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Q9JHR7

- IDE_MOUSE

UniProt

Q9JHR7 - IDE_MOUSE

Protein

Insulin-degrading enzyme

Gene

Ide

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP By similarity.By similarity

    Catalytic activityi

    Degradation of insulin, glucagon and other polypeptides. No action on proteins.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Enzyme regulationi

    Activated by ATP, other nucleotide triphosphates and small peptides. Inhibited by bacitracin By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi108 – 1081ZincPROSITE-ProRule annotation
    Active sitei111 – 1111Proton acceptorPROSITE-ProRule annotation
    Metal bindingi112 – 1121ZincPROSITE-ProRule annotation
    Metal bindingi189 – 1891ZincPROSITE-ProRule annotation
    Binding sitei429 – 4291ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi895 – 9017ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. metalloendopeptidase activity Source: UniProtKB
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. beta-amyloid metabolic process Source: UniProtKB
    2. bradykinin catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Protein family/group databases

    MEROPSiM16.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Insulin-degrading enzyme (EC:3.4.24.56)
    Alternative name(s):
    Insulin protease
    Short name:
    Insulinase
    Insulysin
    Gene namesi
    Name:Ide
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:96412. Ide.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell membrane 1 Publication. Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. mitochondrion Source: MGI
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi853 – 8586Missing: Marked decrease in secretion. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10191019Insulin-degrading enzymePRO_0000074405Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei192 – 1921N6-succinyllysine1 Publication
    Modified residuei697 – 6971N6-succinyllysine1 Publication

    Proteomic databases

    MaxQBiQ9JHR7.
    PaxDbiQ9JHR7.
    PRIDEiQ9JHR7.

    PTM databases

    PhosphoSiteiQ9JHR7.

    Expressioni

    Inductioni

    Expression oscillates diurnally.1 Publication

    Gene expression databases

    CleanExiMM_IDE.
    GenevestigatoriQ9JHR7.

    Interactioni

    Subunit structurei

    Homodimer. Can form higher oligomers By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-60044N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JHR7.
    SMRiQ9JHR7. Positions 42-1011.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi853 – 8586SlyX motif

    Domaini

    The SlyX motif may be involved in the non-conventional secretion of the protein.

    Sequence similaritiesi

    Belongs to the peptidase M16 family.Curated

    Phylogenomic databases

    eggNOGiCOG1025.
    HOVERGENiHBG106799.
    InParanoidiQ9JHR7.

    Family and domain databases

    Gene3Di3.30.830.10. 4 hits.
    InterProiIPR011249. Metalloenz_LuxS/M16.
    IPR011237. Pept_M16_dom.
    IPR011765. Pept_M16_N.
    IPR001431. Pept_M16_Zn_BS.
    IPR007863. Peptidase_M16_C.
    [Graphical view]
    PfamiPF00675. Peptidase_M16. 1 hit.
    PF05193. Peptidase_M16_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF63411. SSF63411. 4 hits.
    PROSITEiPS00143. INSULINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JHR7-1 [UniParc]FASTAAdd to Basket

