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Protein

Vesicular glutamate transporter 2

Gene

Slc17a6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas. May also mediate the transport of inorganic phosphate.6 Publications

GO - Molecular functioni

  • L-glutamate transmembrane transporter activity Source: RGD
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

  • brain development Source: RGD
  • hippocampus development Source: RGD
  • L-glutamate transmembrane transport Source: GOC
  • neural retina development Source: RGD
  • neurotransmitter uptake Source: RGD
  • sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Protein family/group databases

TCDBi2.A.1.14.16. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular glutamate transporter 2
Short name:
VGluT2
Alternative name(s):
Differentiation-associated BNPI
Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 6
Gene namesi
Name:Slc17a6
Synonyms:Dnpi, Vglut2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620531. Slc17a6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7171CytoplasmicSequence analysisAdd
BLAST
Transmembranei72 – 9221HelicalSequence analysisAdd
BLAST
Topological domaini93 – 12533VesicularSequence analysisAdd
BLAST
Transmembranei126 – 14621HelicalSequence analysisAdd
BLAST
Topological domaini147 – 1482CytoplasmicSequence analysis
Transmembranei149 – 16921HelicalSequence analysisAdd
BLAST
Topological domaini170 – 1778VesicularSequence analysis
Transmembranei178 – 19821HelicalSequence analysisAdd
BLAST
Topological domaini199 – 21618CytoplasmicSequence analysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence analysisAdd
BLAST
Topological domaini238 – 2447VesicularSequence analysis
Transmembranei245 – 26521HelicalSequence analysisAdd
BLAST
Topological domaini266 – 31045CytoplasmicSequence analysisAdd
BLAST
Transmembranei311 – 33121HelicalSequence analysisAdd
BLAST
Topological domaini332 – 34918VesicularSequence analysisAdd
BLAST
Transmembranei350 – 37021HelicalSequence analysisAdd
BLAST
Topological domaini371 – 38616CytoplasmicSequence analysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence analysisAdd
BLAST
Topological domaini408 – 4092VesicularSequence analysis
Transmembranei410 – 43021HelicalSequence analysisAdd
BLAST
Topological domaini431 – 44313CytoplasmicSequence analysisAdd
BLAST
Transmembranei444 – 46421HelicalSequence analysisAdd
BLAST
Topological domaini465 – 47713VesicularSequence analysisAdd
BLAST
Transmembranei478 – 49821HelicalSequence analysisAdd
BLAST
Topological domaini499 – 58284CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • early endosome Source: RGD
  • excitatory synapse Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • pericellular basket Source: RGD
  • synaptic vesicle Source: RGD
  • synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi128 – 1281H → A: Greatly lowers glutamate transport. 1 Publication
Mutagenesisi184 – 1841R → A, E or K: Greatly lowers glutamate transport. 1 Publication
Mutagenesisi191 – 1911E → A: Greatly lowers glutamate transport. 1 Publication
Mutagenesisi191 – 1911E → D or Q: Lowers glutamate transport. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 582582Vesicular glutamate transporter 2PRO_0000318171Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence analysis
Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence analysis
Glycosylationi470 – 4701N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9JI12.
PRIDEiQ9JI12.

Expressioni

Tissue specificityi

Expressed in brain (at protein level). Expressed in brainstem, deep nuclei, septal nuclei, nuclei of the diagonal band, frontal cortex, hypothalamus, midbrain, parietal cortex and temporal cortex. Also expressed in pineal gland and islets of Langerhans.4 Publications

Interactioni

Protein-protein interaction databases

BioGridi249986. 1 interaction.
STRINGi10116.ENSRNOP00000022383.

