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Protein

Netrin-4

Gene

Ntn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play an important role in neural, kidney and vascular development. Promotes neurite elongation from olfactory bulb explants.1 Publication

GO - Molecular functioni

  • laminin-1 binding Source: MGI

GO - Biological processi

  • neuron remodeling Source: MGI
  • regulation of branching involved in salivary gland morphogenesis by extracellular matrix-epithelial cell signaling Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-373752. Netrin-1 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Netrin-4
Alternative name(s):
Beta-netrin
Gene namesi
Name:Ntn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1888978. Ntn4.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 628609Netrin-4PRO_0000042117Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence analysis
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence analysis
Disulfide bondi262 ↔ 271By similarity
Disulfide bondi264 ↔ 293By similarity
Disulfide bondi295 ↔ 304By similarity
Disulfide bondi307 ↔ 329By similarity
Disulfide bondi332 ↔ 341By similarity
Disulfide bondi334 ↔ 359By similarity
Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence analysis
Disulfide bondi362 ↔ 371By similarity
Disulfide bondi374 ↔ 392By similarity
Disulfide bondi395 ↔ 413By similarity
Disulfide bondi397 ↔ 420By similarity
Disulfide bondi422 ↔ 431By similarity
Disulfide bondi434 ↔ 446By similarity
Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence analysis
Disulfide bondi506 ↔ 576By similarity
Disulfide bondi520 ↔ 627By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9JI33.
PaxDbiQ9JI33.
PRIDEiQ9JI33.

PTM databases

PhosphoSiteiQ9JI33.

Expressioni

Tissue specificityi

Expressed in kidney, liver, heart, ovary, testis, retina, brain, olfactory bulb, and widely expressed in embryo.2 Publications

Gene expression databases

BgeeiQ9JI33.
CleanExiMM_NTN4.
ExpressionAtlasiQ9JI33. baseline and differential.
GenevisibleiQ9JI33. MM.

Interactioni

Subunit structurei

May form a homodimer.

GO - Molecular functioni

  • laminin-1 binding Source: MGI

Protein-protein interaction databases

BioGridi208320. 3 interactions.
DIPiDIP-60741N.
STRINGi10090.ENSMUSP00000020204.

Structurei

Secondary structure

1
628
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni41 – 444Combined sources
Beta strandi47 – 504Combined sources
Beta strandi53 – 575Combined sources
Beta strandi59 – 624Combined sources
Beta strandi77 – 804Combined sources
Turni85 – 873Combined sources
Helixi91 – 933Combined sources
Beta strandi94 – 963Combined sources
Beta strandi115 – 13622Combined sources
Beta strandi140 – 14910Combined sources
Beta strandi155 – 1628Combined sources
Helixi164 – 1674Combined sources
Helixi173 – 1764Combined sources
Beta strandi180 – 1834Combined sources
Beta strandi190 – 1934Combined sources
Beta strandi195 – 2017Combined sources
Helixi203 – 2064Combined sources
Helixi213 – 2197Combined sources
Beta strandi220 – 23011Combined sources
Beta strandi252 – 26211Combined sources
Beta strandi299 – 3013Combined sources
Turni321 – 3233Combined sources
Beta strandi341 – 3433Combined sources
Helixi345 – 3506Combined sources
Beta strandi357 – 3615Combined sources
Beta strandi378 – 3803Combined sources
Helixi389 – 3913Combined sources
Beta strandi392 – 3943Combined sources
Turni399 – 4013Combined sources
Turni415 – 4173Combined sources
Beta strandi426 – 4305Combined sources
Beta strandi438 – 4425Combined sources
Beta strandi445 – 4484Combined sources
Turni457 – 4593Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WNXX-ray2.72A30-462[»]
ProteinModelPortaliQ9JI33.
SMRiQ9JI33. Positions 31-462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 261232Laminin N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini262 – 33170Laminin EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini332 – 39463Laminin EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini395 – 44854Laminin EGF-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini506 – 627122NTRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 laminin EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin N-terminal domain.PROSITE-ProRule annotation
Contains 1 NTR domain.PROSITE-ProRule annotation

Keywords - Domaini

Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0994. Eukaryota.
ENOG410XPEG. LUCA.
GeneTreeiENSGT00780000121851.
HOGENOMiHOG000060085.
HOVERGENiHBG082019.
InParanoidiQ9JI33.
KOiK06845.
OMAiECRTCKC.
OrthoDBiEOG7BKCT6.
TreeFamiTF352481.