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    MRNGLVWLLH PALPGTLRSI LGARPPPAKR LCGFPKQTYS TMSNPAIQRI     50
    EDQIVKSPED KREYRGLELA NGIKVLLISD PTTDKSSAAL DVHIGSLSDP 100
    PNIPGLSHFC EHMLFLGTKK YPKENEYSQF LSEHAGSSNA FTSGEHTNYY 150
    FDVSHEHLEG ALDRFAQFFL CPLLDASCKD REVNAVDSEH EKNVMNDAWR 200
    LFQLEKATGN PKHPFSKFGT GNKYTLETRP NQEGIDVREE LLKFHSTYYS 250
    SNLMAICVLG RESLDDLTNL VVKLFSEVEN KNVPLPEFPE HPFQEEHLRQ 300
    LYKIVPIKDI RNLYVTFPIP DLQQYYKSNP GYYLGHLIGH EGPGSLLSEL 350
    KSKGWVNTLV GGQKEGARGF MFFIINVDLT EEGLLHVEDI ILHMFQYIQK 400
    LRAEGPQEWV FQECKDLNAV AFRFKDKERP RGYTSKIAGK LHYYPLNGVL 450
    TAEYLLEEFR PDLIDMVLDK LRPENVRVAI VSKSFEGKTD RTEQWYGTQY 500
    KQEAIPEDVI QKWQNADLNG KFKLPTKNEF IPTNFEILSL EKDATPYPAL 550
    IKDTAMSKLW FKQDDKFFLP KACLNFEFFS PFAYVDPLHC NMAYLYLELL 600
    KDSLNEYAYA AELAGLSYDL QNTIYGMYLS VKRYNDKQPI LLKKITEKMA 650
    TFEIDKKRFE IIKEAYMRSL NNFRAEQPHQ HAMYYLRLLM TEVAWTKDEL 700
    KEALDDVTLP RLKAFIPQLL SRLHIEALLH GNITKQAALG VMQMVEDTLI 750
    EHAHTKPLLP SQLVRYREVQ LPDRGWFVYQ QRNEVHNNCG IEIYYQTDMQ 800
    STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA NGIQGLRFII 850
    QSEKPPHYLE SRVEAFLITM EKAIEDMTEE AFQKHIQALA IRRLDKPKKL 900
    SAECAKYWGE IISQQYNYDR DNIEVAYLKT LTKDDIIRFY QEMLAVDAPR 950
    RHKVSVHVLA REMDSCPVVG EFPSQNDINL SEAPPLPQPE VIHNMTEFKR 1000
    GLPLFPLVKP HINFMAAKL 1019
    Length:1,019
    Mass (Da):117,772
    Last modified:October 1, 2000 - v1
    Checksum:i9443A6886110BE86
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ278422 mRNA. Translation: CAC01233.1.
    UniGeneiMm.28366.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ278422 mRNA. Translation: CAC01233.1 .
    UniGenei Mm.28366.

    3D structure databases

    ProteinModelPortali Q9JHR7.
    SMRi Q9JHR7. Positions 42-1011.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60044N.

    Protein family/group databases

    MEROPSi M16.002.

    PTM databases

    PhosphoSitei Q9JHR7.

    Proteomic databases

    MaxQBi Q9JHR7.
    PaxDbi Q9JHR7.
    PRIDEi Q9JHR7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:96412. Ide.

    Phylogenomic databases

    eggNOGi COG1025.
    HOVERGENi HBG106799.
    InParanoidi Q9JHR7.

    Miscellaneous databases

    ChiTaRSi IDE. mouse.
    PROi Q9JHR7.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_IDE.
    Genevestigatori Q9JHR7.

    Family and domain databases

    Gene3Di 3.30.830.10. 4 hits.
    InterProi IPR011249. Metalloenz_LuxS/M16.
    IPR011237. Pept_M16_dom.
    IPR011765. Pept_M16_N.
    IPR001431. Pept_M16_Zn_BS.
    IPR007863. Peptidase_M16_C.
    [Graphical view ]
    Pfami PF00675. Peptidase_M16. 1 hit.
    PF05193. Peptidase_M16_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF63411. SSF63411. 4 hits.
    PROSITEi PS00143. INSULINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Search for PTS1-containing protein in mammals."
      Van Veldhoven P.P.
      Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Functional relevance of a novel SlyX motif in non-conventional secretion of insulin-degrading enzyme."
      Glebov K., Schutze S., Walter J.
      J. Biol. Chem. 286:22711-22715(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION OF SLYX MOTIF, MUTAGENESIS OF 853-GLU--TYR-858.
    3. "Loss of mPer2 increases plasma insulin levels by enhanced glucose-stimulated insulin secretion and impaired insulin clearance in mice."
      Zhao Y., Zhang Y., Zhou M., Wang S., Hua Z., Zhang J.
      FEBS Lett. 586:1306-1311(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-192 AND LYS-697, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiIDE_MOUSE
    AccessioniPrimary (citable) accession number: Q9JHR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    ATP-binding induces a conformation change.By similarity

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3