Q9JHW5 (VAMP7_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle-associated membrane protein 7
Short name=VAMP-7

Alternative name(s):
Synaptobrevin-like protein 1

Gene names

Name:	Vamp7
Synonyms:	Sybl1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Ref.3 Ref.5 Ref.6 Ref.7
Subunit structure	Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells.
Subcellular location	Cytoplasmic vesicle › secretory vesicle membrane; Single-pass type IV membrane protein. Golgi apparatus › trans-Golgi network membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein. Lysosome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Cytoplasmic vesicle › phagosome membrane; Single-pass type IV membrane protein By similarity. Note: In immature neurons expression is localized in vesicular structures in axons and dendrites while in mature neurons it is localized to the somatodendritic region. Colocalizes with Lamp1 in kidney cells. Localization to the endoplasmic reticulum membrane was observed in the intestine but not in liver or kidney. Ref.2 Ref.3 Ref.4 Ref.7
Tissue specificity	Expressed in brain, kidney, liver, lung, spleen and thymus. Not expressed in heart and skeletal muscle. Ref.2 Ref.3 Ref.4 Ref.7
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Endosome Golgi apparatus Lysosome Membrane
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from direct assay Ref.7. Source: UniProtKB calcium ion-dependent exocytosis Inferred from direct assay Ref.6. Source: UniProtKB endosome to lysosome transport Inferred from direct assay Ref.5. Source: UniProtKB eosinophil degranulation Inferred from direct assay Ref.6. Source: UniProtKB neutrophil degranulation Inferred from direct assay Ref.6. Source: UniProtKB phagocytosis, engulfment Inferred from direct assay Ref.5. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW triglyceride transport Inferred from mutant phenotype Ref.7. Source: RGD vesicle fusion with Golgi apparatus Inferred from direct assay. Source: RGD
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell SNARE complex Inferred from direct assay Ref.5. Source: UniProtKB endoplasmic reticulum membrane Inferred from direct assay Ref.7. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW late endosome membrane Inferred from direct assay Ref.3. Source: UniProtKB lysosomal membrane Inferred from direct assay Ref.3. Source: UniProtKB phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell transport vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	syntaxin binding Inferred from physical interaction Ref.5. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 220	219	Vesicle-associated membrane protein 7		PRO_0000316088
Regions
	Topological domain	2 – 188	187	Cytoplasmic Potential
	Transmembrane	189 – 209	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	210 – 220	11	Vesicular Potential
	Domain	7 – 110	104	Longin
	Domain	125 – 185	61	v-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	168	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JHW5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 527D818A23224A54	FASTA	220	24,776
	10 20 30 40 50 60 MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY LFHYICQDRI 70 80 90 100 110 120 VYLCITDDDF ERSRAFGFLN EVKKRFQTTY GSRAQTALPY AMNSEFSSVL AAQLKHHSEN 130 140 150 160 170 180 QSLDRVTETQ AQVDELKGIM VRNIDLVAQR GERLELLIDK TENLVDSSVT FKTTSRNLAR 190 200 210 220 AMCVKNVKLT AIIVVVSIVF IYIIVSPLCG GFTWPSCVKK

References

[1]	"Rat basophilic leukemia cells express syntaxin-3 and VAMP-7 in granule membranes." Hibi T., Hirashima N., Nakanishi M. Biochem. Biophys. Res. Commun. 271:36-41(2000) [PubMed: 10777677] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Seven novel mammalian SNARE proteins localize to distinct membrane compartments." Advani R.J., Bae H.-R., Bock J.B., Chao D.S., Doung Y.-C., Prekeris R., Yoo J.-S., Scheller R.H. J. Biol. Chem. 273:10317-10324(1998) [PubMed: 9553086] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	"VAMP-7 mediates vesicular transport from endosomes to lysosomes." Advani R.J., Yang B., Prekeris R., Lee K.C., Klumperman J., Scheller R.H. J. Cell Biol. 146:765-776(1999) [PubMed: 10459012] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]	"Subcellular localization of tetanus neurotoxin-insensitive vesicle-associated membrane protein (VAMP)/VAMP7 in neuronal cells: evidence for a novel membrane compartment." Coco S., Raposo G., Martinez S., Fontaine J.-J., Takamori S., Zahraoui A., Jahn R., Matteoli M., Louvard D., Galli T. J. Neurosci. 19:9803-9812(1999) [PubMed: 10559389] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events." Pryor P.R., Mullock B.M., Bright N.A., Lindsay M.R., Gray S.R., Richardson S.C.W., Stewart A., James D.E., Piper R.C., Luzio J.P. EMBO Rep. 5:590-595(2004) [PubMed: 15133481] [Abstract] Cited for: FUNCTION, SNARE COMPLEX CHARACTERIZATION.
[6]	"Identification of SNAREs involved in synaptotagmin VII-regulated lysosomal exocytosis." Rao S.K., Huynh C., Proux-Gillardeaux V., Galli T., Andrews N.W. J. Biol. Chem. 279:20471-20479(2004) [PubMed: 14993220] [Abstract] Cited for: FUNCTION, SNARE COMPLEX CHARACTERIZATION.
[7]	"Vesicle-associated membrane protein 7 is expressed in intestinal ER." Siddiqi S.A., Mahan J., Siddiqi S., Gorelick F.S., Mansbach C.M. II J. Cell Sci. 119:943-950(2006) [PubMed: 16495485] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF281632 mRNA. Translation: AAF88059.1.
IPI	IPI00199984.
PIR	JC7258.
RefSeq	NP_445983.1. NM_053531.1.
UniGene	Rn.34151.
3D structure databases
HSSP	HSSP built from PDB template 1GL2 based on UniProtKB Q9WUF4.
ProteinModelPortal	Q9JHW5.
SMR	Q9JHW5. Positions 1-122.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9JHW5. 1 interaction.
STRING	Q9JHW5.
Proteomic databases
PRIDE	Q9JHW5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000011065; ENSRNOP00000011065; ENSRNOG00000008372.
GeneID	85491.
KEGG	rno:85491.
Organism-specific databases
CTD	6845.
RGD	621558. Vamp7.
Phylogenomic databases
eggNOG	maNOG08658.
GeneTree	ENSGT00510000047733.
HOVERGEN	HBG006675.
InParanoid	Q9JHW5.
OMA	LTFLCMA.
OrthoDB	EOG49079V.
PhylomeDB	Q9JHW5.
Gene expression databases
ArrayExpress	Q9JHW5.
Genevestigator	Q9JHW5.
Family and domain databases
InterPro	IPR010908. Longin. IPR011012. Longin-like. IPR001388. Synaptobrevin. [Graphical view]
Gene3D	G3DSA:3.30.450.50. Longin. 1 hit.
KO	K08515.
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
PRINTS	PR00219. SYNAPTOBREVN.
SUPFAM	SSF64356. Longin_like. 1 hit.
PROSITE	PS50859. LONGIN. 1 hit. PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	617580.

Entry information

Entry name

VAMP7_RAT

Accession

Primary (citable) accession number: Q9JHW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program