Family and domain databases

InterProiIPR002049. Laminin_EGF.
IPR008211. Laminin_N.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01248. EGF_LAM_1. 2 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JI33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSCARLLLL WGCSAVAAGL NGVAGANSRC EKACNPRMGN LALGRKLRAD
60 70 80 90 100
TMCGQNATEL FCFYSENADL TCRQPKCDKC NAAHSHLAHP PSAMADSSFR
110 120 130 140 150
FPRTWWQSAE DVHREKIQLD LEAEFYFTHL IMVFKSPRPA AMVLDRSQDF
160 170 180 190 200
GKTWKPYKYF ATNCSATFGL EDDVVKKGAI CTSRYSNPFP CTGGEVIFRA
210 220 230 240 250
LSPPYDIENP YSAKVQEQLK ITNLRVRLLK RQSCPCQIND LNAKPHHFMH
260 270 280 290 300
YAVYDFIVKG SCFCNGHADQ CLPVEGFRPI KAPGAFHVVH GRCMCKHNTA
310 320 330 340 350
GSHCQHCAPL YNDRPWEAAD GRTGAPNECR TCKCNGHADT CHFDVNVWEA
360 370 380 390 400
SGNRSGGVCN NCQHNTEGQH CQRCKPGFYR DLRRPFSAPD ACKACSCHPV
410 420 430 440 450
GSAILPFSSV TFCDPSNGDC PCKPGVAGPH CDRCMVGYWG FGDYGCRPCD
460 470 480 490 500
CAGSCDPLTG DCISSNADVD WYHEVPAFHS MHNKSEPSWE WEDEQGFSAL
510 520 530 540 550
RHSGKCECKE QVLGNPKAFC GMKYSYVLKI KILSAHDKGS HAEVNVKIKK
560 570 580 590 600
VLKSTKLKIL RGKRTLYPES WTNRGCTCPI LNPGLEYLVA GHEDVRTGKL
610 620
IVNMKSFVQH WKPALGRRVM HILKRDCV
Length:628
Mass (Da):69,867
Last modified:July 27, 2011 - v2
Checksum:i30C5553175E6678D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti477 – 4771A → T in AAF91404 (PubMed:10940631).Curated
Sequence conflicti477 – 4771A → T in AAG30823 (PubMed:11038171).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF268066 mRNA. Translation: AAF91404.1.
AF281278 mRNA. Translation: AAG30823.1.
AC124585 Genomic DNA. No translation available.
AC151976 Genomic DNA. No translation available.
CCDSiCCDS24128.1.
RefSeqiNP_067295.2. NM_021320.3.
UniGeneiMm.483868.

Genome annotation databases

EnsembliENSMUST00000020204; ENSMUSP00000020204; ENSMUSG00000020019.
GeneIDi57764.
KEGGimmu:57764.
UCSCiuc007gux.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF268066 mRNA. Translation: AAF91404.1.
AF281278 mRNA. Translation: AAG30823.1.
AC124585 Genomic DNA. No translation available.
AC151976 Genomic DNA. No translation available.
CCDSiCCDS24128.1.
RefSeqiNP_067295.2. NM_021320.3.
UniGeneiMm.483868.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WNXX-ray2.72A30-462[»]
ProteinModelPortaliQ9JI33.
SMRiQ9JI33. Positions 31-462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208320. 3 interactions.
DIPiDIP-60741N.
STRINGi10090.ENSMUSP00000020204.

PTM databases

PhosphoSiteiQ9JI33.

Proteomic databases

MaxQBiQ9JI33.
PaxDbiQ9JI33.
PRIDEiQ9JI33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020204; ENSMUSP00000020204; ENSMUSG00000020019.
GeneIDi57764.
KEGGimmu:57764.
UCSCiuc007gux.2. mouse.

Organism-specific databases

CTDi59277.
MGIiMGI:1888978. Ntn4.

Phylogenomic databases

eggNOGiKOG0994. Eukaryota.
ENOG410XPEG. LUCA.
GeneTreeiENSGT00780000121851.
HOGENOMiHOG000060085.
HOVERGENiHBG082019.
InParanoidiQ9JI33.
KOiK06845.
OMAiECRTCKC.
OrthoDBiEOG7BKCT6.
TreeFamiTF352481.

Enzyme and pathway databases

ReactomeiR-MMU-373752. Netrin-1 signaling.

Miscellaneous databases

NextBioi313932.
PROiQ9JI33.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JI33.
CleanExiMM_NTN4.
ExpressionAtlasiQ9JI33. baseline and differential.
GenevisibleiQ9JI33. MM.

Family and domain databases

InterProiIPR002049. Laminin_EGF.
IPR008211. Laminin_N.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01248. EGF_LAM_1. 2 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and expression of mouse netrin-4."
    Yin Y., Sanes J.R., Miner J.H.
    Mech. Dev. 96:115-119(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: ICR.
  2. "A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin. Identification, expression, and functional characterization."
    Koch M., Murrell J.R., Hunter D.D., Olson P.F., Jin W., Keene D.R., Brunken W.J., Burgeson R.E.
    J. Cell Biol. 151:221-234(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: BALB/cJ.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiNET4_MOUSE
AccessioniPrimary (citable) accession number: Q9JI33
Secondary accession number(s): E9QMT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.