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Q9JHY8

- DNLI1_RAT

UniProt

Q9JHY8 - DNLI1_RAT

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Protein

DNA ligase 1

Gene

Lig1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Magnesium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei305 – 3051Interaction with target DNABy similarity
Binding sitei566 – 5661ATPBy similarity
Active sitei568 – 5681N6-AMP-lysine intermediateBy similarity
Binding sitei573 – 5731ATPBy similarity
Binding sitei589 – 5891ATPBy similarity
Sitei590 – 5901Interaction with target DNABy similarity
Metal bindingi621 – 6211Magnesium 1By similarity
Metal bindingi720 – 7201Magnesium 2By similarity
Binding sitei725 – 7251ATPBy similarity
Binding sitei738 – 7381ATPBy similarity
Binding sitei744 – 7441ATPBy similarity
Sitei770 – 7701Interaction with target DNABy similarity
Sitei795 – 7951Interaction with target DNABy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: RefGenome
  4. DNA ligase activity Source: RGD
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-KW
  6. DNA repair Source: RGD
  7. lagging strand elongation Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1 (EC:6.5.1.1)
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene namesi
Name:Lig1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621424. Lig1.

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: RefGenome
  2. mitochondrion Source: RefGenome
  3. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918DNA ligase 1PRO_0000059572Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineBy similarity
Modified residuei52 – 521PhosphoserineBy similarity
Modified residuei66 – 661PhosphoserineBy similarity
Modified residuei67 – 671PhosphoserineBy similarity
Modified residuei78 – 781PhosphothreonineBy similarity
Modified residuei145 – 1451N6-acetyllysineBy similarity
Modified residuei195 – 1951PhosphothreonineBy similarity
Modified residuei227 – 2271N6-acetyllysineBy similarity
Modified residuei234 – 2341PhosphothreonineBy similarity
Cross-linki422 – 422Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei913 – 9131PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9JHY8.
PRIDEiQ9JHY8.

PTM databases

PhosphoSiteiQ9JHY8.

Expressioni

Gene expression databases

GenevestigatoriQ9JHY8.

Interactioni

Protein-protein interaction databases

BioGridi249510. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9JHY8.
SMRiQ9JHY8. Positions 262-901.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni642 – 6443Interaction with target DNABy similarity

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036006.
HOVERGENiHBG005514.
InParanoidiQ9JHY8.
PhylomeDBiQ9JHY8.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JHY8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQRSIMSFFQ PTTTEGKAKK PEKEIPSSIR EKEPPPKVAL KERNRAVPES
60 70 80 90 100
DSPVKRPGRK VAQVLSSEGE DEDEAPGTPQ VQKPVSDSKQ SSPPSPDSCP
110 120 130 140 150
ENSPVFNCSP SMDISPSGFP KRRTARKQLP KRTIQDTLEE PNEDKAKAVK
160 170 180 190 200
KRKKEDPQTP PESLTEAEEV NQKEEQVEDQ PTVPPEPTES PESVTLTKTE
210 220 230 240 250
NIPMCKAGVK QKPQEEEQSK PPARGAKPLS SFFTPRKPAV KTEVKQEESD
260 270 280 290 300
TPRKEETKGA PDPTNYNPSK SNYHPIEDAC WKHGQKVPFL AVARTFEKIE
310 320 330 340 350
EVSARLKMVE TLSNLLRSVV ALSPTDLLPV LYLSLNRLGP PQQGLELGVG
360 370 380 390 400
DGVLLKAVAQ ATGRQLESIR AEVAEKGDVG LVAENSRSTQ RLMLPSPPLT
410 420 430 440 450
VSGVFTKFCD IARLTGSASM AKKMDIIKGL FVACRYSEAR FIARSLSGRL
460 470 480 490 500
RLGLAEQSVL AALAQAGSLT PPGQEFPTVV VDAGKGKTAE ARKMWLEEQG
510 520 530 540 550
MILKQTFCEV PDLDRIIPVL LEHGLESLPE HCKLSPGVPL KPMLAHPTRG
560 570 580 590 600
VREVLKRFEE VDFTCEYKYY GQRAQIHVLE GGEVKIFSRN QEDNSGKYPD
610 620 630 640 650
IISRIPKIKH PSVTSFILDT EAVAWDREKK QIQPFQVLTT RKRKEVDASE
660 670 680 690 700
IQVQVCLYAF DLIYLNGESL ARQPLSRRRQ LLRENFVETE GEFVFATSLD
710 720 730 740 750
TKDIEQIAEF LEQSVKDSCE GLMVKTLDVD ATYEIAKRSH NWLKLKKDYL
760 770 780 790 800
EGVGDTLDLV VIGAYLGRGK RPGRYGGFLL AAYDEESEEL AAICKLGTGF
810 820 830 840 850
SDEELEEHHQ NMQALLLPTP RPYVRIDGAV APNHWLDPSI VWEVKCADLT
860 870 880 890 900
LSPIYRAARG MVDKEKGISL RFPRFIRVRE DKQPEQATTS DQVASLYRKQ
910
SQIQNQQSSD LDSDVEDY
Length:918
Mass (Da):102,482
Last modified:October 1, 2000 - v1
Checksum:iB4ECA8861C521567
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF276774 mRNA. Translation: AAF82585.1.
UniGeneiRn.20467.

Genome annotation databases

UCSCiRGD:621424. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF276774 mRNA. Translation: AAF82585.1 .
UniGenei Rn.20467.

3D structure databases

ProteinModelPortali Q9JHY8.
SMRi Q9JHY8. Positions 262-901.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249510. 1 interaction.

PTM databases

PhosphoSitei Q9JHY8.

Proteomic databases

PaxDbi Q9JHY8.
PRIDEi Q9JHY8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:621424. rat.

Organism-specific databases

RGDi 621424. Lig1.

Phylogenomic databases

eggNOGi COG1793.
HOGENOMi HOG000036006.
HOVERGENi HBG005514.
InParanoidi Q9JHY8.
PhylomeDBi Q9JHY8.

Miscellaneous databases

NextBioi 614997.
PROi Q9JHY8.

Gene expression databases

Genevestigatori Q9JHY8.

Family and domain databases

Gene3Di 1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProi IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of rat DNA ligase I."
    Chen D., Cao G., Chen J.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiDNLI1_RAT
AccessioniPrimary (citable) accession number: Q9JHY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3