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Protein

Vesicle transport through interaction with t-SNAREs homolog 1A

Gene

Vti1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2 (By similarity). May be concerned with increased secretion of cytokines associated with cellular senescence.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle transport through interaction with t-SNAREs homolog 1A
Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 2
Vti1-rp2
Gene namesi
Name:Vti1a
Synonyms:Vti1l2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621490 Vti1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 199CytoplasmicSequence analysisAdd BLAST199
Transmembranei200 – 220Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini221 – 224VesicularSequence analysis4

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002182271 – 224Vesicle transport through interaction with t-SNAREs homolog 1AAdd BLAST224

Proteomic databases

PaxDbiQ9JI51
PRIDEiQ9JI51

PTM databases

PhosphoSitePlusiQ9JI51

Expressioni

Tissue specificityi

Specifically expressed in the neuronal tissues cerebellum, cortex and hippocampus. Isoform 1/VTI1A is expressed in the same neuronal tissues but also in lung, liver, kidney and spleen.

Interactioni

Subunit structurei

Interacts with distinct SNARE complexes that contain either STX5 or STX6 (By similarity). Interacts with NAPA and, to a lesser extent, with NAPG (By similarity). Identified in a complex containing STX6, STX12, VAMP4 and VTI1A.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Syt11Q9R0N32EBI-7573650,EBI-647443From Mus musculus.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249335, 1 interactor
CORUMiQ9JI51
IntActiQ9JI51, 4 interactors
MINTiQ9JI51
STRINGi10116.ENSRNOP00000058953

Structurei

Secondary structure

1224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi124 – 136Combined sources13
Helixi138 – 194Combined sources57

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NPSX-ray2.50C122-199[»]
ProteinModelPortaliQ9JI51
SMRiQ9JI51
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JI51

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili31 – 92Sequence analysisAdd BLAST62
Coiled coili106 – 185Sequence analysisAdd BLAST80

Sequence similaritiesi

Belongs to the VTI1 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1666 Eukaryota
ENOG4111J90 LUCA
HOGENOMiHOG000116573
HOVERGENiHBG104027
InParanoidiQ9JI51
KOiK08493
PhylomeDBiQ9JI51

Family and domain databases

Gene3Di1.20.58.400, 1 hit
InterProiView protein in InterPro
IPR027027 GOSR2/Membrin/Bos1
IPR010989 SNARE
IPR000727 T_SNARE_dom
IPR038407 v-SNARE_N_sf
IPR007705 Vesicle_trsprt_v-SNARE_N
PfamiView protein in Pfam
PF05008 V-SNARE, 1 hit
PIRSFiPIRSF028865 Membrin-2, 1 hit
SMARTiView protein in SMART
SM00397 t_SNARE, 1 hit
SUPFAMiSSF47661 SSF47661, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: Q9JI51-1) [UniParc]FASTAAdd to basket
Also known as: VTI1A-beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSADFEGYEQ DFAVLTAEIT SKISRVPRLP PDEKKQMVAN VEKQLEEARE
60 70 80 90 100
LLEQMDLEVR EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR
110 120 130 140 150
NELLGDAGNS SENQLIKLRE ERAHLLDNTE RLERSSRRLE AGYQIAVETE
160 170 180 190 200
QIGQEMLENL SHDRERIQRA RERLRETDAN LGKSSRILTG MLRRIIQNRI
210 220
LLVILGIIVV ITILTAITFF VRGH
Length:224
Mass (Da):26,043
Last modified:October 1, 2000 - v1
Checksum:i508CAC274ED055C4
GO
Isoform 1 (identifier: Q9JI51-2) [UniParc]FASTAAdd to basket
Also known as: VTI1A

The sequence of this isoform differs from the canonical sequence as follows:
     115-121: Missing.

Show »
Length:217
Mass (Da):25,161
Checksum:iCE489B1581256543
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006754115 – 121Missing in isoform 1. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF262222 mRNA Translation: AAF97791.1
AF262221 mRNA Translation: AAF97790.1
RefSeqiNP_075589.1, NM_023101.1 [Q9JI51-2]
UniGeneiRn.154408

Genome annotation databases

GeneIDi65277
KEGGirno:65277
UCSCiRGD:621490 rat [Q9JI51-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVTI1A_RAT
AccessioniPrimary (citable) accession number: Q9JI51
Secondary accession number(s): Q9JI52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health