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Protein

Vesicle-associated membrane protein 7

Gene

Vamp7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.4 Publications

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase

GO - Biological processi

  • calcium ion regulated exocytosis Source: UniProtKB
  • endosome to lysosome transport Source: UniProtKB
  • eosinophil degranulation Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • exocytosis Source: UniProtKB
  • Golgi to plasma membrane protein transport Source: Ensembl
  • natural killer cell degranulation Source: Ensembl
  • neutrophil degranulation Source: UniProtKB
  • phagocytosis, engulfment Source: UniProtKB
  • positive regulation of dendrite morphogenesis Source: Ensembl
  • positive regulation of histamine secretion by mast cell Source: Ensembl
  • regulation of protein targeting to vacuolar membrane Source: Ensembl
  • SNARE complex assembly Source: Ensembl
  • triglyceride transport Source: RGD
  • vesicle fusion Source: UniProtKB
  • vesicle fusion with Golgi apparatus Source: RGD
  • vesicle-mediated transport Source: UniProtKB
  • vesicle transport along microtubule Source: UniProtKB

Keywordsi

Biological processExocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-421837 Clathrin derived vesicle budding
R-RNO-432720 Lysosome Vesicle Biogenesis
R-RNO-432722 Golgi Associated Vesicle Biogenesis
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis
R-RNO-9020591 Interleukin-12 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 7
Short name:
VAMP-7
Alternative name(s):
Synaptobrevin-like protein 1
Gene namesi
Name:Vamp7
Synonyms:Sybl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi621558 Vamp7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 188CytoplasmicSequence analysisAdd BLAST187
Transmembranei189 – 209Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini210 – 220VesicularSequence analysisAdd BLAST11

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003160882 – 220Vesicle-associated membrane protein 7Add BLAST219

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei167PhosphoserineBy similarity1
Modified residuei168PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9JHW5
PRIDEiQ9JHW5

PTM databases

PhosphoSitePlusiQ9JHW5
SwissPalmiQ9JHW5

Expressioni

Tissue specificityi

Expressed in brain, kidney, liver, lung, spleen and thymus. Not expressed in heart and skeletal muscle.4 Publications

Gene expression databases

BgeeiENSRNOG00000008372
GenevisibleiQ9JHW5 RN

Interactioni

Subunit structurei

May interact with STX17 (By similarity). Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ANKRD27Q96NW44EBI-919936,EBI-6125599From Homo sapiens.

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase

Protein-protein interaction databases

BioGridi250111, 1 interactor
CORUMiQ9JHW5
DIPiDIP-37175N
IntActiQ9JHW5, 7 interactors
MINTiQ9JHW5
STRINGi10116.ENSRNOP00000011065

Structurei

3D structure databases

ProteinModelPortaliQ9JHW5
SMRiQ9JHW5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 110LonginPROSITE-ProRule annotationAdd BLAST104
Domaini125 – 185v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0859 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00510000047733
HOGENOMiHOG000042711
HOVERGENiHBG006675
InParanoidiQ9JHW5
KOiK08515
OMAiKLTYTHG
OrthoDBiEOG091G0MPE
PhylomeDBiQ9JHW5
TreeFamiTF323448

Family and domain databases

InterProiView protein in InterPro
IPR011012 Longin-like_dom_sf
IPR010908 Longin_dom
IPR001388 Synaptobrevin
PfamiView protein in Pfam
PF13774 Longin, 1 hit
PF00957 Synaptobrevin, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
SMARTiView protein in SMART
SM01270 Longin, 1 hit
SUPFAMiSSF64356 SSF64356, 1 hit
PROSITEiView protein in PROSITE
PS50859 LONGIN, 1 hit
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JHW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY
60 70 80 90 100
LFHYICQDRI VYLCITDDDF ERSRAFGFLN EVKKRFQTTY GSRAQTALPY
110 120 130 140 150
AMNSEFSSVL AAQLKHHSEN QSLDRVTETQ AQVDELKGIM VRNIDLVAQR
160 170 180 190 200
GERLELLIDK TENLVDSSVT FKTTSRNLAR AMCVKNVKLT AIIVVVSIVF
210 220
IYIIVSPLCG GFTWPSCVKK
Length:220
Mass (Da):24,776
Last modified:October 1, 2000 - v1
Checksum:i527D818A23224A54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF281632 mRNA Translation: AAF88059.1
PIRiJC7258
RefSeqiNP_445983.1, NM_053531.1
XP_006249078.1, XM_006249016.3
UniGeneiRn.34151

Genome annotation databases

EnsembliENSRNOT00000011065; ENSRNOP00000011065; ENSRNOG00000008372
GeneIDi85491
KEGGirno:85491
UCSCiRGD:621558 rat

Entry informationi

Entry nameiVAMP7_RAT
AccessioniPrimary (citable) accession number: Q9JHW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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