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Q9JHY8 (DNLI1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase 1
EC=6.5.1.1
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene names
Name:Lig1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length918 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 918918DNA ligase 1
PRO_0000059572

Regions

Region642 – 6443Interaction with target DNA By similarity

Sites

Active site5681N6-AMP-lysine intermediate By similarity
Metal binding6211Magnesium 1 By similarity
Metal binding7201Magnesium 2 By similarity
Binding site5661ATP By similarity
Binding site5731ATP By similarity
Binding site5891ATP By similarity
Binding site7251ATP By similarity
Binding site7381ATP By similarity
Binding site7441ATP By similarity
Site3051Interaction with target DNA By similarity
Site5901Interaction with target DNA By similarity
Site7701Interaction with target DNA By similarity
Site7951Interaction with target DNA By similarity

Amino acid modifications

Modified residue501Phosphoserine By similarity
Modified residue521Phosphoserine By similarity
Modified residue671Phosphoserine By similarity
Modified residue781Phosphothreonine By similarity
Modified residue1901Phosphoserine By similarity
Modified residue1951Phosphothreonine By similarity
Modified residue2341Phosphothreonine By similarity
Modified residue9131Phosphoserine By similarity
Cross-link422Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9JHY8 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: B4ECA8861C521567

FASTA918102,482
        10         20         30         40         50         60 
MQRSIMSFFQ PTTTEGKAKK PEKEIPSSIR EKEPPPKVAL KERNRAVPES DSPVKRPGRK 

        70         80         90        100        110        120 
VAQVLSSEGE DEDEAPGTPQ VQKPVSDSKQ SSPPSPDSCP ENSPVFNCSP SMDISPSGFP 

       130        140        150        160        170        180 
KRRTARKQLP KRTIQDTLEE PNEDKAKAVK KRKKEDPQTP PESLTEAEEV NQKEEQVEDQ 

       190        200        210        220        230        240 
PTVPPEPTES PESVTLTKTE NIPMCKAGVK QKPQEEEQSK PPARGAKPLS SFFTPRKPAV 

       250        260        270        280        290        300 
KTEVKQEESD TPRKEETKGA PDPTNYNPSK SNYHPIEDAC WKHGQKVPFL AVARTFEKIE 

       310        320        330        340        350        360 
EVSARLKMVE TLSNLLRSVV ALSPTDLLPV LYLSLNRLGP PQQGLELGVG DGVLLKAVAQ 

       370        380        390        400        410        420 
ATGRQLESIR AEVAEKGDVG LVAENSRSTQ RLMLPSPPLT VSGVFTKFCD IARLTGSASM 

       430        440        450        460        470        480 
AKKMDIIKGL FVACRYSEAR FIARSLSGRL RLGLAEQSVL AALAQAGSLT PPGQEFPTVV 

       490        500        510        520        530        540 
VDAGKGKTAE ARKMWLEEQG MILKQTFCEV PDLDRIIPVL LEHGLESLPE HCKLSPGVPL 

       550        560        570        580        590        600 
KPMLAHPTRG VREVLKRFEE VDFTCEYKYY GQRAQIHVLE GGEVKIFSRN QEDNSGKYPD 

       610        620        630        640        650        660 
IISRIPKIKH PSVTSFILDT EAVAWDREKK QIQPFQVLTT RKRKEVDASE IQVQVCLYAF 

       670        680        690        700        710        720 
DLIYLNGESL ARQPLSRRRQ LLRENFVETE GEFVFATSLD TKDIEQIAEF LEQSVKDSCE 

       730        740        750        760        770        780 
GLMVKTLDVD ATYEIAKRSH NWLKLKKDYL EGVGDTLDLV VIGAYLGRGK RPGRYGGFLL 

       790        800        810        820        830        840 
AAYDEESEEL AAICKLGTGF SDEELEEHHQ NMQALLLPTP RPYVRIDGAV APNHWLDPSI 

       850        860        870        880        890        900 
VWEVKCADLT LSPIYRAARG MVDKEKGISL RFPRFIRVRE DKQPEQATTS DQVASLYRKQ 

       910 
SQIQNQQSSD LDSDVEDY

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References

[1]"Molecular cloning and characterization of rat DNA ligase I."
Chen D., Cao G., Chen J.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF276774 mRNA. Translation: AAF82585.1.
IPIIPI00200072.
UniGeneRn.20467.

3D structure databases

ProteinModelPortalQ9JHY8.
SMRQ9JHY8. Positions 262-901.
ModBaseSearch...

PTM databases

PhosphoSiteQ9JHY8.

Proteomic databases

PaxDbQ9JHY8.
PRIDEQ9JHY8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:621424. rat.

Organism-specific databases

RGD621424. Lig1.

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036006.
HOVERGENHBG005514.
InParanoidQ9JHY8.
OrthoDBEOG4RBQJ6.

Gene expression databases

ArrayExpressQ9JHY8.
GenevestigatorQ9JHY8.
GermOnlineENSRNOG00000014193. Rattus norvegicus.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. False negative.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio614997.

Entry information

Entry nameDNLI1_RAT
AccessionPrimary (citable) accession number: Q9JHY8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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