Reviewed, UniProtKB/Swiss-Prot Q9JHU3 (CDK20_MOUSE)

Names and origin

Protein names

Recommended name:
    Cell division protein kinase 20
    EC=2.7.11.22
Alternative name(s):
    Cell cycle-related kinase
    Cyclin-kinase-activating kinase p42
    CDK-activating kinase p42
      Short name=CAK-kinase p42
    CDK-related protein kinase PNQLARE
    Cyclin-dependent protein kinase H

Gene names

Name:	Cdk20
Synonyms:	Ccrk, Cdch

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160' By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Monomer By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9JHU3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JHU3-2)

Also known as: Cardiac CCRK;

The sequence of this isoform differs from the canonical sequence as follows:
     168-243: WYRAPELLYG...LPDYNKISFK → SSLSCRTTTR...IHLSCRFLSV
     244-346: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Cell division protein kinase 20		PRO_0000085703
Regions
	Domain	4 – 288	285	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Natural variations
	Alternative sequence	168 – 243	76	WYRAP…KISFK → SSLSCRTTTRSPLRSRCPCP WRRCCLTSLPRHWICWVNSF STLLTSASQLPRLSSISTSS QLPCLPIHLSCRFLSV in isoform 2.		VSP_016753
	Alternative sequence	244 – 346	103	Missing in isoform 2.		VSP_016754
Experimental info
	Sequence conflict	44	1	I → F in AAW82351. Ref.2
	Sequence conflict	58	1	I → M in AAW82351. Ref.2
	Sequence conflict	61	1	S → N in AAW82351. Ref.2
	Sequence conflict	75	1	A → G in AAW82351. Ref.2
	Sequence conflict	101	1	P → Q in AAW82351. Ref.2
	Sequence conflict	157	1	G → S in AAW82351. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: ACCACE0C3FC4B833	FASTA	346	38,379
	10 20 30 40 50 60 MDQYCILGRI GEGAHGIVFK AKHVETGEIV ALKKVALRRL EDGIPNQALR EIKALQEIED 70 80 90 100 110 120 SQYVVQLKAV FPHGAGFVLA FEFMLSDLAE VVRHAQRPLA PAQVKSYLQM LLKGVAFCHA 130 140 150 160 170 180 NNIVHRDLKP ANLLISASGQ LKIADFGLAR VFSPDGGRLY THQVATRWYR APELLYGARQ 190 200 210 220 230 240 YDQGVDLWAV GCIMGELLNG SPLFPGENDI EQLCCVLRIL GTPSPRVWPE ITELPDYNKI 250 260 270 280 290 300 SFKEQAPVPL EEVLPDASPQ ALDLLGQFLL YPPRQRIAAS QALLHQYFFT APLPAHPSEL 310 320 330 340 PIPQRPGGPA PKAHPGPPHV HDFHVDRPLE ESLLNPELIR PFIPEG
	Isoform 2 (Cardiac CCRK). Checksum: B9EFCE73BAC18AC0	FASTA	243	26,974
	10 20 30 40 50 60 MDQYCILGRI GEGAHGIVFK AKHVETGEIV ALKKVALRRL EDGIPNQALR EIKALQEIED 70 80 90 100 110 120 SQYVVQLKAV FPHGAGFVLA FEFMLSDLAE VVRHAQRPLA PAQVKSYLQM LLKGVAFCHA 130 140 150 160 170 180 NNIVHRDLKP ANLLISASGQ LKIADFGLAR VFSPDGGRLY THQVATRSSL SCRTTTRSPL 190 200 210 220 230 240 RSRCPCPWRR CCLTSLPRHW ICWVNSFSTL LTSASQLPRL SSISTSSQLP CLPIHLSCRF LSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PNQALRE a novel member of the CDK family." Liao J.C., Fisher R.P. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: 129/SvJ.
[2]	"Mouse p42(CCRK), cardiac variant." Qiu H., Depre C. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Heart.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY005133 mRNA. Translation: AAF89089.1. AY904369 mRNA. Translation: AAW82351.1. BC031907 mRNA. Translation: AAH31907.1.
IPI	IPI00119872. IPI00553299.
RefSeq	NP_444410.1.
UniGene	Mm.74982
3D structure databases
SMR	Q9JHU3. Positions 6-303.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9JHU3.
PTM databases
PhosphoSite	Q9JHU3.
Proteomic databases
PRIDE	Q9JHU3.
Genome annotation databases
Ensembl	ENSMUST00000021939; ENSMUSP00000021939; ENSMUSG00000021483; Mus musculus. [Genome view]
GeneID	105278.
KEGG	mmu:105278.
UCSC	uc007qyx.1. mouse.
Organism-specific databases
CTD	105278.
MGI	MGI:2145349. Cdk20.
Phylogenomic databases
eggNOG	roNOG11932.
HOGENOM	HBG755340.
HOVERGEN	Q9JHU3.
InParanoid	Q9JHU3.
OMA	QRIAASK.
PhylomeDB	Q9JHU3.
Enzyme and pathway databases
BRENDA	2.7.11.22. 244.
Gene expression databases
ArrayExpress	Q9JHU3.
Bgee	Q9JHU3.
CleanEx	MM_CCRK.
Genevestigator	Q9JHU3.
GermOnline	ENSMUSG00000021483. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	357580.
SOURCE	Search...

Entry information

Entry name

CDK20_MOUSE

Accession

Primary (citable) accession number: Q9JHU3
Secondary accession number(s): Q5EDC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)