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Protein

tRNA pseudouridine(38/39) synthase

Gene

Pus3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs. Also acts on position 38, but much less efficiently.1 Publication

Catalytic activityi

tRNA uridine(38/39) = tRNA pseudouridine(38/39).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei119NucleophileBy similarity1
Binding sitei196SubstrateBy similarity1

GO - Molecular functioni

  • pseudouridine synthase activity Source: MGI
  • RNA binding Source: InterPro

GO - Biological processi

  • tRNA pseudouridine synthesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BRENDAi4.2.1.70. 3474.
5.4.99.12. 3474.
5.4.99.45. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine(38/39) synthase (EC:5.4.99.45)
Alternative name(s):
tRNA pseudouridine synthase 3
tRNA pseudouridylate synthase 3
tRNA-uridine isomerase 3
Gene namesi
Name:Pus3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1914299. Pus3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000575212 – 481tRNA pseudouridine(38/39) synthaseAdd BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9JI38.
MaxQBiQ9JI38.
PaxDbiQ9JI38.
PeptideAtlasiQ9JI38.
PRIDEiQ9JI38.

PTM databases

PhosphoSitePlusiQ9JI38.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032103.
CleanExiMM_PUS3.
ExpressionAtlasiQ9JI38. baseline and differential.
GenevisibleiQ9JI38. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034615.

Structurei

3D structure databases

ProteinModelPortaliQ9JI38.
SMRiQ9JI38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2554. Eukaryota.
COG0101. LUCA.
GeneTreeiENSGT00510000047444.
HOGENOMiHOG000199032.
HOVERGENiHBG053769.
InParanoidiQ9JI38.
KOiK01855.
OMAiKRVCVDT.
OrthoDBiEOG091G05MF.
PhylomeDBiQ9JI38.
TreeFamiTF314428.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JI38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENTDRNQI EKLLNRVKEL EQEVERLKKK KEQANNIKDS SIRENSLGSG
60 70 80 90 100
KAKRAFDFSA HGRRHVALKI AYLGWGYQGF ASQENTSNTI EEKLFEALTK
110 120 130 140 150
TRLVESRQTS NYHRCGRTDK GVSAFGQVIS LDLRSQFPTS RDSEDSNLKH
160 170 180 190 200
EADDLAKEIR YTHILNRVLP ADIRVLAWAP VEPSFSARFS CLERTYRYFF
210 220 230 240 250
PRADLDIATM NYAAQKYVGT HDFRNLCKMD VANGVINFQR TILCAQVQLV
260 270 280 290 300
AQSPGEERRQ EPFQLCQFEV IGQAFLYHQV RCMMAILFLI GQGMEKPEII
310 320 330 340 350
DELLNIQKNP QKPQYSMAVE FPLVLYDCKF ENTKWIYDHE VQEFNVTHLQ
360 370 380 390 400
QLWANHAVKT HMLYSMLQGL DSVMVTCAAG TKMDEATEWR NIQPPVIKHT
410 420 430 440 450
SAFVEGVKMR TYKPLMDRPK CQGLESRIRH FVSRGRIEHP HLLHKEEIKA
460 470 480
RRDCADKEEN TVVENPSKRV CIIDAEINSI A
Length:481
Mass (Da):55,546
Last modified:September 27, 2004 - v2
Checksum:i32B6B59984AF830D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti237N → D in AAF91402 (PubMed:11027153).Curated1
Sequence conflicti384D → G in BAC37536 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF266505 mRNA. Translation: AAF91402.1.
AK079095 mRNA. Translation: BAC37536.1.
BC029253 mRNA. Translation: AAH29253.1.
CCDSiCCDS22965.1.
RefSeqiNP_075781.3. NM_023292.4.
XP_011240888.1. XM_011242586.2.
UniGeneiMm.425574.
Mm.482285.

Genome annotation databases

EnsembliENSMUST00000034615; ENSMUSP00000034615; ENSMUSG00000032103.
GeneIDi67049.
KEGGimmu:67049.
UCSCiuc009oth.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF266505 mRNA. Translation: AAF91402.1.
AK079095 mRNA. Translation: BAC37536.1.
BC029253 mRNA. Translation: AAH29253.1.
CCDSiCCDS22965.1.
RefSeqiNP_075781.3. NM_023292.4.
XP_011240888.1. XM_011242586.2.
UniGeneiMm.425574.
Mm.482285.

3D structure databases

ProteinModelPortaliQ9JI38.
SMRiQ9JI38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034615.

PTM databases

PhosphoSitePlusiQ9JI38.

Proteomic databases

EPDiQ9JI38.
MaxQBiQ9JI38.
PaxDbiQ9JI38.
PeptideAtlasiQ9JI38.
PRIDEiQ9JI38.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034615; ENSMUSP00000034615; ENSMUSG00000032103.
GeneIDi67049.
KEGGimmu:67049.
UCSCiuc009oth.2. mouse.

Organism-specific databases

CTDi83480.
MGIiMGI:1914299. Pus3.

Phylogenomic databases

eggNOGiKOG2554. Eukaryota.
COG0101. LUCA.
GeneTreeiENSGT00510000047444.
HOGENOMiHOG000199032.
HOVERGENiHBG053769.
InParanoidiQ9JI38.
KOiK01855.
OMAiKRVCVDT.
OrthoDBiEOG091G05MF.
PhylomeDBiQ9JI38.
TreeFamiTF314428.

Enzyme and pathway databases

BRENDAi4.2.1.70. 3474.
5.4.99.12. 3474.
5.4.99.45. 3474.

Miscellaneous databases

PROiQ9JI38.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032103.
CleanExiMM_PUS3.
ExpressionAtlasiQ9JI38. baseline and differential.
GenevisibleiQ9JI38. MM.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPUS3_MOUSE
AccessioniPrimary (citable) accession number: Q9JI38
Secondary accession number(s): Q8BVA6, Q8K0Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.