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Protein

Selenocysteine-specific elongation factor

Gene

Eefsec

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTPSequence analysis8
Nucleotide bindingi78 – 82GTPSequence analysis5
Nucleotide bindingi132 – 136GTPSequence analysis5

GO - Molecular functioni

  • GTPase activity Source: MGI
  • GTP binding Source: MGI
  • ribonucleoprotein complex binding Source: MGI
  • selenocysteine insertion sequence binding Source: MGI
  • translation elongation factor activity Source: MGI
  • tRNA binding Source: MGI

GO - Biological processi

  • selenocysteine incorporation Source: MGI

Keywordsi

Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Selenocysteine-specific elongation factor
Alternative name(s):
Elongation factor sec
Eukaryotic elongation factor, selenocysteine-tRNA-specific
Short name:
mSelB
Gene namesi
Name:Eefsec
Synonyms:Selb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2137092 Eefsec

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000914791 – 583Selenocysteine-specific elongation factorAdd BLAST583

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei524PhosphoserineBy similarity1
Modified residuei532PhosphothreonineCombined sources1
Modified residuei543Omega-N-methylarginineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ9JHW4
MaxQBiQ9JHW4
PaxDbiQ9JHW4
PeptideAtlasiQ9JHW4
PRIDEiQ9JHW4

PTM databases

iPTMnetiQ9JHW4
PhosphoSitePlusiQ9JHW4

Expressioni

Gene expression databases

BgeeiENSMUSG00000033216
ExpressionAtlasiQ9JHW4 baseline and differential
GenevisibleiQ9JHW4 MM

Interactioni

Protein-protein interaction databases

IntActiQ9JHW4 3 interactors.
MINTiQ9JHW4
STRINGi10090.ENSMUSP00000131207

Structurei

3D structure databases

ProteinModelPortaliQ9JHW4
SMRiQ9JHW4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 203tr-type GPROSITE-ProRule annotationAdd BLAST199

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 21G1PROSITE-ProRule annotation8
Regioni46 – 50G2PROSITE-ProRule annotation5
Regioni78 – 81G3PROSITE-ProRule annotation4
Regioni132 – 135G4PROSITE-ProRule annotation4
Regioni171 – 173G5PROSITE-ProRule annotation3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi534 – 540Nuclear localization signalSequence analysis7

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. SelB subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0461 Eukaryota
COG3276 LUCA
GeneTreeiENSGT00550000074682
HOGENOMiHOG000247007
HOVERGENiHBG017788
InParanoidiQ9JHW4
KOiK03833
OMAiGQGTVCT
OrthoDBiEOG091G0AQO
TreeFamiTF300432

Family and domain databases

InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JHW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRRVNVNV GVLGHIDSGK TALARALSTT ASTAAFDKQP QSRERGITLD
60 70 80 90 100
LGFSCFVVPL PGAEPGSSDT LLQVTLVDCP GHASLIRTII GGAQIIDLMM
110 120 130 140 150
LVIDVTKGMQ TQSAECLVIG QIACQKLVVV LNKIDLLAEG KRQAAIDKMT
160 170 180 190 200
KKMQKTLENT KFRGAPIIPV AAKPGGPEAP ETEAPQGISE LIELLKSQIS
210 220 230 240 250
IPTRDPSGPF LMSVDHCFSI KGQGTVMTGT ILSGTISLGD SVEIPALKVV
260 270 280 290 300
KKVKSMQMFH TPVTSAMQGD RLGICVTQFD PKLLERGLVC APESLHTVHA
310 320 330 340 350
ALISVEKIPY FRGPLQTKAK FHITVGHETV MGRTLFFSPA PDSFDLEPVL
360 370 380 390 400
DSFDLSREYL FQEQYLCKDS MPTATEGDDE ADPKAGHAPG GHCPRQQWAL
410 420 430 440 450
VEFEKPVTCP RLCLVIGSRL DADIHTNTCR LAFHGVLLQG LEDKNYIESF
460 470 480 490 500
LPALRVYKLK HKHGLVERVM DDYSVIGRSL FKKETNIQLF VGLKVQLSTG
510 520 530 540 550
EQGIIDSAFG QSGKFKIHIP GGLSPESKKI LTPTLKKRSR AGRGETTKPE
560 570 580
EGTERPEPIQ PVTLNLSFKR YVFDTQKRMV QTP
Length:583
Mass (Da):63,539
Last modified:July 27, 2011 - v2
Checksum:i2960437B1D82DAE1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162F → S in AAF91471 (PubMed:11265756).Curated1
Sequence conflicti457Y → T in AAF91471 (PubMed:11265756).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF283518 mRNA Translation: AAF91471.1
AF268871 mRNA Translation: AAG13374.1
AK028424 mRNA Translation: BAC25942.1
CH466523 Genomic DNA Translation: EDK99245.1
BC024915 mRNA Translation: AAH24915.1
CCDSiCCDS20335.1
RefSeqiNP_075547.1, NM_023060.3
UniGeneiMm.333237

Genome annotation databases

EnsembliENSMUST00000165242; ENSMUSP00000131207; ENSMUSG00000033216
GeneIDi65967
KEGGimmu:65967
UCSCiuc009cvg.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSELB_MOUSE
AccessioniPrimary (citable) accession number: Q9JHW4
Secondary accession number(s): Q6GTZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: February 28, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome