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Q9JHY8

- DNLI1_RAT

UniProt

Q9JHY8 - DNLI1_RAT

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Protein
DNA ligase 1
Gene
Lig1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei305 – 3051Interaction with target DNA By similarity
Binding sitei566 – 5661ATP By similarity
Active sitei568 – 5681N6-AMP-lysine intermediate By similarity
Binding sitei573 – 5731ATP By similarity
Binding sitei589 – 5891ATP By similarity
Sitei590 – 5901Interaction with target DNA By similarity
Metal bindingi621 – 6211Magnesium 1 By similarity
Metal bindingi720 – 7201Magnesium 2 By similarity
Binding sitei725 – 7251ATP By similarity
Binding sitei738 – 7381ATP By similarity
Binding sitei744 – 7441ATP By similarity
Sitei770 – 7701Interaction with target DNA By similarity
Sitei795 – 7951Interaction with target DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: RefGenome
  4. DNA ligase activity Source: RGD
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA ligation involved in DNA repair Source: InterPro
  2. DNA recombination Source: UniProtKB-KW
  3. DNA repair Source: RGD
  4. cell cycle Source: UniProtKB-KW
  5. cell division Source: UniProtKB-KW
  6. lagging strand elongation Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1 (EC:6.5.1.1)
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene namesi
Name:Lig1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621424. Lig1.

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: RefGenome
  2. mitochondrion Source: RefGenome
  3. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918DNA ligase 1
PRO_0000059572Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501Phosphoserine By similarity
Modified residuei52 – 521Phosphoserine By similarity
Modified residuei66 – 661Phosphoserine By similarity
Modified residuei67 – 671Phosphoserine By similarity
Modified residuei78 – 781Phosphothreonine By similarity
Modified residuei145 – 1451N6-acetyllysine By similarity
Modified residuei195 – 1951Phosphothreonine By similarity
Modified residuei227 – 2271N6-acetyllysine By similarity
Modified residuei234 – 2341Phosphothreonine By similarity
Cross-linki422 – 422Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Modified residuei913 – 9131Phosphoserine By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9JHY8.
PRIDEiQ9JHY8.

PTM databases

PhosphoSiteiQ9JHY8.

Expressioni

Gene expression databases

GenevestigatoriQ9JHY8.

Interactioni

Protein-protein interaction databases

BioGridi249510. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9JHY8.
SMRiQ9JHY8. Positions 262-901.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni642 – 6443Interaction with target DNA By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036006.
HOVERGENiHBG005514.
InParanoidiQ9JHY8.
PhylomeDBiQ9JHY8.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JHY8-1 [UniParc]FASTAAdd to Basket

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MQRSIMSFFQ PTTTEGKAKK PEKEIPSSIR EKEPPPKVAL KERNRAVPES    50
DSPVKRPGRK VAQVLSSEGE DEDEAPGTPQ VQKPVSDSKQ SSPPSPDSCP 100
ENSPVFNCSP SMDISPSGFP KRRTARKQLP KRTIQDTLEE PNEDKAKAVK 150
KRKKEDPQTP PESLTEAEEV NQKEEQVEDQ PTVPPEPTES PESVTLTKTE 200
NIPMCKAGVK QKPQEEEQSK PPARGAKPLS SFFTPRKPAV KTEVKQEESD 250
TPRKEETKGA PDPTNYNPSK SNYHPIEDAC WKHGQKVPFL AVARTFEKIE 300
EVSARLKMVE TLSNLLRSVV ALSPTDLLPV LYLSLNRLGP PQQGLELGVG 350
DGVLLKAVAQ ATGRQLESIR AEVAEKGDVG LVAENSRSTQ RLMLPSPPLT 400
VSGVFTKFCD IARLTGSASM AKKMDIIKGL FVACRYSEAR FIARSLSGRL 450
RLGLAEQSVL AALAQAGSLT PPGQEFPTVV VDAGKGKTAE ARKMWLEEQG 500
MILKQTFCEV PDLDRIIPVL LEHGLESLPE HCKLSPGVPL KPMLAHPTRG 550
VREVLKRFEE VDFTCEYKYY GQRAQIHVLE GGEVKIFSRN QEDNSGKYPD 600
IISRIPKIKH PSVTSFILDT EAVAWDREKK QIQPFQVLTT RKRKEVDASE 650
IQVQVCLYAF DLIYLNGESL ARQPLSRRRQ LLRENFVETE GEFVFATSLD 700
TKDIEQIAEF LEQSVKDSCE GLMVKTLDVD ATYEIAKRSH NWLKLKKDYL 750
EGVGDTLDLV VIGAYLGRGK RPGRYGGFLL AAYDEESEEL AAICKLGTGF 800
SDEELEEHHQ NMQALLLPTP RPYVRIDGAV APNHWLDPSI VWEVKCADLT 850
LSPIYRAARG MVDKEKGISL RFPRFIRVRE DKQPEQATTS DQVASLYRKQ 900
SQIQNQQSSD LDSDVEDY 918
Length:918
Mass (Da):102,482
Last modified:October 1, 2000 - v1
Checksum:iB4ECA8861C521567
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF276774 mRNA. Translation: AAF82585.1.
UniGeneiRn.20467.

Genome annotation databases

UCSCiRGD:621424. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF276774 mRNA. Translation: AAF82585.1 .
UniGenei Rn.20467.

3D structure databases

ProteinModelPortali Q9JHY8.
SMRi Q9JHY8. Positions 262-901.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249510. 1 interaction.

PTM databases

PhosphoSitei Q9JHY8.

Proteomic databases

PaxDbi Q9JHY8.
PRIDEi Q9JHY8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:621424. rat.

Organism-specific databases

RGDi 621424. Lig1.

Phylogenomic databases

eggNOGi COG1793.
HOGENOMi HOG000036006.
HOVERGENi HBG005514.
InParanoidi Q9JHY8.
PhylomeDBi Q9JHY8.

Miscellaneous databases

NextBioi 614997.
PROi Q9JHY8.

Gene expression databases

Genevestigatori Q9JHY8.

Family and domain databases

Gene3Di 1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProi IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of rat DNA ligase I."
    Chen D., Cao G., Chen J.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiDNLI1_RAT
AccessioniPrimary (citable) accession number: Q9JHY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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