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Protein

Junctional adhesion molecule B

Gene

Jam2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the processes of lymphocyte homing to secondary lymphoid organs.By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI

GO - Biological processi

  • negative regulation of cell adhesion Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_278886. Cell surface interactions at the vascular wall.
REACT_319261. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Junctional adhesion molecule B
Short name:
JAM-B
Alternative name(s):
Junctional adhesion molecule 2
Short name:
JAM-2
Vascular endothelial junction-associated molecule
Short name:
VE-JAM
CD_antigen: CD322
Gene namesi
Name:Jam2
Synonyms:Vejam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1933820. Jam2.

Subcellular locationi

  • Cell junctiontight junction 1 Publication
  • Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

  • Note: Localized at tight junctions of both epithelial and endothelial cells.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 236208ExtracellularSequence AnalysisAdd
BLAST
Transmembranei237 – 25721HelicalSequence AnalysisAdd
BLAST
Topological domaini258 – 29841CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28281 PublicationAdd
BLAST
Chaini29 – 298270Junctional adhesion molecule BPRO_0000015070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi51 ↔ 110PROSITE-ProRule annotation
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi156 ↔ 214PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9JI59.
PaxDbiQ9JI59.
PRIDEiQ9JI59.

PTM databases

PhosphoSiteiQ9JI59.

Expressioni

Gene expression databases

BgeeiQ9JI59.
CleanExiMM_JAM2.
ExpressionAtlasiQ9JI59. baseline and differential.
GenevisibleiQ9JI59. MM.

Interactioni

Subunit structurei

Interacts with JAM3.By similarity

Protein-protein interaction databases

DIPiDIP-61078N.
STRINGi10090.ENSMUSP00000109833.

Structurei

3D structure databases

ProteinModelPortaliQ9JI59.
SMRiQ9JI59. Positions 36-239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 12897Ig-like V-typeAdd
BLAST
Domaini135 – 238104Ig-like C2-typeAdd
BLAST

Sequence similaritiesi

Belongs to the immunoglobulin superfamily.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG132289.
GeneTreeiENSGT00730000110678.
HOGENOMiHOG000247041.
HOVERGENiHBG000518.
InParanoidiQ9JI59.
KOiK06735.
OMAiSCEVPNS.
OrthoDBiEOG7MH0Z1.
PhylomeDBiQ9JI59.
TreeFamiTF331459.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07679. I-set. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JI59-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSPQGLLM LLLLHYLIVA LDYHKANGFS ASKDHRQEVT VIEFQEAILA
60 70 80 90 100
CKTPKKTTSS RLEWKKVGQG VSLVYYQQAL QGDFKDRAEM IDFNIRIKNV
110 120 130 140 150
TRSDAGEYRC EVSAPTEQGQ NLQEDKVMLE VLVAPAVPAC EVPTSVMTGS
160 170 180 190 200
VVELRCQDKE GNPAPEYIWF KDGTSLLGNP KGGTHNNSSY TMNTKSGILQ
210 220 230 240 250
FNMISKMDSG EYYCEARNSV GHRRCPGKRM QVDVLNISGI IATVVVVAFV
260 270 280 290
ISVCGLGTCY AQRKGYFSKE TSFQKGSPAS KVTTMSENDF KHTKSFII
Length:298
Mass (Da):33,047
Last modified:October 1, 2000 - v1
Checksum:i1124E0F07E6CF751
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331V → M in BAC26102 (PubMed:16141072).Curated
Sequence conflicti174 – 1741T → H in BAC37139 (PubMed:16141072).Curated
Sequence conflicti183 – 1831G → R in BAC37139 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF255911 mRNA. Translation: AAF81224.1.
AJ291757 mRNA. Translation: CAC20699.1.
AK010616 mRNA. Translation: BAB27064.1.
AK013914 mRNA. Translation: BAB29053.1.
AK028757 mRNA. Translation: BAC26102.1.
AK078128 mRNA. Translation: BAC37139.1.
CT027693, AC164162 Genomic DNA. Translation: CAO78081.1.
BC028778 mRNA. Translation: AAH28778.1.
CCDSiCCDS37381.1.
RefSeqiNP_076333.3. NM_023844.5.
UniGeneiMm.41758.

Genome annotation databases

EnsembliENSMUST00000114195; ENSMUSP00000109833; ENSMUSG00000053062.
GeneIDi67374.
KEGGimmu:67374.
UCSCiuc007ztg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF255911 mRNA. Translation: AAF81224.1.
AJ291757 mRNA. Translation: CAC20699.1.
AK010616 mRNA. Translation: BAB27064.1.
AK013914 mRNA. Translation: BAB29053.1.
AK028757 mRNA. Translation: BAC26102.1.
AK078128 mRNA. Translation: BAC37139.1.
CT027693, AC164162 Genomic DNA. Translation: CAO78081.1.
BC028778 mRNA. Translation: AAH28778.1.
CCDSiCCDS37381.1.
RefSeqiNP_076333.3. NM_023844.5.
UniGeneiMm.41758.

3D structure databases

ProteinModelPortaliQ9JI59.
SMRiQ9JI59. Positions 36-239.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61078N.
STRINGi10090.ENSMUSP00000109833.

PTM databases

PhosphoSiteiQ9JI59.

Proteomic databases

MaxQBiQ9JI59.
PaxDbiQ9JI59.
PRIDEiQ9JI59.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114195; ENSMUSP00000109833; ENSMUSG00000053062.
GeneIDi67374.
KEGGimmu:67374.
UCSCiuc007ztg.2. mouse.

Organism-specific databases

CTDi58494.
MGIiMGI:1933820. Jam2.

Phylogenomic databases

eggNOGiNOG132289.
GeneTreeiENSGT00730000110678.
HOGENOMiHOG000247041.
HOVERGENiHBG000518.
InParanoidiQ9JI59.
KOiK06735.
OMAiSCEVPNS.
OrthoDBiEOG7MH0Z1.
PhylomeDBiQ9JI59.
TreeFamiTF331459.

Enzyme and pathway databases

ReactomeiREACT_278886. Cell surface interactions at the vascular wall.
REACT_319261. Integrin cell surface interactions.

Miscellaneous databases

NextBioi324388.
PROiQ9JI59.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JI59.
CleanExiMM_JAM2.
ExpressionAtlasiQ9JI59. baseline and differential.
GenevisibleiQ9JI59. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07679. I-set. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Vascular endothelial junction-associated molecule, a novel member of the immunoglobulin superfamily, is localized to intercellular boundaries of endothelial cells."
    Palmeri D., van Zante A., Huang C.-C., Hemmerich S., Rosen S.D.
    J. Biol. Chem. 275:19139-19145(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-33.
    Strain: C57BL/6J.
  2. "Cloning of JAM-2 and JAM-3: an emerging junctional adhesion molecular family?"
    Aurrand-Lions M.A., Duncan L., Du Pasquier L., Imhof B.A.
    Curr. Top. Microbiol. Immunol. 251:91-98(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head, Medulla oblongata and Skin.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  6. "Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response."
    Muller W.A.
    Trends Immunol. 24:327-334(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, NOMENCLATURE.

Entry informationi

Entry nameiJAM2_MOUSE
AccessioniPrimary (citable) accession number: Q9JI59
Secondary accession number(s): A6X955, Q8C5K9, Q8CE95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 1, 2000
Last modified: July 22, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.