Q9JI59 (JAM2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Junctional adhesion molecule B
Short name=JAM-B

Alternative name(s):
Junctional adhesion molecule 2
Short name=JAM-2
Vascular endothelial junction-associated molecule
Short name=VE-JAM
CD_antigen=CD322

Gene names

Name:	Jam2
Synonyms:	Vejam

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the processes of lymphocyte homing to secondary lymphoid organs By similarity.
Subunit structure	Interacts with JAM3 By similarity.
Subcellular location	Cell junction › tight junction. Cell membrane; Single-pass type I membrane protein. Note: Localized at tight junctions of both epithelial and endothelial cells. Ref.2
Sequence similarities	Belongs to the immunoglobulin superfamily. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Tight junction
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of cell adhesion Inferred from genetic interaction PubMed 16093349. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell tight junction Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Ref.1
	Chain	29 – 298	270	Junctional adhesion molecule B		PRO_0000015070
Regions
	Topological domain	29 – 236	208	Extracellular Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Topological domain	258 – 298	41	Cytoplasmic Potential
	Domain	32 – 128	97	Ig-like V-type
	Domain	135 – 238	104	Ig-like C2-type
Amino acid modifications
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Disulfide bond	51 ↔ 110		Potential
	Disulfide bond	156 ↔ 214		Potential
Experimental info
	Sequence conflict	133	1	V → M in BAC26102. Ref.3
	Sequence conflict	174	1	T → H in BAC37139. Ref.3
	Sequence conflict	183	1	G → R in BAC37139. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9JI59 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1124E0F07E6CF751	FASTA	298	33,047
	10 20 30 40 50 60 MARSPQGLLM LLLLHYLIVA LDYHKANGFS ASKDHRQEVT VIEFQEAILA CKTPKKTTSS 70 80 90 100 110 120 RLEWKKVGQG VSLVYYQQAL QGDFKDRAEM IDFNIRIKNV TRSDAGEYRC EVSAPTEQGQ 130 140 150 160 170 180 NLQEDKVMLE VLVAPAVPAC EVPTSVMTGS VVELRCQDKE GNPAPEYIWF KDGTSLLGNP 190 200 210 220 230 240 KGGTHNNSSY TMNTKSGILQ FNMISKMDSG EYYCEARNSV GHRRCPGKRM QVDVLNISGI 250 260 270 280 290 IATVVVVAFV ISVCGLGTCY AQRKGYFSKE TSFQKGSPAS KVTTMSENDF KHTKSFII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Vascular endothelial junction-associated molecule, a novel member of the immunoglobulin superfamily, is localized to intercellular boundaries of endothelial cells." Palmeri D., van Zante A., Huang C.-C., Hemmerich S., Rosen S.D. J. Biol. Chem. 275:19139-19145(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-33. Strain: C57BL/6J.
[2]	"Cloning of JAM-2 and JAM-3: an emerging junctional adhesion molecular family?" Aurrand-Lions M.A., Duncan L., Du Pasquier L., Imhof B.A. Curr. Top. Microbiol. Immunol. 251:91-98(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head, Medulla oblongata and Skin.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
[6]	"Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response." Muller W.A. Trends Immunol. 24:327-334(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF255911 mRNA. Translation: AAF81224.1. AJ291757 mRNA. Translation: CAC20699.1. AK010616 mRNA. Translation: BAB27064.1. AK013914 mRNA. Translation: BAB29053.1. AK028757 mRNA. Translation: BAC26102.1. AK078128 mRNA. Translation: BAC37139.1. CT027693, AC164162 Genomic DNA. Translation: CAO78081.1. BC028778 mRNA. Translation: AAH28778.1.
IPI	IPI00622727.
RefSeq	NP_076333.3. NM_023844.5.
UniGene	Mm.41758.
3D structure databases
ProteinModelPortal	Q9JI59.
SMR	Q9JI59. Positions 36-239.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000109833.
PTM databases
PhosphoSite	Q9JI59.
Proteomic databases
PaxDb	Q9JI59.
PRIDE	Q9JI59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000114195; ENSMUSP00000109833; ENSMUSG00000053062.
GeneID	67374.
KEGG	mmu:67374.
UCSC	uc007ztg.1. mouse.
Organism-specific databases
CTD	58494.
MGI	MGI:1933820. Jam2.
Phylogenomic databases
eggNOG	NOG132289.
GeneTree	ENSGT00670000097525.
HOGENOM	HOG000247041.
HOVERGEN	HBG000518.
InParanoid	A6X955.
KO	K06735.
OMA	SCEVPNS.
OrthoDB	EOG4TQMB0.
Gene expression databases
ArrayExpress	Q9JI59.
Bgee	Q9JI59.
CleanEx	MM_JAM2.
Genevestigator	Q9JI59.
GermOnline	ENSMUSG00000053062. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR013106. Ig_V-set. [Graphical view]
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. SM00408. IGc2. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	324388.
SOURCE	Search...

Entry information

Entry name

JAM2_MOUSE

Accession

Primary (citable) accession number: Q9JI59
Secondary accession number(s): A6X955, Q8C5K9, Q8CE95

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program