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Protein

Carboxypeptidase D

Gene

Cpd

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Releases C-terminal Arg and Lys from polypeptides.By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Zinc 1; catalyticBy similarity1
Metal bindingi142Zinc 1; catalyticBy similarity1
Metal bindingi257Zinc 1; catalyticBy similarity1
Metal bindingi564Zinc 2; catalyticBy similarity1
Metal bindingi567Zinc 2; catalyticBy similarity1
Metal bindingi671Zinc 2; catalyticBy similarity1
Active sitei762Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.22 5301

Protein family/group databases

MEROPSiM14.011

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase D (EC:3.4.17.22)
Alternative name(s):
Metallocarboxypeptidase D
gp180
Gene namesi
Name:CpdImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi2393 Cpd

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 1297ExtracellularSequence analysisAdd BLAST1260
Transmembranei1298 – 1318HelicalSequence analysisAdd BLAST21
Topological domaini1319 – 1378CytoplasmicSequence analysisAdd BLAST60

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
ChainiPRO_000000440338 – 1378Carboxypeptidase DSequence analysisAdd BLAST1341

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi172N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi217N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei265PhosphotyrosineBy similarity1
Modified residuei270PhosphoserineBy similarity1
Glycosylationi399N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi410N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi429N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi522N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi626N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi811N-linked (GlcNAc...) asparagine; alternateBy similarity1
Glycosylationi811N-linked (HexNAc...) asparagine; alternateCombined sources1
Glycosylationi855N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi867N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi879N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi953N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi976N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1068N-linked (GlcNAc...) asparagine; alternateSequence analysis1
Glycosylationi1068N-linked (HexNAc...) asparagine; alternateCombined sources1
Glycosylationi1140N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi1315S-palmitoyl cysteineBy similarity1
Lipidationi1319S-palmitoyl cysteineBy similarity1
Lipidationi1321S-palmitoyl cysteineBy similarity1
Modified residuei1356PhosphoserineBy similarity1
Modified residuei1359PhosphoserineBy similarity1
Modified residuei1366PhosphothreonineCombined sources1
Modified residuei1368PhosphothreonineCombined sources1

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9JHW1
PRIDEiQ9JHW1

PTM databases

iPTMnetiQ9JHW1
PhosphoSitePlusiQ9JHW1
SwissPalmiQ9JHW1
UniCarbKBiQ9JHW1

Expressioni

Tissue specificityi

Isoform 1 is widely expressed with highest levels in the hippocampus, spinal cord, atrium, colon, testis and ovaries. Detected in the liver of females but not males. Isoform 2 is not detected in brain or lung.2 Publications

Inductioni

Isoform 2 is up-regulated by exposure to prolactin or interleukin-2.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005262

Structurei

3D structure databases

ProteinModelPortaliQ9JHW1
SMRiQ9JHW1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 492Carboxypeptidase-like 1Add BLAST455
Regioni494 – 897Carboxypeptidase-like 2Add BLAST404
Regioni898 – 1297Carboxypeptidase-like 3Add BLAST400

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi162 – 164Cell attachment siteSequence analysis3

Domaini

There are 3 carboxypeptidase-like domains. Only the first two domains seem to have kept a catalytic activity.1 Publication

Sequence similaritiesi

Belongs to the peptidase M14 family.Sequence analysis

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2649 Eukaryota
ENOG410XX0H LUCA
HOGENOMiHOG000046445
HOVERGENiHBG006932
InParanoidiQ9JHW1
KOiK07752
PhylomeDBiQ9JHW1

