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Q9JHU3 (CDK20_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 20
EC=2.7.11.22
Alternative name(s):
CDK-activating kinase p42
Short name=CAK-kinase p42
CDK-related protein kinase PNQLARE
Cell cycle-related kinase
Cell division protein kinase 20
Cyclin-dependent protein kinase H
Cyclin-kinase-activating kinase p42
Gene names
Name:Cdk20
Synonyms:Ccrk, Cdch
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160' By similarity. Required for high-level Shh responses in the developing neural tube. Together with BROMI, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling. Ref.4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Monomer By similarity. Interacts with MAK By similarity. Interacts with BROMI. Ref.4

Subcellular location

Nucleus. Cytoplasm. Cell projection › cilium Ref.4.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JHU3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JHU3-2)

Also known as: Cardiac CCRK;

The sequence of this isoform differs from the canonical sequence as follows:
     168-243: WYRAPELLYG...LPDYNKISFK → SSLSCRTTTR...IHLSCRFLSV
     244-346: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Cyclin-dependent kinase 20
PRO_0000085703

Regions

Domain4 – 288285Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1271Proton acceptor By similarity
Binding site331ATP By similarity

Natural variations

Alternative sequence168 – 24376WYRAP…KISFK → SSLSCRTTTRSPLRSRCPCP WRRCCLTSLPRHWICWVNSF STLLTSASQLPRLSSISTSS QLPCLPIHLSCRFLSV in isoform 2.
VSP_016753
Alternative sequence244 – 346103Missing in isoform 2.
VSP_016754

Experimental info

Sequence conflict441I → F in AAW82351. Ref.2
Sequence conflict581I → M in AAW82351. Ref.2
Sequence conflict611S → N in AAW82351. Ref.2
Sequence conflict751A → G in AAW82351. Ref.2
Sequence conflict1011P → Q in AAW82351. Ref.2
Sequence conflict1571G → S in AAW82351. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: ACCACE0C3FC4B833

FASTA34638,379
        10         20         30         40         50         60 
MDQYCILGRI GEGAHGIVFK AKHVETGEIV ALKKVALRRL EDGIPNQALR EIKALQEIED 

        70         80         90        100        110        120 
SQYVVQLKAV FPHGAGFVLA FEFMLSDLAE VVRHAQRPLA PAQVKSYLQM LLKGVAFCHA 

       130        140        150        160        170        180 
NNIVHRDLKP ANLLISASGQ LKIADFGLAR VFSPDGGRLY THQVATRWYR APELLYGARQ 

       190        200        210        220        230        240 
YDQGVDLWAV GCIMGELLNG SPLFPGENDI EQLCCVLRIL GTPSPRVWPE ITELPDYNKI 

       250        260        270        280        290        300 
SFKEQAPVPL EEVLPDASPQ ALDLLGQFLL YPPRQRIAAS QALLHQYFFT APLPAHPSEL 

       310        320        330        340 
PIPQRPGGPA PKAHPGPPHV HDFHVDRPLE ESLLNPELIR PFIPEG

« Hide

Isoform 2 (Cardiac CCRK) [UniParc].

Checksum: B9EFCE73BAC18AC0
Show »

FASTA24326,974

References

« Hide 'large scale' references
[1]"PNQALRE a novel member of the CDK family."
Liao J.C., Fisher R.P.
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: 129/SvJ.
[2]"Mouse p42(CCRK), cardiac variant."
Qiu H., Depre C.
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Heart.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Eye.
[4]"Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction."
Ko H.W., Norman R.X., Tran J., Fuller K.P., Fukuda M., Eggenschwiler J.T.
Dev. Cell 18:237-247(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDK20, MASS SPECTROMETRY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY005133 mRNA. Translation: AAF89089.1.
AY904369 mRNA. Translation: AAW82351.1.
BC031907 mRNA. Translation: AAH31907.1.
IPIIPI00119872.
IPI00553299.
RefSeqNP_444410.1. NM_053180.2.
UniGeneMm.74982.

3D structure databases

ProteinModelPortalQ9JHU3.
SMRQ9JHU3. Positions 1-334.
ModBaseSearch...

PTM databases

PhosphoSiteQ9JHU3.

Proteomic databases

PaxDbQ9JHU3.
PRIDEQ9JHU3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021939; ENSMUSP00000021939; ENSMUSG00000021483.
GeneID105278.
KEGGmmu:105278.
UCSCuc007qyx.1. mouse.

Organism-specific databases

CTD23552.
MGIMGI:2145349. Cdk20.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00680000099989.
HOGENOMHOG000233024.
HOVERGENHBG014652.
InParanoidQ9JHU3.
KOK08817.
OMAAFTHGVP.
OrthoDBEOG4DBTDX.

Enzyme and pathway databases

BRENDA2.7.11.22. 3474.

Gene expression databases

BgeeQ9JHU3.
CleanExMM_CCRK.
GenevestigatorQ9JHU3.
GermOnlineENSMUSG00000021483. Mus musculus.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio357580.
SOURCESearch...

Entry information

Entry nameCDK20_MOUSE
AccessionPrimary (citable) accession number: Q9JHU3
Secondary accession number(s): Q5EDC2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2000
Last modified: May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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