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Protein

Vesicle-associated membrane protein 7

Gene

Vamp7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.4 Publications

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • syntaxin binding Source: RGD

GO - Biological processi

  • calcium ion regulated exocytosis Source: UniProtKB
  • endosome to lysosome transport Source: UniProtKB
  • eosinophil degranulation Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • exocytosis Source: UniProtKB
  • Golgi to plasma membrane protein transport Source: Ensembl
  • natural killer cell degranulation Source: Ensembl
  • neutrophil degranulation Source: UniProtKB
  • phagocytosis, engulfment Source: UniProtKB
  • positive regulation of dendrite morphogenesis Source: Ensembl
  • positive regulation of histamine secretion by mast cell Source: Ensembl
  • regulation of protein targeting to vacuolar membrane Source: Ensembl
  • SNARE complex assembly Source: Ensembl
  • triglyceride transport Source: RGD
  • vesicle fusion Source: UniProtKB
  • vesicle fusion with Golgi apparatus Source: RGD
  • vesicle-mediated transport Source: UniProtKB
  • vesicle transport along microtubule Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-421837. Clathrin derived vesicle budding.
R-RNO-432720. Lysosome Vesicle Biogenesis.
R-RNO-432722. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 7
Short name:
VAMP-7
Alternative name(s):
Synaptobrevin-like protein 1
Gene namesi
Name:Vamp7
Synonyms:Sybl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi621558. Vamp7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 188187CytoplasmicSequence analysisAdd
BLAST
Transmembranei189 – 20921Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini210 – 22011VesicularSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical part of cell Source: Ensembl
  • azurophil granule membrane Source: Ensembl
  • cell junction Source: UniProtKB-KW
  • cell surface Source: Ensembl
  • endoplasmic reticulum membrane Source: UniProtKB
  • extracellular exosome Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • lamellipodium Source: Ensembl
  • late endosome membrane Source: UniProtKB
  • lysosomal membrane Source: UniProtKB
  • neuron projection Source: UniProtKB
  • perinuclear region of cytoplasm Source: Ensembl
  • phagocytic vesicle Source: UniProtKB
  • phagocytic vesicle membrane Source: UniProtKB-SubCell
  • plasma membrane Source: Ensembl
  • platelet alpha granule Source: Ensembl
  • pseudopodium Source: Ensembl
  • SNARE complex Source: UniProtKB
  • synapse Source: UniProtKB-KW
  • trans-Golgi network Source: Ensembl
  • transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 220219Vesicle-associated membrane protein 7PRO_0000316088Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei168 – 1681PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9JHW5.
PRIDEiQ9JHW5.

PTM databases

SwissPalmiQ9JHW5.

Expressioni

Tissue specificityi

Expressed in brain, kidney, liver, lung, spleen and thymus. Not expressed in heart and skeletal muscle.4 Publications

Gene expression databases

GenevisibleiQ9JHW5. RN.

Interactioni

Subunit structurei

May interact with STX17 (By similarity). Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ANKRD27Q96NW44EBI-919936,EBI-6125599From a different organism.

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • syntaxin binding Source: RGD

Protein-protein interaction databases

BioGridi250111. 1 interaction.
DIPiDIP-37175N.
IntActiQ9JHW5. 3 interactions.
MINTiMINT-4617656.
STRINGi10116.ENSRNOP00000011065.

Structurei

3D structure databases

ProteinModelPortaliQ9JHW5.
SMRiQ9JHW5. Positions 1-122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 110104LonginPROSITE-ProRule annotationAdd
BLAST
Domaini125 – 18561v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 longin domain.PROSITE-ProRule annotation
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0859. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00510000047733.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ9JHW5.
KOiK08515.
OMAiIDNVAMR.
OrthoDBiEOG75B87Z.
PhylomeDBiQ9JHW5.
TreeFamiTF323448.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
SMARTiSM01270. Longin. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JHW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY
60 70 80 90 100
LFHYICQDRI VYLCITDDDF ERSRAFGFLN EVKKRFQTTY GSRAQTALPY
110 120 130 140 150
AMNSEFSSVL AAQLKHHSEN QSLDRVTETQ AQVDELKGIM VRNIDLVAQR
160 170 180 190 200
GERLELLIDK TENLVDSSVT FKTTSRNLAR AMCVKNVKLT AIIVVVSIVF
210 220
IYIIVSPLCG GFTWPSCVKK
Length:220
Mass (Da):24,776
Last modified:October 1, 2000 - v1
Checksum:i527D818A23224A54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF281632 mRNA. Translation: AAF88059.1.
PIRiJC7258.
RefSeqiNP_445983.1. NM_053531.1.
XP_006249078.1. XM_006249016.2.
UniGeneiRn.34151.

