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Protein

DNA ligase 1

Gene

Lig1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei566ATPBy similarity1
Active sitei568N6-AMP-lysine intermediateBy similarity1
Binding sitei573ATPBy similarity1
Binding sitei589ATPBy similarity1
Metal bindingi621Magnesium 1By similarity1
Metal bindingi720Magnesium 2By similarity1
Binding sitei725ATPBy similarity1
Binding sitei738ATPBy similarity1
Binding sitei744ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: InterPro
  • DNA ligase (ATP) activity Source: GO_Central
  • DNA ligase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1 (EC:6.5.1.1)
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene namesi
Name:Lig1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621424. Lig1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: GO_Central
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000595721 – 918DNA ligase 1Add BLAST918

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineBy similarity1
Modified residuei52PhosphoserineBy similarity1
Modified residuei66PhosphoserineCombined sources1
Modified residuei67PhosphoserineCombined sources1
Modified residuei78PhosphothreonineCombined sources1
Modified residuei145N6-acetyllysineBy similarity1
Modified residuei195PhosphothreonineBy similarity1
Modified residuei227N6-acetyllysineBy similarity1
Modified residuei230PhosphoserineBy similarity1
Modified residuei231PhosphoserineBy similarity1
Modified residuei234PhosphothreonineBy similarity1
Modified residuei798PhosphothreonineBy similarity1
Modified residuei801PhosphoserineBy similarity1
Modified residuei908PhosphoserineBy similarity1
Modified residuei909PhosphoserineBy similarity1
Modified residuei913PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9JHY8.
PRIDEiQ9JHY8.

PTM databases

iPTMnetiQ9JHY8.
PhosphoSitePlusiQ9JHY8.

Interactioni

Subunit structurei

Interacts with PCNA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei305Interaction with target DNABy similarity1
Sitei590Interaction with target DNABy similarity1
Sitei770Interaction with target DNABy similarity1
Sitei795Interaction with target DNABy similarity1

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019799.

Structurei

3D structure databases

ProteinModelPortaliQ9JHY8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni642 – 644Interaction with target DNABy similarity3

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Phylogenomic databases

eggNOGiKOG0967. Eukaryota.
COG1793. LUCA.
HOGENOMiHOG000036006.
HOVERGENiHBG005514.
InParanoidiQ9JHY8.
PhylomeDBiQ9JHY8.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JHY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRSIMSFFQ PTTTEGKAKK PEKEIPSSIR EKEPPPKVAL KERNRAVPES
60 70 80 90 100
DSPVKRPGRK VAQVLSSEGE DEDEAPGTPQ VQKPVSDSKQ SSPPSPDSCP
110 120 130 140 150
ENSPVFNCSP SMDISPSGFP KRRTARKQLP KRTIQDTLEE PNEDKAKAVK
160 170 180 190 200
KRKKEDPQTP PESLTEAEEV NQKEEQVEDQ PTVPPEPTES PESVTLTKTE
210 220 230 240 250
NIPMCKAGVK QKPQEEEQSK PPARGAKPLS SFFTPRKPAV KTEVKQEESD
260 270 280 290 300
TPRKEETKGA PDPTNYNPSK SNYHPIEDAC WKHGQKVPFL AVARTFEKIE
310 320 330 340 350
EVSARLKMVE TLSNLLRSVV ALSPTDLLPV LYLSLNRLGP PQQGLELGVG
360 370 380 390 400
DGVLLKAVAQ ATGRQLESIR AEVAEKGDVG LVAENSRSTQ RLMLPSPPLT
410 420 430 440 450
VSGVFTKFCD IARLTGSASM AKKMDIIKGL FVACRYSEAR FIARSLSGRL
460 470 480 490 500
RLGLAEQSVL AALAQAGSLT PPGQEFPTVV VDAGKGKTAE ARKMWLEEQG
510 520 530 540 550
MILKQTFCEV PDLDRIIPVL LEHGLESLPE HCKLSPGVPL KPMLAHPTRG
560 570 580 590 600
VREVLKRFEE VDFTCEYKYY GQRAQIHVLE GGEVKIFSRN QEDNSGKYPD
610 620 630 640 650
IISRIPKIKH PSVTSFILDT EAVAWDREKK QIQPFQVLTT RKRKEVDASE
660 670 680 690 700
IQVQVCLYAF DLIYLNGESL ARQPLSRRRQ LLRENFVETE GEFVFATSLD
710 720 730 740 750
TKDIEQIAEF LEQSVKDSCE GLMVKTLDVD ATYEIAKRSH NWLKLKKDYL
760 770 780 790 800
EGVGDTLDLV VIGAYLGRGK RPGRYGGFLL AAYDEESEEL AAICKLGTGF
810 820 830 840 850
SDEELEEHHQ NMQALLLPTP RPYVRIDGAV APNHWLDPSI VWEVKCADLT
860 870 880 890 900
LSPIYRAARG MVDKEKGISL RFPRFIRVRE DKQPEQATTS DQVASLYRKQ
910
SQIQNQQSSD LDSDVEDY
Length:918
Mass (Da):102,482
Last modified:October 1, 2000 - v1
Checksum:iB4ECA8861C521567
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276774 mRNA. Translation: AAF82585.1.
UniGeneiRn.20467.

Genome annotation databases

UCSCiRGD:621424. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276774 mRNA. Translation: AAF82585.1.
UniGeneiRn.20467.

3D structure databases

ProteinModelPortaliQ9JHY8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019799.

PTM databases

iPTMnetiQ9JHY8.
PhosphoSitePlusiQ9JHY8.

Proteomic databases

PaxDbiQ9JHY8.
PRIDEiQ9JHY8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621424. rat.

Organism-specific databases

RGDi621424. Lig1.

Phylogenomic databases

eggNOGiKOG0967. Eukaryota.
COG1793. LUCA.
HOGENOMiHOG000036006.
HOVERGENiHBG005514.
InParanoidiQ9JHY8.
PhylomeDBiQ9JHY8.

Miscellaneous databases

PROiQ9JHY8.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI1_RAT
AccessioniPrimary (citable) accession number: Q9JHY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.