Structurei

3D structure databases

ProteinModelPortaliQ9JI12.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2532. Eukaryota.
ENOG410XPWC. LUCA.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ9JI12.
KOiK12302.
PhylomeDBiQ9JI12.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JI12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESVKQRILA PGKEGIKNFA GKSLGQIYRV LEKKQDNRET IELTEDGKPL
60 70 80 90 100
EVPEKKAPLC DCTCFGLPRR YIIAIMSGLG FCISFGIRCN LGVAIVDMVN
110 120 130 140 150
NSTIHRGGKV IKEKAKFNWD PETVGMIHGS FFWGYIITQI PGGYIASRLA
160 170 180 190 200
ANRVFGAAIL LTSTLNMLIP SAARVHYGCV IFVRILQGLV EGVTYPACHG
210 220 230 240 250
IWSKWAPPLE RSRLATTSFC GSYAGAVIAM PLAGILVQYT GWSSVFYVYG
260 270 280 290 300
SFGMVWYMFW LLVSYESPAK HPTITDEERR YIEESIGESA NLLGAMEKFK
310 320 330 340 350
TPWRKFFTSM PVYAIIVANF CRSWTFYLLL ISQPAYFEEV FGFEISKVGM
360 370 380 390 400
LSAVPHLVMT IIVPIGGQIA DFLRSKQILS TTTVRKIMNC GGFGMEATLL
410 420 430 440 450
LVVGYSHTRG VAISFLVLAV GFSGFAISGF NVNHLDIAPR YASILMGISN
460 470 480 490 500
GVGTLSGMVC PIIVGAMTKN KSREEWQYVF LIAALVHYGG VIFYALFASG
510 520 530 540 550
EKQPWADPEE TSEEKCGFIH EDELDEETGD ITQNYINYGT TKSYGATSQE
560 570 580
NGGWPNGWEK KEEFVQESAQ DAYSYKDRDD YS
Length:582
Mass (Da):64,576
Last modified:October 1, 2000 - v1
Checksum:i99A14F62E685B9E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF271235 mRNA. Translation: AAF76223.1.
RefSeqiNP_445879.1. NM_053427.1.
UniGeneiRn.19372.

Genome annotation databases

GeneIDi84487.
KEGGirno:84487.
UCSCiRGD:620531. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF271235 mRNA. Translation: AAF76223.1.
RefSeqiNP_445879.1. NM_053427.1.
UniGeneiRn.19372.

3D structure databases

ProteinModelPortaliQ9JI12.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249986. 1 interaction.
STRINGi10116.ENSRNOP00000022383.

Protein family/group databases

TCDBi2.A.1.14.16. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiQ9JI12.
PRIDEiQ9JI12.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84487.
KEGGirno:84487.
UCSCiRGD:620531. rat.

Organism-specific databases

CTDi57084.
RGDi620531. Slc17a6.

Phylogenomic databases

eggNOGiKOG2532. Eukaryota.
ENOG410XPWC. LUCA.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ9JI12.
KOiK12302.
PhylomeDBiQ9JI12.

Miscellaneous databases

PROiQ9JI12.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of a novel brain-type Na(+)-dependent inorganic phosphate cotransporter."
    Aihara Y., Mashima H., Onda H., Hisano S., Kasuya H., Hori T., Yamada S., Tomura H., Yamada Y., Inoue I., Kojima I., Takeda J.
    J. Neurochem. 74:2622-2625(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Differentiation-associated Na+-dependent inorganic phosphate cotransporter (DNPI) is a vesicular glutamate transporter in endocrine glutamatergic systems."
    Hayashi M., Otsuka M., Morimoto R., Hirota S., Yatsushiro S., Takeda J., Yamamoto A., Moriyama Y.
    J. Biol. Chem. 276:43400-43406(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons."
    Herzog E., Bellenchi G.C., Gras C., Bernard V., Ravassard P., Bedet C., Gasnier B., Giros B., El Mestikawy S.
    J. Neurosci. 21:RC181-RC181(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. "Identification of differentiation-associated brain-specific phosphate transporter as a second vesicular glutamate transporter (VGLUT2)."
    Takamori S., Rhee J.S., Rosenmund C., Jahn R.
    J. Neurosci. 21:RC182-RC182(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "The expression of vesicular glutamate transporters defines two classes of excitatory synapse."
    Fremeau R.T. Jr., Troyer M.D., Pahner I., Nygaard G.O., Tran C.H., Reimer R.J., Bellocchio E.E., Fortin D., Storm-Mathisen J., Edwards R.H.
    Neuron 31:247-260(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Vesicular glutamate transporter contains two independent transport machineries."
    Juge N., Yoshida Y., Yatsushiro S., Omote H., Moriyama Y.
    J. Biol. Chem. 281:39499-39506(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF HIS-128; ARG-184 AND GLU-191.
  8. "Analysis of a vesicular glutamate transporter (VGLUT2) supports a cell-leakage mode in addition to vesicular packaging."
    Mackenzie B., Illing A.C., Morris M.E.K., Varoqui H., Erickson J.D.
    Neurochem. Res. 33:238-247(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiVGLU2_RAT
AccessioniPrimary (citable) accession number: Q9JI12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.