Genome annotation databases

EnsembliENSRNOT00000011065; ENSRNOP00000011065; ENSRNOG00000008372.
GeneIDi85491.
KEGGirno:85491.
UCSCiRGD:621558. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF281632 mRNA. Translation: AAF88059.1.
PIRiJC7258.
RefSeqiNP_445983.1. NM_053531.1.
XP_006249078.1. XM_006249016.2.
UniGeneiRn.34151.

3D structure databases

ProteinModelPortaliQ9JHW5.
SMRiQ9JHW5. Positions 1-122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250111. 1 interaction.
DIPiDIP-37175N.
IntActiQ9JHW5. 3 interactions.
MINTiMINT-4617656.
STRINGi10116.ENSRNOP00000011065.

PTM databases

SwissPalmiQ9JHW5.

Proteomic databases

PaxDbiQ9JHW5.
PRIDEiQ9JHW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011065; ENSRNOP00000011065; ENSRNOG00000008372.
GeneIDi85491.
KEGGirno:85491.
UCSCiRGD:621558. rat.

Organism-specific databases

CTDi6845.
RGDi621558. Vamp7.

Phylogenomic databases

eggNOGiKOG0859. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00510000047733.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ9JHW5.
KOiK08515.
OMAiIDNVAMR.
OrthoDBiEOG75B87Z.
PhylomeDBiQ9JHW5.
TreeFamiTF323448.

Enzyme and pathway databases

ReactomeiR-RNO-421837. Clathrin derived vesicle budding.
R-RNO-432720. Lysosome Vesicle Biogenesis.
R-RNO-432722. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

NextBioi617580.
PROiQ9JHW5.

Gene expression databases

GenevisibleiQ9JHW5. RN.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
SMARTiSM01270. Longin. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rat basophilic leukemia cells express syntaxin-3 and VAMP-7 in granule membranes."
    Hibi T., Hirashima N., Nakanishi M.
    Biochem. Biophys. Res. Commun. 271:36-41(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Seven novel mammalian SNARE proteins localize to distinct membrane compartments."
    Advani R.J., Bae H.-R., Bock J.B., Chao D.S., Doung Y.-C., Prekeris R., Yoo J.-S., Scheller R.H.
    J. Biol. Chem. 273:10317-10324(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "VAMP-7 mediates vesicular transport from endosomes to lysosomes."
    Advani R.J., Yang B., Prekeris R., Lee K.C., Klumperman J., Scheller R.H.
    J. Cell Biol. 146:765-776(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. "Subcellular localization of tetanus neurotoxin-insensitive vesicle-associated membrane protein (VAMP)/VAMP7 in neuronal cells: evidence for a novel membrane compartment."
    Coco S., Raposo G., Martinez S., Fontaine J.-J., Takamori S., Zahraoui A., Jahn R., Matteoli M., Louvard D., Galli T.
    J. Neurosci. 19:9803-9812(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events."
    Pryor P.R., Mullock B.M., Bright N.A., Lindsay M.R., Gray S.R., Richardson S.C.W., Stewart A., James D.E., Piper R.C., Luzio J.P.
    EMBO Rep. 5:590-595(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SNARE COMPLEX CHARACTERIZATION.
  6. "Identification of SNAREs involved in synaptotagmin VII-regulated lysosomal exocytosis."
    Rao S.K., Huynh C., Proux-Gillardeaux V., Galli T., Andrews N.W.
    J. Biol. Chem. 279:20471-20479(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SNARE COMPLEX CHARACTERIZATION.
  7. "Vesicle-associated membrane protein 7 is expressed in intestinal ER."
    Siddiqi S.A., Mahan J., Siddiqi S., Gorelick F.S., Mansbach C.M. II
    J. Cell Sci. 119:943-950(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiVAMP7_RAT
AccessioniPrimary (citable) accession number: Q9JHW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.