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Q9JHW9 (AL1A3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aldehyde dehydrogenase family 1 member A3

EC=1.2.1.5
Alternative name(s):
Aldehyde dehydrogenase 6
Retinaldehyde dehydrogenase 3
Short name=RALDH-3
Short name=RalDH3
Gene names
Name:Aldh1a3
Synonyms:Aldh6, Raldh3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system. Ref.1 Ref.2 Ref.3 Ref.4

Catalytic activity

An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.

Pathway

Cofactor metabolism; retinol metabolism.

Subcellular location

Cytoplasm Ref.4.

Tissue specificity

Ventral retina. Ref.1 Ref.2 Ref.3 Ref.4

Developmental stage

In mouse embryos, RALDH3 expression is first noticed in the ventral optic eminence at E8.75, then in the optic vesicle/cup, otic vesicle and olfactory placode/pit from E9.5 to E11.5. Ref.2

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processembryonic camera-type eye development

Inferred from genetic interaction PubMed 17184764. Source: MGI

embryonic eye morphogenesis

Inferred from mutant phenotype PubMed 16207763PubMed 16611695. Source: MGI

face development

Inferred from genetic interaction PubMed 17184764. Source: MGI

inner ear morphogenesis

Inferred from mutant phenotype PubMed 23536097. Source: MGI

locomotory behavior

Inferred from mutant phenotype PubMed 23536097. Source: MGI

neuromuscular process controlling balance

Inferred from mutant phenotype PubMed 23536097. Source: MGI

nucleus accumbens development

Inferred from mutant phenotype PubMed 17207476. Source: MGI

olfactory pit development

Inferred from mutant phenotype PubMed 17207476. Source: MGI

optic cup morphogenesis involved in camera-type eye development

Inferred from genetic interaction PubMed 16611695. Source: MGI

positive regulation of apoptotic process

Inferred from mutant phenotype PubMed 16207763. Source: MGI

positive regulation of retinoic acid receptor signaling pathway

Inferred from sequence alignment Ref.4. Source: MGI

retinal metabolic process

Inferred from electronic annotation. Source: Ensembl

retinoic acid biosynthetic process

Inferred from direct assay Ref.4Ref.3PubMed 16241904. Source: MGI

retinoic acid metabolic process

Inferred from mutant phenotype PubMed 16207763PubMed 17207476. Source: MGI

retinol metabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

righting reflex

Inferred from mutant phenotype PubMed 23536097. Source: MGI

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 16615129. Source: MGI

   Molecular_function3-chloroallyl aldehyde dehydrogenase activity

Traceable author statement Ref.1. Source: MGI

NAD+ binding

Inferred from direct assay PubMed 16241904. Source: MGI

aldehyde dehydrogenase (NAD) activity

Inferred from direct assay Ref.4PubMed 16241904. Source: MGI

aldehyde dehydrogenase [NAD(P)+] activity

Inferred from direct assay Ref.3. Source: MGI

thyroid hormone binding

Inferred from physical interaction PubMed 16241904. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 512511Aldehyde dehydrogenase family 1 member A3
PRO_0000056479

Regions

Nucleotide binding257 – 2626NAD By similarity

Sites

Active site2801Proton acceptor By similarity
Active site3141Nucleophile By similarity
Site1811Transition state stabilizer By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Experimental info

Sequence conflict911L → V in AAF67736. Ref.1
Sequence conflict2081Q → R in AAG33935. Ref.4
Sequence conflict2231V → E in AAF67736. Ref.1
Sequence conflict3411R → S in AAG33935. Ref.4
Sequence conflict4071I → R in AAF67736. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9JHW9 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 5BBC6DEE41E58CFE

FASTA51256,157
        10         20         30         40         50         60 
MATTNGAVEN GQPDGKPPAL PRPIRNLEVK FTKIFINNDW HESKSGRKFA TYNPSTLEKI 

        70         80         90        100        110        120 
CEVEEGDKPD VDKAVEAAQA AFQRGSPWRR LDALSRGQLL HQLADLVERD RAILATLETM 

       130        140        150        160        170        180 
DTGKPFLHAF FVDLEGCIKT FRYFAGWADK IQGRTIPTDD NVVCFTRHEP IGVCGAITPW 

       190        200        210        220        230        240 
NFPLLMLAWK LAPALCCGNT VVLKPAEQTP LTALYLASLI KEVGFPPGVV NIVPGFGPTV 

       250        260        270        280        290        300 
GAAISSHPQI NKIAFTGSTE VGKLVREAAS RSNLKRVTLE LGGKNPCIVC ADADLDLAVE 

       310        320        330        340        350        360 
CAHQGVFFNQ GQCCTAASRV FVEEQVYGEF VRRSVEFAKK RPVGDPFDAK TEQGPQIDQK 

       370        380        390        400        410        420 
QFDKILELIE SGKKEGAKLE CGGSAMEDRG LFIKPTVFSD VTDNMRIAKE EIFGPVQPIL 

       430        440        450        460        470        480 
KFKNLEEVIK RANSTDYGLT AAVFTKNLDK ALKLAAALES GTVWINCYNA FYAQAPFGGF 

       490        500        510 
KMSGNGRELG EYALAEYTEV KTVTIKLEEK NP 

« Hide

References

« Hide 'large scale' references
[1]"A retinoic acid synthesizing enzyme in ventral retina and telencephalon of the embryonic mouse."
Li H., Wagner E., McCaffery P., Smith D., Andreadis A., Drager U.C.
Mech. Dev. 95:283-289(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: B6/D2.
[2]"RALDH3, a retinaldehyde dehydrogenase that generates retinoic acid, is expressed in the ventral retina, otic vesicle and olfactory pit during mouse development."
Mic F.A., Molotkov A., Fan X., Cuenca A.E., Duester G.
Mech. Dev. 97:227-230(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
Strain: C57BL/6 X 129/SvJ.
Tissue: Kidney.
[3]"Identification of RALDH-3, a novel retinaldehyde dehydrogenase, expressed in the ventral region of the retina."
Suzuki R., Shintani T., Sakuta H., Kato A., Ohkawara T., Osumi N., Noda M.
Mech. Dev. 98:37-50(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: C57BL/6J.
Tissue: Retina.
[4]"Aldehyde dehydrogenase 6, a cytosolic retinaldehyde dehydrogenase prominently expressed in sensory neuroepithelia during development."
Grun F., Hirose Y., Kawauchi S., Ogura T., Umesono K.
J. Biol. Chem. 275:41210-41218(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF253409 mRNA. Translation: AAF67736.1.
AF280404 mRNA. Translation: AAF86980.1.
AF246711 mRNA. Translation: AAG38488.1.
AF152359 mRNA. Translation: AAG33935.1.
BC058277 mRNA. Translation: AAH58277.1.
RefSeqNP_444310.3. NM_053080.3.
UniGeneMm.140988.

3D structure databases

ProteinModelPortalQ9JHW9.
SMRQ9JHW9. Positions 20-511.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid208193. 1 interaction.

PTM databases

PhosphoSiteQ9JHW9.

Proteomic databases

PaxDbQ9JHW9.
PRIDEQ9JHW9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000015278; ENSMUSP00000015278; ENSMUSG00000015134.
GeneID56847.
KEGGmmu:56847.
UCSCuc009hhi.2. mouse.

Organism-specific databases

CTD220.
MGIMGI:1861722. Aldh1a3.

Phylogenomic databases

eggNOGCOG1012.
GeneTreeENSGT00550000074289.
HOGENOMHOG000271505.
HOVERGENHBG000097.
InParanoidQ9JHW9.
KOK00129.
OMANSMKIMQ.
OrthoDBEOG7PS1F7.
PhylomeDBQ9JHW9.
TreeFamTF300455.

Enzyme and pathway databases

BRENDA1.2.1.36. 3474.
SABIO-RKQ9JHW9.
UniPathwayUPA00912.

Gene expression databases

ArrayExpressQ9JHW9.
BgeeQ9JHW9.
CleanExMM_ALDH1A3.
GenevestigatorQ9JHW9.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. SSF53720. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio313405.
PROQ9JHW9.
SOURCESearch...

Entry information

Entry nameAL1A3_MOUSE
AccessionPrimary (citable) accession number: Q9JHW9
Secondary accession number(s): Q9EQP7, Q9JI72
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot