galt5
Synonyms:	B3gt5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor. Also catalyzes the transfer of Gal to the terminal GalNAc unit of the globoside GB4, thereby synthesizing the glycolipid GB5, also known as the stage-specific embryonic antigen-3 (SSEA-3).
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Expressed in brain and kidney.
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Beta-1,3-galactosyltransferase 5		PRO_0000219165
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 25	18	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	26 – 308	283	Lumenal Potential
Amino acid modifications
	Glycosylation	128	1	N-linked (GlcNAc...) Potential
	Glycosylation	172	1	N-linked (GlcNAc...) Potential
	Glycosylation	229	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9JI67 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 789073A5178825B1	FASTA	308	35,964
	10 20 30 40 50 60 MAHMKTRLVY ASILMMGALC LYFSMDSFRE LPFVFKKSHG KFLQIPDIDC KQKPPFLVLL 70 80 90 100 110 120 VTSSHKQLAA RMAIRKTWGR ETSVQGQQVR TFFLLGTSDS TEEMDATTLE SEQHRDIIQK 130 140 150 160 170 180 DFKDAYFNLT LKTMMGMEWV YHFCPQTAYV MKTDSDMFVN VGYLTELLLK KNKTTRFFTG 190 200 210 220 230 240 YIKPHDFPIR QKFNKWFVSK FEYPWDRYPP FCSGTGYVFS SDVAIQVYNV SESVPFIKLE 250 260 270 280 290 300 DVFVGLCLAK LKIRPEELHT KQTFFPGGLR FSVCRFQKIV ACHFMKPQDL LTYWQALENS KEQDCPAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The beta1,3-galactosyltransferase beta3GalT-V is a stage-specific embryonic antigen-3 (SSEA-3) synthase." Zhou D., Henion T.R., Jungalwala F.B., Berger E.G., Hennet T. J. Biol. Chem. 275:22631-22634(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

b3GalT5

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF254738 Genomic DNA. Translation: AAF86241.1. BC057887 mRNA. Translation: AAH57887.1.
IPI	IPI00119954.
RefSeq	NP_001116465.1. NM_001122993.1. NP_149161.1. NM_033149.3.
UniGene	Mm.154783.
3D structure databases
ProteinModelPortal	Q9JI67.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000109431.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PaxDb	Q9JI67.
PRIDE	Q9JI67.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000099497; ENSMUSP00000097096; ENSMUSG00000074892. ENSMUST00000113800; ENSMUSP00000109431; ENSMUSG00000074892.
GeneID	93961.
KEGG	mmu:93961.
UCSC	uc008acv.2. mouse.
Organism-specific databases
CTD	10317.
MGI	MGI:2136878. B3galt5.
Phylogenomic databases
eggNOG	NOG293774.
GeneTree	ENSGT00670000097716.
HOGENOM	HOG000059604.
HOVERGEN	HBG094868.
InParanoid	Q9JI67.
KO	K03877.
OMA	EYPWDRY.
OrthoDB	EOG49ZXPQ.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Bgee	Q9JI67.
Genevestigator	Q9JI67.
GermOnline	ENSMUSG00000074892. Mus musculus.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. PTHR11214. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	351957.
SOURCE	Search...

Entry information

Entry name

B3GT5_MOUSE

Accession

Primary (citable) accession number: Q9JI67

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program