Q9JHW6 (SAT1_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diamine acetyltransferase 1
EC=2.3.1.57

Alternative name(s):
Polyamine N-acetyltransferase 1
Putrescine acetyltransferase
Spermidine/spermine N(1)-acetyltransferase 1
Short name=SSAT
Short name=SSAT-1

Gene names

Name:	SAT1
Synonyms:	SAT

Organism

Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells By similarity.
Catalytic activity	Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.
Pathway	Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Polymorphism	SSAT activity and regulation were altered in C55.7Res cells, an N1,N(12)-bis(ethyl)spermine-resistant derivative of the Chinese hamster ovary cell line C55.7. Basal activity was decreased, and no activity induction resulted from exposure to analogue concentrations, which caused 300-fold enzyme induction in parental cells, was observed. SSAT mRNA levels in the absence and presence of analogue were unchanged, but no SSAT protein was detected in C55.7Res cells.
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Diamine acetyltransferase 1		PRO_0000074590
Regions
	Domain	4 – 170	167	N-acetyltransferase
	Region	27 – 28	2	Substrate binding By similarity
	Region	94 – 96	3	Acetyl-CoA binding By similarity
	Region	102 – 107	6	Acetyl-CoA binding By similarity
	Region	126 – 128	3	Substrate binding By similarity
	Region	133 – 136	4	Acetyl-CoA binding By similarity
	Region	140 – 143	4	Acetyl-CoA binding By similarity
Sites
	Binding site	92	1	Substrate; via carbonyl oxygen By similarity
	Binding site	152	1	Substrate By similarity
Amino acid modifications
	Modified residue	26	1	N6-acetyllysine By similarity
Natural variations
	Natural variant	156	1	L → F in N(1),N(12)-bis(ethyl)spermine-resistant cell line. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9JHW6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C2749F8366CEC6F1	FASTA	171	20,029
	10 20 30 40 50 60 MAKFKIRPAT ASDCSDILRL IKELAKYEYM EDQVMLTEKD LLEDGFGEHP FYHCLIAEVP 70 80 90 100 110 120 KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KNLSQVAMKC 130 140 150 160 170 RCSSMHFLVA EWNEPSINFY KRRGASDLSS EEGWRLFKID KEYLLKMAAE E

References

[1]

"Altered spermidine/spermine N1-acetyltransferase activity as a mechanism of cellular resistance to bis(ethyl)polyamine analogues." McCloskey D.E., Pegg A.E. J. Biol. Chem. 275:28708-28714(2000) [PubMed: 10887189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PHE-156. Tissue: Ovarian carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF281149 mRNA. Translation: AAF86286.1.
RefSeq	NP_001233618.1. NM_001246689.1.
3D structure databases
ProteinModelPortal	Q9JHW6.
SMR	Q9JHW6. Positions 2-171.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100689427.
Phylogenomic databases
HOVERGEN	HBG063175.
Family and domain databases
InterPro	IPR000182. AcTrfase_GCN5-related_dom. IPR016181. Acyl_CoA_acyltransferase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT1_CRIGR

Accession

Primary (citable) accession number: Q9JHW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program