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Q9JHU3

- CDK20_MOUSE

UniProt

Q9JHU3 - CDK20_MOUSE

Protein

Cyclin-dependent kinase 20

Gene

Cdk20

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160' By similarity. Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling.By similarity1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. protein binding Source: MGI

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 20 (EC:2.7.11.22)
    Alternative name(s):
    CDK-activating kinase p42
    Short name:
    CAK-kinase p42
    CDK-related protein kinase PNQLARE
    Cell cycle-related kinase
    Cell division protein kinase 20
    Cyclin-dependent protein kinase H
    Cyclin-kinase-activating kinase p42
    Gene namesi
    Name:Cdk20
    Synonyms:Ccrk, Cdch
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:2145349. Cdk20.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication. Cell projectioncilium 1 Publication

    GO - Cellular componenti

    1. cilium Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection, Cilium, Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 346346Cyclin-dependent kinase 20PRO_0000085703Add
    BLAST

    Proteomic databases

    PaxDbiQ9JHU3.
    PRIDEiQ9JHU3.

    PTM databases

    PhosphoSiteiQ9JHU3.

    Expressioni

    Gene expression databases

    BgeeiQ9JHU3.
    CleanExiMM_CCRK.
    GenevestigatoriQ9JHU3.

    Interactioni

    Subunit structurei

    Monomer By similarity. Interacts with MAK By similarity. Interacts with TBC1D32.By similarity1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JHU3.
    SMRiQ9JHU3. Positions 1-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 288285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115348.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    InParanoidiQ9JHU3.
    KOiK08817.
    OMAiRPFIPEG.
    OrthoDBiEOG75J0NH.
    PhylomeDBiQ9JHU3.
    TreeFamiTF327240.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JHU3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDQYCILGRI GEGAHGIVFK AKHVETGEIV ALKKVALRRL EDGIPNQALR    50
    EIKALQEIED SQYVVQLKAV FPHGAGFVLA FEFMLSDLAE VVRHAQRPLA 100
    PAQVKSYLQM LLKGVAFCHA NNIVHRDLKP ANLLISASGQ LKIADFGLAR 150
    VFSPDGGRLY THQVATRWYR APELLYGARQ YDQGVDLWAV GCIMGELLNG 200
    SPLFPGENDI EQLCCVLRIL GTPSPRVWPE ITELPDYNKI SFKEQAPVPL 250
    EEVLPDASPQ ALDLLGQFLL YPPRQRIAAS QALLHQYFFT APLPAHPSEL 300
    PIPQRPGGPA PKAHPGPPHV HDFHVDRPLE ESLLNPELIR PFIPEG 346
    Length:346
    Mass (Da):38,379
    Last modified:October 1, 2000 - v1
    Checksum:iACCACE0C3FC4B833
    GO
    Isoform 2 (identifier: Q9JHU3-2) [UniParc]FASTAAdd to Basket

    Also known as: Cardiac CCRK

    The sequence of this isoform differs from the canonical sequence as follows:
         168-243: WYRAPELLYG...LPDYNKISFK → SSLSCRTTTR...IHLSCRFLSV
         244-346: Missing.

    Show »
    Length:243
    Mass (Da):26,974
    Checksum:iB9EFCE73BAC18AC0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti44 – 441I → F in AAW82351. 1 PublicationCurated
    Sequence conflicti58 – 581I → M in AAW82351. 1 PublicationCurated
    Sequence conflicti61 – 611S → N in AAW82351. 1 PublicationCurated
    Sequence conflicti75 – 751A → G in AAW82351. 1 PublicationCurated
    Sequence conflicti101 – 1011P → Q in AAW82351. 1 PublicationCurated
    Sequence conflicti157 – 1571G → S in AAW82351. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei168 – 24376WYRAP…KISFK → SSLSCRTTTRSPLRSRCPCP WRRCCLTSLPRHWICWVNSF STLLTSASQLPRLSSISTSS QLPCLPIHLSCRFLSV in isoform 2. 1 PublicationVSP_016753Add
    BLAST
    Alternative sequencei244 – 346103Missing in isoform 2. 1 PublicationVSP_016754Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY005133 mRNA. Translation: AAF89089.1.
    AY904369 mRNA. Translation: AAW82351.1.
    BC031907 mRNA. Translation: AAH31907.1.
    CCDSiCCDS26601.1. [Q9JHU3-1]
    RefSeqiNP_444410.1. NM_053180.2. [Q9JHU3-1]
    UniGeneiMm.74982.

    Genome annotation databases

    EnsembliENSMUST00000021939; ENSMUSP00000021939; ENSMUSG00000021483. [Q9JHU3-1]
    GeneIDi105278.
    KEGGimmu:105278.
    UCSCiuc007qyx.1. mouse. [Q9JHU3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY005133 mRNA. Translation: AAF89089.1 .
    AY904369 mRNA. Translation: AAW82351.1 .
    BC031907 mRNA. Translation: AAH31907.1 .
    CCDSi CCDS26601.1. [Q9JHU3-1 ]
    RefSeqi NP_444410.1. NM_053180.2. [Q9JHU3-1 ]
    UniGenei Mm.74982.

    3D structure databases

    ProteinModelPortali Q9JHU3.
    SMRi Q9JHU3. Positions 1-334.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9JHU3.

    Proteomic databases

    PaxDbi Q9JHU3.
    PRIDEi Q9JHU3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021939 ; ENSMUSP00000021939 ; ENSMUSG00000021483 . [Q9JHU3-1 ]
    GeneIDi 105278.
    KEGGi mmu:105278.
    UCSCi uc007qyx.1. mouse. [Q9JHU3-1 ]

    Organism-specific databases

    CTDi 23552.
    MGIi MGI:2145349. Cdk20.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115348.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    InParanoidi Q9JHU3.
    KOi K08817.
    OMAi RPFIPEG.
    OrthoDBi EOG75J0NH.
    PhylomeDBi Q9JHU3.
    TreeFami TF327240.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 3474.

    Miscellaneous databases

    NextBioi 357580.
    PROi Q9JHU3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JHU3.
    CleanExi MM_CCRK.
    Genevestigatori Q9JHU3.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PNQALRE a novel member of the CDK family."
      Liao J.C., Fisher R.P.
      Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: 129/SvJ.
    2. "Mouse p42(CCRK), cardiac variant."
      Qiu H., Depre C.
      Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Heart.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Eye.
    4. "Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction."
      Ko H.W., Norman R.X., Tran J., Fuller K.P., Fukuda M., Eggenschwiler J.T.
      Dev. Cell 18:237-247(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TBC1D32, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCDK20_MOUSE
    AccessioniPrimary (citable) accession number: Q9JHU3
    Secondary accession number(s): Q5EDC2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3