Family and domain databases

CDDicd03868 M14_CPD_I, 1 hit
cd03863 M14_CPD_II, 1 hit
InterProiView protein in InterPro
IPR008969 CarboxyPept-like_regulatory
IPR034241 M14_CPD_I
IPR034224 M14_CPD_II
IPR015567 Pept_M14B_carboxypept_D2
IPR000834 Peptidase_M14
PANTHERiPTHR11532:SF73 PTHR11532:SF73, 4 hits
PfamiView protein in Pfam
PF00246 Peptidase_M14, 3 hits
PRINTSiPR00765 CRBOXYPTASEA
SMARTiView protein in SMART
SM00631 Zn_pept, 3 hits
SUPFAMiSSF49464 SSF49464, 3 hits
PROSITEiView protein in PROSITE
PS00132 CARBOXYPEPT_ZN_1, 2 hits
PS00133 CARBOXYPEPT_ZN_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q9JHW1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASGWDERPP WRLESLRLLP PPPLLLLLLL LRSSAQAAHI KKAEATTTTV
60 70 80 90 100
GGSEAAEGQF DHYYHEAALG EALEAAAAAG PPGLARLFSI GNSVEGRPLW
110 120 130 140 150
VLRLTAGLGP PPTPAAVGLD AAGPLLPGRP QVKLVGNMHG DETVSRQVLV
160 170 180 190 200
YLARELASGY RRGDPRLVRL LNTTDVYLLP SLNPDGFERA REGDCGLGDS
210 220 230 240 250
GPPGTSGRDN SRGRDLNRSF PDQFSTGEPP SLDEVPEVRA LIDWIRRNKF
260 270 280 290 300
VLSGNLHGGS VVASYPFDDS PEHKTTGIYS KTSDDEVFRY LAKAYASNHP
310 320 330 340 350
IMRTGEPHCP GDEEETFKDG ITNGAHWYDV EGGMQDYNYV WANCFEITLE
360 370 380 390 400
LSCCKYPPAS QLRQEWENNR ESLITLIEKV HIGIKGFVKD SVTGSGLENA
410 420 430 440 450
TISVAGINHN ITTGRFGDFH RLLIPGSYNL TAVSPGYMPL TINNIVVKEG
460 470 480 490 500
PATEIDFSLQ PTVMSVVPDS TEAVTTPGTV AVPNIPPGTP SSHQPIQPKD
510 520 530 540 550
FHHHHFPDME IFLRRFANEY PNITRLYSLG KSVESRELYV MEISDNPGVH
560 570 580 590 600
EPGEPEFKYI GNMHGNEVVG RELLLNLIEY LCKNFGTDPE VTDLVRSTRI
610 620 630 640 650
HLMPSMNPDG YEKSQEGDSI SVVGRNNSNN FDLNRNFPDQ FVPITDPTQP
660 670 680 690 700
ETIAVMSWVK AYPFVLSANL HGGSLVVNYP YDDNEQGVAT YSKSPDDAVF
710 720 730 740 750
QQIALSYSKE NSQMFQGRPC KDMYLNEYFP HGITNGASWY NVPGGMQDWN
760 770 780 790 800
YLQTNCFEVT IELGCVKYPF EKELPKYWEQ NRRSLIQFMK QVHQGVKGFV
810 820 830 840 850
LDATDGRGIL NATLSVAEIN HPVTTYKAGD YWRLLVPGTY KITASARGYN
860 870 880 890 900
PVTKNVTVRS EGAIQVNFTL VRSSTDANNE SKKGKGHSTS TDDTSDPTSK
910 920 930 940 950
EFEALIKHLS AENGLEGFML SSSSDLALYR YHSYKDLSEF LRGLVMNYPH
960 970 980 990 1000
ITNLTTLGQS VEYRHIWSLE ISNKPNISEP EEPKIRFVAG IHGNAPVGTE
1010 1020 1030 1040 1050
LLLALAEFLC LNYKKNPVVT QLVDRTRIVI VPSLNPDGRE RAQEKDCTSK
1060 1070 1080 1090 1100
TGHTNARGRD LDTDFTSNAS QPETKAIIEN LIQKQDFSLS IALDGGSVLV
1110 1120 1130 1140 1150
TYPYDKPVQT VENKETLKHL ASLYANNHPS MHMGQPSCPN NSDENIPGGV
1160 1170 1180 1190 1200
MRGAEWHSHL GSMKDYSVTY GHCPEITVYT SCCYFPSAAQ LPALWAENKK
1210 1220 1230 1240 1250
SLLSMLVEVH KGVHGLVKDK TGKPISKAVI VLNEGIRVHT KEGGYFHVLL
1260 1270 1280 1290 1300
APGVHNINAI ADGYQQQHSQ VFVHHDAASS VVIVFDTDNR IFGLPRELVV
1310 1320 1330 1340 1350
TVSGATMSAL ILTACIIWCI CSIKSNRHKD GFHRLRQHHD EYEDEIRMMS
1360 1370
TGSKKSLLSH EFQDETDTEE ETLYSSKH
Length:1,378
Mass (Da):152,616
Last modified:April 12, 2005 - v2
Checksum:i5F7A8DC267ED2DDD
GO
Isoform 21 Publication (identifier: Q9JHW1-2) [UniParc]FASTAAdd to basket
Also known as: CPD-N1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-245: Missing.
     246-249: RRNK → MSQR

Show »
Length:1,133
Mass (Da):126,436
Checksum:i7CE78DCF627F95BA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti455I → M in AAF91481 (PubMed:11181555).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0517121 – 245Missing in isoform 2. 1 PublicationAdd BLAST245
Alternative sequenceiVSP_051713246 – 249RRNK → MSQR in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62897 mRNA Translation: AAB70456.1
AF284830 mRNA Translation: AAF91481.1
RefSeqiNP_036968.1, NM_012836.2 [Q9JHW1-1]
UniGeneiRn.25905

Genome annotation databases

GeneIDi25306
KEGGirno:25306

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiCBPD_RAT
AccessioniPrimary (citable) accession number: Q9JHW1
Secondary accession number(s): O35850
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: May 23, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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