Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aldehyde dehydrogenase family 1 member A3

Gene

Aldh1a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system.4 Publications

Catalytic activityi

An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei181 – 1811Transition state stabilizerBy similarity
Active sitei280 – 2801Proton acceptorPROSITE-ProRule annotation
Active sitei314 – 3141NucleophilePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi257 – 2626NADBy similarity

GO - Molecular functioni

  1. 3-chloroallyl aldehyde dehydrogenase activity Source: MGI
  2. aldehyde dehydrogenase (NAD) activity Source: MGI
  3. aldehyde dehydrogenase [NAD(P)+] activity Source: MGI
  4. NAD+ binding Source: MGI
  5. protein homodimerization activity Source: MGI
  6. retinal dehydrogenase activity Source: GO_Central
  7. thyroid hormone binding Source: MGI

GO - Biological processi

  1. embryonic camera-type eye development Source: MGI
  2. embryonic eye morphogenesis Source: MGI
  3. face development Source: MGI
  4. inner ear morphogenesis Source: MGI
  5. locomotory behavior Source: MGI
  6. neuromuscular process controlling balance Source: MGI
  7. nucleus accumbens development Source: MGI
  8. olfactory pit development Source: MGI
  9. optic cup morphogenesis involved in camera-type eye development Source: MGI
  10. positive regulation of apoptotic process Source: MGI
  11. positive regulation of retinoic acid receptor signaling pathway Source: MGI
  12. retinal metabolic process Source: MGI
  13. retinoic acid biosynthetic process Source: MGI
  14. retinoic acid metabolic process Source: MGI
  15. retinol metabolic process Source: UniProtKB-UniPathway
  16. righting reflex Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.2.1.36. 3474.
SABIO-RKQ9JHW9.
UniPathwayiUPA00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde dehydrogenase family 1 member A3 (EC:1.2.1.5)
Alternative name(s):
Aldehyde dehydrogenase 6
Retinaldehyde dehydrogenase 3
Short name:
RALDH-3
Short name:
RalDH3
Gene namesi
Name:Aldh1a3
Synonyms:Aldh6, Raldh3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1861722. Aldh1a3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular vesicular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 512511Aldehyde dehydrogenase family 1 member A3PRO_0000056479Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei378 – 3781N6-acetyllysine; alternateBy similarity
Modified residuei378 – 3781N6-succinyllysine; alternateBy similarity
Modified residuei394 – 3941N6-acetyllysine; alternateBy similarity
Modified residuei394 – 3941N6-succinyllysine; alternateBy similarity
Modified residuei409 – 4091N6-acetyllysine; alternateBy similarity
Modified residuei409 – 4091N6-succinyllysine; alternateBy similarity
Modified residuei421 – 4211N6-acetyllysine; alternateBy similarity
Modified residuei421 – 4211N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9JHW9.
PaxDbiQ9JHW9.
PRIDEiQ9JHW9.

PTM databases

PhosphoSiteiQ9JHW9.

Expressioni

Tissue specificityi

Ventral retina.4 Publications

Developmental stagei

In mouse embryos, RALDH3 expression is first noticed in the ventral optic eminence at E8.75, then in the optic vesicle/cup, otic vesicle and olfactory placode/pit from E9.5 to E11.5.1 Publication

Gene expression databases

BgeeiQ9JHW9.
CleanExiMM_ALDH1A3.
ExpressionAtlasiQ9JHW9. baseline and differential.
GenevestigatoriQ9JHW9.

