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Protein

Selenocysteine-specific elongation factor

Gene

Eefsec

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTPSequence analysis8
Nucleotide bindingi78 – 82GTPSequence analysis5
Nucleotide bindingi132 – 136GTPSequence analysis5

GO - Molecular functioni

  • GTPase activity Source: MGI
  • GTP binding Source: MGI
  • ribonucleoprotein complex binding Source: MGI
  • selenocysteine insertion sequence binding Source: MGI
  • translation elongation factor activity Source: MGI
  • tRNA binding Source: MGI

GO - Biological processi

  • selenocysteine incorporation Source: MGI
  • selenocysteine metabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2408557. Selenocysteine synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Selenocysteine-specific elongation factor
Alternative name(s):
Elongation factor sec
Eukaryotic elongation factor, selenocysteine-tRNA-specific
Short name:
mSelB
Gene namesi
Name:Eefsec
Synonyms:Selb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2137092. Eefsec.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • intracellular ribonucleoprotein complex Source: MGI
  • mitochondrion Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000914791 – 583Selenocysteine-specific elongation factorAdd BLAST583

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei524PhosphoserineBy similarity1
Modified residuei532PhosphothreonineCombined sources1
Modified residuei543Omega-N-methylarginineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ9JHW4.
MaxQBiQ9JHW4.
PaxDbiQ9JHW4.
PeptideAtlasiQ9JHW4.
PRIDEiQ9JHW4.

PTM databases

iPTMnetiQ9JHW4.
PhosphoSitePlusiQ9JHW4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033216.
ExpressionAtlasiQ9JHW4. baseline and differential.
GenevisibleiQ9JHW4. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9JHW4. 3 interactors.
MINTiMINT-4134069.
STRINGi10090.ENSMUSP00000131207.

Structurei

3D structure databases

ProteinModelPortaliQ9JHW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 203tr-type GPROSITE-ProRule annotationAdd BLAST199

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 21G1PROSITE-ProRule annotation8
Regioni46 – 50G2PROSITE-ProRule annotation5
Regioni78 – 81G3PROSITE-ProRule annotation4
Regioni132 – 135G4PROSITE-ProRule annotation4
Regioni171 – 173G5PROSITE-ProRule annotation3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi534 – 540Nuclear localization signalSequence analysis7

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. SelB subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0461. Eukaryota.
COG3276. LUCA.
GeneTreeiENSGT00550000074682.
HOGENOMiHOG000247007.
HOVERGENiHBG017788.
InParanoidiQ9JHW4.
KOiK03833.
OMAiGQGTVCT.
OrthoDBiEOG091G0AQO.
TreeFamiTF300432.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JHW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRRVNVNV GVLGHIDSGK TALARALSTT ASTAAFDKQP QSRERGITLD
60 70 80 90 100
LGFSCFVVPL PGAEPGSSDT LLQVTLVDCP GHASLIRTII GGAQIIDLMM
110 120 130 140 150
LVIDVTKGMQ TQSAECLVIG QIACQKLVVV LNKIDLLAEG KRQAAIDKMT
160 170 180 190 200
KKMQKTLENT KFRGAPIIPV AAKPGGPEAP ETEAPQGISE LIELLKSQIS
210 220 230 240 250
IPTRDPSGPF LMSVDHCFSI KGQGTVMTGT ILSGTISLGD SVEIPALKVV
260 270 280 290 300
KKVKSMQMFH TPVTSAMQGD RLGICVTQFD PKLLERGLVC APESLHTVHA
310 320 330 340 350
ALISVEKIPY FRGPLQTKAK FHITVGHETV MGRTLFFSPA PDSFDLEPVL
360 370 380 390 400
DSFDLSREYL FQEQYLCKDS MPTATEGDDE ADPKAGHAPG GHCPRQQWAL
410 420 430 440 450
VEFEKPVTCP RLCLVIGSRL DADIHTNTCR LAFHGVLLQG LEDKNYIESF
460 470 480 490 500
LPALRVYKLK HKHGLVERVM DDYSVIGRSL FKKETNIQLF VGLKVQLSTG
510 520 530 540 550
EQGIIDSAFG QSGKFKIHIP GGLSPESKKI LTPTLKKRSR AGRGETTKPE
560 570 580
EGTERPEPIQ PVTLNLSFKR YVFDTQKRMV QTP
Length:583
Mass (Da):63,539
Last modified:July 27, 2011 - v2
Checksum:i2960437B1D82DAE1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162F → S in AAF91471 (PubMed:11265756).Curated1
Sequence conflicti457Y → T in AAF91471 (PubMed:11265756).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF283518 mRNA. Translation: AAF91471.1.
AF268871 mRNA. Translation: AAG13374.1.
AK028424 mRNA. Translation: BAC25942.1.
CH466523 Genomic DNA. Translation: EDK99245.1.
BC024915 mRNA. Translation: AAH24915.1.
CCDSiCCDS20335.1.
RefSeqiNP_075547.1. NM_023060.3.
UniGeneiMm.333237.

Genome annotation databases

EnsembliENSMUST00000165242; ENSMUSP00000131207; ENSMUSG00000033216.
GeneIDi65967.
KEGGimmu:65967.
UCSCiuc009cvg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF283518 mRNA. Translation: AAF91471.1.
AF268871 mRNA. Translation: AAG13374.1.
AK028424 mRNA. Translation: BAC25942.1.
CH466523 Genomic DNA. Translation: EDK99245.1.
BC024915 mRNA. Translation: AAH24915.1.
CCDSiCCDS20335.1.
RefSeqiNP_075547.1. NM_023060.3.
UniGeneiMm.333237.

3D structure databases

ProteinModelPortaliQ9JHW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JHW4. 3 interactors.
MINTiMINT-4134069.
STRINGi10090.ENSMUSP00000131207.

PTM databases

iPTMnetiQ9JHW4.
PhosphoSitePlusiQ9JHW4.

Proteomic databases

EPDiQ9JHW4.
MaxQBiQ9JHW4.
PaxDbiQ9JHW4.
PeptideAtlasiQ9JHW4.
PRIDEiQ9JHW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000165242; ENSMUSP00000131207; ENSMUSG00000033216.
GeneIDi65967.
KEGGimmu:65967.
UCSCiuc009cvg.1. mouse.

Organism-specific databases

CTDi60678.
MGIiMGI:2137092. Eefsec.

Phylogenomic databases

eggNOGiKOG0461. Eukaryota.
COG3276. LUCA.
GeneTreeiENSGT00550000074682.
HOGENOMiHOG000247007.
HOVERGENiHBG017788.
InParanoidiQ9JHW4.
KOiK03833.
OMAiGQGTVCT.
OrthoDBiEOG091G0AQO.
TreeFamiTF300432.

Enzyme and pathway databases

ReactomeiR-MMU-2408557. Selenocysteine synthesis.

Miscellaneous databases

PROiQ9JHW4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033216.
ExpressionAtlasiQ9JHW4. baseline and differential.
GenevisibleiQ9JHW4. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSELB_MOUSE
AccessioniPrimary (citable) accession number: Q9JHW4
Secondary accession number(s): Q6GTZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.