Vesicle transport through interaction with t-SNAREs homolog 1A

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

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Protein-protein interaction databases

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Molecule processing

Regions

Natural variations

Secondary structure

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3D structure databases

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Q9JI51 (VTI1A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 81. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
Vesicle transport through interaction with t-SNAREs homolog 1A

Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 2
Vti1-rp2

Gene names

Name:	Vti1a
Synonyms:	Vti1l2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Function	V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2 By similarity. May be concerned with increased secretion of cytokines associated with cellular senescence. Ref.3
Subunit structure	Interacts with distinct SNARE complexes that contain either STX5 or STX6 By similarity. Interacts with NAPA and, to a lesser extent, with NAPG By similarity. Also identified in a complex containing STX13 and VAMP4.
Subcellular location	Membrane; Single-pass type IV membrane protein. Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass type IV membrane protein. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein. Note: Mainly associated with the Golgi apparatus. Enriched in small synaptic vesicles and clathrin-coated vesicles from nerve terminals. Isoform 1/VTI1A localizes to the cell bodies of hippocampal neurons as well as to nerve terminals. Ref.2
Tissue specificity	Specifically expressed in the neuronal tissues cerebellum, cortex and hippocampus. Isoform 1/VTI1A is expressed in the same neuronal tissues but also in lung, liver, kidney and spleen.
Sequence similarities	Belongs to the VTI1 family.

Keywords
Biological process	Protein transport Transport
Cellular component	Cell junction Cytoplasmic vesicle Golgi apparatus Membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from direct assay. Source: RGD intracellular protein transport Inferred from electronic annotation. Source: InterPro retrograde transport, endosome to Golgi Inferred from sequence or structural similarity. Source: UniProtKB vesicle fusion with Golgi apparatus Inferred from direct assay. Source: RGD
Cellular component	SNARE complex Inferred from direct assay Ref.1. Source: RGD cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from direct assay Ref.1. Source: RGD synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 224

224

Vesicle transport through interaction with t-SNAREs homolog 1A

PRO_0000218227

Topological domain

1 – 199

199

Cytoplasmic Potential

Transmembrane

200 – 220

Helical; Anchor for type IV membrane protein; Potential

Topological domain

221 – 224

Vesicular Potential

Coiled coil

31 – 92

Potential

Coiled coil

106 – 185

Potential

Alternative sequence

115 – 121

Missing in isoform 1.

VSP_006754

224

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform 2 (VTI1A-beta) [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 508CAC274ED055C4 Show »	FASTA	224	26,043
10 20 30 40 50 60 MSADFEGYEQ DFAVLTAEIT SKISRVPRLP PDEKKQMVAN VEKQLEEARE LLEQMDLEVR 70 80 90 100 110 120 EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR NELLGDAGNS SENQLIKLRE 130 140 150 160 170 180 ERAHLLDNTE RLERSSRRLE AGYQIAVETE QIGQEMLENL SHDRERIQRA RERLRETDAN 190 200 210 220 LGKSSRILTG MLRRIIQNRI LLVILGIIVV ITILTAITFF VRGH « Hide
Isoform 1 (VTI1A) [UniParc]. Checksum: CE489B1581256543 Show »	FASTA	217	25,161
10 20 30 40 50 60 MSADFEGYEQ DFAVLTAEIT SKISRVPRLP PDEKKQMVAN VEKQLEEARE LLEQMDLEVR 70 80 90 100 110 120 EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR NELLGDAGNS SENQRAHLLD 130 140 150 160 170 180 NTERLERSSR RLEAGYQIAV ETEQIGQEML ENLSHDRERI QRARERLRET DANLGKSSRI 190 200 210 LTGMLRRIIQ NRILLVILGI IVVITILTAI TFFVRGH « Hide

[1]	"The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex." Antonin W., Riedel D., Fischer von Mollard G. J. Neurosci. 20:5724-5732(2000) [PubMed: 10908612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: Sprague-Dawley. Tissue: Cerebellum and Lung.
[2]	"A 29-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (Vti1-rp2) implicated in protein trafficking in the secretory pathway." Xu Y., Wong S.H., Tang B.L., Subramaniam V.N., Zhang T., Hong W. J. Biol. Chem. 273:21783-21789(1998) [PubMed: 9705316] [Abstract] Cited for: SUBCELLULAR LOCATION.
[3]	"Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies." Zwilling D., Cypionka A., Pohl W.H., Fasshauer D., Walla P.J., Wahl M.C., Jahn R. EMBO J. 26:9-18(2007) [PubMed: 17159904] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 122-199 IN COMPLEX WITH STX6; STX13; VTI1A AND VAMP4, FUNCTION.
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

AF262222 mRNA. Translation: AAF97791.1.
AF262221 mRNA. Translation: AAF97790.1.

IPI

IPI00199945.
IPI00324090.

RefSeq

NP_075589.1. NM_023101.1.

UniGene

Rn.154408.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2NPS	X-ray	2.50	C	122-199	[»]

ProteinModelPortal

Q9JI51.

SMR

Q9JI51. Positions 8-94, 122-197.

ModBase

Search...

MINT

MINT-5225883.

STRING

Q9JI51.

PRIDE

Q9JI51.

StructuralBiologyKnowledgebase

Search...

GeneID

65277.

KEGG

rno:65277.

CTD

143187.

RGD

621490. Vti1a.

GeneTree

ENSGT00530000063466.

HOVERGEN

HBG104027.

PhylomeDB

Q9JI51.

Genevestigator

Q9JI51.

InterPro

IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]

K08493.

Pfam

PF05008. V-SNARE. 1 hit.
[Graphical view]

SMART

SM00397. t_SNARE. 1 hit.
[Graphical view]

SUPFAM

SSF47661. t-snare. 1 hit.

ProtoNet

Search...

NextBio

614244.

Entry name

VTI1A_RAT

Accession

Primary (citable) accession number: Q9JI51
Secondary accession number(s): Q9JI52

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q9JI51-1)

Also known as: VTI1A-beta;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q9JI51-2)

Also known as: VTI1A;

The sequence of this isoform differs from the canonical sequence as follows:
115-121: Missing.