Interactioni

Protein-protein interaction databases

BioGridi208193. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9JHW9.
SMRiQ9JHW9. Positions 20-511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1012.
GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiQ9JHW9.
KOiK00129.
OMAiSLWRQMD.
OrthoDBiEOG7PS1F7.
PhylomeDBiQ9JHW9.
TreeFamiTF300455.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JHW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTNGAVEN GQPDGKPPAL PRPIRNLEVK FTKIFINNDW HESKSGRKFA
60 70 80 90 100
TYNPSTLEKI CEVEEGDKPD VDKAVEAAQA AFQRGSPWRR LDALSRGQLL
110 120 130 140 150
HQLADLVERD RAILATLETM DTGKPFLHAF FVDLEGCIKT FRYFAGWADK
160 170 180 190 200
IQGRTIPTDD NVVCFTRHEP IGVCGAITPW NFPLLMLAWK LAPALCCGNT
210 220 230 240 250
VVLKPAEQTP LTALYLASLI KEVGFPPGVV NIVPGFGPTV GAAISSHPQI
260 270 280 290 300
NKIAFTGSTE VGKLVREAAS RSNLKRVTLE LGGKNPCIVC ADADLDLAVE
310 320 330 340 350
CAHQGVFFNQ GQCCTAASRV FVEEQVYGEF VRRSVEFAKK RPVGDPFDAK
360 370 380 390 400
TEQGPQIDQK QFDKILELIE SGKKEGAKLE CGGSAMEDRG LFIKPTVFSD
410 420 430 440 450
VTDNMRIAKE EIFGPVQPIL KFKNLEEVIK RANSTDYGLT AAVFTKNLDK
460 470 480 490 500
ALKLAAALES GTVWINCYNA FYAQAPFGGF KMSGNGRELG EYALAEYTEV
510
KTVTIKLEEK NP
Length:512
Mass (Da):56,157
Last modified:October 1, 2000 - v1
Checksum:i5BBC6DEE41E58CFE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911L → V in AAF67736 (PubMed:10906479).Curated
Sequence conflicti208 – 2081Q → R in AAG33935 (PubMed:11013254).Curated
Sequence conflicti223 – 2231V → E in AAF67736 (PubMed:10906479).Curated
Sequence conflicti341 – 3411R → S in AAG33935 (PubMed:11013254).Curated
Sequence conflicti407 – 4071I → R in AAF67736 (PubMed:10906479).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF253409 mRNA. Translation: AAF67736.1.
AF280404 mRNA. Translation: AAF86980.1.
AF246711 mRNA. Translation: AAG38488.1.
AF152359 mRNA. Translation: AAG33935.1.
BC058277 mRNA. Translation: AAH58277.1.
CCDSiCCDS21345.1.
RefSeqiNP_444310.3. NM_053080.3.
UniGeneiMm.140988.

Genome annotation databases

EnsembliENSMUST00000015278; ENSMUSP00000015278; ENSMUSG00000015134.
GeneIDi56847.
KEGGimmu:56847.
UCSCiuc009hhi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF253409 mRNA. Translation: AAF67736.1.
AF280404 mRNA. Translation: AAF86980.1.
AF246711 mRNA. Translation: AAG38488.1.
AF152359 mRNA. Translation: AAG33935.1.
BC058277 mRNA. Translation: AAH58277.1.
CCDSiCCDS21345.1.
RefSeqiNP_444310.3. NM_053080.3.
UniGeneiMm.140988.

3D structure databases

ProteinModelPortaliQ9JHW9.
SMRiQ9JHW9. Positions 20-511.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208193. 1 interaction.

PTM databases

PhosphoSiteiQ9JHW9.

Proteomic databases

MaxQBiQ9JHW9.
PaxDbiQ9JHW9.
PRIDEiQ9JHW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015278; ENSMUSP00000015278; ENSMUSG00000015134.
GeneIDi56847.
KEGGimmu:56847.
UCSCiuc009hhi.2. mouse.

Organism-specific databases

CTDi220.
MGIiMGI:1861722. Aldh1a3.

Phylogenomic databases

eggNOGiCOG1012.
GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiQ9JHW9.
KOiK00129.
OMAiSLWRQMD.
OrthoDBiEOG7PS1F7.
PhylomeDBiQ9JHW9.
TreeFamiTF300455.

Enzyme and pathway databases

UniPathwayiUPA00912.
BRENDAi1.2.1.36. 3474.
SABIO-RKQ9JHW9.

Miscellaneous databases

NextBioi313405.
PROiQ9JHW9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JHW9.
CleanExiMM_ALDH1A3.
ExpressionAtlasiQ9JHW9. baseline and differential.
GenevestigatoriQ9JHW9.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A retinoic acid synthesizing enzyme in ventral retina and telencephalon of the embryonic mouse."
    Li H., Wagner E., McCaffery P., Smith D., Andreadis A., Drager U.C.
    Mech. Dev. 95:283-289(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: B6/D2.
  2. "RALDH3, a retinaldehyde dehydrogenase that generates retinoic acid, is expressed in the ventral retina, otic vesicle and olfactory pit during mouse development."
    Mic F.A., Molotkov A., Fan X., Cuenca A.E., Duester G.
    Mech. Dev. 97:227-230(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: C57BL/6 X 129/SvJ.
    Tissue: Kidney.
  3. "Identification of RALDH-3, a novel retinaldehyde dehydrogenase, expressed in the ventral region of the retina."
    Suzuki R., Shintani T., Sakuta H., Kato A., Ohkawara T., Osumi N., Noda M.
    Mech. Dev. 98:37-50(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Retina.
  4. "Aldehyde dehydrogenase 6, a cytosolic retinaldehyde dehydrogenase prominently expressed in sensory neuroepithelia during development."
    Grun F., Hirose Y., Kawauchi S., Ogura T., Umesono K.
    J. Biol. Chem. 275:41210-41218(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiAL1A3_MOUSE
AccessioniPrimary (citable) accession number: Q9JHW9
Secondary accession number(s): Q9EQP7, Q9JI72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: October 1, 2000
Last modified: March 4, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.