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Protein

Cyclin-dependent kinase 20

Gene

Cdk20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160' (By similarity). Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 20 (EC:2.7.11.22)
Alternative name(s):
CDK-activating kinase p42
Short name:
CAK-kinase p42
CDK-related protein kinase PNQLARE
Cell cycle-related kinase
Cell division protein kinase 20
Cyclin-dependent protein kinase H
Cyclin-kinase-activating kinase p42
Gene namesi
Name:Cdk20
Synonyms:Ccrk, Cdch
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2145349. Cdk20.

Subcellular locationi

  1. Nucleus 1 Publication
  2. Cytoplasm 1 Publication
  3. Cell projectioncilium 1 Publication

GO - Cellular componenti

  1. cilium Source: UniProtKB-SubCell
  2. cytoplasm Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Cyclin-dependent kinase 20PRO_0000085703Add
BLAST

Proteomic databases

PaxDbiQ9JHU3.
PRIDEiQ9JHU3.

PTM databases

PhosphoSiteiQ9JHU3.

Expressioni

Gene expression databases

BgeeiQ9JHU3.
CleanExiMM_CCRK.
GenevestigatoriQ9JHU3.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with MAK (By similarity). Interacts with TBC1D32.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9JHU3.
SMRiQ9JHU3. Positions 1-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 288285Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119242.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ9JHU3.
KOiK08817.
OMAiAFTHGVP.
OrthoDBiEOG75J0NH.
PhylomeDBiQ9JHU3.
TreeFamiTF327240.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JHU3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDQYCILGRI GEGAHGIVFK AKHVETGEIV ALKKVALRRL EDGIPNQALR
60 70 80 90 100
EIKALQEIED SQYVVQLKAV FPHGAGFVLA FEFMLSDLAE VVRHAQRPLA
110 120 130 140 150
PAQVKSYLQM LLKGVAFCHA NNIVHRDLKP ANLLISASGQ LKIADFGLAR
160 170 180 190 200
VFSPDGGRLY THQVATRWYR APELLYGARQ YDQGVDLWAV GCIMGELLNG
210 220 230 240 250
SPLFPGENDI EQLCCVLRIL GTPSPRVWPE ITELPDYNKI SFKEQAPVPL
260 270 280 290 300
EEVLPDASPQ ALDLLGQFLL YPPRQRIAAS QALLHQYFFT APLPAHPSEL
310 320 330 340
PIPQRPGGPA PKAHPGPPHV HDFHVDRPLE ESLLNPELIR PFIPEG
Length:346
Mass (Da):38,379
Last modified:October 1, 2000 - v1
Checksum:iACCACE0C3FC4B833
GO
Isoform 2 (identifier: Q9JHU3-2) [UniParc]FASTAAdd to basket

Also known as: Cardiac CCRK

The sequence of this isoform differs from the canonical sequence as follows:
     168-243: WYRAPELLYG...LPDYNKISFK → SSLSCRTTTR...IHLSCRFLSV
     244-346: Missing.

Show »
Length:243
Mass (Da):26,974
Checksum:iB9EFCE73BAC18AC0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441I → F in AAW82351 (Ref. 2) Curated
Sequence conflicti58 – 581I → M in AAW82351 (Ref. 2) Curated
Sequence conflicti61 – 611S → N in AAW82351 (Ref. 2) Curated
Sequence conflicti75 – 751A → G in AAW82351 (Ref. 2) Curated
Sequence conflicti101 – 1011P → Q in AAW82351 (Ref. 2) Curated
Sequence conflicti157 – 1571G → S in AAW82351 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei168 – 24376WYRAP…KISFK → SSLSCRTTTRSPLRSRCPCP WRRCCLTSLPRHWICWVNSF STLLTSASQLPRLSSISTSS QLPCLPIHLSCRFLSV in isoform 2. 1 PublicationVSP_016753Add
BLAST
Alternative sequencei244 – 346103Missing in isoform 2. 1 PublicationVSP_016754Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY005133 mRNA. Translation: AAF89089.1.
AY904369 mRNA. Translation: AAW82351.1.
BC031907 mRNA. Translation: AAH31907.1.
CCDSiCCDS26601.1. [Q9JHU3-1]
RefSeqiNP_444410.1. NM_053180.2. [Q9JHU3-1]
UniGeneiMm.74982.

Genome annotation databases

EnsembliENSMUST00000021939; ENSMUSP00000021939; ENSMUSG00000021483. [Q9JHU3-1]
GeneIDi105278.
KEGGimmu:105278.
UCSCiuc007qyx.1. mouse. [Q9JHU3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY005133 mRNA. Translation: AAF89089.1.
AY904369 mRNA. Translation: AAW82351.1.
BC031907 mRNA. Translation: AAH31907.1.
CCDSiCCDS26601.1. [Q9JHU3-1]
RefSeqiNP_444410.1. NM_053180.2. [Q9JHU3-1]
UniGeneiMm.74982.

3D structure databases

ProteinModelPortaliQ9JHU3.
SMRiQ9JHU3. Positions 1-334.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9JHU3.

Proteomic databases

PaxDbiQ9JHU3.
PRIDEiQ9JHU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021939; ENSMUSP00000021939; ENSMUSG00000021483. [Q9JHU3-1]
GeneIDi105278.
KEGGimmu:105278.
UCSCiuc007qyx.1. mouse. [Q9JHU3-1]

Organism-specific databases

CTDi23552.
MGIiMGI:2145349. Cdk20.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119242.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ9JHU3.
KOiK08817.
OMAiAFTHGVP.
OrthoDBiEOG75J0NH.
PhylomeDBiQ9JHU3.
TreeFamiTF327240.

Enzyme and pathway databases

BRENDAi2.7.11.22. 3474.

Miscellaneous databases

NextBioi357580.
PROiQ9JHU3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JHU3.
CleanExiMM_CCRK.
GenevestigatoriQ9JHU3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PNQALRE a novel member of the CDK family."
    Liao J.C., Fisher R.P.
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: 129/SvJ.
  2. "Mouse p42(CCRK), cardiac variant."
    Qiu H., Depre C.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Heart.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye.
  4. "Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction."
    Ko H.W., Norman R.X., Tran J., Fuller K.P., Fukuda M., Eggenschwiler J.T.
    Dev. Cell 18:237-247(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TBC1D32, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCDK20_MOUSE
AccessioniPrimary (citable) accession number: Q9JHU3
Secondary accession number(s): Q5EDC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.