Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Netrin-4

Gene

Ntn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play an important role in neural, kidney and vascular development. Promotes neurite elongation from olfactory bulb explants.1 Publication

GO - Molecular functioni

  • laminin-1 binding Source: MGI

GO - Biological processi

  • neuron remodeling Source: MGI
  • regulation of branching involved in salivary gland morphogenesis by extracellular matrix-epithelial cell signaling Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-373752 Netrin-1 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Netrin-4
Alternative name(s):
Beta-netrin
Gene namesi
Name:Ntn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1888978 Ntn4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000004211720 – 628Netrin-4Add BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi56N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi163N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi262 ↔ 271By similarity
Disulfide bondi264 ↔ 293By similarity
Disulfide bondi295 ↔ 304By similarity
Disulfide bondi307 ↔ 329By similarity
Disulfide bondi332 ↔ 341By similarity
Disulfide bondi334 ↔ 359By similarity
Glycosylationi353N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi362 ↔ 371By similarity
Disulfide bondi374 ↔ 392By similarity
Disulfide bondi395 ↔ 413By similarity
Disulfide bondi397 ↔ 420By similarity
Disulfide bondi422 ↔ 431By similarity
Disulfide bondi434 ↔ 446By similarity
Glycosylationi483N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi506 ↔ 576By similarity
Disulfide bondi520 ↔ 627By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9JI33
PaxDbiQ9JI33
PRIDEiQ9JI33

PTM databases

PhosphoSitePlusiQ9JI33

Expressioni

Tissue specificityi

Expressed in kidney, liver, heart, ovary, testis, retina, brain, olfactory bulb, and widely expressed in embryo.2 Publications

Gene expression databases

BgeeiENSMUSG00000020019
CleanExiMM_NTN4
ExpressionAtlasiQ9JI33 baseline and differential
GenevisibleiQ9JI33 MM

Interactioni

Subunit structurei

May form a homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
Lamc1P024682EBI-15755373,EBI-7059830

GO - Molecular functioni

  • laminin-1 binding Source: MGI

Protein-protein interaction databases

BioGridi208320, 3 interactors
DIPiDIP-60741N
IntActiQ9JI33, 4 interactors
STRINGi10090.ENSMUSP00000020204

Structurei

Secondary structure

1628
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni41 – 44Combined sources4
Beta strandi47 – 50Combined sources4
Beta strandi53 – 57Combined sources5
Beta strandi59 – 62Combined sources4
Beta strandi77 – 80Combined sources4
Turni85 – 87Combined sources3
Helixi91 – 93Combined sources3
Beta strandi94 – 96Combined sources3
Beta strandi115 – 136Combined sources22
Beta strandi140 – 149Combined sources10
Beta strandi155 – 162Combined sources8
Helixi164 – 167Combined sources4
Helixi173 – 176Combined sources4
Beta strandi180 – 183Combined sources4
Beta strandi190 – 193Combined sources4
Beta strandi195 – 201Combined sources7
Helixi203 – 206Combined sources4
Helixi213 – 219Combined sources7
Beta strandi220 – 230Combined sources11
Beta strandi252 – 262Combined sources11
Beta strandi299 – 301Combined sources3
Turni321 – 323Combined sources3
Beta strandi341 – 343Combined sources3
Helixi345 – 350Combined sources6
Beta strandi357 – 361Combined sources5
Beta strandi378 – 380Combined sources3
Helixi389 – 391Combined sources3
Beta strandi392 – 394Combined sources3
Turni399 – 401Combined sources3
Turni415 – 417Combined sources3
Beta strandi426 – 430Combined sources5
Beta strandi438 – 442Combined sources5
Beta strandi445 – 448Combined sources4
Turni457 – 459Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WNXX-ray2.72A30-462[»]
ProteinModelPortaliQ9JI33
SMRiQ9JI33
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 261Laminin N-terminalPROSITE-ProRule annotationAdd BLAST232
Domaini262 – 331Laminin EGF-like 1PROSITE-ProRule annotationAdd BLAST70
Domaini332 – 394Laminin EGF-like 2PROSITE-ProRule annotationAdd BLAST63
Domaini395 – 448Laminin EGF-like 3PROSITE-ProRule annotationAdd BLAST54
Domaini506 – 627NTRPROSITE-ProRule annotationAdd BLAST122

