Q9JHY1 (JAM1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Junctional adhesion molecule A
Short name=JAM-A

Alternative name(s):
Junctional adhesion molecule 1
Short name=JAM-1
CD_antigen=CD321

Gene names

Name:	F11r
Synonyms:	Jam1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus By similarity.
Subunit structure	Interacts with the ninth PDZ domain of MPDZ. Interacts with the first PDZ domain of PARD3. The association between PARD3 and PARD6B probably disrupts this interaction. Interacts with the orthoreovirus sigma-1 capsid protein By similarity.
Subcellular location	Cell junction › tight junction By similarity. Cell membrane; Single-pass type I membrane protein By similarity. Note: Localized at tight junctions of both epithelial and endothelial cells By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the immunoglobulin superfamily. Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Host-virus interaction
Cellular component	Cell junction Cell membrane Membrane Tight junction
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: Compara epithelial cell differentiation Inferred from electronic annotation. Source: Compara virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell tight junction Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 300	274	Junctional adhesion molecule A		PRO_0000015068
Regions
	Topological domain	27 – 238	212	Extracellular Potential
	Transmembrane	239 – 259	21	Helical; Potential
	Topological domain	260 – 300	41	Cytoplasmic Potential
	Domain	28 – 122	95	Ig-like V-type 1
	Domain	134 – 228	95	Ig-like V-type 2
Amino acid modifications
	Modified residue	282	1	Phosphoserine By similarity
	Modified residue	285	1	Phosphoserine Ref.3
	Modified residue	288	1	Phosphoserine By similarity
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
	Disulfide bond	49 ↔ 108		By similarity
	Disulfide bond	152 ↔ 212		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JHY1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 45AE362A96158BFA	FASTA	300	32,370
	10 20 30 40 50 60 MGTEGKAGSK LLFLFTSMIL GSLVQGKGSV YSPQTAVQVP ENDSVKLPCI YSGFSSPRVE 70 80 90 100 110 120 WKFVQGSTTA LVCYNNQITV PYADRVTFSS SGITFSSVTR KDNGEYTCMV SEDGGQNYGE 130 140 150 160 170 180 VSIHLTVLVP PSKPTVSIPS SVTIGNRAVL TCSEHDGSPP SEYSWFKDGV PMLTADAKKT 190 200 210 220 230 240 RAFINSSYTI DPKSGDLVFD PVSAFDSGEY YCEAQNGYGT AMRSEAVRME AVELNVGGIV 250 260 270 280 290 300 AAVLVTLILL GLLIFGIWFA YSRGYFERTK KGTAPGKKVI YSQPSARSEG EFKQTSSFLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mashima H., Kojima I. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[3]	"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS." Moser K., White F.M. J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-285, MASS SPECTROMETRY. Strain: Fischer. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF276998 mRNA. Translation: AAF78250.1. BC065309 mRNA. Translation: AAH65309.1.
IPI	IPI00200067.
RefSeq	NP_446248.1. NM_053796.1.
UniGene	Rn.107273.
3D structure databases
ProteinModelPortal	Q9JHY1.
SMR	Q9JHY1. Positions 27-238.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-135629.
STRING	10116.ENSRNOP00000006141.
PTM databases
PhosphoSite	Q9JHY1.
Proteomic databases
PaxDb	Q9JHY1.
PRIDE	Q9JHY1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000006141; ENSRNOP00000006141; ENSRNOG00000004414.
GeneID	116479.
KEGG	rno:116479.
UCSC	RGD:621842. rat.
Organism-specific databases
CTD	50848.
RGD	621842. F11r.
Phylogenomic databases
eggNOG	NOG150281.
GeneTree	ENSGT00670000097525.
HOGENOM	HOG000247041.
HOVERGEN	HBG000518.
InParanoid	Q9JHY1.
KO	K06089.
OMA	SSPRVEW.
OrthoDB	EOG4VT5XV.
Gene expression databases
ArrayExpress	Q9JHY1.
Genevestigator	Q9JHY1.
GermOnline	ENSRNOG00000004414. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003598. Ig_sub2. IPR013106. Ig_V-set. IPR003596. Ig_V-set_subgr. [Graphical view]
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00408. IGc2. 1 hit. SM00406. IGv. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	619047.

Entry information

Entry name

JAM1_RAT

Accession

Primary (citable) accession number: Q9JHY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program