Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diphosphoinositol polyphosphate phosphohydrolase 1

Gene

Nudt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. InsP6 (inositol hexakisphophate) is not a substrate. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate (By similarity). Acts as a negative regulator of the ERK1/2 pathway.By similarity

Catalytic activityi

Diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by fluoride and InsP6.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10SubstrateBy similarity1
Binding sitei41SubstrateBy similarity1
Metal bindingi50Magnesium 1; via carbonyl oxygenBy similarity1
Metal bindingi66Magnesium 2By similarity1
Metal bindingi66Magnesium 3By similarity1
Active sitei69Proton acceptorBy similarity1
Metal bindingi70Magnesium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1855167. Synthesis of pyrophosphates in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphosphoinositol polyphosphate phosphohydrolase 1 (EC:3.6.1.52)
Short name:
DIPP-1
Short name:
muDIPP1
Alternative name(s):
Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 1 (EC:3.6.1.-)
Nucleoside diphosphate-linked moiety X motif 3
Short name:
Nudix motif 3
Gene namesi
Name:Nudt3
Synonyms:Dipp, Dipp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1928484. Nudt3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi70E → Q: Loss of enzyme activity, but retains ability to regulate the ERK1/2 pathway. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000570561 – 168Diphosphoinositol polyphosphate phosphohydrolase 1Add BLAST168

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9JI46.
MaxQBiQ9JI46.
PaxDbiQ9JI46.
PRIDEiQ9JI46.

PTM databases

iPTMnetiQ9JI46.
PhosphoSitePlusiQ9JI46.

Expressioni

Tissue specificityi

Present in heart, lung, liver and spleen (at protein level). Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000024213.
ExpressionAtlasiQ9JI46. baseline and differential.
GenevisibleiQ9JI46. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ9JI46. 1 interactor.
MINTiMINT-4129743.
STRINGi10090.ENSMUSP00000025050.

Structurei

3D structure databases

ProteinModelPortaliQ9JI46.
SMRiQ9JI46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 144Nudix hydrolasePROSITE-ProRule annotationAdd BLAST128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 20Substrate bindingBy similarity3
Regioni89 – 91Substrate bindingBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi51 – 72Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family. DIPP subfamily.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2839. Eukaryota.
COG0494. LUCA.
GeneTreeiENSGT00390000012928.
HOGENOMiHOG000237336.
HOVERGENiHBG053341.
InParanoidiQ9JI46.
KOiK07766.
OMAiLQESCLT.
OrthoDBiEOG091G0TH6.
PhylomeDBiQ9JI46.
TreeFamiTF106349.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JI46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKLKSNQTR TYDGDGYKKR AACLCFRSES EEEVLLVSSS RHPDRWIVPG
60 70 80 90 100
GGMEPEEEPS VAAVREVCEE AGVKGTLGRL VGIFENQERK HRTYVYVLIV
110 120 130 140 150
TEVLEDWEDS VNIGRKREWF KIEDAIKVLQ CHKPVQASYF ETLRQGYPAN
160
NGTPVVPTTY SSSVSGIR
Length:168
Mass (Da):19,030
Last modified:October 1, 2000 - v1
Checksum:iE543BE5CBE520910
GO

Sequence cautioni

The sequence AAH57331 differs from that shown. Chimeric at the C-terminus.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264064 mRNA. Translation: AAF74761.1.
CT027568 Genomic DNA. Translation: CAQ52215.1.
BC016534 mRNA. Translation: AAH16534.1.
BC046805 mRNA. Translation: AAH46805.1.
BC057331 mRNA. Translation: AAH57331.1. Sequence problems.
AK079658 mRNA. Translation: BAC37717.1.
CCDSiCCDS28566.1.
RefSeqiNP_062811.1. NM_019837.2.
UniGeneiMm.144699.
Mm.32556.
Mm.473041.

Genome annotation databases

EnsembliENSMUST00000025050; ENSMUSP00000025050; ENSMUSG00000024213.
GeneIDi56409.
KEGGimmu:56409.
UCSCiuc008bpg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264064 mRNA. Translation: AAF74761.1.
CT027568 Genomic DNA. Translation: CAQ52215.1.
BC016534 mRNA. Translation: AAH16534.1.
BC046805 mRNA. Translation: AAH46805.1.
BC057331 mRNA. Translation: AAH57331.1. Sequence problems.
AK079658 mRNA. Translation: BAC37717.1.
CCDSiCCDS28566.1.
RefSeqiNP_062811.1. NM_019837.2.
UniGeneiMm.144699.
Mm.32556.
Mm.473041.

3D structure databases

ProteinModelPortaliQ9JI46.
SMRiQ9JI46.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JI46. 1 interactor.
MINTiMINT-4129743.
STRINGi10090.ENSMUSP00000025050.

PTM databases

iPTMnetiQ9JI46.
PhosphoSitePlusiQ9JI46.

Proteomic databases

EPDiQ9JI46.
MaxQBiQ9JI46.
PaxDbiQ9JI46.
PRIDEiQ9JI46.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025050; ENSMUSP00000025050; ENSMUSG00000024213.
GeneIDi56409.
KEGGimmu:56409.
UCSCiuc008bpg.2. mouse.

Organism-specific databases

CTDi11165.
MGIiMGI:1928484. Nudt3.

Phylogenomic databases

eggNOGiKOG2839. Eukaryota.
COG0494. LUCA.
GeneTreeiENSGT00390000012928.
HOGENOMiHOG000237336.
HOVERGENiHBG053341.
InParanoidiQ9JI46.
KOiK07766.
OMAiLQESCLT.
OrthoDBiEOG091G0TH6.
PhylomeDBiQ9JI46.
TreeFamiTF106349.

Enzyme and pathway databases

ReactomeiR-MMU-1855167. Synthesis of pyrophosphates in the cytosol.

Miscellaneous databases

ChiTaRSiNudt3. mouse.
PROiQ9JI46.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024213.
ExpressionAtlasiQ9JI46. baseline and differential.
GenevisibleiQ9JI46. MM.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUDT3_MOUSE
AccessioniPrimary (citable) accession number: Q9JI46
Secondary accession number(s): B2KF68, Q6PG02, Q8BV71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.