Keywords - Domaini

Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0994 Eukaryota
ENOG410XPEG LUCA
GeneTreeiENSGT00780000121851
HOGENOMiHOG000060085
HOVERGENiHBG082019
InParanoidiQ9JI33
KOiK06845
OMAiIVKGSCF
OrthoDBiEOG091G0A35
TreeFamiTF352481

Family and domain databases

CDDicd03578 NTR_netrin-4_like, 1 hit
Gene3Di2.60.120.1490, 1 hit
InterProiView protein in InterPro
IPR002049 Laminin_EGF
IPR008211 Laminin_N
IPR038684 Laminin_N_sf
IPR035811 Netrin-4_NTR
IPR001134 Netrin_domain
IPR018933 Netrin_module_non-TIMP
IPR008993 TIMP-like_OB-fold
PfamiView protein in Pfam
PF00053 Laminin_EGF, 3 hits
PF00055 Laminin_N, 1 hit
PF01759 NTR, 1 hit
SMARTiView protein in SMART
SM00643 C345C, 1 hit
SM00180 EGF_Lam, 3 hits
SM00136 LamNT, 1 hit
SUPFAMiSSF50242 SSF50242, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 2 hits
PS01248 EGF_LAM_1, 2 hits
PS50027 EGF_LAM_2, 3 hits
PS51117 LAMININ_NTER, 1 hit
PS50189 NTR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JI33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSCARLLLL WGCSAVAAGL NGVAGANSRC EKACNPRMGN LALGRKLRAD
60 70 80 90 100
TMCGQNATEL FCFYSENADL TCRQPKCDKC NAAHSHLAHP PSAMADSSFR
110 120 130 140 150
FPRTWWQSAE DVHREKIQLD LEAEFYFTHL IMVFKSPRPA AMVLDRSQDF
160 170 180 190 200
GKTWKPYKYF ATNCSATFGL EDDVVKKGAI CTSRYSNPFP CTGGEVIFRA
210 220 230 240 250
LSPPYDIENP YSAKVQEQLK ITNLRVRLLK RQSCPCQIND LNAKPHHFMH
260 270 280 290 300
YAVYDFIVKG SCFCNGHADQ CLPVEGFRPI KAPGAFHVVH GRCMCKHNTA
310 320 330 340 350
GSHCQHCAPL YNDRPWEAAD GRTGAPNECR TCKCNGHADT CHFDVNVWEA
360 370 380 390 400
SGNRSGGVCN NCQHNTEGQH CQRCKPGFYR DLRRPFSAPD ACKACSCHPV
410 420 430 440 450
GSAILPFSSV TFCDPSNGDC PCKPGVAGPH CDRCMVGYWG FGDYGCRPCD
460 470 480 490 500
CAGSCDPLTG DCISSNADVD WYHEVPAFHS MHNKSEPSWE WEDEQGFSAL
510 520 530 540 550
RHSGKCECKE QVLGNPKAFC GMKYSYVLKI KILSAHDKGS HAEVNVKIKK
560 570 580 590 600
VLKSTKLKIL RGKRTLYPES WTNRGCTCPI LNPGLEYLVA GHEDVRTGKL
610 620
IVNMKSFVQH WKPALGRRVM HILKRDCV
Length:628
Mass (Da):69,867
Last modified:July 27, 2011 - v2
Checksum:i30C5553175E6678D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti477A → T in AAF91404 (PubMed:10940631).Curated1
Sequence conflicti477A → T in AAG30823 (PubMed:11038171).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF268066 mRNA Translation: AAF91404.1
AF281278 mRNA Translation: AAG30823.1
AC124585 Genomic DNA No translation available.
AC151976 Genomic DNA No translation available.
CCDSiCCDS24128.1
RefSeqiNP_067295.2, NM_021320.3
UniGeneiMm.483868

Genome annotation databases

EnsembliENSMUST00000020204; ENSMUSP00000020204; ENSMUSG00000020019
GeneIDi57764
KEGGimmu:57764
UCSCiuc007gux.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNET4_MOUSE
AccessioniPrimary (citable) accession number: Q9JI33
Secondary accession number(s): E9QMT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health