Reviewed, UniProtKB/Swiss-Prot Q9JI33 (NET4_MOUSE)

Names and origin

Protein names

Recommended name:
    Netrin-4
Alternative name(s):
    Beta-netrin

Gene names

Name:

Ntn4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play an important role in neural, kidney and vascular development. Promotes neurite elongation from olfactory bulb explants. Ref.2
Subunit structure	May form a homodimer.
Subcellular location	Secreted › extracellular space › extracellular matrix › basement membrane. Note: Major component.
Tissue specificity	Expressed in kidney, liver, heart, ovary, testis, retina, brain, olfactory bulb, and widely expressed in embryo. Ref.2 Ref.1
Sequence similarities	Contains 3 laminin EGF-like domains. Contains 1 laminin N-terminal domain. Contains 1 NTR domain.

Ontologies

Keywords
Cellular component	Basement membrane Extracellular matrix Secreted
Domain	Laminin EGF-like domain Repeat Signal
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	neuron remodeling Ref.2 Inferred from direct assay. Source: MGI regulation of branching involved in salivary gland morphogenesis by extracellular matrix-epithelial cell signaling Inferred from direct assay. Source: MGI
Cellular component	basement membrane Inferred from direct assay. Source: MGI
Molecular function	laminin-1 binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 628	609	Netrin-4		PRO_0000042117
Regions
	Domain	30 – 261	232	Laminin N-terminal
	Domain	262 – 331	70	Laminin EGF-like 1
	Domain	332 – 394	63	Laminin EGF-like 2
	Domain	395 – 448	54	Laminin EGF-like 3
	Domain	506 – 627	122	NTR
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Glycosylation	353	1	N-linked (GlcNAc...) Potential
	Glycosylation	483	1	N-linked (GlcNAc...) Potential
	Disulfide bond	262 ↔ 271		By similarity
	Disulfide bond	264 ↔ 293		By similarity
	Disulfide bond	295 ↔ 304		By similarity
	Disulfide bond	307 ↔ 329		By similarity
	Disulfide bond	332 ↔ 341		By similarity
	Disulfide bond	334 ↔ 359		By similarity
	Disulfide bond	362 ↔ 371		By similarity
	Disulfide bond	374 ↔ 392		By similarity
	Disulfide bond	395 ↔ 413		By similarity
	Disulfide bond	397 ↔ 420		By similarity
	Disulfide bond	422 ↔ 431		By similarity
	Disulfide bond	434 ↔ 446		By similarity
	Disulfide bond	506 ↔ 576		By similarity
	Disulfide bond	520 ↔ 627		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JI33-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 474F747475F236C7	FASTA	628	69,897
	10 20 30 40 50 60 MGSCARLLLL WGCSAVAAGL NGVAGANSRC EKACNPRMGN LALGRKLRAD TMCGQNATEL 70 80 90 100 110 120 FCFYSENADL TCRQPKCDKC NAAHSHLAHP PSAMADSSFR FPRTWWQSAE DVHREKIQLD 130 140 150 160 170 180 LEAEFYFTHL IMVFKSPRPA AMVLDRSQDF GKTWKPYKYF ATNCSATFGL EDDVVKKGAI 190 200 210 220 230 240 CTSRYSNPFP CTGGEVIFRA LSPPYDIENP YSAKVQEQLK ITNLRVRLLK RQSCPCQIND 250 260 270 280 290 300 LNAKPHHFMH YAVYDFIVKG SCFCNGHADQ CLPVEGFRPI KAPGAFHVVH GRCMCKHNTA 310 320 330 340 350 360 GSHCQHCAPL YNDRPWEAAD GRTGAPNECR TCKCNGHADT CHFDVNVWEA SGNRSGGVCN 370 380 390 400 410 420 NCQHNTEGQH CQRCKPGFYR DLRRPFSAPD ACKACSCHPV GSAILPFSSV TFCDPSNGDC 430 440 450 460 470 480 PCKPGVAGPH CDRCMVGYWG FGDYGCRPCD CAGSCDPLTG DCISSNADVD WYHEVPTFHS 490 500 510 520 530 540 MHNKSEPSWE WEDEQGFSAL RHSGKCECKE QVLGNPKAFC GMKYSYVLKI KILSAHDKGS 550 560 570 580 590 600 HAEVNVKIKK VLKSTKLKIL RGKRTLYPES WTNRGCTCPI LNPGLEYLVA GHEDVRTGKL 610 620 IVNMKSFVQH WKPALGRRVM HILKRDCV

References

[1]	"Identification and expression of mouse netrin-4." Yin Y., Sanes J.R., Miner J.H. Mech. Dev. 96:115-119(2000) [PubMed: 10940631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: ICR.
[2]	"A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin. Identification, expression, and functional characterization." Koch M., Murrell J.R., Hunter D.D., Olson P.F., Jin W., Keene D.R., Brunken W.J., Burgeson R.E. J. Cell Biol. 151:221-234(2000) [PubMed: 11038171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: BALB/c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF268066 mRNA. Translation: AAF91404.1. AF281278 mRNA. Translation: AAG30823.1.
IPI	IPI00119840.
UniGene	Mm.448571
3D structure databases
HSSP	HSSP built from PDB template 1NPE based on UniProtKB P02468.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9JI33.
Proteomic databases
PRIDE	Q9JI33.
Genome annotation databases
Ensembl	ENSMUST00000020204; ENSMUSP00000020204; ENSMUSG00000020019; Mus musculus. [Genome view]
UCSC	uc007gux.1. mouse.
Organism-specific databases
MGI	MGI:1888978. Ntn4.
Phylogenomic databases
HOGENOM	Q9JI33.
HOVERGEN	Q9JI33.
Gene expression databases
ArrayExpress	Q9JI33.
Bgee	Q9JI33.
CleanEx	MM_NTN4.
Genevestigator	Q9JI33.
GermOnline	ENSMUSG00000020019. Mus musculus.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR002049. EGF_laminin. IPR008211. Laminin_N. IPR001134. Netrin_domain. IPR018933. Netrin_module_non-TIMP. [Graphical view]
Pfam	PF00053. Laminin_EGF. 3 hits. PF00055. Laminin_N. 1 hit. PF01759. NTR. 1 hit. [Graphical view]
PRINTS	PR00011. EGFLAMININ.
SMART	SM00643. C345C. 1 hit. SM00180. EGF_Lam. 3 hits. SM00136. LamNT. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 2 hits. PS01248. EGF_LAM_1. 2 hits. PS50027. EGF_LAM_2. 3 hits. PS51117. LAMININ_NTER. 1 hit. PS50189. NTR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	313932.
SOURCE	Search...

Entry information

Entry name

NET4_MOUSE

Accession

Primary (citable) accession number: Q9JI33

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	October 1, 2000
Last modified:	October 13, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)