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Protein

Vesicle transport through interaction with t-SNAREs homolog 1A

Gene

Vti1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2 (By similarity). May be concerned with increased secretion of cytokines associated with cellular senescence.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle transport through interaction with t-SNAREs homolog 1A
Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 2
Vti1-rp2
Gene namesi
Name:Vti1a
Synonyms:Vti1l2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621490. Vti1a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 199199CytoplasmicSequence analysisAdd
BLAST
Transmembranei200 – 22021Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini221 – 2244VesicularSequence analysis

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • clathrin-coated vesicle Source: RGD
  • clathrin-coated vesicle membrane Source: UniProtKB-SubCell
  • cytosol Source: GOC
  • endoplasmic reticulum membrane Source: GO_Central
  • endosome Source: ParkinsonsUK-UCL
  • ER to Golgi transport vesicle membrane Source: GO_Central
  • Golgi apparatus Source: GO_Central
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • late endosome membrane Source: GO_Central
  • neuronal cell body Source: ParkinsonsUK-UCL
  • neuron projection terminus Source: ParkinsonsUK-UCL
  • perinuclear region of cytoplasm Source: ParkinsonsUK-UCL
  • SNARE complex Source: ParkinsonsUK-UCL
  • synaptic vesicle Source: ParkinsonsUK-UCL
  • synaptic vesicle membrane Source: UniProtKB-SubCell
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Vesicle transport through interaction with t-SNAREs homolog 1APRO_0000218227Add
BLAST

Proteomic databases

PaxDbiQ9JI51.
PRIDEiQ9JI51.

PTM databases

PhosphoSiteiQ9JI51.

Expressioni

Tissue specificityi

Specifically expressed in the neuronal tissues cerebellum, cortex and hippocampus. Isoform 1/VTI1A is expressed in the same neuronal tissues but also in lung, liver, kidney and spleen.

Interactioni

Subunit structurei

Interacts with distinct SNARE complexes that contain either STX5 or STX6 (By similarity). Interacts with NAPA and, to a lesser extent, with NAPG (By similarity). Identified in a complex containing STX6, STX12, VAMP4 and VTI1A.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Syt11Q9R0N32EBI-7573650,EBI-647443From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249335. 1 interaction.
IntActiQ9JI51. 4 interactions.
MINTiMINT-5225883.
STRINGi10116.ENSRNOP00000058953.

Structurei

Secondary structure

1
224
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi124 – 13613Combined sources
Helixi138 – 19457Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2NPSX-ray2.50C122-199[»]
ProteinModelPortaliQ9JI51.
SMRiQ9JI51. Positions 8-94, 122-197.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JI51.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili31 – 9262Sequence analysisAdd
BLAST
Coiled coili106 – 18580Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the VTI1 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1666. Eukaryota.
ENOG4111J90. LUCA.
HOGENOMiHOG000116573.
HOVERGENiHBG104027.
InParanoidiQ9JI51.
KOiK08493.
PhylomeDBiQ9JI51.

Family and domain databases

InterProiIPR027027. GOSR2/Membrin/Bos1.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamiPF05008. V-SNARE. 1 hit.
[Graphical view]
PIRSFiPIRSF028865. Membrin-2. 1 hit.
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: Q9JI51-1) [UniParc]FASTAAdd to basket
Also known as: VTI1A-beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSADFEGYEQ DFAVLTAEIT SKISRVPRLP PDEKKQMVAN VEKQLEEARE
60 70 80 90 100
LLEQMDLEVR EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR
110 120 130 140 150
NELLGDAGNS SENQLIKLRE ERAHLLDNTE RLERSSRRLE AGYQIAVETE
160 170 180 190 200
QIGQEMLENL SHDRERIQRA RERLRETDAN LGKSSRILTG MLRRIIQNRI
210 220
LLVILGIIVV ITILTAITFF VRGH
Length:224
Mass (Da):26,043
Last modified:October 1, 2000 - v1
Checksum:i508CAC274ED055C4
GO
Isoform 1 (identifier: Q9JI51-2) [UniParc]FASTAAdd to basket
Also known as: VTI1A

The sequence of this isoform differs from the canonical sequence as follows:
     115-121: Missing.

Show »
Length:217
Mass (Da):25,161
Checksum:iCE489B1581256543
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei115 – 1217Missing in isoform 1. 1 PublicationVSP_006754

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF262222 mRNA. Translation: AAF97791.1.
AF262221 mRNA. Translation: AAF97790.1.
RefSeqiNP_075589.1. NM_023101.1. [Q9JI51-2]
UniGeneiRn.154408.

Genome annotation databases

GeneIDi65277.
KEGGirno:65277.
UCSCiRGD:621490. rat. [Q9JI51-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF262222 mRNA. Translation: AAF97791.1.
AF262221 mRNA. Translation: AAF97790.1.
RefSeqiNP_075589.1. NM_023101.1. [Q9JI51-2]
UniGeneiRn.154408.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2NPSX-ray2.50C122-199[»]
ProteinModelPortaliQ9JI51.
SMRiQ9JI51. Positions 8-94, 122-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249335. 1 interaction.
IntActiQ9JI51. 4 interactions.
MINTiMINT-5225883.
STRINGi10116.ENSRNOP00000058953.

PTM databases

PhosphoSiteiQ9JI51.

Proteomic databases

PaxDbiQ9JI51.
PRIDEiQ9JI51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65277.
KEGGirno:65277.
UCSCiRGD:621490. rat. [Q9JI51-1]

Organism-specific databases

CTDi143187.
RGDi621490. Vti1a.

Phylogenomic databases

eggNOGiKOG1666. Eukaryota.
ENOG4111J90. LUCA.
HOGENOMiHOG000116573.
HOVERGENiHBG104027.
InParanoidiQ9JI51.
KOiK08493.
PhylomeDBiQ9JI51.

Miscellaneous databases

EvolutionaryTraceiQ9JI51.
PROiQ9JI51.

Family and domain databases

InterProiIPR027027. GOSR2/Membrin/Bos1.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamiPF05008. V-SNARE. 1 hit.
[Graphical view]
PIRSFiPIRSF028865. Membrin-2. 1 hit.
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVTI1A_RAT
AccessioniPrimary (citable) accession number: Q9JI51
Secondary accession number(s): Q9JI52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.